Click here to switch to the map view.

The map label for this gene is pbp2 [H]

Identifier: 52142440

GI number: 52142440

Start: 2916976

End: 2919129

Strand: Reverse

Name: pbp2 [H]

Synonym: BCZK2802

Alternate gene names: 52142440

Gene position: 2919129-2916976 (Counterclockwise)

Preceding gene: 52142436

Following gene: 52142441

Centisome position: 55.07

GC content: 36.26

Gene sequence:

>2154_bases
ATGAGACAGAAAAAGGAGCAGAAGAAACAATCGAAAAAGAAAAAGACTCATATTCCTTTTCGGTTAAATGTACTATTTTT
TATTGTCTTTCTTTTATTTTCAGCTATTATAATTCAATTAGGTAAAGTGCAAATCATTGATGGAGAAACGTATAGAAATG
AAGTGAACAAAAAGGAAGACGTAACGGTAAGTACACCTGTTCCTAGAGGGAAAATGTTTGATCGACAAGGTCAGGTGATT
GTAAATAACAAACCACTACGAACCGTTACATATACGAAAATGAAAGGAGTAGATTCGAAAGAAGTTTTACAAGTAGCAAG
ACAGTTAGCCCAGTTAATTGAAATGTCGCCAGAGGATATAGACAAGTTAACAGAAACAGATAAAAAAGATTTTTGGATGC
AGTTAAATGAAAAGCGGGCAGCAGCAAAAGTAACGAAAGAAGATGAGAGTAAATTTAGGAAGCAAGAGATAGAAGGGAAA
GAATTAGATAAAAAAGTGGAAGAGTTACGTAGGGAAAGAATTACACAAGAAGAATTAAGGGAACTTTCAAAAGAGGATCT
AGAAGTACTAGCAATTAAAAGCAAAATGAATGCGGGATATAAAATGACGCCGCAAATTGTTAAAAAAGATGTAAGTCAAA
ATGAATATGCGGTCGTAAGTGAAAGGTTGGCAAGCTTGCCTGGAGTAGATACGACAGTGGATTGGGAACGTGAATACCCA
AATGATAAAATACTTCGTTCTATCCTTGGTAGCGTTTCTACTGAAAATGAAGGATTGCCAAGGGAACAATTAGATTATTA
TTTAGTGCGTGATTATAACCGAAATGACCGTATTGGTAAGAGCTACATCGAAAAGCAATATGAAGACGCATTACATGGAA
CGAAAGAACAGTCTAGAAGCATTACAGATAAAGCAGGAAATATTATTCGGACAGAAAAAGTAACTGAAGGAAAAAGTGGA
AATAATTTAATGTTAACAGTGGATATGGAGTTACAGAAAAAAGTAGAGGGAAGTCTTGAAAAGAATTTACGAGCATTTCA
TTCTGCAGAGCCGATGATGGATCGTGCATTTGTTGTAATGATGAATCCGAAAAACGGTCAAATTTTATCGATGGCAGGAA
AGAAAATTGTAAATAAAGATGGCGGGATGCAAATAGAAGACTATGCTTTAGGGACGATGACAAGTTCTTATGAGTTAGGG
TCAACTGTGAAAGGGGCTACATTATTAGCCGGCTATCAAACTGACACAATTAAGCCGTATACACATTTCTTTGATGCACC
CATGTATTTTAAAGGAAGCTCTAAGCCGAAGAAATCGTGGAAAGACTTTGGTGATATTGATGATTTAAGAGCTTTACAAG
TTTCATCAAACGTATATATGTTTAATACAGCATTAAAAATGGCAGGTATAAATTATGTGCCAAACAATCCATTAGACATT
AAACAAGAAACATTTAATAAAATGCGCTACTATTTTAGACAATTTGGTTTAGGTGTACCGACTGGTATTGACTTGCCGAA
TGAATCGATTGGTCAAATGGGTAGAACGGATAACATACCGGGTTTTTTACTTGATTATGCGATTGGACAGTACGATACAT
ATACGCCGCTTCAACTTGCTCAATATATTTCAACCATTGCAAACGGTGGTTACAGAATGAAACCGCAAATTGTGCAAGAA
GTGAGAGAGCAACCACATAGACCAGAGGATGTTGGAAAAGTTATTCGATCTATTGAACCAGTTATTTTAAATCGAGTGGA
TATGAACACTTCATACATTAATCATGTAAAAGAAGGATTTAGAAGAGTTTTTCAAGAGAATGACGGAACAGGTACTGCAA
CGTTTAAAGGATTACCGTATAAACCAGCTGGAAAAACAGGGACAGCTGAAACTGTGTATGGCGGAGAAAGTGAAATTGGA
AGAGATGAAAACAATAATCGAAAAAAATGTTATAATTTAACTCTTGCCGGTTATGCGCCGTATGATGCTGATCCGGAAGT
AGCTTTTTCAGTTGTTGTACCATGGGTGAATGATGACAAATCAGGAATTAATTCCGCGATTGGTAAAGAGATTTTGGATG
CGTATTTTGAATTGAAAGCGAAAAGAGCAGGTGGAAATCCAGTCCCAGTAGAGCAGCCGAACAAGGCCCAGTAA

Upstream 100 bases:

>100_bases
CTTTTGTAATTAATATAAAATTCTATATATATCATATAAATAAATTTGATACATAATGGTTACATGTAGAAATGTTAATA
GAATGAAGCGAGGGAGCGAA

Downstream 100 bases:

>100_bases
AAATAGTATCACTGCATAGAGACATATGGTGCAGTGATATATTTTTTGATTTAAAAAGCGTTTGAATAAGGTGGGAATGG
ATATGAAACAGGAGATTAAG

Product: penicillin-binding protein 2

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: NA

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MRQKKEQKKQSKKKKTHIPFRLNVLFFIVFLLFSAIIIQLGKVQIIDGETYRNEVNKKEDVTVSTPVPRGKMFDRQGQVI
VNNKPLRTVTYTKMKGVDSKEVLQVARQLAQLIEMSPEDIDKLTETDKKDFWMQLNEKRAAAKVTKEDESKFRKQEIEGK
ELDKKVEELRRERITQEELRELSKEDLEVLAIKSKMNAGYKMTPQIVKKDVSQNEYAVVSERLASLPGVDTTVDWEREYP
NDKILRSILGSVSTENEGLPREQLDYYLVRDYNRNDRIGKSYIEKQYEDALHGTKEQSRSITDKAGNIIRTEKVTEGKSG
NNLMLTVDMELQKKVEGSLEKNLRAFHSAEPMMDRAFVVMMNPKNGQILSMAGKKIVNKDGGMQIEDYALGTMTSSYELG
STVKGATLLAGYQTDTIKPYTHFFDAPMYFKGSSKPKKSWKDFGDIDDLRALQVSSNVYMFNTALKMAGINYVPNNPLDI
KQETFNKMRYYFRQFGLGVPTGIDLPNESIGQMGRTDNIPGFLLDYAIGQYDTYTPLQLAQYISTIANGGYRMKPQIVQE
VREQPHRPEDVGKVIRSIEPVILNRVDMNTSYINHVKEGFRRVFQENDGTGTATFKGLPYKPAGKTGTAETVYGGESEIG
RDENNNRKKCYNLTLAGYAPYDADPEVAFSVVVPWVNDDKSGINSAIGKEILDAYFELKAKRAGGNPVPVEQPNKAQ

Sequences:

>Translated_717_residues
MRQKKEQKKQSKKKKTHIPFRLNVLFFIVFLLFSAIIIQLGKVQIIDGETYRNEVNKKEDVTVSTPVPRGKMFDRQGQVI
VNNKPLRTVTYTKMKGVDSKEVLQVARQLAQLIEMSPEDIDKLTETDKKDFWMQLNEKRAAAKVTKEDESKFRKQEIEGK
ELDKKVEELRRERITQEELRELSKEDLEVLAIKSKMNAGYKMTPQIVKKDVSQNEYAVVSERLASLPGVDTTVDWEREYP
NDKILRSILGSVSTENEGLPREQLDYYLVRDYNRNDRIGKSYIEKQYEDALHGTKEQSRSITDKAGNIIRTEKVTEGKSG
NNLMLTVDMELQKKVEGSLEKNLRAFHSAEPMMDRAFVVMMNPKNGQILSMAGKKIVNKDGGMQIEDYALGTMTSSYELG
STVKGATLLAGYQTDTIKPYTHFFDAPMYFKGSSKPKKSWKDFGDIDDLRALQVSSNVYMFNTALKMAGINYVPNNPLDI
KQETFNKMRYYFRQFGLGVPTGIDLPNESIGQMGRTDNIPGFLLDYAIGQYDTYTPLQLAQYISTIANGGYRMKPQIVQE
VREQPHRPEDVGKVIRSIEPVILNRVDMNTSYINHVKEGFRRVFQENDGTGTATFKGLPYKPAGKTGTAETVYGGESEIG
RDENNNRKKCYNLTLAGYAPYDADPEVAFSVVVPWVNDDKSGINSAIGKEILDAYFELKAKRAGGNPVPVEQPNKAQ
>Mature_717_residues
MRQKKEQKKQSKKKKTHIPFRLNVLFFIVFLLFSAIIIQLGKVQIIDGETYRNEVNKKEDVTVSTPVPRGKMFDRQGQVI
VNNKPLRTVTYTKMKGVDSKEVLQVARQLAQLIEMSPEDIDKLTETDKKDFWMQLNEKRAAAKVTKEDESKFRKQEIEGK
ELDKKVEELRRERITQEELRELSKEDLEVLAIKSKMNAGYKMTPQIVKKDVSQNEYAVVSERLASLPGVDTTVDWEREYP
NDKILRSILGSVSTENEGLPREQLDYYLVRDYNRNDRIGKSYIEKQYEDALHGTKEQSRSITDKAGNIIRTEKVTEGKSG
NNLMLTVDMELQKKVEGSLEKNLRAFHSAEPMMDRAFVVMMNPKNGQILSMAGKKIVNKDGGMQIEDYALGTMTSSYELG
STVKGATLLAGYQTDTIKPYTHFFDAPMYFKGSSKPKKSWKDFGDIDDLRALQVSSNVYMFNTALKMAGINYVPNNPLDI
KQETFNKMRYYFRQFGLGVPTGIDLPNESIGQMGRTDNIPGFLLDYAIGQYDTYTPLQLAQYISTIANGGYRMKPQIVQE
VREQPHRPEDVGKVIRSIEPVILNRVDMNTSYINHVKEGFRRVFQENDGTGTATFKGLPYKPAGKTGTAETVYGGESEIG
RDENNNRKKCYNLTLAGYAPYDADPEVAFSVVVPWVNDDKSGINSAIGKEILDAYFELKAKRAGGNPVPVEQPNKAQ

Specific function: Cell Wall Formation; Pbp-2 Is Responsible For The Determination Of The Rod Shape Of The Cell. Its Synthesize Cross- Linked Peptidoglycan From The Lipid Intermediates. [C]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=714, Percent_Identity=23.9495798319328, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1786272, Length=462, Percent_Identity=24.8917748917749, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 81428; Mature: 81428

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQKKEQKKQSKKKKTHIPFRLNVLFFIVFLLFSAIIIQLGKVQIIDGETYRNEVNKKED
CCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHCCCCCC
VTVSTPVPRGKMFDRQGQVIVNNKPLRTVTYTKMKGVDSKEVLQVARQLAQLIEMSPEDI
EEEECCCCCCCCCCCCCCEEECCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCHHHH
DKLTETDKKDFWMQLNEKRAAAKVTKEDESKFRKQEIEGKELDKKVEELRRERITQEELR
HHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHH
ELSKEDLEVLAIKSKMNAGYKMTPQIVKKDVSQNEYAVVSERLASLPGVDTTVDWEREYP
HHHHHHHHEEEEHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEECCHHCCC
NDKILRSILGSVSTENEGLPREQLDYYLVRDYNRNDRIGKSYIEKQYEDALHGTKEQSRS
CHHHHHHHHHCCCCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
ITDKAGNIIRTEKVTEGKSGNNLMLTVDMELQKKVEGSLEKNLRAFHSAEPMMDRAFVVM
HHHHCCCEEEECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
MNPKNGQILSMAGKKIVNKDGGMQIEDYALGTMTSSYELGSTVKGATLLAGYQTDTIKPY
ECCCCCCEEECCCHHHHCCCCCCEEECEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHH
THFFDAPMYFKGSSKPKKSWKDFGDIDDLRALQVSSNVYMFNTALKMAGINYVPNNPLDI
HHHHCCCEEECCCCCCHHHHHCCCCHHHHHEEEECCCEEEEEHHHHHCCCCCCCCCCCCC
KQETFNKMRYYFRQFGLGVPTGIDLPNESIGQMGRTDNIPGFLLDYAIGQYDTYTPLQLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHH
QYISTIANGGYRMKPQIVQEVREQPHRPEDVGKVIRSIEPVILNRVDMNTSYINHVKEGF
HHHHHHHCCCEEECHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHH
RRVFQENDGTGTATFKGLPYKPAGKTGTAETVYGGESEIGRDENNNRKKCYNLTLAGYAP
HHHHHCCCCCCEEEECCCCCCCCCCCCCCCEECCCCHHCCCCCCCCCCEEEEEEEECCCC
YDADPEVAFSVVVPWVNDDKSGINSAIGKEILDAYFELKAKRAGGNPVPVEQPNKAQ
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRQKKEQKKQSKKKKTHIPFRLNVLFFIVFLLFSAIIIQLGKVQIIDGETYRNEVNKKED
CCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHCCCCCC
VTVSTPVPRGKMFDRQGQVIVNNKPLRTVTYTKMKGVDSKEVLQVARQLAQLIEMSPEDI
EEEECCCCCCCCCCCCCCEEECCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCHHHH
DKLTETDKKDFWMQLNEKRAAAKVTKEDESKFRKQEIEGKELDKKVEELRRERITQEELR
HHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHH
ELSKEDLEVLAIKSKMNAGYKMTPQIVKKDVSQNEYAVVSERLASLPGVDTTVDWEREYP
HHHHHHHHEEEEHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEECCHHCCC
NDKILRSILGSVSTENEGLPREQLDYYLVRDYNRNDRIGKSYIEKQYEDALHGTKEQSRS
CHHHHHHHHHCCCCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
ITDKAGNIIRTEKVTEGKSGNNLMLTVDMELQKKVEGSLEKNLRAFHSAEPMMDRAFVVM
HHHHCCCEEEECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
MNPKNGQILSMAGKKIVNKDGGMQIEDYALGTMTSSYELGSTVKGATLLAGYQTDTIKPY
ECCCCCCEEECCCHHHHCCCCCCEEECEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHH
THFFDAPMYFKGSSKPKKSWKDFGDIDDLRALQVSSNVYMFNTALKMAGINYVPNNPLDI
HHHHCCCEEECCCCCCHHHHHCCCCHHHHHEEEECCCEEEEEHHHHHCCCCCCCCCCCCC
KQETFNKMRYYFRQFGLGVPTGIDLPNESIGQMGRTDNIPGFLLDYAIGQYDTYTPLQLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHH
QYISTIANGGYRMKPQIVQEVREQPHRPEDVGKVIRSIEPVILNRVDMNTSYINHVKEGF
HHHHHHHCCCEEECHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHH
RRVFQENDGTGTATFKGLPYKPAGKTGTAETVYGGESEIGRDENNNRKKCYNLTLAGYAP
HHHHHCCCCCCEEEECCCCCCCCCCCCCCCEECCCCHHCCCCCCCCCCEEEEEEEECCCC
YDADPEVAFSVVVPWVNDDKSGINSAIGKEILDAYFELKAKRAGGNPVPVEQPNKAQ
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]