BacMap

Definition	Bacillus cereus E33L, complete genome.
Accession	NC_006274
Length	5,300,915

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The map label for this gene is ytbE [H]

Identifier: 52142140

GI number: 52142140

Start: 3233289

End: 3234128

Strand: Reverse

Name: ytbE [H]

Synonym: BCZK3101

Alternate gene names: 52142140

Gene position: 3234128-3233289 (Counterclockwise)

Preceding gene: 52142141

Following gene: 52142143

Centisome position: 61.01

GC content: 35.6

Gene sequence:

>840_bases
ATGAAAAATTTACAAAGTACAACAACATTGCATAACGGTGTAGAAATGCCTTGGTTCGGTTTAGGCGTATTTAAAGTAGA
AGAAGGACCAGAACTTGTAGAAGCAATTAAATCAGCAATTAAGGCAGGATACCGTAGCATCGATACAGCTGCGATTTATG
GAAATGAAGCAGCTGTAGGTGAAGGAATTCGCGCTGGTATTGAAGCGACTGGCATTTCAAGAGAAGAATTATTTATCACT
TCAAAAGTATGGAACGCAGATCAAGGATACGAAGAAACAATTGCTGCATATGAAGAGAGTTTAAAAAAATTACAACTAGA
TTATTTAGATTTATATCTTGTTCACTGGCCTGTAGAAGGGAAATATAAAGATACGTGGAGAGCGTTAGAAACGCTTTATA
AAGAAAAGCGTGTACGTGCAATTGGCGTAAGTAACTTCCAAATTCATCACCTGCAAGATGTAATAAAAGATGCAGAAATT
AAGCCAATGATTAACCAAGTAGAATACCACCCTCGTTTGACACAAAAAGAATTACAAGCTTTCTGTAAAGAACAAGGTAT
TCAAATGGAAGCATGGTCACCATTAATGCAAGGTCAATTATTAGATAACGAAACATTACAAGAGATTGCTGAGAAACATG
GTAAAACGACAGCGCAAGTTATTTTACGTTGGGATCTTCAAAATGGAGTTATTACAATTCCAAAATCAACGAAAGAACAA
CGTATTATTGCAAACGCTGATGTATTTAACTTTGAATTAACGAAAGAAGATATGGAAAAAATCGATGCGTTAAATCAAAA
CCACCGCGTTGGTCCAGATCCTGATAACTTTGATTTCTAA

Upstream 100 bases:

>100_bases
TGGGTAGGCGGCATTATGGTAGTAGGTGCTGTTATTTTAACAGCGTGGAGTATGAGATTAGAAAAACGAGATCAAGTAAA
ATAAAAGGAGAGAAAATAAA

Downstream 100 bases:

>100_bases
GATTAAAGAATGAGTAAAAAGAGTGCAAAGTTGCACTCTTTTTTATTTTCTATTCAATAAAATGTTTCTGTTTATTTGAT
TAGCGTTTATATTATGGCTG

Product: aldo/keto reductase family oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKNLQSTTTLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKAGYRSIDTAAIYGNEAAVGEGIRAGIEATGISREELFIT
SKVWNADQGYEETIAAYEESLKKLQLDYLDLYLVHWPVEGKYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIKDAEI
KPMINQVEYHPRLTQKELQAFCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEQ
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF

Sequences:

>Translated_279_residues
MKNLQSTTTLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKAGYRSIDTAAIYGNEAAVGEGIRAGIEATGISREELFIT
SKVWNADQGYEETIAAYEESLKKLQLDYLDLYLVHWPVEGKYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIKDAEI
KPMINQVEYHPRLTQKELQAFCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEQ
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF
>Mature_279_residues
MKNLQSTTTLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKAGYRSIDTAAIYGNEAAVGEGIRAGIEATGISREELFIT
SKVWNADQGYEETIAAYEESLKKLQLDYLDLYLVHWPVEGKYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIKDAEI
KPMINQVEYHPRLTQKELQAFCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEQ
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=295, Percent_Identity=40, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI5174391, Length=295, Percent_Identity=40, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI223468663, Length=298, Percent_Identity=39.261744966443, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI310109922, Length=300, Percent_Identity=39.3333333333333, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI310109920, Length=320, Percent_Identity=38.4375, Blast_Score=197, Evalue=6e-51,
Organism=Homo sapiens, GI93277124, Length=305, Percent_Identity=37.0491803278689, Blast_Score=197, Evalue=8e-51,
Organism=Homo sapiens, GI45446745, Length=320, Percent_Identity=38.125, Blast_Score=197, Evalue=8e-51,
Organism=Homo sapiens, GI4503285, Length=320, Percent_Identity=38.125, Blast_Score=197, Evalue=8e-51,
Organism=Homo sapiens, GI5453543, Length=319, Percent_Identity=37.9310344827586, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI24497585, Length=295, Percent_Identity=39.3220338983051, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI291291012, Length=278, Percent_Identity=39.2086330935252, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI24497583, Length=302, Percent_Identity=37.0860927152318, Blast_Score=188, Evalue=4e-48,
Organism=Homo sapiens, GI4502049, Length=299, Percent_Identity=38.7959866220736, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=35.472972972973, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=34.1818181818182, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI300116271, Length=274, Percent_Identity=33.9416058394161, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI310109924, Length=160, Percent_Identity=39.375, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI310109926, Length=123, Percent_Identity=43.9024390243902, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI207028673, Length=123, Percent_Identity=43.0894308943089, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI87082198, Length=276, Percent_Identity=45.2898550724638, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1786400, Length=263, Percent_Identity=37.6425855513308, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1788081, Length=265, Percent_Identity=33.9622641509434, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1788070, Length=237, Percent_Identity=26.5822784810127, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI48994888, Length=292, Percent_Identity=25.6849315068493, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081735, Length=282, Percent_Identity=23.4042553191489, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787674, Length=274, Percent_Identity=25.1824817518248, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71998625, Length=273, Percent_Identity=41.7582417582418, Blast_Score=212, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17564128, Length=299, Percent_Identity=36.4548494983278, Blast_Score=204, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17537077, Length=295, Percent_Identity=40.6779661016949, Blast_Score=200, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17537075, Length=304, Percent_Identity=39.8026315789474, Blast_Score=198, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17561298, Length=271, Percent_Identity=36.1623616236162, Blast_Score=184, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17550248, Length=300, Percent_Identity=37, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537079, Length=312, Percent_Identity=33.6538461538462, Blast_Score=181, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17552492, Length=273, Percent_Identity=33.3333333333333, Blast_Score=180, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17538386, Length=271, Percent_Identity=36.90036900369, Blast_Score=177, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17562292, Length=274, Percent_Identity=32.4817518248175, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17561300, Length=271, Percent_Identity=34.3173431734317, Blast_Score=158, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17566692, Length=287, Percent_Identity=32.7526132404181, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321896, Length=317, Percent_Identity=36.5930599369085, Blast_Score=203, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6320576, Length=300, Percent_Identity=36.3333333333333, Blast_Score=193, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6324694, Length=290, Percent_Identity=36.551724137931, Blast_Score=182, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6322556, Length=260, Percent_Identity=35.7692307692308, Blast_Score=182, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6319625, Length=297, Percent_Identity=36.7003367003367, Blast_Score=178, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6320079, Length=291, Percent_Identity=35.0515463917526, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24663317, Length=298, Percent_Identity=39.9328859060403, Blast_Score=210, Evalue=7e-55,
Organism=Drosophila melanogaster, GI24644950, Length=300, Percent_Identity=38.6666666666667, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24662789, Length=294, Percent_Identity=38.7755102040816, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI221378297, Length=320, Percent_Identity=36.25, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI24657054, Length=291, Percent_Identity=38.4879725085911, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI20129731, Length=296, Percent_Identity=36.8243243243243, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24662785, Length=291, Percent_Identity=41.5807560137457, Blast_Score=177, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24662781, Length=293, Percent_Identity=41.296928327645, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI45553081, Length=295, Percent_Identity=32.8813559322034, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI21356425, Length=291, Percent_Identity=34.3642611683849, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI281366140, Length=256, Percent_Identity=32.8125, Blast_Score=153, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 31822; Mature: 31822

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLQSTTTLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKAGYRSIDTAAIYGNEAAVG
CCCCCCHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHC
EGIRAGIEATGISREELFITSKVWNADQGYEETIAAYEESLKKLQLDYLDLYLVHWPVEG
CHHHCCCHHCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCC
KYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIKDAEIKPMINQVEYHPRLTQKELQA
CHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHH
FCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEQ
HHHHCCCCCHHCCHHHCCCCCCHHHHHHHHHHHCCCEEHEEEEEECCCCEEECCCCCCCC
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF
EEEECCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKNLQSTTTLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKAGYRSIDTAAIYGNEAAVG
CCCCCCHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHC
EGIRAGIEATGISREELFITSKVWNADQGYEETIAAYEESLKKLQLDYLDLYLVHWPVEG
CHHHCCCHHCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCC
KYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIKDAEIKPMINQVEYHPRLTQKELQA
CHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHH
FCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEQ
HHHHCCCCCHHCCHHHCCCCCCHHHHHHHHHHHCCCEEHEEEEEECCCCEEECCCCCCCC
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF
EEEECCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]