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The map label for this gene is pgk [H]

Identifier: 52140431

GI number: 52140431

Start: 4920392

End: 4921576

Strand: Reverse

Name: pgk [H]

Synonym: BCZK4827

Alternate gene names: 52140431

Gene position: 4921576-4920392 (Counterclockwise)

Preceding gene: 52140430

Following gene: 52140432

Centisome position: 92.84

GC content: 41.27

Gene sequence:

>1185_bases
ATGAACAAAAAATCAATTCGTGACGTAGATTTAAAAGGTAAGCGTGTATTTTGCCGCGTTGACTTCAACGTACCTATGAA
AGAAGGCAAAATTACAGATGAAACTCGTATCCGTGCAGCTCTTCCTACAATTCAATATTTAGTAGAGCAAGGTGCGAAAG
TTATTTTAGCAAGCCACTTAGGCCGTCCAAAAGGTCAAGTAGTAGAAGAAATGCGTTTAACTCCAGTAGCAGCACGTTTA
GGCGAGCTTCTTGGTAAAGACGTTAAAAAAGCAGACGAAGCATTCGGACCAGCTGTACAAGAAATGGTTGCAGCAATGAA
CGAAGGCGACGTATTAGTTCTTGAAAACGTACGTTTCTATGCGGGCGAAGAAAAGAACGATGCAGAACTTGCGAAAGAAT
TTGCAGCTCTTGCTGACATCTTCGTAAACGATGCATTCGGTGCAGCTCACCGTGCTCATGCTTCTACAGCAGGAATCGCA
GACTACCTACCAGCAGTATCTGGTCTATTAATGGAAAAAGAGTTAGAAGTATTAGGTAAAGCACTTTCTAACCCAGAACG
TCCATTCACAGCTATCATCGGTGGTGCGAAAGTAAAAGATAAAATCGGTGTAATTCGTCATCTATTAGACAAAGTAGATA
ACTTAATCATCGGTGGCGGACTTGCTTACACATTCGTTAAAGCTTTAGGTCATGAAATTGGTCTATCTCTATGTGAAGAC
GACAAGATTGAACTAGCTAAAGAATTTATGCAACTTGCAAAAGAAAAAGGCGTAAACTTCTACATGCCAGTTGATGTTGT
AATCACTGAGGAATTCTCTGAAACTGCAACAACTAAAATCGTTGGTATCGACTCTATCCCTTCTAACTGGGAAGGCGTGG
ACATCGGTCCTAAAACACGTGAAATTTATGCAGATGTAATTAAAAACTCTAAGCTTGTTGTATGGAATGGACCAATGGGT
GTATTCGAAATGACTCCATTCGCAGAAGGTACAAAAGCAGTAGGACAAGCATTAGCAGATGCAGAAGGTACATACTCTGT
TATCGGCGGTGGTGACTCTGCAGCAGCTGTTGAAAAATTCGGTATGGCTGATAAAATGAGCCACATTTCTACTGGCGGCG
GTGCGTCATTAGAATTCATGGAAGGTAAAGAGCTTCCAGGTGTAGTTTGTCTTAACGACAAATAA

Upstream 100 bases:

>100_bases
AGGGTTTGTTCCCTCTCTCTCTTCTTTCGTTTTAAAAGCAAATGTGTTAAGCTTTTGAGTGGGTTTTGTCAATCCTAACT
AGTAGTCGGAGGGAAATCCA

Downstream 100 bases:

>100_bases
GTAGCAGCCAAAGAAAAAGGACGGTGCAAAGCATGCGTAAACCAATTATCGCAGGTAACTGGAAAATGAATAAAACTCTA
TCTGAAGCAGTTAGCTTCGT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQVVEEMRLTPVAARL
GELLGKDVKKADEAFGPAVQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVSGLLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK

Sequences:

>Translated_394_residues
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQVVEEMRLTPVAARL
GELLGKDVKKADEAFGPAVQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVSGLLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK
>Mature_394_residues
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQVVEEMRLTPVAARL
GELLGKDVKKADEAFGPAVQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVSGLLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=414, Percent_Identity=47.3429951690821, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=46.7312348668281, Blast_Score=347, Evalue=1e-95,
Organism=Escherichia coli, GI1789294, Length=398, Percent_Identity=46.4824120603015, Blast_Score=345, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=48.3009708737864, Blast_Score=366, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319857, Length=412, Percent_Identity=49.5145631067961, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17136394, Length=411, Percent_Identity=47.4452554744526, Blast_Score=342, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=45.3012048192771, Blast_Score=331, Evalue=6e-91,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42330; Mature: 42330

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHC
GRPKGQVVEEMRLTPVAARLGELLGKDVKKADEAFGPAVQEMVAAMNEGDVLVLENVRFY
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECEEEE
AGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIADYLPAVSGLLMEKELEVLGK
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALSNPERPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCCC
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTR
CHHHHHHHHHHHHHHCCCEEEEEEEEEEECCHHHHHHEEEEEECCCCCCCCCCCCCCCHH
EIYADVIKNSKLVVWNGPMGVFEMTPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKF
HHHHHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
GMADKMSHISTGGGASLEFMEGKELPGVVCLNDK
CCHHHHHHCCCCCCCEEEEECCCCCCCEEEECCC
>Mature Secondary Structure
MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHC
GRPKGQVVEEMRLTPVAARLGELLGKDVKKADEAFGPAVQEMVAAMNEGDVLVLENVRFY
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECEEEE
AGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIADYLPAVSGLLMEKELEVLGK
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALSNPERPFTAIIGGAKVKDKIGVIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCCC
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTR
CHHHHHHHHHHHHHHCCCEEEEEEEEEEECCHHHHHHEEEEEECCCCCCCCCCCCCCCHH
EIYADVIKNSKLVVWNGPMGVFEMTPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKF
HHHHHHHCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
GMADKMSHISTGGGASLEFMEGKELPGVVCLNDK
CCHHHHHHCCCCCCCEEEEECCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA