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The map label for this gene is ywtE [H]

Identifier: 52141429

GI number: 52141429

Start: 3953236

End: 3954021

Strand: Reverse

Name: ywtE [H]

Synonym: BCZK3819

Alternate gene names: 52141429

Gene position: 3954021-3953236 (Counterclockwise)

Preceding gene: 52141428

Following gene: 52141432

Centisome position: 74.59

GC content: 36.77

Gene sequence:

>786_bases
ATGATTAAATTATTTGTAAGTGATTTAGATGAAACGCTCGTTTACAATGTGAATGATATGCAAAAAGAAGATGAACGTGC
AATTTGTTGGTTAGCAGAAAAAGGGACAAATATTTGTTTTGCCTCGGGCCGCTTTACTCATCGAATTGATGAAGTCGTAA
ATAGGTTTGCATTCCCATACTACACAACTAGTTTAAATGGAGCGACGATGTTACTACCAGATGGGAAAATATTTCATGAA
TCAAGTTTTGAAGATGGGATTGCCCAGGAAATATATCGATATATCCATAAAAAGGGACTAGCAGATATTGTTTGTGCAAA
TGAGCAACGGTATACAAAAAGAAAGAATGAACATCACCATACTTTTGAAACATATATGGGCGTGCATATTGCTGAGATAG
AAGCATTGGAAGAGGAATTTGGTAAAACTGTACACCCTGCGAAATTGTTTGTTTTTGGGGAAGAAGAGACAATTGAAGCG
TTAGATCAAGAATTACGATATACATTTCAGAGCGAAGCAGAAGTTTTCATGTCTGGTAAACGCTATGTTGACATTATGCC
AAGGGGAGTAAGTAAAGGAAGTGCTTTAAGGAGACTAATGGAACATTTGCAAATTGAAGCAAATGAGGTTGCATGCATTG
GAGATTCTTTCAATGATATTTCCATGTTTGAAGTAACCCCGCATTCCTTCACTTTGCATCATGCTCACCCATATGTGAAG
GAGAAGGCAAATCATATAGTTCGTTCGGTCGAAGAAGCTATTATGAAATTGCCATTACTTGCATAA

Upstream 100 bases:

>100_bases
CCGTTTATTTTTTTAGCAAAAATTTATTGTTTTTTAACAAAAATCAAACATTTTTTGTGTACACTTAGAGTAGATATTGT
GTATGGAAGGGGAAGAGGAC

Downstream 100 bases:

>100_bases
GAAAGAAGCTCGTCAGTGACGAGCTTCTTTTTACTTGAATAACGATTTAACAAAATCAATGATACTTGAGAAGAAATCTT
TAACTTTATCTAGGAAACTT

Product: HAD-superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MIKLFVSDLDETLVYNVNDMQKEDERAICWLAEKGTNICFASGRFTHRIDEVVNRFAFPYYTTSLNGATMLLPDGKIFHE
SSFEDGIAQEIYRYIHKKGLADIVCANEQRYTKRKNEHHHTFETYMGVHIAEIEALEEEFGKTVHPAKLFVFGEEETIEA
LDQELRYTFQSEAEVFMSGKRYVDIMPRGVSKGSALRRLMEHLQIEANEVACIGDSFNDISMFEVTPHSFTLHHAHPYVK
EKANHIVRSVEEAIMKLPLLA

Sequences:

>Translated_261_residues
MIKLFVSDLDETLVYNVNDMQKEDERAICWLAEKGTNICFASGRFTHRIDEVVNRFAFPYYTTSLNGATMLLPDGKIFHE
SSFEDGIAQEIYRYIHKKGLADIVCANEQRYTKRKNEHHHTFETYMGVHIAEIEALEEEFGKTVHPAKLFVFGEEETIEA
LDQELRYTFQSEAEVFMSGKRYVDIMPRGVSKGSALRRLMEHLQIEANEVACIGDSFNDISMFEVTPHSFTLHHAHPYVK
EKANHIVRSVEEAIMKLPLLA
>Mature_261_residues
MIKLFVSDLDETLVYNVNDMQKEDERAICWLAEKGTNICFASGRFTHRIDEVVNRFAFPYYTTSLNGATMLLPDGKIFHE
SSFEDGIAQEIYRYIHKKGLADIVCANEQRYTKRKNEHHHTFETYMGVHIAEIEALEEEFGKTVHPAKLFVFGEEETIEA
LDQELRYTFQSEAEVFMSGKRYVDIMPRGVSKGSALRRLMEHLQIEANEVACIGDSFNDISMFEVTPHSFTLHHAHPYVK
EKANHIVRSVEEAIMKLPLLA

Specific function: Unknown

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081741, Length=247, Percent_Identity=20.2429149797571, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI2367265, Length=266, Percent_Identity=24.0601503759398, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI48994981, Length=246, Percent_Identity=22.3577235772358, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 30085; Mature: 30085

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLFVSDLDETLVYNVNDMQKEDERAICWLAEKGTNICFASGRFTHRIDEVVNRFAFPY
CEEEEEECCCHHEEECCHHHCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCE
YTTSLNGATMLLPDGKIFHESSFEDGIAQEIYRYIHKKGLADIVCANEQRYTKRKNEHHH
EEECCCCEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCH
TFETYMGVHIAEIEALEEEFGKTVHPAKLFVFGEEETIEALDQELRYTFQSEAEVFMSGK
HHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCHHHHHCCC
RYVDIMPRGVSKGSALRRLMEHLQIEANEVACIGDSFNDISMFEVTPHSFTLHHAHPYVK
EEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCEEEEECCCCHHH
EKANHIVRSVEEAIMKLPLLA
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIKLFVSDLDETLVYNVNDMQKEDERAICWLAEKGTNICFASGRFTHRIDEVVNRFAFPY
CEEEEEECCCHHEEECCHHHCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCE
YTTSLNGATMLLPDGKIFHESSFEDGIAQEIYRYIHKKGLADIVCANEQRYTKRKNEHHH
EEECCCCEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCH
TFETYMGVHIAEIEALEEEFGKTVHPAKLFVFGEEETIEALDQELRYTFQSEAEVFMSGK
HHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCHHHHHCCC
RYVDIMPRGVSKGSALRRLMEHLQIEANEVACIGDSFNDISMFEVTPHSFTLHHAHPYVK
EEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCEEEEECCCCHHH
EKANHIVRSVEEAIMKLPLLA
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]