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The map label for this gene is aspA [H]

Identifier: 52142397

GI number: 52142397

Start: 2961794

End: 2963224

Strand: Reverse

Name: aspA [H]

Synonym: BCZK2845

Alternate gene names: 52142397

Gene position: 2963224-2961794 (Counterclockwise)

Preceding gene: 52142396

Following gene: 52142398

Centisome position: 55.9

GC content: 39.62

Gene sequence:

>1431_bases
ATGGCAATGCCAAACAAGAACGTGCGAGTGGAGAATGATTTTTTGGGAGGAAAGGAATTACCTATTGAGGCATATTACGG
AATTCAAACTTTACGCGCTGTTGAAAATTTCCCGATTACAGGATACAAAATCCATGAAGGTTTAATTAGAGCGTTCGCAG
TTGTAAAAAAAGCTGCAGCGCTTGCCAATACAGATGTAGGAAGATTGGAATTGAACAAGGGCGGCGCGATCGCAGAAGCT
GCTCAAGAAATTCTCGATGGAAAATGGCATGATCATTTTATCGTAGATCCAATCCAAGGCGGAGCAGGTACTTCAATGAA
CATGAATGCAAATGAAGTCATTGCCAATCGTGCTCTTGAATTATTAGGAATGGAAAAGGGAGACTATCACTATATTAGTC
CAAATAGTCATGTAAACATGGCGCAATCAACAAACGATGCATTCCCAACGGCGATTCATATCGCTACATTAAACGCATTA
GAAGGTTTATTACAAACGATGGGTTATATGCACGATGTATTTGAATTAAAAGCAGAACAGTTCGATCATGTGATTAAAAT
GGGTCGTACACATTTACAAGACGCTGTGCCAATTCGACTTGGACAAGAATTTAAAGCGTACTCTCGTGTACTTGAACGTG
ATATGAAACGAATTCAGCAATCACGTCAACACTTATATGAAGTGAACATGGGAGCAACTGCAGTTGGTACAGGCTTAAAT
GCAGATCCAGAATATATTGAAGCGGTTGTAAAACATTTAGCTGCAATTAGTGAACTGCCACTTGTCGGTGCAGAAGACTT
AGTAGATGCAACGCAAAATACAGATGCATACACAGAAGTATCAGCAGCACTTAAAGTATGTATGATGAATATGTCTAAAA
TTGCGAATGACCTTCGCTTAATGGCATCAGGTCCACGTGTTGGCTTAGCGGAAATTATGCTACCTGCTCGTCAACCAGGT
TCATCTATTATGCCAGGGAAAGTAAACCCTGTTATGCCAGAGGTAATTAATCAAATTGCGTTCCAAGTAATTGGTAACGA
TCATACAATTTGCCTTGCTTCAGAAGCAGGTCAATTAGAATTAAACGTTATGGAACCAGTACTTGTTTTCAACTTACTTC
AATCAATTAGCATTATGAATAACGGTTTCCGTGCCTTTACAGATAATTGCTTAAAAGGAATTGAAGCGAATGAAGATCGC
TTAAAAGAGTATGTTGAGAAGAGTGTAGGAATTATTACAGCCGTGAACCCTCATATCGGTTATGAAGCAGCAGCTCGCGT
TGCGAAAGAAGCAATCGCGACAGGACAATCCGTTCGAGAACTTTGTGTGAGAAATGGTATATTGTCACAAGAAGACTTAG
AGTTAATTCTAGATCCGTTCGAAATGACACACCCTGGGATTGCAGGAGCAACTCTTTTAAATAAAAATTAA

Upstream 100 bases:

>100_bases
AGCCTTTAGCCTACGATTTAACAATAAAATCTGATAAGGATTGGTAAGGACTTCATATGATTTATTTAGGTTTTACATTT
AATATGGGGGAGAAATTAAA

Downstream 100 bases:

>100_bases
AAGAAGAGGGGGATTAATCTGGCACCTGTAGTTAGGACACTTGAAAAAAAGTGTTTTACCTACAGGTATTTTTGTATACT
TGGATTTTAAGATATAAAGA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAMPNKNVRVENDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHEGLIRAFAVVKKAAALANTDVGRLELNKGGAIAEA
AQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNAL
EGLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLN
ADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPARQPG
SSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDR
LKEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVRNGILSQEDLELILDPFEMTHPGIAGATLLNKN

Sequences:

>Translated_476_residues
MAMPNKNVRVENDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHEGLIRAFAVVKKAAALANTDVGRLELNKGGAIAEA
AQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNAL
EGLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLN
ADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPARQPG
SSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDR
LKEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVRNGILSQEDLELILDPFEMTHPGIAGATLLNKN
>Mature_475_residues
AMPNKNVRVENDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHEGLIRAFAVVKKAAALANTDVGRLELNKGGAIAEAA
QEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNALE
GLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLNA
DPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPARQPGS
SIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDRL
KEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVRNGILSQEDLELILDPFEMTHPGIAGATLLNKN

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=42.4242424242424, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI68303547, Length=245, Percent_Identity=29.7959183673469, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI31541964, Length=244, Percent_Identity=29.9180327868852, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI68303542, Length=244, Percent_Identity=29.9180327868852, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI87082375, Length=462, Percent_Identity=49.7835497835498, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=41.9006479481641, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17553882, Length=466, Percent_Identity=43.3476394849785, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=42.2018348623853, Blast_Score=273, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6324993, Length=464, Percent_Identity=41.5948275862069, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640179, Length=469, Percent_Identity=41.7910447761194, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640177, Length=471, Percent_Identity=41.4012738853503, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=42.1166306695464, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=40.6926406926407, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24583245, Length=464, Percent_Identity=38.3620689655172, Blast_Score=338, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51952; Mature: 51821

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMPNKNVRVENDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHEGLIRAFAVVKKAAA
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LANTDVGRLELNKGGAIAEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALE
HHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
LLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNALEGLLQTMGYMHDVFELKAEQ
HHCCCCCCEEEECCCCCEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLN
HHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
ADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRL
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MASGPRVGLAEIMLPARQPGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLE
HCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEE
LNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDRLKEYVEKSVGIITAVNPHIG
EEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCC
YEAAARVAKEAIATGQSVRELCVRNGILSQEDLELILDPFEMTHPGIAGATLLNKN
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AMPNKNVRVENDFLGGKELPIEAYYGIQTLRAVENFPITGYKIHEGLIRAFAVVKKAAA
CCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LANTDVGRLELNKGGAIAEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALE
HHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
LLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNALEGLLQTMGYMHDVFELKAEQ
HHCCCCCCEEEECCCCCEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTGLN
HHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
ADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRL
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MASGPRVGLAEIMLPARQPGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLE
HCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEE
LNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDRLKEYVEKSVGIITAVNPHIG
EEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCC
YEAAARVAKEAIATGQSVRELCVRNGILSQEDLELILDPFEMTHPGIAGATLLNKN
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]