Click here to switch to the map view.

The map label for this gene is yfiK [H]

Identifier: 52142219

GI number: 52142219

Start: 3155070

End: 3156863

Strand: Direct

Name: yfiK [H]

Synonym: BCZK3023

Alternate gene names: 52142219

Gene position: 3155070-3156863 (Clockwise)

Preceding gene: 52142225

Following gene: 52142218

Centisome position: 59.52

GC content: 35.51

Gene sequence:

>1794_bases
ATGTTCTCATTCACAAAAGAATGGATTTGGTATGACTGGATATTTGTGCTCATACGAACATTTTGGCTAATTGTTATTAT
CAGCGCTAATTTTATGTTCCCAACATCCATTCATACATCAAGTATGATTGTACTTTCTCTCACTTTTGTCGTTTATCTTG
TACCATTAATTATTCGATATAAGAAACCAGGATGGTACCCTGCATTCGATATTATCACTGCGGGTTGTTTTTATCTTTAC
TTAGCATCCGTAGCTCCAAATTTACTTTGGTCTTTCATTTTAGTCGTAATCATTATTGGACTATGTAGTAATCGAAATAA
TTATATATGGAGCAGTATTTTTTGTGGATTTGCATTTCCGCTATTGAATGCTTGGATTGCCAACCGATCGCCATATGAAC
TAATCGTTAGTTGTAGTCTCGGTTTTGCCATTGGCATTTCTTTTAATATATTAATTCAGTATCATAAGCAATCTCGAATT
ATTGAAGAGCAAAAACTACTATTAGAGCAACATATTAAAAGAATTGAAGAACTTACTTTAATAGAAGAGCGTAATCGACT
GTCACATGAGCTACATGACACAATTGGTCATACACTTACTTCTCTCATCGCTGGTGTCACATCACTAAGATCATCAGTAC
CGGATTCGCAGTTTGAACGAATTGATTCCCTTATCAGCATTGCTCAGCATAGTCTAAATGATATTCGAAAGCATCTACAT
GAGCTATCTCATAATCCACTTAGTAATTCATTAAGTGAATCCTTGCAACAATTAACAGATGAATTTATGAAATCTACAGG
TACAACCGTTACATTTCGTATGATTGGAAACGAAACCCTTGTAATGCAAAAAATAAATTTTTGCCTATACCGTTGTCTTC
AAGAGTCGTTAACAAATGCTGTTCGACACGGTAAAGCGAGCAACATATCCGTTCAACTACATTTCGACCACCAACAACTT
CGATTGCAAATTGAAGATAACGGGATTGGAATGAAAGAAATTCAATTTGGCTTTGGACTCAGTGGAATGAAAGAGCGACT
TGAACAAGTTCAGGGGACATTATCGGTTCAGTCTAATGTTGAACAGGGAACATTAATTGTTTGTAATATTCCATTACAAA
CAAAACCTGTACATGACATCATTCGCTTATTGGTCGTTGATGATCAGGCACTTATTACAAATAGTTTAGAGCAAATTTTA
GAGAACCAAACTGATTTTATTGTTGCTGGGAAGGCATACGATGGATCTGAGGCATTAATATTATGTGAACAATTAGAACC
TGACATTGTACTAATGGACATTCAGATGCCAGAAATGAATGGTATTGAGGCTTTACTAGAAATGAAGCGACGTTGGCCTA
ATATGAAGATTGTACTTATGACAACATTTGAAGATTCCTTACAAGCAGCAACTGCACTCGAGCATGGAGCTGAAGGTTAC
ATGTTGAAATCTATTCATCCACAAGAAATGAAGGAGGCCTTGAAACTTATTTATAACGGTGGAACTTGGATTGACCAATC
AGTTGCTACACGTATTTTTGAGGAAATGAAACTTCAACGTGAGCAATTAGCGAAAATCAAATCAACTAAACAAACTTTTC
CATACGGACTTACAAAACGGGAAATGGAAATTTTAGAACATCTATCCAACGGATTACGCTATAAATCTATTGCAGCTAAA
CTATTTTTATCTGAAGGAACAATCCGTAATTACTGCTCAAATCTCTATTCAAAGCTAGGTGTTAGCAATCGAGAAGAAGC
GATAAAAATGGCACGAACAGAAAATATCTTGTAG

Upstream 100 bases:

>100_bases
GTTTGTTGACTGTTTTTCTATTGACGATTTCAAAGATATGTTACATGATGAATCTGAATCATTAGTAATTAATCTTAAAA
TACAAGTAGGTGCTTAATTT

Downstream 100 bases:

>100_bases
GCAATGTGAAATGAATACATTTCGCTTCAAAACTGAAAATATTGATAATTCAAAAATCATCTGATATAATTTGGAAAAAC
TTATGTTAAGGAGAGATTTG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MFSFTKEWIWYDWIFVLIRTFWLIVIISANFMFPTSIHTSSMIVLSLTFVVYLVPLIIRYKKPGWYPAFDIITAGCFYLY
LASVAPNLLWSFILVVIIIGLCSNRNNYIWSSIFCGFAFPLLNAWIANRSPYELIVSCSLGFAIGISFNILIQYHKQSRI
IEEQKLLLEQHIKRIEELTLIEERNRLSHELHDTIGHTLTSLIAGVTSLRSSVPDSQFERIDSLISIAQHSLNDIRKHLH
ELSHNPLSNSLSESLQQLTDEFMKSTGTTVTFRMIGNETLVMQKINFCLYRCLQESLTNAVRHGKASNISVQLHFDHQQL
RLQIEDNGIGMKEIQFGFGLSGMKERLEQVQGTLSVQSNVEQGTLIVCNIPLQTKPVHDIIRLLVVDDQALITNSLEQIL
ENQTDFIVAGKAYDGSEALILCEQLEPDIVLMDIQMPEMNGIEALLEMKRRWPNMKIVLMTTFEDSLQAATALEHGAEGY
MLKSIHPQEMKEALKLIYNGGTWIDQSVATRIFEEMKLQREQLAKIKSTKQTFPYGLTKREMEILEHLSNGLRYKSIAAK
LFLSEGTIRNYCSNLYSKLGVSNREEAIKMARTENIL

Sequences:

>Translated_597_residues
MFSFTKEWIWYDWIFVLIRTFWLIVIISANFMFPTSIHTSSMIVLSLTFVVYLVPLIIRYKKPGWYPAFDIITAGCFYLY
LASVAPNLLWSFILVVIIIGLCSNRNNYIWSSIFCGFAFPLLNAWIANRSPYELIVSCSLGFAIGISFNILIQYHKQSRI
IEEQKLLLEQHIKRIEELTLIEERNRLSHELHDTIGHTLTSLIAGVTSLRSSVPDSQFERIDSLISIAQHSLNDIRKHLH
ELSHNPLSNSLSESLQQLTDEFMKSTGTTVTFRMIGNETLVMQKINFCLYRCLQESLTNAVRHGKASNISVQLHFDHQQL
RLQIEDNGIGMKEIQFGFGLSGMKERLEQVQGTLSVQSNVEQGTLIVCNIPLQTKPVHDIIRLLVVDDQALITNSLEQIL
ENQTDFIVAGKAYDGSEALILCEQLEPDIVLMDIQMPEMNGIEALLEMKRRWPNMKIVLMTTFEDSLQAATALEHGAEGY
MLKSIHPQEMKEALKLIYNGGTWIDQSVATRIFEEMKLQREQLAKIKSTKQTFPYGLTKREMEILEHLSNGLRYKSIAAK
LFLSEGTIRNYCSNLYSKLGVSNREEAIKMARTENIL
>Mature_597_residues
MFSFTKEWIWYDWIFVLIRTFWLIVIISANFMFPTSIHTSSMIVLSLTFVVYLVPLIIRYKKPGWYPAFDIITAGCFYLY
LASVAPNLLWSFILVVIIIGLCSNRNNYIWSSIFCGFAFPLLNAWIANRSPYELIVSCSLGFAIGISFNILIQYHKQSRI
IEEQKLLLEQHIKRIEELTLIEERNRLSHELHDTIGHTLTSLIAGVTSLRSSVPDSQFERIDSLISIAQHSLNDIRKHLH
ELSHNPLSNSLSESLQQLTDEFMKSTGTTVTFRMIGNETLVMQKINFCLYRCLQESLTNAVRHGKASNISVQLHFDHQQL
RLQIEDNGIGMKEIQFGFGLSGMKERLEQVQGTLSVQSNVEQGTLIVCNIPLQTKPVHDIIRLLVVDDQALITNSLEQIL
ENQTDFIVAGKAYDGSEALILCEQLEPDIVLMDIQMPEMNGIEALLEMKRRWPNMKIVLMTTFEDSLQAATALEHGAEGY
MLKSIHPQEMKEALKLIYNGGTWIDQSVATRIFEEMKLQREQLAKIKSTKQTFPYGLTKREMEILEHLSNGLRYKSIAAK
LFLSEGTIRNYCSNLYSKLGVSNREEAIKMARTENIL

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=213, Percent_Identity=30.0469483568075, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788521, Length=204, Percent_Identity=29.9019607843137, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1790102, Length=207, Percent_Identity=28.5024154589372, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1787473, Length=205, Percent_Identity=28.2926829268293, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1786747, Length=214, Percent_Identity=26.6355140186916, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788812, Length=214, Percent_Identity=29.9065420560748, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787474, Length=233, Percent_Identity=25.7510729613734, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788712, Length=196, Percent_Identity=23.9795918367347, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87082311, Length=218, Percent_Identity=27.9816513761468, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788550, Length=115, Percent_Identity=37.3913043478261, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082052, Length=117, Percent_Identity=31.6239316239316, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 68329; Mature: 68329

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSFTKEWIWYDWIFVLIRTFWLIVIISANFMFPTSIHTSSMIVLSLTFVVYLVPLIIRY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
KKPGWYPAFDIITAGCFYLYLASVAPNLLWSFILVVIIIGLCSNRNNYIWSSIFCGFAFP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LLNAWIANRSPYELIVSCSLGFAIGISFNILIQYHKQSRIIEEQKLLLEQHIKRIEELTL
HHHHHHCCCCCCEEEEEECCCHHCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
IEERNRLSHELHDTIGHTLTSLIAGVTSLRSSVPDSQFERIDSLISIAQHSLNDIRKHLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELSHNPLSNSLSESLQQLTDEFMKSTGTTVTFRMIGNETLVMQKINFCLYRCLQESLTNA
HHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
VRHGKASNISVQLHFDHQQLRLQIEDNGIGMKEIQFGFGLSGMKERLEQVQGTLSVQSNV
HHCCCCCCEEEEEEECHHHEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
EQGTLIVCNIPLQTKPVHDIIRLLVVDDQALITNSLEQILENQTDFIVAGKAYDGSEALI
CCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEE
LCEQLEPDIVLMDIQMPEMNGIEALLEMKRRWPNMKIVLMTTFEDSLQAATALEHGAEGY
EEECCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCC
MLKSIHPQEMKEALKLIYNGGTWIDQSVATRIFEEMKLQREQLAKIKSTKQTFPYGLTKR
EECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EMEILEHLSNGLRYKSIAAKLFLSEGTIRNYCSNLYSKLGVSNREEAIKMARTENIL
HHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MFSFTKEWIWYDWIFVLIRTFWLIVIISANFMFPTSIHTSSMIVLSLTFVVYLVPLIIRY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
KKPGWYPAFDIITAGCFYLYLASVAPNLLWSFILVVIIIGLCSNRNNYIWSSIFCGFAFP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LLNAWIANRSPYELIVSCSLGFAIGISFNILIQYHKQSRIIEEQKLLLEQHIKRIEELTL
HHHHHHCCCCCCEEEEEECCCHHCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
IEERNRLSHELHDTIGHTLTSLIAGVTSLRSSVPDSQFERIDSLISIAQHSLNDIRKHLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELSHNPLSNSLSESLQQLTDEFMKSTGTTVTFRMIGNETLVMQKINFCLYRCLQESLTNA
HHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
VRHGKASNISVQLHFDHQQLRLQIEDNGIGMKEIQFGFGLSGMKERLEQVQGTLSVQSNV
HHCCCCCCEEEEEEECHHHEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
EQGTLIVCNIPLQTKPVHDIIRLLVVDDQALITNSLEQILENQTDFIVAGKAYDGSEALI
CCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEE
LCEQLEPDIVLMDIQMPEMNGIEALLEMKRRWPNMKIVLMTTFEDSLQAATALEHGAEGY
EEECCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCC
MLKSIHPQEMKEALKLIYNGGTWIDQSVATRIFEEMKLQREQLAKIKSTKQTFPYGLTKR
EECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EMEILEHLSNGLRYKSIAAKLFLSEGTIRNYCSNLYSKLGVSNREEAIKMARTENIL
HHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]