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The map label for this gene is gltB [H]

Identifier: 52141902

GI number: 52141902

Start: 3480357

End: 3481925

Strand: Reverse

Name: gltB [H]

Synonym: BCZK3348

Alternate gene names: 52141902

Gene position: 3481925-3480357 (Counterclockwise)

Preceding gene: 52141889

Following gene: 52141901

Centisome position: 65.69

GC content: 37.67

Gene sequence:

>1569_bases
ATGAGTGAAACGCTACTCGTTATTATTAGTATATTACTATTATTACTGTTGCTTTTAATCGTCTTTTTTATTATCACATT
TTTTATAAAAAAACGGACGCATCATTCTATTTTAAAACTACATCCATACTTAGGAAGAATGCGTTACTTACTGGAAAAGA
TAGGGCCGGAATTCCGGCAATATTGGTTTGATCACGATACAGATGGAAAACCTTTTTCACGTTACGACTTTCAAAGTGTT
ATGTTTTTAGCGAAGTATCGCTCTGAAATACTTGGTTTTGGGTCGAAACGAGATTTTGAAGCATCTGGTTATTATATTGC
TAATACGTTGTTTCCTGTATTAACAGAAGAATTAAGTGTGAATGTAACGCAAGAACGTGAAGGGAAAAAGTATGTGATTC
ATAAAGAAGGATTATTTTCACGAAGAGAAAAATTGACAGCGGATACAACAAATCTTTGGTTATACGAAGAAGACGATGCA
ATTATCGTCGGTGAAAATCGTAAATATCCATGGAAACTGTATGGCATGTTTGGAGCATCAGCAACTTCTTACGGTTCGAT
TGGTGAAAATTATATTTTAGCGAGTGGGTTTGGAGCGAAAATGGCTGGCGGTTCGTGGATTAATACTGGCGAAGGTGGCG
TTATTCCAGAACATTTACATACAGGGGCAAATATCGTTGCACAAATTGGCCCAGGGTTATTTGGATATCGTGATGAGGAC
GGTAATTTTTCGATGGAGAAATTTATGGGGAAAGCGAAAGAGAGTAATATTAAAGCATTTGAATTGAAATTTGGGCAAGG
TGCGAAAATACGTGGTGGTCATTTAGAGGGGCAAAAAGTAAACGAGAAAATTGCTTTTGTTCGGAATGTACGAGAGGGAG
AGACAATTAATTCACCGAATCGATTTTCATTTTTAAATAATGCAGCAGAGACACTTTATTTTATTCAACAGTTGCAAGAA
AACGGCGGTAAACCAGTTGGGATGAAAATTGTGATTGGACAACAGGAGCCGTTGGAAAATTTATTCAAAACGATGAAAGA
GTTAAACATCTATCCGGATTTCATTACAATTGATGGTTCAGAAGGTGGATCAGGAGCAACGTATAAATCGATGGCAGATA
GTATGGGAATGCCGCTTATTCCAGCATTATTGACGTTTATTGATACAGCAAATCATTATGATATTCGGGATAAATTCAAA
GTGTTTGCTGCCGGGAAATTAATTACACCAGATAAAGTGGCAATCGCTTTAGCTATTGGTGCAGATGCTGTAAATTCAGC
GCGTGGTTTTATGATGGCAAGCGGCTGTATAATGGCGCTTCAATGTAATTCTGGCCAATGTCCATCTGGTGTGGCCACGA
CGAATCCGCACTATCAAAAAGCGTTAGATCCGTATGAGAAGAAGTGGCGAGTTATGAATTATATTATTAGTATGAGATAT
AGTTTGTTTTCGTTAGCAGCAGCAGCGGGAGTAAAGAGTCCACGTCATTTAAAGAGAGATCATATTGTATTTAAAGATGA
GATGGGGCGGGTAGTTCCTCTCTCAGAATTGTTTCCTACTAGTAAATAG

Upstream 100 bases:

>100_bases
GACGCGAAAGAGCATCTTGCTACATTGATTTGAATAAAGATAAAAGGAGAACACATGGAGTGTTCTCCTTTTATTATGGG
ATTTCATAGGGGGAAGCGAG

Downstream 100 bases:

>100_bases
AAGTAAGCTATGTTTAATAGATTAAAAGTCATACATTTGTATGGCTTATTTTTATTAAAAAAATAGGTATTTTATTTTCT
GAATATTTTTACTTTAATTT

Product: ferredoxin-dependent glutamate synthase

Products: L-glutamine; 2-oxoglutarate; reduced ferredoxin

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSETLLVIISILLLLLLLLIVFFIITFFIKKRTHHSILKLHPYLGRMRYLLEKIGPEFRQYWFDHDTDGKPFSRYDFQSV
MFLAKYRSEILGFGSKRDFEASGYYIANTLFPVLTEELSVNVTQEREGKKYVIHKEGLFSRREKLTADTTNLWLYEEDDA
IIVGENRKYPWKLYGMFGASATSYGSIGENYILASGFGAKMAGGSWINTGEGGVIPEHLHTGANIVAQIGPGLFGYRDED
GNFSMEKFMGKAKESNIKAFELKFGQGAKIRGGHLEGQKVNEKIAFVRNVREGETINSPNRFSFLNNAAETLYFIQQLQE
NGGKPVGMKIVIGQQEPLENLFKTMKELNIYPDFITIDGSEGGSGATYKSMADSMGMPLIPALLTFIDTANHYDIRDKFK
VFAAGKLITPDKVAIALAIGADAVNSARGFMMASGCIMALQCNSGQCPSGVATTNPHYQKALDPYEKKWRVMNYIISMRY
SLFSLAAAAGVKSPRHLKRDHIVFKDEMGRVVPLSELFPTSK

Sequences:

>Translated_522_residues
MSETLLVIISILLLLLLLLIVFFIITFFIKKRTHHSILKLHPYLGRMRYLLEKIGPEFRQYWFDHDTDGKPFSRYDFQSV
MFLAKYRSEILGFGSKRDFEASGYYIANTLFPVLTEELSVNVTQEREGKKYVIHKEGLFSRREKLTADTTNLWLYEEDDA
IIVGENRKYPWKLYGMFGASATSYGSIGENYILASGFGAKMAGGSWINTGEGGVIPEHLHTGANIVAQIGPGLFGYRDED
GNFSMEKFMGKAKESNIKAFELKFGQGAKIRGGHLEGQKVNEKIAFVRNVREGETINSPNRFSFLNNAAETLYFIQQLQE
NGGKPVGMKIVIGQQEPLENLFKTMKELNIYPDFITIDGSEGGSGATYKSMADSMGMPLIPALLTFIDTANHYDIRDKFK
VFAAGKLITPDKVAIALAIGADAVNSARGFMMASGCIMALQCNSGQCPSGVATTNPHYQKALDPYEKKWRVMNYIISMRY
SLFSLAAAAGVKSPRHLKRDHIVFKDEMGRVVPLSELFPTSK
>Mature_521_residues
SETLLVIISILLLLLLLLIVFFIITFFIKKRTHHSILKLHPYLGRMRYLLEKIGPEFRQYWFDHDTDGKPFSRYDFQSVM
FLAKYRSEILGFGSKRDFEASGYYIANTLFPVLTEELSVNVTQEREGKKYVIHKEGLFSRREKLTADTTNLWLYEEDDAI
IVGENRKYPWKLYGMFGASATSYGSIGENYILASGFGAKMAGGSWINTGEGGVIPEHLHTGANIVAQIGPGLFGYRDEDG
NFSMEKFMGKAKESNIKAFELKFGQGAKIRGGHLEGQKVNEKIAFVRNVREGETINSPNRFSFLNNAAETLYFIQQLQEN
GGKPVGMKIVIGQQEPLENLFKTMKELNIYPDFITIDGSEGGSGATYKSMADSMGMPLIPALLTFIDTANHYDIRDKFKV
FAAGKLITPDKVAIALAIGADAVNSARGFMMASGCIMALQCNSGQCPSGVATTNPHYQKALDPYEKKWRVMNYIISMRYS
LFSLAAAAGVKSPRHLKRDHIVFKDEMGRVVPLSELFPTSK

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI308199519, Length=209, Percent_Identity=29.1866028708134, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17570289, Length=322, Percent_Identity=26.7080745341615, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=300, Percent_Identity=24.6666666666667, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28574881, Length=346, Percent_Identity=25.4335260115607, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24665539, Length=346, Percent_Identity=25.4335260115607, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: 1.4.7.1

Molecular weight: Translated: 58477; Mature: 58346

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETLLVIISILLLLLLLLIVFFIITFFIKKRTHHSILKLHPYLGRMRYLLEKIGPEFRQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHH
YWFDHDTDGKPFSRYDFQSVMFLAKYRSEILGFGSKRDFEASGYYIANTLFPVLTEELSV
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHEE
NVTQEREGKKYVIHKEGLFSRREKLTADTTNLWLYEEDDAIIVGENRKYPWKLYGMFGAS
EEEECCCCCEEEEECCCHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCEEEEEEECCC
ATSYGSIGENYILASGFGAKMAGGSWINTGEGGVIPEHLHTGANIVAQIGPGLFGYRDED
CCCCCCCCCCEEEECCCCCCCCCCCEEECCCCCCCHHHHHCCCHHHHHHCCCCCCEECCC
GNFSMEKFMGKAKESNIKAFELKFGQGAKIRGGHLEGQKVNEKIAFVRNVREGETINSPN
CCCCHHHHHCHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
RFSFLNNAAETLYFIQQLQENGGKPVGMKIVIGQQEPLENLFKTMKELNIYPDFITIDGS
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECC
EGGSGATYKSMADSMGMPLIPALLTFIDTANHYDIRDKFKVFAAGKLITPDKVAIALAIG
CCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHEEEECCCCCCCCCEEEEEEEC
ADAVNSARGFMMASGCIMALQCNSGQCPSGVATTNPHYQKALDPYEKKWRVMNYIISMRY
CHHHHHHCCCEEECCEEEEEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
SLFSLAAAAGVKSPRHLKRDHIVFKDEMGRVVPLSELFPTSK
HHHHHHHHHCCCCCHHHHHCCEEEHHCCCCEEEHHHHCCCCC
>Mature Secondary Structure 
SETLLVIISILLLLLLLLIVFFIITFFIKKRTHHSILKLHPYLGRMRYLLEKIGPEFRQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHH
YWFDHDTDGKPFSRYDFQSVMFLAKYRSEILGFGSKRDFEASGYYIANTLFPVLTEELSV
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHEE
NVTQEREGKKYVIHKEGLFSRREKLTADTTNLWLYEEDDAIIVGENRKYPWKLYGMFGAS
EEEECCCCCEEEEECCCHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCEEEEEEECCC
ATSYGSIGENYILASGFGAKMAGGSWINTGEGGVIPEHLHTGANIVAQIGPGLFGYRDED
CCCCCCCCCCEEEECCCCCCCCCCCEEECCCCCCCHHHHHCCCHHHHHHCCCCCCEECCC
GNFSMEKFMGKAKESNIKAFELKFGQGAKIRGGHLEGQKVNEKIAFVRNVREGETINSPN
CCCCHHHHHCHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
RFSFLNNAAETLYFIQQLQENGGKPVGMKIVIGQQEPLENLFKTMKELNIYPDFITIDGS
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECC
EGGSGATYKSMADSMGMPLIPALLTFIDTANHYDIRDKFKVFAAGKLITPDKVAIALAIG
CCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHEEEECCCCCCCCCEEEEEEEC
ADAVNSARGFMMASGCIMALQCNSGQCPSGVATTNPHYQKALDPYEKKWRVMNYIISMRY
CHHHHHHCCCEEECCEEEEEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
SLFSLAAAAGVKSPRHLKRDHIVFKDEMGRVVPLSELFPTSK
HHHHHHHHHCCCCCHHHHHCCEEEHHCCCCEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; oxidized ferredoxin

Specific reaction: 2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377; 8969499 [H]