Click here to switch to the map view.

The map label for this gene is smpB

Identifier: 52140442

GI number: 52140442

Start: 4909749

End: 4910216

Strand: Reverse

Name: smpB

Synonym: BCZK4817

Alternate gene names: 52140442

Gene position: 4910216-4909749 (Counterclockwise)

Preceding gene: 52140440

Following gene: 52140441

Centisome position: 92.63

GC content: 38.68

Gene sequence:

>468_bases
ATGCCAAAAGGTTCAGGTAAGGTTATTGCACAAAATAAAAAAGCATTTCATGATTATTTCATCGAAGAAACATACGAAGC
AGGGCTTGTCCTTCAAGGAACGGAAATTAAGTCGATTCGCGCTGGACGCGTGAACTTGAAAGATGCGTTTGCACGTGTAC
ATAATGGTGAAGTATGGGTTCATAATATGCATATTAGTACGTACGAACAAGGAAATCGTTTCAACCACGATCCGCTTCGC
ACGAGAAAGTTACTTCTTCATAAAAAAGAAATTGAGAAGTTAGCGGGTGCTTCAAAAGAAACAGGATATGCACTAGTTCC
AGTTAGAATCTATTTGAAAAATGGATTTGCGAAAATGGCACTTGGTTTAGCAAAAGGTAAGAAACAATACGATAAACGTC
ACGATTTAAAAGAGAAAGAAGCTAAACGTGAAATTGCACGCGCGTTCCGTGATCGCCAAAAGATGTAA

Upstream 100 bases:

>100_bases
CCTCAAGGTCAGAAGCTCCATCCACCTCACATTCTGAATGAGCCACTTTCGCTTTTTGTTCACATTTGTTACAATAGTAA
AAATAGAGGAGGGACGTTAT

Downstream 100 bases:

>100_bases
TACAGATATTTACATTTGTAAAACGGCGCGCGTATGTTATAATAACTTATGTAAGATTGTTGCACATTGAAAAACAGCTC
TTAGTAGCTATCACGAAATT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MPKGSGKVIAQNKKAFHDYFIEETYEAGLVLQGTEIKSIRAGRVNLKDAFARVHNGEVWVHNMHISTYEQGNRFNHDPLR
TRKLLLHKKEIEKLAGASKETGYALVPVRIYLKNGFAKMALGLAKGKKQYDKRHDLKEKEAKREIARAFRDRQKM

Sequences:

>Translated_155_residues
MPKGSGKVIAQNKKAFHDYFIEETYEAGLVLQGTEIKSIRAGRVNLKDAFARVHNGEVWVHNMHISTYEQGNRFNHDPLR
TRKLLLHKKEIEKLAGASKETGYALVPVRIYLKNGFAKMALGLAKGKKQYDKRHDLKEKEAKREIARAFRDRQKM
>Mature_154_residues
PKGSGKVIAQNKKAFHDYFIEETYEAGLVLQGTEIKSIRAGRVNLKDAFARVHNGEVWVHNMHISTYEQGNRFNHDPLRT
RKLLLHKKEIEKLAGASKETGYALVPVRIYLKNGFAKMALGLAKGKKQYDKRHDLKEKEAKREIARAFRDRQKM

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=150, Percent_Identity=43.3333333333333, Blast_Score=131, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_BACAA (C3P072)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002869324.1
- ProteinModelPortal:   C3P072
- SMR:   C3P072
- EnsemblBacteria:   EBBACT00000125585
- GeneID:   7852808
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_5363
- GeneTree:   EBGT00050000002020
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17862; Mature: 17731

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKGSGKVIAQNKKAFHDYFIEETYEAGLVLQGTEIKSIRAGRVNLKDAFARVHNGEVWV
CCCCCCCEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEE
HNMHISTYEQGNRFNHDPLRTRKLLLHKKEIEKLAGASKETGYALVPVRIYLKNGFAKMA
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHH
LGLAKGKKQYDKRHDLKEKEAKREIARAFRDRQKM
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PKGSGKVIAQNKKAFHDYFIEETYEAGLVLQGTEIKSIRAGRVNLKDAFARVHNGEVWV
CCCCCCEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEE
HNMHISTYEQGNRFNHDPLRTRKLLLHKKEIEKLAGASKETGYALVPVRIYLKNGFAKMA
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHH
LGLAKGKKQYDKRHDLKEKEAKREIARAFRDRQKM
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA