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The map label for this gene is pbuE [H]

Identifier: 52140516

GI number: 52140516

Start: 4835182

End: 4836366

Strand: Reverse

Name: pbuE [H]

Synonym: BCZK4742

Alternate gene names: 52140516

Gene position: 4836366-4835182 (Counterclockwise)

Preceding gene: 52140515

Following gene: 52140517

Centisome position: 91.24

GC content: 38.9

Gene sequence:

>1185_bases
GTGGGGAAAGACTTGAATTTTCGTGTGTATATTTTAGCTATCGCGGCTTTCGTAGTCGGAACGGTTGAGCTAATTATAGG
AGGAACGCTTGATTTAGTTGCCGATGATTTAGGAGTATCTATTAGTGCAGCTGGTCAGCTTATCACAATATTTTCAGTAG
TATTTGCTTTATCAGGTCCTGTTTTATTAGCATTAACAGGGAAATTTGAAAGAAAAACATTGTATGTTGGGGCATTATCA
ATTTTCTTAATCGGAAATATTATTTCAGCATTTAGTGTGAATTACGGGATGTTAATGTTCTCAAGGGTGATTTGTGCGGC
GAGTGGTTCGCTTATTATTGCACTGTCAGTAACACTTGCTTCTAGCGTTGTAGAACCGCATTTTCGTGCGCGTGCAATTG
GAATCATTTTCATGGGGATTAGTGGATCACTTGTACTTGGAGTACCGCTTGGACTTGTACTCGGAAATGCATACGGATGG
CGTGCACCATTTGTACTCATTTCGATCTTAACAGTTATGGCAATCGCTTGTATTTCATTATTCTTAACGAAAGTACCACC
GACATCAGTATTATCAATAAGAGAACAAATTGCTACGTTAAAAGATAAAAAAATCGTAAGTGCACAGTTAACATCATTCT
TATTTTTAACGGGTCATTTAACACTATATGCATATTTAACACCATTTTTAAAAGATGTGATGCATGTGGAAGCGAATTGG
ATTAGTGTATTTTACTTCATCTTCGGAATCGCAGCAGTAATTGGCGGCGGATTCGGAGGATGGCTTGCGGATAAATGGGG
ATCGAAGAAAAGTATTATTTCAATTATTATTGTATTTGCGTGTGCGATCTTCCTATTGCCATTAATGACATTCTCATTCC
CGCTGTTCATTATTATGATGGGACTTTGGAGTATGCTGAGCTGGGCTATTTCACCAGCGCAACAAAACTATTTGATTGAA
ATTGCACCAGAATCAGCTGGTATTCAGCAAAGTTTAAATAACTCTGCTCTTCACTTAGGGATTGCGCTCGGTTCAACAGT
AGGCGGAGTTGTTATTGAGAAATCATCTGTTATATATAATGCTTGGGTAGGCGGCGGCTTTATTATATTAGCGTTGCTTT
GTGCGATTTTCTCGATTACGAGAGGACGTTCTGCTCGGTTTGCGAAGGAGGAATCTATCGTTTGA

Upstream 100 bases:

>100_bases
GTAGGCGTATCATACGCCTACCTTTTTTGCTGTAAAAAAATTGTACTTGTGTTCAACTTCTATTTTGGTTACTATAGTAG
TAGTAACCAAAATAGAAAGC

Downstream 100 bases:

>100_bases
TAGATTCTTTTTTATTTGTAAAAAATGGAAGGTTTAGTAGGTAGGTGGAGAATTTGTATGTAGCGAGGTGATGTATATGT
ATACGAATTTTTCAATTATT

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MGKDLNFRVYILAIAAFVVGTVELIIGGTLDLVADDLGVSISAAGQLITIFSVVFALSGPVLLALTGKFERKTLYVGALS
IFLIGNIISAFSVNYGMLMFSRVICAASGSLIIALSVTLASSVVEPHFRARAIGIIFMGISGSLVLGVPLGLVLGNAYGW
RAPFVLISILTVMAIACISLFLTKVPPTSVLSIREQIATLKDKKIVSAQLTSFLFLTGHLTLYAYLTPFLKDVMHVEANW
ISVFYFIFGIAAVIGGGFGGWLADKWGSKKSIISIIIVFACAIFLLPLMTFSFPLFIIMMGLWSMLSWAISPAQQNYLIE
IAPESAGIQQSLNNSALHLGIALGSTVGGVVIEKSSVIYNAWVGGGFIILALLCAIFSITRGRSARFAKEESIV

Sequences:

>Translated_394_residues
MGKDLNFRVYILAIAAFVVGTVELIIGGTLDLVADDLGVSISAAGQLITIFSVVFALSGPVLLALTGKFERKTLYVGALS
IFLIGNIISAFSVNYGMLMFSRVICAASGSLIIALSVTLASSVVEPHFRARAIGIIFMGISGSLVLGVPLGLVLGNAYGW
RAPFVLISILTVMAIACISLFLTKVPPTSVLSIREQIATLKDKKIVSAQLTSFLFLTGHLTLYAYLTPFLKDVMHVEANW
ISVFYFIFGIAAVIGGGFGGWLADKWGSKKSIISIIIVFACAIFLLPLMTFSFPLFIIMMGLWSMLSWAISPAQQNYLIE
IAPESAGIQQSLNNSALHLGIALGSTVGGVVIEKSSVIYNAWVGGGFIILALLCAIFSITRGRSARFAKEESIV
>Mature_393_residues
GKDLNFRVYILAIAAFVVGTVELIIGGTLDLVADDLGVSISAAGQLITIFSVVFALSGPVLLALTGKFERKTLYVGALSI
FLIGNIISAFSVNYGMLMFSRVICAASGSLIIALSVTLASSVVEPHFRARAIGIIFMGISGSLVLGVPLGLVLGNAYGWR
APFVLISILTVMAIACISLFLTKVPPTSVLSIREQIATLKDKKIVSAQLTSFLFLTGHLTLYAYLTPFLKDVMHVEANWI
SVFYFIFGIAAVIGGGFGGWLADKWGSKKSIISIIIVFACAIFLLPLMTFSFPLFIIMMGLWSMLSWAISPAQQNYLIEI
APESAGIQQSLNNSALHLGIALGSTVGGVVIEKSSVIYNAWVGGGFIILALLCAIFSITRGRSARFAKEESIV

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine and 6-mercaptopurine. Therefore plays a role in maintaining t

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=332, Percent_Identity=29.8192771084337, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787808, Length=232, Percent_Identity=27.5862068965517, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786595, Length=349, Percent_Identity=20.6303724928367, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693198, Length=234, Percent_Identity=22.6495726495727, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42023; Mature: 41892

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKDLNFRVYILAIAAFVVGTVELIIGGTLDLVADDLGVSISAAGQLITIFSVVFALSGP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
VLLALTGKFERKTLYVGALSIFLIGNIISAFSVNYGMLMFSRVICAASGSLIIALSVTLA
EEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEHHHHH
SSVVEPHFRARAIGIIFMGISGSLVLGVPLGLVLGNAYGWRAPFVLISILTVMAIACISL
HHHHCHHHHHEEEEEEEECCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
FLTKVPPTSVLSIREQIATLKDKKIVSAQLTSFLFLTGHLTLYAYLTPFLKDVMHVEANW
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISVFYFIFGIAAVIGGGFGGWLADKWGSKKSIISIIIVFACAIFLLPLMTFSFPLFIIMM
HHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLWSMLSWAISPAQQNYLIEIAPESAGIQQSLNNSALHLGIALGSTVGGVVIEKSSVIYN
HHHHHHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEE
AWVGGGFIILALLCAIFSITRGRSARFAKEESIV
ECCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
GKDLNFRVYILAIAAFVVGTVELIIGGTLDLVADDLGVSISAAGQLITIFSVVFALSGP
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
VLLALTGKFERKTLYVGALSIFLIGNIISAFSVNYGMLMFSRVICAASGSLIIALSVTLA
EEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEHHHHH
SSVVEPHFRARAIGIIFMGISGSLVLGVPLGLVLGNAYGWRAPFVLISILTVMAIACISL
HHHHCHHHHHEEEEEEEECCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
FLTKVPPTSVLSIREQIATLKDKKIVSAQLTSFLFLTGHLTLYAYLTPFLKDVMHVEANW
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISVFYFIFGIAAVIGGGFGGWLADKWGSKKSIISIIIVFACAIFLLPLMTFSFPLFIIMM
HHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLWSMLSWAISPAQQNYLIEIAPESAGIQQSLNNSALHLGIALGSTVGGVVIEKSSVIYN
HHHHHHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEE
AWVGGGFIILALLCAIFSITRGRSARFAKEESIV
ECCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA