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The map label for this gene is ykfB [H]

Identifier: 52142662

GI number: 52142662

Start: 2699438

End: 2700490

Strand: Reverse

Name: ykfB [H]

Synonym: BCZK2578

Alternate gene names: 52142662

Gene position: 2700490-2699438 (Counterclockwise)

Preceding gene: 52142661

Following gene: 52142663

Centisome position: 50.94

GC content: 38.18

Gene sequence:

>1053_bases
ATGAAAATTACCGATGTAAAAGTAAATCGTAGACGTGTTAATCTTCATACACCGTTTAAAACCGCGCTTCGTACCGTAAC
CGAAATAGAAAGTATAGATGTATATATTCATACAGATGAAGGAATTGTCGGTAAGGGAGCTGCAGCTGCAACGCCGGTTA
TTACGGGTGATTTCGCTAACGGGATTGAAGAAGCAATTTTAGGGCCGATGCGTTCATGTTTAATTGGTCAAGATATCATT
CAATTTCAGCAGTTACTGCAGCACATTCAAATGAGTTGCATTGGAAATCCAAGTGCGAAAGCAGCGGTAGATATCGCATT
ATATGATGTGTATTGTCAATATCGTAACGTTCCACTTTACGCATTATTAGGCGGGAAGAAAGAAATTCATACAGATATTA
CGGTGAGTGTAGATGAGCCTTTCATTATGGCGAAAGAGGCAAAGCAACATGTAGAAAAAGGATTTCAAACTTTAAAAATT
AAAGTGGGGAAATCTGCACACTTAGACTTAGAACGTATTGAAGCGATTCGAAATAGCATACCAAAAAATACGACGTTACG
ATTAGATGCAAATCAAGGGTGGAATCCAAAAGAGGCAGTTTCTATCATTAAAGAGATGGAAAATCGCAATTTAAATATTG
AATTTATTGAACAACCTGTTCATGCGAAAGATTGGGATGGATTAAAGTACGTCAAAGATCATGTACAAACACCTATTATG
GCAGATGAAAGCATATTTTCAGCGAGTGATGCATTAAAAATTGTGCAAGGAAGATATGCAGACTTAATCAATATTAAATT
AATGAAATGTGGTGGCATTCGCGAAGCGTGGCGTATTGCCGATATCGCAGAAGCAGCTGGTGTGAAATGTATGGTAGGAA
GCATGATGGAATCTTCTCTTTCTGTTAGCGCTGTTGCACATTTAGCTGCTGCACATCCTAATATTCATTACTTCGATCTT
GATGCGCCACTTTGGTTAATGGAAGAACCGGAAGGAATGACTTATTCTGGATCAAAGGTAAGCCTTCAATCGACAGTGAA
TAGTAAATCTTAG

Upstream 100 bases:

>100_bases
GTACCTTCTACATGGAAAGTAAGCACAAGCGGAGATCTTTTGATCCAAAAAGGTTAAGAATTAAGGATCCATTTTGCTAG
ACAAAGAAAGGATGAACAAA

Downstream 100 bases:

>100_bases
GGGAAGAGACTAGTAAATTATCTTTTAAGGGGGATATTTATGAAAAAAGTAGGAACTGCATTTTTAACAACTTTATTTAT
ATTTTCATCGTTTACATCAG

Product: mandelate racemase/muconate lactonizing enzyme

Products: (R)-mandelate

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAAAATPVITGDFANGIEEAILGPMRSCLIGQDII
QFQQLLQHIQMSCIGNPSAKAAVDIALYDVYCQYRNVPLYALLGGKKEIHTDITVSVDEPFIMAKEAKQHVEKGFQTLKI
KVGKSAHLDLERIEAIRNSIPKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYVKDHVQTPIM
ADESIFSASDALKIVQGRYADLINIKLMKCGGIREAWRIADIAEAAGVKCMVGSMMESSLSVSAVAHLAAAHPNIHYFDL
DAPLWLMEEPEGMTYSGSKVSLQSTVNSKS

Sequences:

>Translated_350_residues
MKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAAAATPVITGDFANGIEEAILGPMRSCLIGQDII
QFQQLLQHIQMSCIGNPSAKAAVDIALYDVYCQYRNVPLYALLGGKKEIHTDITVSVDEPFIMAKEAKQHVEKGFQTLKI
KVGKSAHLDLERIEAIRNSIPKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYVKDHVQTPIM
ADESIFSASDALKIVQGRYADLINIKLMKCGGIREAWRIADIAEAAGVKCMVGSMMESSLSVSAVAHLAAAHPNIHYFDL
DAPLWLMEEPEGMTYSGSKVSLQSTVNSKS
>Mature_350_residues
MKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAAAATPVITGDFANGIEEAILGPMRSCLIGQDII
QFQQLLQHIQMSCIGNPSAKAAVDIALYDVYCQYRNVPLYALLGGKKEIHTDITVSVDEPFIMAKEAKQHVEKGFQTLKI
KVGKSAHLDLERIEAIRNSIPKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYVKDHVQTPIM
ADESIFSASDALKIVQGRYADLINIKLMKCGGIREAWRIADIAEAAGVKCMVGSMMESSLSVSAVAHLAAAHPNIHYFDL
DAPLWLMEEPEGMTYSGSKVSLQSTVNSKS

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=323, Percent_Identity=31.5789473684211, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI48994953, Length=307, Percent_Identity=29.3159609120521, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 5.1.2.2

Molecular weight: Translated: 38698; Mature: 38698

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAAAATPVITGDFAN
CCEEEEEECCEEEEECCCHHHHHHHHHHHEEEEEEEECCCCCEECCCCCCCCEEECHHHH
GIEEAILGPMRSCLIGQDIIQFQQLLQHIQMSCIGNPSAKAAVDIALYDVYCQYRNVPLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEHHHCCCCEE
ALLGGKKEIHTDITVSVDEPFIMAKEAKQHVEKGFQTLKIKVGKSAHLDLERIEAIRNSI
EEECCCEEECCCEEEEECCCEEEHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCC
PKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYVKDHVQTPIM
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCC
ADESIFSASDALKIVQGRYADLINIKLMKCGGIREAWRIADIAEAAGVKCMVGSMMESSL
CCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
SVSAVAHLAAAHPNIHYFDLDAPLWLMEEPEGMTYSGSKVSLQSTVNSKS
HHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCEECCCEEEEECCCCCCC
>Mature Secondary Structure
MKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAAAATPVITGDFAN
CCEEEEEECCEEEEECCCHHHHHHHHHHHEEEEEEEECCCCCEECCCCCCCCEEECHHHH
GIEEAILGPMRSCLIGQDIIQFQQLLQHIQMSCIGNPSAKAAVDIALYDVYCQYRNVPLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEHHHCCCCEE
ALLGGKKEIHTDITVSVDEPFIMAKEAKQHVEKGFQTLKIKVGKSAHLDLERIEAIRNSI
EEECCCEEECCCEEEEECCCEEEHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCC
PKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYVKDHVQTPIM
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCC
ADESIFSASDALKIVQGRYADLINIKLMKCGGIREAWRIADIAEAAGVKCMVGSMMESSL
CCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
SVSAVAHLAAAHPNIHYFDLDAPLWLMEEPEGMTYSGSKVSLQSTVNSKS
HHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCEECCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-mandelate

Specific reaction: (S)-mandelate = (R)-mandelate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]