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The map label for this gene is tpiA [H]

Identifier: 52140432

GI number: 52140432

Start: 4919604

End: 4920359

Strand: Reverse

Name: tpiA [H]

Synonym: BCZK4826

Alternate gene names: 52140432

Gene position: 4920359-4919604 (Counterclockwise)

Preceding gene: 52140431

Following gene: 52140433

Centisome position: 92.82

GC content: 43.65

Gene sequence:

>756_bases
ATGCGTAAACCAATTATCGCAGGTAACTGGAAAATGAATAAAACTCTATCTGAAGCAGTTAGCTTCGTAGAGGAAGTTAA
AGGTCAAATCCCAGCAGCTTCAGCTGTTGATGCAGTAGTGTGCTCTCCAGCTCTATTCTTAGAGCGCCTTGTAGCAGCGA
CTGAAGGAACTGATTTACAAGTAGGTGCACAAAACATGCACTTCGAAAAAAATGGTGCATTCACTGGCGAAATTAGCCCA
GTAGCACTTAGCGACTTAAAAGTAGGCTACGTAGTACTTGGTCACTCTGAGCGTCGTGAAATGTTTGCTGAAACAGATGA
GTCAGTAAACAAAAAGACTATCGCAGCATTTGAACATGGTTTAACACCAATCGTATGTTGTGGTGAGACTTTAGAAGAGC
GCGAAAGCGGAAAAACATTTGATCTAGTAGCAGGTCAAGTGACAAAAGCACTTGCAGGTTTAACAGAAGAGCAAGTTAAA
GCAACTGTTATCGCTTATGAGCCAATCTGGGCTATCGGTACAGGTAAATCTTCTTCTTCTGCAGATGCAAACGAAGTATG
TGCGCACATCCGTAAAGTTGTTGCAGAAGCTGTTTCTCCAGAAGCTGCAGAAGCTGTTCGTATTCAATACGGCGGTAGCG
TAAAACCAGAAAACATTAAAGAGTACATGGCACAATCTGACATCGACGGCGCTTTAGTTGGCGGTGCTAGCTTAGAGCCT
GCTTCGTTCTTAGGTCTTCTGGGGGCGGTAAAATGA

Upstream 100 bases:

>100_bases
GCGGTGCGTCATTAGAATTCATGGAAGGTAAAGAGCTTCCAGGTGTAGTTTGTCTTAACGACAAATAAGTAGCAGCCAAA
GAAAAAGGACGGTGCAAAGC

Downstream 100 bases:

>100_bases
GAAAGCCAACAGCTTTAATCATTCTTGACGGTTTCGGACTTCGTGAAGAAACTTACGGGAATGCTGTAGCGCAAGCTAAG
AAACCTAATTTTGATGGTTA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRKPIIAGNWKMNKTLSEAVSFVEEVKGQIPAASAVDAVVCSPALFLERLVAATEGTDLQVGAQNMHFEKNGAFTGEISP
VALSDLKVGYVVLGHSERREMFAETDESVNKKTIAAFEHGLTPIVCCGETLEERESGKTFDLVAGQVTKALAGLTEEQVK
ATVIAYEPIWAIGTGKSSSSADANEVCAHIRKVVAEAVSPEAAEAVRIQYGGSVKPENIKEYMAQSDIDGALVGGASLEP
ASFLGLLGAVK

Sequences:

>Translated_251_residues
MRKPIIAGNWKMNKTLSEAVSFVEEVKGQIPAASAVDAVVCSPALFLERLVAATEGTDLQVGAQNMHFEKNGAFTGEISP
VALSDLKVGYVVLGHSERREMFAETDESVNKKTIAAFEHGLTPIVCCGETLEERESGKTFDLVAGQVTKALAGLTEEQVK
ATVIAYEPIWAIGTGKSSSSADANEVCAHIRKVVAEAVSPEAAEAVRIQYGGSVKPENIKEYMAQSDIDGALVGGASLEP
ASFLGLLGAVK
>Mature_251_residues
MRKPIIAGNWKMNKTLSEAVSFVEEVKGQIPAASAVDAVVCSPALFLERLVAATEGTDLQVGAQNMHFEKNGAFTGEISP
VALSDLKVGYVVLGHSERREMFAETDESVNKKTIAAFEHGLTPIVCCGETLEERESGKTFDLVAGQVTKALAGLTEEQVK
ATVIAYEPIWAIGTGKSSSSADANEVCAHIRKVVAEAVSPEAAEAVRIQYGGSVKPENIKEYMAQSDIDGALVGGASLEP
ASFLGLLGAVK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI226529917, Length=251, Percent_Identity=42.6294820717131, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI4507645, Length=251, Percent_Identity=42.6294820717131, Blast_Score=174, Evalue=7e-44,
Organism=Escherichia coli, GI1790353, Length=243, Percent_Identity=41.9753086419753, Blast_Score=194, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17536593, Length=249, Percent_Identity=44.578313253012, Blast_Score=187, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6320255, Length=243, Percent_Identity=41.1522633744856, Blast_Score=178, Evalue=6e-46,
Organism=Drosophila melanogaster, GI28572008, Length=252, Percent_Identity=42.8571428571429, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28572006, Length=252, Percent_Identity=42.8571428571429, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28572004, Length=252, Percent_Identity=42.8571428571429, Blast_Score=178, Evalue=3e-45,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 26468; Mature: 26468

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPIIAGNWKMNKTLSEAVSFVEEVKGQIPAASAVDAVVCSPALFLERLVAATEGTDLQ
CCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VGAQNMHFEKNGAFTGEISPVALSDLKVGYVVLGHSERREMFAETDESVNKKTIAAFEHG
ECCCCCEEECCCCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
LTPIVCCGETLEERESGKTFDLVAGQVTKALAGLTEEQVKATVIAYEPIWAIGTGKSSSS
CCEEEECCHHHHHHHCCCEEEHHHHHHHHHHHCCCHHHHEEEEEEECCEEEEECCCCCCC
ADANEVCAHIRKVVAEAVSPEAAEAVRIQYGGSVKPENIKEYMAQSDIDGALVGGASLEP
CCHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCCH
ASFLGLLGAVK
HHHHHHHHCCC
>Mature Secondary Structure
MRKPIIAGNWKMNKTLSEAVSFVEEVKGQIPAASAVDAVVCSPALFLERLVAATEGTDLQ
CCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VGAQNMHFEKNGAFTGEISPVALSDLKVGYVVLGHSERREMFAETDESVNKKTIAAFEHG
ECCCCCEEECCCCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
LTPIVCCGETLEERESGKTFDLVAGQVTKALAGLTEEQVKATVIAYEPIWAIGTGKSSSS
CCEEEECCHHHHHHHCCCEEEHHHHHHHHHHHCCCHHHHEEEEEEECCEEEEECCCCCCC
ADANEVCAHIRKVVAEAVSPEAAEAVRIQYGGSVKPENIKEYMAQSDIDGALVGGASLEP
CCHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCCCCH
ASFLGLLGAVK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]