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The map label for this gene is spoVAF [H]

Identifier: 52141428

GI number: 52141428

Start: 3954136

End: 3955608

Strand: Reverse

Name: spoVAF [H]

Synonym: BCZK3820

Alternate gene names: 52141428

Gene position: 3955608-3954136 (Counterclockwise)

Preceding gene: 52141427

Following gene: 52141429

Centisome position: 74.62

GC content: 35.23

Gene sequence:

>1473_bases
ATGACGAAGCCTAAAAAAATAGATATTCCTATTTCAACTTTCATAAGTGACAATGAAAATTATTTAAAACAAACGGCTGG
ATTAGGCGTCACATTTGATGTTGGTATTCGTAAATTTCAAATTTTGAATAAAGAAATTGCAGTGTTATTTGTAAATGGAC
TTTGTGATACAAATTATATTATTCCTATTTTAGAAGAAGCTGTGGATACGAATGAAATAAGGGATGTTGAAGAAGATACT
GTAAAGCTTTTAGAGAATCGTTTAATTCATCAGCAAGTAAGTAAAGTGAAAACGATGGATGAAGTAATGCTTCAAGCGTT
ATCAGGGCTTATTGTTATATTTGTGGAGGGCGAAACAGAAGCGTTCGTGATAGATGTTCGTAGCTATCCAGGACGAACAC
CGACAGAGCCAGATACCGAAAAAGTAGTACGCGGTGCGAGGGATGGATTTGTTGAAAATATCGTTGTAAATACAGCGTTA
ATTCGTAGAAGAATTCGAGATCCACGTCTTCGAAATGAAATTATTCGAGTAGGGGATAGATCACAAACGGATATTTGTAT
TACATATGTACAAGATGTTGCAAATCCAGATTTAGTAAAGATTATAAAACAAGAATTAAATAACATCGATGTCGATGGCA
TTACGATGGCGGATAAAACAGTGGAAGAATTTGTTGTAAAGCAAAGCTATAATCCATTCCCGCTTATTCGATATACAGAA
AGACCGGATGTAGCAGCGAATCATTTATTAGAAGGACATGTTCTAGTACTAGTTGATACATCACCGAGTGCTATGATTAC
GCCAACAACATATTTTCATCATTTACAGCATGCAGAAGAGTTTAGACAAAATCCAGCTGTCGGAACATTTTTACGTTGGG
TACGCTTTTTAGGTGTCTTATTCTCCTTGTTTTTACTACCGTTTTGGTTAGTGTTTGTATTTGATCCAACTCTTTTACCA
GAAAATCTTGCTTTTATTGGACCGACTAAGATGACACATTTACCAATTCTATTACAGGTGATAATGGCAGAAGTAGGACT
TGAATTTTTAAGGATGGCAGCCATTCATACTCCAACGCCGTTATCGTCTGCAGCAGGATTAATTTCTGCCATATTAATTG
GTCAAATAGCAATTGATGTAGGTTTGTTTGTACCAGAAGTTATTTTGTATGTAGCAGTTTCTATGATCGGGGCATATGCA
ACACCGAGTTATGAACTAGGACTCGGAAATAAGGTTGGAAAATTGTTTGTTATTATTTTGACAGGCCTCTTCCATGAGAT
GGGATTTGTAATTGGTATGACGATATTGATTTTATTTTTAACGTCTATAAAAAGTTTACAAACACCTTACTTATGGCCAT
TTTTACCATTTGATTGGGGCGCATTAACAAAAATTCTACTCCGTCCGACGATGTCTAGTTTAAAAGTACGACCAAGTATT
GTAAGACCTCAAAATGTAAAAAGACAAAAATAA

Upstream 100 bases:

>100_bases
GTTGGTGATATTGGTAAGATGTGTGGAAACGATGAATGGGAGAGAGGTTCACCAATTACGAAAAAAGCAATTCAGTTAAT
TTTGGAAAGGAGTGGATTTT

Downstream 100 bases:

>100_bases
ACGGGATTATACACCCGTTTATTTTTTTAGCAAAAATTTATTGTTTTTTAACAAAAATCAAACATTTTTTGTGTACACTT
AGAGTAGATATTGTGTATGG

Product: stage V sporulation protein AF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTKPKKIDIPISTFISDNENYLKQTAGLGVTFDVGIRKFQILNKEIAVLFVNGLCDTNYIIPILEEAVDTNEIRDVEEDT
VKLLENRLIHQQVSKVKTMDEVMLQALSGLIVIFVEGETEAFVIDVRSYPGRTPTEPDTEKVVRGARDGFVENIVVNTAL
IRRRIRDPRLRNEIIRVGDRSQTDICITYVQDVANPDLVKIIKQELNNIDVDGITMADKTVEEFVVKQSYNPFPLIRYTE
RPDVAANHLLEGHVLVLVDTSPSAMITPTTYFHHLQHAEEFRQNPAVGTFLRWVRFLGVLFSLFLLPFWLVFVFDPTLLP
ENLAFIGPTKMTHLPILLQVIMAEVGLEFLRMAAIHTPTPLSSAAGLISAILIGQIAIDVGLFVPEVILYVAVSMIGAYA
TPSYELGLGNKVGKLFVIILTGLFHEMGFVIGMTILILFLTSIKSLQTPYLWPFLPFDWGALTKILLRPTMSSLKVRPSI
VRPQNVKRQK

Sequences:

>Translated_490_residues
MTKPKKIDIPISTFISDNENYLKQTAGLGVTFDVGIRKFQILNKEIAVLFVNGLCDTNYIIPILEEAVDTNEIRDVEEDT
VKLLENRLIHQQVSKVKTMDEVMLQALSGLIVIFVEGETEAFVIDVRSYPGRTPTEPDTEKVVRGARDGFVENIVVNTAL
IRRRIRDPRLRNEIIRVGDRSQTDICITYVQDVANPDLVKIIKQELNNIDVDGITMADKTVEEFVVKQSYNPFPLIRYTE
RPDVAANHLLEGHVLVLVDTSPSAMITPTTYFHHLQHAEEFRQNPAVGTFLRWVRFLGVLFSLFLLPFWLVFVFDPTLLP
ENLAFIGPTKMTHLPILLQVIMAEVGLEFLRMAAIHTPTPLSSAAGLISAILIGQIAIDVGLFVPEVILYVAVSMIGAYA
TPSYELGLGNKVGKLFVIILTGLFHEMGFVIGMTILILFLTSIKSLQTPYLWPFLPFDWGALTKILLRPTMSSLKVRPSI
VRPQNVKRQK
>Mature_489_residues
TKPKKIDIPISTFISDNENYLKQTAGLGVTFDVGIRKFQILNKEIAVLFVNGLCDTNYIIPILEEAVDTNEIRDVEEDTV
KLLENRLIHQQVSKVKTMDEVMLQALSGLIVIFVEGETEAFVIDVRSYPGRTPTEPDTEKVVRGARDGFVENIVVNTALI
RRRIRDPRLRNEIIRVGDRSQTDICITYVQDVANPDLVKIIKQELNNIDVDGITMADKTVEEFVVKQSYNPFPLIRYTER
PDVAANHLLEGHVLVLVDTSPSAMITPTTYFHHLQHAEEFRQNPAVGTFLRWVRFLGVLFSLFLLPFWLVFVFDPTLLPE
NLAFIGPTKMTHLPILLQVIMAEVGLEFLRMAAIHTPTPLSSAAGLISAILIGQIAIDVGLFVPEVILYVAVSMIGAYAT
PSYELGLGNKVGKLFVIILTGLFHEMGFVIGMTILILFLTSIKSLQTPYLWPFLPFDWGALTKILLRPTMSSLKVRPSIV
RPQNVKRQK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gerABKA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004995 [H]

Pfam domain/function: PF03323 GerA [H]

EC number: NA

Molecular weight: Translated: 55107; Mature: 54976

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPKKIDIPISTFISDNENYLKQTAGLGVTFDVGIRKFQILNKEIAVLFVNGLCDTNYI
CCCCCEECCCHHHHHCCCHHHHHHHHCCCEEECCCHHHHHHHCCCEEEEEEECCCCCCCH
IPILEEAVDTNEIRDVEEDTVKLLENRLIHQQVSKVKTMDEVMLQALSGLIVIFVEGETE
HHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
AFVIDVRSYPGRTPTEPDTEKVVRGARDGFVENIVVNTALIRRRIRDPRLRNEIIRVGDR
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
SQTDICITYVQDVANPDLVKIIKQELNNIDVDGITMADKTVEEFVVKQSYNPFPLIRYTE
CCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCCCEEEECC
RPDVAANHLLEGHVLVLVDTSPSAMITPTTYFHHLQHAEEFRQNPAVGTFLRWVRFLGVL
CCCHHHHHHCCCEEEEEEECCCCCEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FSLFLLPFWLVFVFDPTLLPENLAFIGPTKMTHLPILLQVIMAEVGLEFLRMAAIHTPTP
HHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LSSAAGLISAILIGQIAIDVGLFVPEVILYVAVSMIGAYATPSYELGLGNKVGKLFVIIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHH
TGLFHEMGFVIGMTILILFLTSIKSLQTPYLWPFLPFDWGALTKILLRPTMSSLKVRPSI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VRPQNVKRQK
CCCCCCCCCC
>Mature Secondary Structure 
TKPKKIDIPISTFISDNENYLKQTAGLGVTFDVGIRKFQILNKEIAVLFVNGLCDTNYI
CCCCEECCCHHHHHCCCHHHHHHHHCCCEEECCCHHHHHHHCCCEEEEEEECCCCCCCH
IPILEEAVDTNEIRDVEEDTVKLLENRLIHQQVSKVKTMDEVMLQALSGLIVIFVEGETE
HHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
AFVIDVRSYPGRTPTEPDTEKVVRGARDGFVENIVVNTALIRRRIRDPRLRNEIIRVGDR
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
SQTDICITYVQDVANPDLVKIIKQELNNIDVDGITMADKTVEEFVVKQSYNPFPLIRYTE
CCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCCCEEEECC
RPDVAANHLLEGHVLVLVDTSPSAMITPTTYFHHLQHAEEFRQNPAVGTFLRWVRFLGVL
CCCHHHHHHCCCEEEEEEECCCCCEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FSLFLLPFWLVFVFDPTLLPENLAFIGPTKMTHLPILLQVIMAEVGLEFLRMAAIHTPTP
HHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LSSAAGLISAILIGQIAIDVGLFVPEVILYVAVSMIGAYATPSYELGLGNKVGKLFVIIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHH
TGLFHEMGFVIGMTILILFLTSIKSLQTPYLWPFLPFDWGALTKILLRPTMSSLKVRPSI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VRPQNVKRQK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 7934829; 1368705; 3926949 [H]