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The map label for this gene is aspG [H]

Identifier: 52142396

GI number: 52142396

Start: 2963278

End: 2964282

Strand: Reverse

Name: aspG [H]

Synonym: BCZK2846

Alternate gene names: 52142396

Gene position: 2964282-2963278 (Counterclockwise)

Preceding gene: 52142393

Following gene: 52142397

Centisome position: 55.92

GC content: 35.22

Gene sequence:

>1005_bases
ATGAAGAAAATTTTATTACTTGCAACTGGTGGGACAATTGCATCTGTGGAGGGGAATGAGGGGCTTGTTCCAGGATTGTC
CGCTGAGGAATTATTGAATTATTTTTCGAAGTCTTCTCGAAACTTAGAAATAGATTGCAAAATCTTAATGAATATTGATA
GTACCAATATGCAACCTGAGCACTGGAAAGAAATAGCTAATGCTGTTTTTAATCACTATGATGACTATGACGGGTTTGTG
ATTACACATGGAACGGATACGTTAGCTTATACATCTTCTGCGTTATCTTATATGCTGCAAGGTTTAAGAAAACCAGTTGT
TTTAACAGGTTCACAAGTACCTATTAGTTTTAAAAAGACAGACGCAAAGAAAAATGTAGCTGATGCTCTTAGGTTTGCCT
GTGAGAATGTTGGCGGTGTGTTTATTGTTTTTGATAGTAGGGTGATTATTGGGACGAGAGCAGTTAAAATGCGAACAAAA
AGTTATGATGCATTTGAAAGTGTGAATTCTCCATATGTTGCTGAGGTAAATGAAGATGAAGTGAAATATCATTGGAAGCC
AAAGTCCTCCCATAATGAACTTTCAATAGATACTAATTTATGTACGGATGTTTTTCTTATGAAATTGTATCCAGGTACAA
AGCTAGAGATATTTGATTGTTTAAAAGATTTGTATAAAGGGATTGTTATTGAAAGTTTTGGAAACGGCGGATTACCTTTT
GAAGGAAGGAACTTGCTTTCGAAAATTCAAGAGTTGACTGAAATGGGGATTGCTGTAGTGATTACTACTCAATGCCTGGA
AGAAGGAGAAGACATACTTCTCTATGAAGTAGGACGGAAAGTGGCACAACATCAAGTAATATTGTCTGGAGATATGAATA
CGGAAGCCATCATAGCAAAATTGATGTGGGTGCTTGGAAAAACTAATAAATTAGAAGAGATTAAAAAAATTATTGAAGAG
CCTTTAGCCTACGATTTAACAATAAAATCTGATAAGGATTGGTAA

Upstream 100 bases:

>100_bases
TACAAAATCTTGATTAATTCAAATTAAGTTCTATAATGATAAATATAAATACGCATTACGCGAACGATATATCGTTGTTT
ATTTTATGGAGGGGTTGTGT

Downstream 100 bases:

>100_bases
GGACTTCATATGATTTATTTAGGTTTTACATTTAATATGGGGGAGAAATTAAAATGGCAATGCCAAACAAGAACGTGCGA
GTGGAGAATGATTTTTTGGG

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase 1; L-asparagine amidohydrolase 1 [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPEHWKEIANAVFNHYDDYDGFV
ITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACENVGGVFIVFDSRVIIGTRAVKMRTK
SYDAFESVNSPYVAEVNEDEVKYHWKPKSSHNELSIDTNLCTDVFLMKLYPGTKLEIFDCLKDLYKGIVIESFGNGGLPF
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWVLGKTNKLEEIKKIIEE
PLAYDLTIKSDKDW

Sequences:

>Translated_334_residues
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPEHWKEIANAVFNHYDDYDGFV
ITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACENVGGVFIVFDSRVIIGTRAVKMRTK
SYDAFESVNSPYVAEVNEDEVKYHWKPKSSHNELSIDTNLCTDVFLMKLYPGTKLEIFDCLKDLYKGIVIESFGNGGLPF
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWVLGKTNKLEEIKKIIEE
PLAYDLTIKSDKDW
>Mature_334_residues
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPEHWKEIANAVFNHYDDYDGFV
ITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACENVGGVFIVFDSRVIIGTRAVKMRTK
SYDAFESVNSPYVAEVNEDEVKYHWKPKSSHNELSIDTNLCTDVFLMKLYPGTKLEIFDCLKDLYKGIVIESFGNGGLPF
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWVLGKTNKLEEIKKIIEE
PLAYDLTIKSDKDW

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=360, Percent_Identity=31.9444444444444, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1788065, Length=336, Percent_Identity=34.2261904761905, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1789327, Length=300, Percent_Identity=25.6666666666667, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17558278, Length=361, Percent_Identity=31.0249307479224, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI193207372, Length=361, Percent_Identity=31.0249307479224, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17558280, Length=361, Percent_Identity=31.0249307479224, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI193207374, Length=361, Percent_Identity=31.0249307479224, Blast_Score=164, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6320527, Length=331, Percent_Identity=25.6797583081571, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323189, Length=279, Percent_Identity=27.5985663082437, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323187, Length=279, Percent_Identity=27.5985663082437, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323186, Length=279, Percent_Identity=27.5985663082437, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323184, Length=279, Percent_Identity=27.5985663082437, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24639609, Length=282, Percent_Identity=35.4609929078014, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24645530, Length=266, Percent_Identity=36.4661654135338, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37325; Mature: 37325

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPE
CCEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHH
HWKEIANAVFNHYDDYDGFVITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKT
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECC
DAKKNVADALRFACENVGGVFIVFDSRVIIGTRAVKMRTKSYDAFESVNSPYVAEVNEDE
CCHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEEEECCCCHHHHHCCCCEEEECCCCC
VKYHWKPKSSHNELSIDTNLCTDVFLMKLYPGTKLEIFDCLKDLYKGIVIESFGNGGLPF
EEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEECCCCCCCC
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAK
CCHHHHHHHHHHHHCCEEEEEEEHHHHCCCCEEEEHHHHHHHHCEEEEECCCCHHHHHHH
LMWVLGKTNKLEEIKKIIEEPLAYDLTIKSDKDW
HHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCC
>Mature Secondary Structure
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPE
CCEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHH
HWKEIANAVFNHYDDYDGFVITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKT
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECC
DAKKNVADALRFACENVGGVFIVFDSRVIIGTRAVKMRTKSYDAFESVNSPYVAEVNEDE
CCHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEEEECCCCHHHHHCCCCEEEECCCCC
VKYHWKPKSSHNELSIDTNLCTDVFLMKLYPGTKLEIFDCLKDLYKGIVIESFGNGGLPF
EEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEECCCCCCCC
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAK
CCHHHHHHHHHHHHCCEEEEEEEHHHHCCCCEEEEHHHHHHHHCEEEEECCCCHHHHHHH
LMWVLGKTNKLEEIKKIIEEPLAYDLTIKSDKDW
HHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377; 8478318 [H]