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The map label for this gene is fadD [H]

Identifier: 52141914

GI number: 52141914

Start: 3463354

End: 3465033

Strand: Reverse

Name: fadD [H]

Synonym: BCZK3330

Alternate gene names: 52141914

Gene position: 3465033-3463354 (Counterclockwise)

Preceding gene: 52141912

Following gene: 52141915

Centisome position: 65.37

GC content: 34.64

Gene sequence:

>1680_bases
GTGGGAGACGATGAAAGTGAGTGCTGTGTTTTTACCAGGGGATTGCATAAAAGCTATTATTGCAGCGATTCTTGTAACGA
AATTACATCGTTCATTGAAACATATTATTACGCCTGCTCTAAAGAATGGGAAATATACAAATGCGGGATAAGAAAGAGAA
GAAACACGTTTATTGCGTGTTTCTTTTTAGTATTTTGTAAAGAAAGGACATCTCATATGGGAATAACAAAAGAATATAAA
AAACATGCCTCTTTACAACCGAATAAAATAGCAATAAAGGAAAATGATCGAGTTTTAACATATAAAGAGTGGTTTGAGTC
AGTGTATAAAGTAGCAAACTGGTTGAATGAAAAAGAATCGAAGAATAAAACGATAGCGATTGTATTAGAAAATCGTATAG
AGTTTTTACAACTATTCGCGGGTGCTGCTATGGCCGGATGGGTTTGTGTGCCACTAGATATAAAGTGGAAACAAGATGAG
CTCAAAGAAAGAATTGCAATTAGTAATCCGGATATGATTGTGACAGAGAGATATAAGTTAAATGATTTACCGGATGAAGA
AGGAAGAGTAATTGAAATTGATGAGTGGAAAAGGATGATTGAGAAATATCTTCCTACATATTTCCCTATAGAAAATGTAC
AAAATGCTCCTTTTTATATGGGATTCACATCAGGATCGACTGGAAAAGCAAAAGCGTTTTTACGTGCGCAACAATCGTGG
CTTCATAGTTTTGATTGTAATGTACATGATTTTCATATGAAAAGAGAGGATTCGATTCTAATAGCTGGGACGCTTGTTCA
TTCTCTTTTCTTATACGGGGCAATAAGCGCATTATATGTAGGACAAACGGTTCACATTATGAGAAAGTTCATTCCAAATC
AAGTATTAGATCAGTTAGAAACTGAAAATATATCAGTTATGTATACAGTTCCGACAATGCTTGAATCTCTATATAAAGAA
AATAGAGTGATAGAAAATAAAATGAAAATTATTTCGTCAGGGGCGAAATGGGAAGCTGGAGCGAAAGAAAAAATAAAGAA
TATATTTCCTTATGCGAAAAGATATGAATTTTATGGTGCATCGGAACTGAGTTTTGTAACAGCATTAGTTGATGAAGAGA
GCGAAAGAAGGCCAAATTCAGTAGGGAAACCTTGTCACAATGTGCAAGTTCGAGTATGTAATGAGGCAGGAAAAGAAGTA
CAGAAAGGTGAGATAGGAACTGTTTATGTGAAAAGTGATCAGTTTTTTATGGGATACATAATAGATGGTGTTTTAGCACG
GGAGTTGAATGCAGATGGCTGGATGACAGTGCGAGATGTAGGTTATGAAGATGAAGAAGGATTTATTTATATTATCGGTA
GAGAGAAGAATATGATTTTATTTGGAGGAATTAATATTTTCCCAGAAGAAATAGAAAGCGTATTACATGAACATCCAGCT
GTTGATGAAATAGTTGTAATTGGTGTGGAAGATAGTTACTGGGGTGAAAAACCTGTCGCGATTGTAAAAGGAAGTGCTAC
GAGGCAACAATTAAAGAGTTTTTGCTTACAAAGATTATCCTCTTTTAAAATACCGAAAGAATGGTATTTTGTAGATGAAA
TACCGTATACAAATAGCGGGAAAATCGCTCGTATGGAAGCGAAAAGTATCATTGAAAATCAGGAGAAAATATATGAATAG

Upstream 100 bases:

>100_bases
TTTCTATTATAAAAGTATTATGCATTAATATAATTGGTGGTATTTTCGTAGTTTATGCATTTGGTATTACTGTACAAGCT
TTCTTAATGGGTGTTTCCGT

Downstream 100 bases:

>100_bases
AGCAGTTATTGTAGAAGCGAAAAGGACACCTATTGGTAAGAAGAATGGGATGTTAAAAGACTATGAAGTTCAGCAATTAG
CAGCACCGCTTCTTACATTT

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MGDDESECCVFTRGLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIACFFLVFCKERTSHMGITKEYK
KHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKESKNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDE
LKERIAISNPDMIVTERYKLNDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENISVMYTVPTMLESLYKE
NRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEV
QKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPA
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKSFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIENQEKIYE

Sequences:

>Translated_559_residues
MGDDESECCVFTRGLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIACFFLVFCKERTSHMGITKEYK
KHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKESKNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDE
LKERIAISNPDMIVTERYKLNDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENISVMYTVPTMLESLYKE
NRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEV
QKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPA
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKSFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIENQEKIYE
>Mature_558_residues
GDDESECCVFTRGLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIACFFLVFCKERTSHMGITKEYKK
HASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKESKNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDEL
KERIAISNPDMIVTERYKLNDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSWL
HSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENISVMYTVPTMLESLYKEN
RVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEVQ
KGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPAV
DEIVVIGVEDSYWGEKPVAIVKGSATRQQLKSFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIENQEKIYE

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=356, Percent_Identity=27.5280898876405, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI187761345, Length=516, Percent_Identity=24.8062015503876, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI187761343, Length=516, Percent_Identity=24.8062015503876, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI157311624, Length=495, Percent_Identity=23.4343434343434, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI157311622, Length=495, Percent_Identity=23.4343434343434, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI58082049, Length=501, Percent_Identity=23.3532934131737, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI42544132, Length=284, Percent_Identity=27.8169014084507, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI38505220, Length=357, Percent_Identity=25.2100840336134, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI122937307, Length=371, Percent_Identity=25.6064690026954, Blast_Score=83, Evalue=9e-16,
Organism=Homo sapiens, GI115511026, Length=495, Percent_Identity=23.030303030303, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI13375727, Length=427, Percent_Identity=20.6088992974239, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI28416953, Length=558, Percent_Identity=23.8351254480287, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788107, Length=526, Percent_Identity=24.5247148288973, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI145693145, Length=503, Percent_Identity=24.0556660039761, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1786810, Length=510, Percent_Identity=24.1176470588235, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI221142682, Length=493, Percent_Identity=24.1379310344828, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788595, Length=337, Percent_Identity=25.8160237388724, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1790505, Length=399, Percent_Identity=22.5563909774436, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=534, Percent_Identity=24.7191011235955, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=486, Percent_Identity=25.9259259259259, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=527, Percent_Identity=24.0986717267552, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=301, Percent_Identity=30.8970099667774, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=365, Percent_Identity=28.2191780821918, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=349, Percent_Identity=26.647564469914, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=361, Percent_Identity=27.1468144044321, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=349, Percent_Identity=26.647564469914, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=349, Percent_Identity=26.647564469914, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=363, Percent_Identity=27.8236914600551, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=496, Percent_Identity=25, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=490, Percent_Identity=23.469387755102, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=495, Percent_Identity=24.040404040404, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI25141359, Length=486, Percent_Identity=21.3991769547325, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=532, Percent_Identity=25.187969924812, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=562, Percent_Identity=24.0213523131673, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24581924, Length=341, Percent_Identity=27.8592375366569, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI21355181, Length=486, Percent_Identity=24.8971193415638, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648260, Length=497, Percent_Identity=23.5412474849095, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI18859661, Length=498, Percent_Identity=24.4979919678715, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21356947, Length=229, Percent_Identity=26.6375545851528, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24653035, Length=200, Percent_Identity=28, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356441, Length=505, Percent_Identity=21.7821782178218, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24648253, Length=507, Percent_Identity=21.8934911242604, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648255, Length=199, Percent_Identity=29.6482412060301, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21358303, Length=136, Percent_Identity=33.8235294117647, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19922652, Length=356, Percent_Identity=23.314606741573, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24656500, Length=344, Percent_Identity=25.2906976744186, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI161076582, Length=205, Percent_Identity=27.8048780487805, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI20130357, Length=206, Percent_Identity=25.7281553398058, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281365686, Length=226, Percent_Identity=26.1061946902655, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI62472339, Length=402, Percent_Identity=24.8756218905473, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24667955, Length=402, Percent_Identity=24.8756218905473, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24667959, Length=402, Percent_Identity=24.8756218905473, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24648257, Length=199, Percent_Identity=25.1256281407035, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21355225, Length=442, Percent_Identity=22.1719457013575, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 64696; Mature: 64565

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDDESECCVFTRGLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIAC
CCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCHHHHH
FFLVFCKERTSHMGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKES
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCC
KNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDELKERIAISNPDMIVTERYKL
CCCEEEEEEHHHHHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHCCCCCCEEEEECEEC
NDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
CCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLE
HHHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TENISVMYTVPTMLESLYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGA
CCCEEEEEEHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHEECCCC
SELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEVQKGEIGTVYVKSDQFFMGYI
CHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEECHHCCHHHCCCCEEEEEECCCEEEHHH
IDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPA
HHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCC
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKSFCLQRLSSFKIPKEWYFVDEIPYTNSG
CCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
KIARMEAKSIIENQEKIYE
CEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GDDESECCVFTRGLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIAC
CCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCHHHHH
FFLVFCKERTSHMGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVANWLNEKES
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCC
KNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDELKERIAISNPDMIVTERYKL
CCCEEEEEEHHHHHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHCCCCCCEEEEECEEC
NDLPDEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
CCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLE
HHHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TENISVMYTVPTMLESLYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGA
CCCEEEEEEHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHEECCCC
SELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEVQKGEIGTVYVKSDQFFMGYI
CHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEECHHCCHHHCCCCEEEEEECCCEEEHHH
IDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPA
HHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCC
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKSFCLQRLSSFKIPKEWYFVDEIPYTNSG
CCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
KIARMEAKSIIENQEKIYE
CEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]