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The map label for this gene is nemA [H]

Identifier: 52141889

GI number: 52141889

Start: 3481993

End: 3483120

Strand: Reverse

Name: nemA [H]

Synonym: BCZK3349

Alternate gene names: 52141889

Gene position: 3483120-3481993 (Counterclockwise)

Preceding gene: 52141890

Following gene: 52141902

Centisome position: 65.71

GC content: 38.48

Gene sequence:

>1128_bases
ATGACAAGTTCAAATAATAAAGCGGCAAGTATTTATGAAGGAACAAATGTGAATGCTGGGGGATCTTTTGTAAGTATTGA
AGAAACGAAAATTTTTAATCCAATTCAAATTGGATCTTGGCCTCTTCGTAACCGTATAGCAATGGCACCAATGACAAGAT
GCTTTGCGGACAATGAAACTGGAGCGGTGGGTGCTGATGTAGTGGAATACTATAGAAAACGTGCAGCTGATGGAGTTGGA
TTAATTATTACAGAAGGAATCGTTATTAGCGCAAGAGCAAAAGGAAACCCAGGGGTACCAGGAATTTATACACAAGAACA
AATTGATTCGTGGAAACCTGTTACAGAGGCAGTACATAAAGAAGGCGGGACAATTATTGCTCAAATATGGCATGTTGGTC
GTATGAGTCATCATGAAATAATTGGTGGGAAAATGCCGCAAGCACCGTCTGCAATCGCTGCAGAGGGAAATGTTCCACGT
TTTCGCAAACCATTCGATACACCAGAAGCAATGACATTAGAAGAAATACAAGAAGTTATTAGTCAATACGCACAAGCTGC
AAAAAATGCGATTGAAGCTGGGTTTGATGGAGTAGAAATTCACGGTGCACACGGATATTTAATTGATCAATTTACTTATG
AGTTTGCAAACAAGCGTACAGATAAATATGGCGGTGACTTAAAGCAAAGGTTAACGTTTATGAAAGAAGTAACTGAGGCT
GTAATAGAAGCAATTGGAGCTGATAAAACACTTCTTCGTTTCTCTGCTTTTAAAGGTGATAATCCTACTTATATGTGGGA
AAACCCTGAACTGGCAATTGAAACATTCGTAAATATGTTTAAAGAAGTTGGATTAACGATGATTCACCCTTCAACGATGA
GTTATACGCAAGTGATTGCTGACGGTAAGAATTTTCATCAATTAGTAAGAAAATATTGGGATGGTGCGATTGTTGGGGTT
GGTAATTTGAATCCGAAAGAGGCAGAAGAAGCGTTGCAAGAAGGAACAATTGATGTAGCTGCATTCGGAAGACCTTTAAT
TGCTAATCCGGATTTTGTTCATCGAATTAAAAGTGCTGAAAGTTTAGTTGAATATGACGCGAAAGAGCATCTTGCTACAT
TGATTTGA

Upstream 100 bases:

>100_bases
AATATTCGAATAATCTCTGGTTAAAGCGTATAAATTGACGTTACTATCTAAAAGTAAGTATGATGCGATTGTAGTAAGAA
TTTTTTTAGGGGGAAGAAAA

Downstream 100 bases:

>100_bases
ATAAAGATAAAAGGAGAACACATGGAGTGTTCTCCTTTTATTATGGGATTTCATAGGGGGAAGCGAGATGAGTGAAACGC
TACTCGTTATTATTAGTATA

Product: Oye family NADH-dependent flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTSSNNKAASIYEGTNVNAGGSFVSIEETKIFNPIQIGSWPLRNRIAMAPMTRCFADNETGAVGADVVEYYRKRAADGVG
LIITEGIVISARAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGKMPQAPSAIAAEGNVPR
FRKPFDTPEAMTLEEIQEVISQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEA
VIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMSYTQVIADGKNFHQLVRKYWDGAIVGV
GNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI

Sequences:

>Translated_375_residues
MTSSNNKAASIYEGTNVNAGGSFVSIEETKIFNPIQIGSWPLRNRIAMAPMTRCFADNETGAVGADVVEYYRKRAADGVG
LIITEGIVISARAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGKMPQAPSAIAAEGNVPR
FRKPFDTPEAMTLEEIQEVISQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEA
VIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMSYTQVIADGKNFHQLVRKYWDGAIVGV
GNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI
>Mature_374_residues
TSSNNKAASIYEGTNVNAGGSFVSIEETKIFNPIQIGSWPLRNRIAMAPMTRCFADNETGAVGADVVEYYRKRAADGVGL
IITEGIVISARAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGKMPQAPSAIAAEGNVPRF
RKPFDTPEAMTLEEIQEVISQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEAV
IEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMSYTQVIADGKNFHQLVRKYWDGAIVGVG
NLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=341, Percent_Identity=38.7096774193548, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI1789463, Length=345, Percent_Identity=31.304347826087, Blast_Score=141, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17565138, Length=349, Percent_Identity=28.9398280802292, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI72001454, Length=359, Percent_Identity=23.6768802228412, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=310, Percent_Identity=24.8387096774194, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=191, Percent_Identity=29.8429319371728, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=271, Percent_Identity=27.6752767527675, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=339, Percent_Identity=25.0737463126844, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=215, Percent_Identity=28.8372093023256, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=366, Percent_Identity=31.6939890710383, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321973, Length=364, Percent_Identity=31.3186813186813, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 41210; Mature: 41079

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSNNKAASIYEGTNVNAGGSFVSIEETKIFNPIQIGSWPLRNRIAMAPMTRCFADNET
CCCCCCCEEEEECCCCCCCCCCEEEEEHHHCCCCEECCCCCCCCCCHHHHHHHHHCCCCC
GAVGADVVEYYRKRAADGVGLIITEGIVISARAKGNPGVPGIYTQEQIDSWKPVTEAVHK
CCCHHHHHHHHHHHCCCCCEEEEECCEEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHH
EGGTIIAQIWHVGRMSHHEIIGGKMPQAPSAIAAEGNVPRFRKPFDTPEAMTLEEIQEVI
CCCEEEEHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEA
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHH
VIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMSYTQVIA
HHHHHCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
DGKNFHQLVRKYWDGAIVGVGNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAE
CCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHH
SLVEYDAKEHLATLI
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSSNNKAASIYEGTNVNAGGSFVSIEETKIFNPIQIGSWPLRNRIAMAPMTRCFADNET
CCCCCCEEEEECCCCCCCCCCEEEEEHHHCCCCEECCCCCCCCCCHHHHHHHHHCCCCC
GAVGADVVEYYRKRAADGVGLIITEGIVISARAKGNPGVPGIYTQEQIDSWKPVTEAVHK
CCCHHHHHHHHHHHCCCCCEEEEECCEEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHH
EGGTIIAQIWHVGRMSHHEIIGGKMPQAPSAIAAEGNVPRFRKPFDTPEAMTLEEIQEVI
CCCEEEEHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFANKRTDKYGGDLKQRLTFMKEVTEA
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHH
VIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMSYTQVIA
HHHHHCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
DGKNFHQLVRKYWDGAIVGVGNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAE
CCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHH
SLVEYDAKEHLATLI
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]