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The map label for this gene is hisS [H]

Identifier: 52142218

GI number: 52142218

Start: 3157145

End: 3158422

Strand: Direct

Name: hisS [H]

Synonym: BCZK3024

Alternate gene names: 52142218

Gene position: 3157145-3158422 (Clockwise)

Preceding gene: 52142219

Following gene: 52142217

Centisome position: 59.56

GC content: 37.64

Gene sequence:

>1278_bases
ATGGAAATGAAAAATGTAAAAGGAACGAAAGACTATTTACCAGAGGAACAAGTGCTGCGAAATAAAATAAAAAGAGCGTG
CGAAGACACGTTTGAACGATACGGATGTAAATCGTTAGAAACACCAACGTTAAATATGTATGAACTTATGTCTTACAAGT
ACGGCGGTGGTGATGAAATATTAAAAGAAATATATACGCTCAGGGATCAAGGAAAACGCGAACTTGCCTTACGTTACGAT
TTAACCATTCCATTCGCAAAAGTCGTGGCGATGAATCCGAACATCCGCCTCCCTTTTAAACGGTATGAAATTGGGAAAGT
ATTTCGAGATGGGCCCATTAAACAAGGAAGATTTCGTGAGTTCATTCAATGTGACGTTGATATAGTCGGTGTAGAATCTG
TCATGGCAGAAGCGGAACTTATGAGTATGGCGTTTGAACTGTTCCGAACGTTAAACTTAGAAGTAACGATCCAATATAAT
AACCGAAAATTGTTAAACGGTATTCTCGAGTCAATAAACATTCCTACTGAACGAACGAGTGACGTCATTTTATCATTAGA
CAAAATCGAAAAAATTGGGATTGATGGTGTACGAAAAGATGTACTTGAGCGCGGAATTTCTGAAGAAATGGCTGATACGA
TATGTAATACCGTTTTATCTTGTCTAAAGCTTTCGATTGATGACTTTAAAGACGCTTTCAATACTCCACTCGTTGCCGAA
GGAGTAAACGAACTACAACAATTACAGCAATATTTAATTGCTCTCGGAATAAATGAAAATGCTATATTCAATCCGTTTTT
AGCCCGAGGACTCACAATGTATACAGGCACCGTATATGAAATCTTTTTAAAAGATGGCTCGATTACATCTAGCATCGGTA
GCGGAGGGCGATACGATAATATTATTGGGGCATTCCGCGGTGATAATATGAACTATCCAACAGTCGGTATTTCATTCGGT
TTAGACGTTATTTATACAGCACTATCTCAGAAAGAAACAATATCATCTACAGCAGATATATTTATCATCCCAATCGGTAC
AGAGTTACAATGCTTACAAATTGCCCAGCAATTACGTTCTACCACTTCCTTAAAAGTCGAACTTGAACTAACAGGACGCA
AATTAAAACGTGCCCTTAATTATGCCAATAAAGAAAACATCCCTTATGTGCTTATTATTGGGGAAGAAGAACTTAGTACA
GAAACCGCTATGCTGCGGAATATGAAGGAAGGTAGTGAGGTGAAAGTTCCGCTCTCTTCTTTAAGTAATTATTTATAA

Upstream 100 bases:

>100_bases
GTACAGGGCTTTTCCTGTTGACCCACGAAGCGTTCATAAAGCTATGTGGGTATTTTTATACCTGCATAGCTTTTTTTCAT
TTAAAAAAGGAGTGATTATG

Downstream 100 bases:

>100_bases
TACAAACCACCCTCGTTTGGGTGGTTTTTCTATTTTATAGATACCTAACAATTGGTTTACATAAAATTATTATAATCTAT
TAAAAGCTACAAATAAACAA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase 1; HisRS 1 [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MEMKNVKGTKDYLPEEQVLRNKIKRACEDTFERYGCKSLETPTLNMYELMSYKYGGGDEILKEIYTLRDQGKRELALRYD
LTIPFAKVVAMNPNIRLPFKRYEIGKVFRDGPIKQGRFREFIQCDVDIVGVESVMAEAELMSMAFELFRTLNLEVTIQYN
NRKLLNGILESINIPTERTSDVILSLDKIEKIGIDGVRKDVLERGISEEMADTICNTVLSCLKLSIDDFKDAFNTPLVAE
GVNELQQLQQYLIALGINENAIFNPFLARGLTMYTGTVYEIFLKDGSITSSIGSGGRYDNIIGAFRGDNMNYPTVGISFG
LDVIYTALSQKETISSTADIFIIPIGTELQCLQIAQQLRSTTSLKVELELTGRKLKRALNYANKENIPYVLIIGEEELST
ETAMLRNMKEGSEVKVPLSSLSNYL

Sequences:

>Translated_425_residues
MEMKNVKGTKDYLPEEQVLRNKIKRACEDTFERYGCKSLETPTLNMYELMSYKYGGGDEILKEIYTLRDQGKRELALRYD
LTIPFAKVVAMNPNIRLPFKRYEIGKVFRDGPIKQGRFREFIQCDVDIVGVESVMAEAELMSMAFELFRTLNLEVTIQYN
NRKLLNGILESINIPTERTSDVILSLDKIEKIGIDGVRKDVLERGISEEMADTICNTVLSCLKLSIDDFKDAFNTPLVAE
GVNELQQLQQYLIALGINENAIFNPFLARGLTMYTGTVYEIFLKDGSITSSIGSGGRYDNIIGAFRGDNMNYPTVGISFG
LDVIYTALSQKETISSTADIFIIPIGTELQCLQIAQQLRSTTSLKVELELTGRKLKRALNYANKENIPYVLIIGEEELST
ETAMLRNMKEGSEVKVPLSSLSNYL
>Mature_425_residues
MEMKNVKGTKDYLPEEQVLRNKIKRACEDTFERYGCKSLETPTLNMYELMSYKYGGGDEILKEIYTLRDQGKRELALRYD
LTIPFAKVVAMNPNIRLPFKRYEIGKVFRDGPIKQGRFREFIQCDVDIVGVESVMAEAELMSMAFELFRTLNLEVTIQYN
NRKLLNGILESINIPTERTSDVILSLDKIEKIGIDGVRKDVLERGISEEMADTICNTVLSCLKLSIDDFKDAFNTPLVAE
GVNELQQLQQYLIALGINENAIFNPFLARGLTMYTGTVYEIFLKDGSITSSIGSGGRYDNIIGAFRGDNMNYPTVGISFG
LDVIYTALSQKETISSTADIFIIPIGTELQCLQIAQQLRSTTSLKVELELTGRKLKRALNYANKENIPYVLIIGEEELST
ETAMLRNMKEGSEVKVPLSSLSNYL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=440, Percent_Identity=32.9545454545455, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI6996014, Length=443, Percent_Identity=32.5056433408578, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI65287717, Length=428, Percent_Identity=26.8691588785047, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788861, Length=456, Percent_Identity=22.3684210526316, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71993686, Length=456, Percent_Identity=31.140350877193, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI71993693, Length=456, Percent_Identity=31.140350877193, Blast_Score=197, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6325290, Length=387, Percent_Identity=33.5917312661499, Blast_Score=187, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24643061, Length=462, Percent_Identity=31.3852813852814, Blast_Score=208, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24643059, Length=481, Percent_Identity=30.1455301455301, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47971; Mature: 47971

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMKNVKGTKDYLPEEQVLRNKIKRACEDTFERYGCKSLETPTLNMYELMSYKYGGGDEI
CCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHH
LKEIYTLRDQGKRELALRYDLTIPFAKVVAMNPNIRLPFKRYEIGKVFRDGPIKQGRFRE
HHHHHHHHHCCCCEEEEEEECCCCHHHEEEECCCEECCHHHHHHHHHHCCCCCCCHHHHH
FIQCDVDIVGVESVMAEAELMSMAFELFRTLNLEVTIQYNNRKLLNGILESINIPTERTS
HHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHCCCCCCCCH
DVILSLDKIEKIGIDGVRKDVLERGISEEMADTICNTVLSCLKLSIDDFKDAFNTPLVAE
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
GVNELQQLQQYLIALGINENAIFNPFLARGLTMYTGTVYEIFLKDGSITSSIGSGGRYDN
HHHHHHHHHHHHHHHCCCCCCEECHHHHCCHHHHCCEEEEEEEECCCEECCCCCCCCCCC
IIGAFRGDNMNYPTVGISFGLDVIYTALSQKETISSTADIFIIPIGTELQCLQIAQQLRS
CEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHC
TTSLKVELELTGRKLKRALNYANKENIPYVLIIGEEELSTETAMLRNMKEGSEVKVPLSS
CCEEEEEEEECHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEHHH
LSNYL
HCCCC
>Mature Secondary Structure
MEMKNVKGTKDYLPEEQVLRNKIKRACEDTFERYGCKSLETPTLNMYELMSYKYGGGDEI
CCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCHHHH
LKEIYTLRDQGKRELALRYDLTIPFAKVVAMNPNIRLPFKRYEIGKVFRDGPIKQGRFRE
HHHHHHHHHCCCCEEEEEEECCCCHHHEEEECCCEECCHHHHHHHHHHCCCCCCCHHHHH
FIQCDVDIVGVESVMAEAELMSMAFELFRTLNLEVTIQYNNRKLLNGILESINIPTERTS
HHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHCCCCCCCCH
DVILSLDKIEKIGIDGVRKDVLERGISEEMADTICNTVLSCLKLSIDDFKDAFNTPLVAE
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
GVNELQQLQQYLIALGINENAIFNPFLARGLTMYTGTVYEIFLKDGSITSSIGSGGRYDN
HHHHHHHHHHHHHHHCCCCCCEECHHHHCCHHHHCCEEEEEEEECCCEECCCCCCCCCCC
IIGAFRGDNMNYPTVGISFGLDVIYTALSQKETISSTADIFIIPIGTELQCLQIAQQLRS
CEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHC
TTSLKVELELTGRKLKRALNYANKENIPYVLIIGEEELSTETAMLRNMKEGSEVKVPLSS
CCEEEEEEEECHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEHHH
LSNYL
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA