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The map label for this gene is phaB [C]

Identifier: 52143120

GI number: 52143120

Start: 2239555

End: 2240610

Strand: Reverse

Name: phaB [C]

Synonym: BCZK2118

Alternate gene names: 52143120

Gene position: 2240610-2239555 (Counterclockwise)

Preceding gene: 52143112

Following gene: 52143121

Centisome position: 42.27

GC content: 34.19

Gene sequence:

>1056_bases
ATGACTGAACACGTTTTATTTTCTGTTAGTGAAAACGGCGTTGCATCAATTACTTTAAACCGCCCAAAAGCACTTAATTC
TTTATCTTATGAAATGTTGCAACCAATTGGGAAAAAACTAAAAGAGTGGGAAAATGATGAGCAAATTGCGCTAATTGTAT
TAAAAGGAGCTGGCACAAAAGGTTTTTGTGCAGGCGGTGACATTAAAACTCTTTATGAAGCACGTTCAAATGAAATTGCA
TTACAGCATGCGGAACGTTTCTTTGAAGAAGAGTATGAAATTGATACATATATTTATCAATACAAAAAACCGATTATCGC
TTGTTTAGATGGAATTGTAATGGGTGGTGGTGTTGGTCTTACAAATGGTGCGAAATACCGAATTGTAACCGAGCGTACAA
AATGGGCAATGCCAGAAATGAATATCGGCTTCTTCCCAGACGTCGGTGCTGCTTATTTCTTAAATAAAGCTCCAGGGTTC
GCTGGCAGATATGTTGCATTAACAGCATCTATTTTAAAAGCTTCTGACGTATTATTTATAAACGCTGCTGATTACTTTAT
GACATCTGATTCATTACCAAAGTTCCTTACTGAACTGGAAAGTGTAAATTGGCATAAAGGAGACGACGTACATATTCATT
TAAAAGAAGTTATTCGTACATTTGCAACTACTTCAAATTTAGAAAGCGAGCTTGCTCCATTATTAGAAGAAATCAATGCA
CATTTTGCTTTCGATACAATTGAAGAAATTATTCATTCATTAGAGAAAGATCAAAGTTCCTTTGCTTTAAAAACGAAAAA
AACACTATTATCAAAATCCCCTATTTCATTAAAAGTAACATTAAAACAGTTTATTGATGGACATGATAAGTCCGTTGAAG
AATGTTTCGCTACTGACCTTGTACTTGCTAAGAATTTCATGAGACATGAAGATTTCTTTGAAGGAGTACGATCCGTTGTC
GTTGATAAAGATCAAAATCCCAATTATAAATATAAGCAGTTAAGTGATGTTTCAGATGAAGATGTAAATCGGTTCTTTAA
CTTACTTAACGCATAA

Upstream 100 bases:

>100_bases
CAAATTAACACGTACCGCCAACTTTAGACAAAATTTATAAAGGACATTTCAGTACGTATCAAGAATTAAGAATAAGAAGA
TATATAAGGGAGGATGAATT

Downstream 100 bases:

>100_bases
AGTGAAACGTTAATTAGTGGGGTTCATACAGGAAAAAAATCGAGGCTACAGAGTCGTATAATTACACGGCTCTATAGCCT
CTTTATTATTTAATATTTTT

Product: 3-hydroxyisobutyryl-CoA hydrolase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTEHVLFSVSENGVASITLNRPKALNSLSYEMLQPIGKKLKEWENDEQIALIVLKGAGTKGFCAGGDIKTLYEARSNEIA
LQHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGLTNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGF
AGRYVALTASILKASDVLFINAADYFMTSDSLPKFLTELESVNWHKGDDVHIHLKEVIRTFATTSNLESELAPLLEEINA
HFAFDTIEEIIHSLEKDQSSFALKTKKTLLSKSPISLKVTLKQFIDGHDKSVEECFATDLVLAKNFMRHEDFFEGVRSVV
VDKDQNPNYKYKQLSDVSDEDVNRFFNLLNA

Sequences:

>Translated_351_residues
MTEHVLFSVSENGVASITLNRPKALNSLSYEMLQPIGKKLKEWENDEQIALIVLKGAGTKGFCAGGDIKTLYEARSNEIA
LQHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGLTNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGF
AGRYVALTASILKASDVLFINAADYFMTSDSLPKFLTELESVNWHKGDDVHIHLKEVIRTFATTSNLESELAPLLEEINA
HFAFDTIEEIIHSLEKDQSSFALKTKKTLLSKSPISLKVTLKQFIDGHDKSVEECFATDLVLAKNFMRHEDFFEGVRSVV
VDKDQNPNYKYKQLSDVSDEDVNRFFNLLNA
>Mature_350_residues
TEHVLFSVSENGVASITLNRPKALNSLSYEMLQPIGKKLKEWENDEQIALIVLKGAGTKGFCAGGDIKTLYEARSNEIAL
QHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGLTNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGFA
GRYVALTASILKASDVLFINAADYFMTSDSLPKFLTELESVNWHKGDDVHIHLKEVIRTFATTSNLESELAPLLEEINAH
FAFDTIEEIIHSLEKDQSSFALKTKKTLLSKSPISLKVTLKQFIDGHDKSVEECFATDLVLAKNFMRHEDFFEGVRSVVV
DKDQNPNYKYKQLSDVSDEDVNRFFNLLNA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=339, Percent_Identity=40.7079646017699, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI37594469, Length=295, Percent_Identity=39.6610169491525, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI194097323, Length=148, Percent_Identity=34.4594594594595, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI68989263, Length=182, Percent_Identity=28.021978021978, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI221142681, Length=149, Percent_Identity=30.2013422818792, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788597, Length=258, Percent_Identity=23.2558139534884, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787660, Length=174, Percent_Identity=32.7586206896552, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=337, Percent_Identity=39.1691394658754, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI25144160, Length=337, Percent_Identity=39.1691394658754, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI17554946, Length=146, Percent_Identity=33.5616438356164, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=156, Percent_Identity=33.974358974359, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=187, Percent_Identity=23.5294117647059, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=179, Percent_Identity=25.6983240223464, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=159, Percent_Identity=28.3018867924528, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=350, Percent_Identity=32.8571428571429, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI28571730, Length=333, Percent_Identity=41.1411411411411, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI28571729, Length=333, Percent_Identity=41.1411411411411, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI20129971, Length=226, Percent_Identity=27.8761061946903, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24653477, Length=226, Percent_Identity=27.8761061946903, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24653139, Length=177, Percent_Identity=27.1186440677966, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 39525; Mature: 39394

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHVLFSVSENGVASITLNRPKALNSLSYEMLQPIGKKLKEWENDEQIALIVLKGAGTK
CCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
GFCAGGDIKTLYEARSNEIALQHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGL
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGFAGRYVALTASILKASDVLFI
CCCCEEEEEECCCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEE
NAADYFMTSDSLPKFLTELESVNWHKGDDVHIHLKEVIRTFATTSNLESELAPLLEEINA
ECHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHH
HFAFDTIEEIIHSLEKDQSSFALKTKKTLLSKSPISLKVTLKQFIDGHDKSVEECFATDL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCCHHHHHHHHHH
VLAKNFMRHEDFFEGVRSVVVDKDQNPNYKYKQLSDVSDEDVNRFFNLLNA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TEHVLFSVSENGVASITLNRPKALNSLSYEMLQPIGKKLKEWENDEQIALIVLKGAGTK
CCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
GFCAGGDIKTLYEARSNEIALQHAERFFEEEYEIDTYIYQYKKPIIACLDGIVMGGGVGL
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TNGAKYRIVTERTKWAMPEMNIGFFPDVGAAYFLNKAPGFAGRYVALTASILKASDVLFI
CCCCEEEEEECCCHHCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEE
NAADYFMTSDSLPKFLTELESVNWHKGDDVHIHLKEVIRTFATTSNLESELAPLLEEINA
ECHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHH
HFAFDTIEEIIHSLEKDQSSFALKTKKTLLSKSPISLKVTLKQFIDGHDKSVEECFATDL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCCHHHHHHHHHH
VLAKNFMRHEDFFEGVRSVVVDKDQNPNYKYKQLSDVSDEDVNRFFNLLNA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA