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The map label for this gene is ykfC [H]

Identifier: 52142663

GI number: 52142663

Start: 2698397

End: 2699398

Strand: Reverse

Name: ykfC [H]

Synonym: BCZK2577

Alternate gene names: 52142663

Gene position: 2699398-2698397 (Counterclockwise)

Preceding gene: 52142662

Following gene: 52142665

Centisome position: 50.92

GC content: 38.52

Gene sequence:

>1002_bases
ATGAAAAAAGTAGGAACTGCATTTTTAACAACTTTATTTATATTTTCATCGTTTACATCAGCAAATGCTGAAGAAAAGAA
AGATAGTAAGGCGTTTATCGATGTATCTGCTGCAACACTTTGGACCACGCCGGATTCATTACGGCCAATTGATGCACCAA
GTGCTACAAATCCAGTTGATTTGTGGAAGTGGACGAAATCGATGACGCTTGATGAGAAACTTTGGTTAACAAGTGCAAAT
AAATTAGAAACGCAAGCGTTATTAGGTCAAGAAGTAACAGTTGTAGATAAAAAAGGGGATTGGGTGAAAGTGTTAGTCCA
TGGTCAGCCAACACCTCGAAATGAAGAAGGCTACCCAGGATGGATGCCTGAAAAACAGTTAACATATAATCAAGAATTTG
CAGATAAAACAAATGAACCTTTCGTATTAGTAACAAAACCGACAGCCATTTTATATATAAATCCTTCTGAAAAACATAAA
TCGCTTGAAGTGAGCTATAATACGCGACTGCCGCTACTTAGTGAAGATACGATTTCATACCGCGTGTTGCTACCAAATGG
GCAGAAAGCTTGGCTACGAAAAAATGATGGAACGGTTTACCGTTCTCAAAATGATATTCCAACCCCGGCGGCCGATGATT
TAATAAACACAGGGAAAATGTTTTTAGGATTACCGTACATTTGGGCTGGTACAAGTGGCTTCGGATTTGATTGCTCTGGA
TTTACACATACGATTTATAAATCGCACGGTATTACAATTCCACGAGATTCTGGTCCGCAATCGAGAAATGGAGTTGCAGT
GGATAAAGAAAATTTACAAAAAGGAGATTTAATCTTCTTTGCACATGATCAAGGGAAAGGTAGTGTCCATCATGTGGGAA
TGTATATTGGTGACGGAAATATGATTCACTCACCAAGAGCAGAAAGATCAGTAGAGATTATACCGTTAAATACACCTGGA
TATATAGAAGAATACGCTGGTGCTCGTCGTTACTTACCTTAA

Upstream 100 bases:

>100_bases
AGGAATGACTTATTCTGGATCAAAGGTAAGCCTTCAATCGACAGTGAATAGTAAATCTTAGGGGAAGAGACTAGTAAATT
ATCTTTTAAGGGGGATATTT

Downstream 100 bases:

>100_bases
AAAATAGAGGGGGTTTTCAAATGAAAAAATTTGTTCGCTACTCATTAGTTAGTACTCTATTCGTTTCTTCGATTTTAGTT
GGCTGCGCAAAAGAAAAAAC

Product: cell wall-associated hydrolase

Products: NA

Alternate protein names: Cell wall endopeptidase ykfC [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKKVGTAFLTTLFIFSSFTSANAEEKKDSKAFIDVSAATLWTTPDSLRPIDAPSATNPVDLWKWTKSMTLDEKLWLTSAN
KLETQALLGQEVTVVDKKGDWVKVLVHGQPTPRNEEGYPGWMPEKQLTYNQEFADKTNEPFVLVTKPTAILYINPSEKHK
SLEVSYNTRLPLLSEDTISYRVLLPNGQKAWLRKNDGTVYRSQNDIPTPAADDLINTGKMFLGLPYIWAGTSGFGFDCSG
FTHTIYKSHGITIPRDSGPQSRNGVAVDKENLQKGDLIFFAHDQGKGSVHHVGMYIGDGNMIHSPRAERSVEIIPLNTPG
YIEEYAGARRYLP

Sequences:

>Translated_333_residues
MKKVGTAFLTTLFIFSSFTSANAEEKKDSKAFIDVSAATLWTTPDSLRPIDAPSATNPVDLWKWTKSMTLDEKLWLTSAN
KLETQALLGQEVTVVDKKGDWVKVLVHGQPTPRNEEGYPGWMPEKQLTYNQEFADKTNEPFVLVTKPTAILYINPSEKHK
SLEVSYNTRLPLLSEDTISYRVLLPNGQKAWLRKNDGTVYRSQNDIPTPAADDLINTGKMFLGLPYIWAGTSGFGFDCSG
FTHTIYKSHGITIPRDSGPQSRNGVAVDKENLQKGDLIFFAHDQGKGSVHHVGMYIGDGNMIHSPRAERSVEIIPLNTPG
YIEEYAGARRYLP
>Mature_333_residues
MKKVGTAFLTTLFIFSSFTSANAEEKKDSKAFIDVSAATLWTTPDSLRPIDAPSATNPVDLWKWTKSMTLDEKLWLTSAN
KLETQALLGQEVTVVDKKGDWVKVLVHGQPTPRNEEGYPGWMPEKQLTYNQEFADKTNEPFVLVTKPTAILYINPSEKHK
SLEVSYNTRLPLLSEDTISYRVLLPNGQKAWLRKNDGTVYRSQNDIPTPAADDLINTGKMFLGLPYIWAGTSGFGFDCSG
FTHTIYKSHGITIPRDSGPQSRNGVAVDKENLQKGDLIFFAHDQGKGSVHHVGMYIGDGNMIHSPRAERSVEIIPLNTPG
YIEEYAGARRYLP

Specific function: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=115, Percent_Identity=39.1304347826087, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786421, Length=115, Percent_Identity=38.2608695652174, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788501, Length=111, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 37141; Mature: 37141

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVGTAFLTTLFIFSSFTSANAEEKKDSKAFIDVSAATLWTTPDSLRPIDAPSATNPVD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCHH
LWKWTKSMTLDEKLWLTSANKLETQALLGQEVTVVDKKGDWVKVLVHGQPTPRNEEGYPG
HHHHHHCCCCCCEEEEECCCHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCC
WMPEKQLTYNQEFADKTNEPFVLVTKPTAILYINPSEKHKSLEVSYNTRLPLLSEDTISY
CCCCCCCCCCHHHHCCCCCCEEEEECCEEEEEECCCCCCCEEEEEECCCCCEEECCCEEE
RVLLPNGQKAWLRKNDGTVYRSQNDIPTPAADDLINTGKMFLGLPYIWAGTSGFGFDCSG
EEEECCCCEEEEECCCCEEEECCCCCCCCCHHHHHHCCHHHEECCEEEECCCCCCCCCCC
FTHTIYKSHGITIPRDSGPQSRNGVAVDKENLQKGDLIFFAHDQGKGSVHHVGMYIGDGN
CEEEEEECCCEECCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCEEEEEEEEECCC
MIHSPRAERSVEIIPLNTPGYIEEYAGARRYLP
EEECCCCCCCEEEEECCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MKKVGTAFLTTLFIFSSFTSANAEEKKDSKAFIDVSAATLWTTPDSLRPIDAPSATNPVD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCHH
LWKWTKSMTLDEKLWLTSANKLETQALLGQEVTVVDKKGDWVKVLVHGQPTPRNEEGYPG
HHHHHHCCCCCCEEEEECCCHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCC
WMPEKQLTYNQEFADKTNEPFVLVTKPTAILYINPSEKHKSLEVSYNTRLPLLSEDTISY
CCCCCCCCCCHHHHCCCCCCEEEEECCEEEEEECCCCCCCEEEEEECCCCCEEECCCEEE
RVLLPNGQKAWLRKNDGTVYRSQNDIPTPAADDLINTGKMFLGLPYIWAGTSGFGFDCSG
EEEECCCCEEEEECCCCEEEECCCCCCCCCHHHHHHCCHHHEECCEEEECCCCCCCCCCC
FTHTIYKSHGITIPRDSGPQSRNGVAVDKENLQKGDLIFFAHDQGKGSVHHVGMYIGDGN
CEEEEEECCCEECCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCEEEEEEEEECCC
MIHSPRAERSVEIIPLNTPGYIEEYAGARRYLP
EEECCCCCCCEEEEECCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]