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The map label for this gene is hydN1 [H]

Identifier: 51894426

GI number: 51894426

Start: 3516359

End: 3516958

Strand: Reverse

Name: hydN1 [H]

Synonym: STH3292

Alternate gene names: 51894426

Gene position: 3516958-3516359 (Counterclockwise)

Preceding gene: 51894427

Following gene: 51894424

Centisome position: 98.62

GC content: 71.33

Gene sequence:

>600_bases
ATGACGAAGAGCGCGTTCGTGCTGGCCGACCCGACCAAGTGCATCGGGTGCCAGTCCTGCGAGATCGCCTGCGCCGTCGC
CCATCTGGGCACATCGGTCACCACGGCCGGCGAGATGAAGATTCCCTTCCAACCCCGGCTGCGGGTGGTGCGCACCTCCC
GGGTGACCATGCCGATCCAGTGCCGCCACTGCGAGGACGCTCCGTGCGCCAACGCCTGCCCGGTCGGGGCCATCGTGCGG
CAGGACGGCGTGGTGCTGGTGAAGCAGGAGAGGTGCATCGGCTGCAAGACCTGCGTGCTGGCCTGCCCCTTCGGCGCCAT
GGACATGGTGCCGGCGCTGGAGAACGGCGCGCGGGTGGAACAGCCGGGGCTGAAGATGGACACGCCCGACGGCAAGGTCC
CCAAGGAGCGGTACGTGGCGCACAAGTGCGATCTCTGCATCGGCCGGCCGGGCGGCCCGGCGTGCGTCGAGGTCTGCCTG
GCCCGGGCCCTGACGCTGGTCAGGCGCACCGCCATCGACCAGCGGGTGCGGTCGCGGCGCGTTGCGACCGCGTCGGGCAT
CGCCCGGTCGATGTCGCCGAGGGCCGCGGCGCGATCGTAA

Upstream 100 bases:

>100_bases
CGGACATCAAGAAGATCTACAAGGAGTTCCTGGGCAAGCCGTTGGGCGAGAAATCGCATCACCTGCTGCACACCAGCTAC
ACGCCGAGGAGGCGCAAGGC

Downstream 100 bases:

>100_bases
GCCCACGCGGACGGGAGCATGACTGTACATGCCGCACCGCGTTTGGGGGCTGTCCCGAGAGTCATCCGAGATGACCTCGG
GACAGCCCCCTTTCCCGCAC

Product: electron transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MTKSAFVLADPTKCIGCQSCEIACAVAHLGTSVTTAGEMKIPFQPRLRVVRTSRVTMPIQCRHCEDAPCANACPVGAIVR
QDGVVLVKQERCIGCKTCVLACPFGAMDMVPALENGARVEQPGLKMDTPDGKVPKERYVAHKCDLCIGRPGGPACVEVCL
ARALTLVRRTAIDQRVRSRRVATASGIARSMSPRAAARS

Sequences:

>Translated_199_residues
MTKSAFVLADPTKCIGCQSCEIACAVAHLGTSVTTAGEMKIPFQPRLRVVRTSRVTMPIQCRHCEDAPCANACPVGAIVR
QDGVVLVKQERCIGCKTCVLACPFGAMDMVPALENGARVEQPGLKMDTPDGKVPKERYVAHKCDLCIGRPGGPACVEVCL
ARALTLVRRTAIDQRVRSRRVATASGIARSMSPRAAARS
>Mature_198_residues
TKSAFVLADPTKCIGCQSCEIACAVAHLGTSVTTAGEMKIPFQPRLRVVRTSRVTMPIQCRHCEDAPCANACPVGAIVRQ
DGVVLVKQERCIGCKTCVLACPFGAMDMVPALENGARVEQPGLKMDTPDGKVPKERYVAHKCDLCIGRPGGPACVEVCLA
RALTLVRRTAIDQRVRSRRVATASGIARSMSPRAAARS

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788811, Length=181, Percent_Identity=43.646408839779, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1789067, Length=181, Percent_Identity=44.7513812154696, Blast_Score=143, Evalue=9e-36,
Organism=Escherichia coli, GI2367245, Length=175, Percent_Identity=43.4285714285714, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1789079, Length=190, Percent_Identity=39.4736842105263, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI87082180, Length=188, Percent_Identity=38.2978723404255, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI87082179, Length=182, Percent_Identity=40.1098901098901, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI87082114, Length=197, Percent_Identity=34.5177664974619, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787872, Length=176, Percent_Identity=30.6818181818182, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1789370, Length=223, Percent_Identity=26.0089686098655, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787122, Length=176, Percent_Identity=30.6818181818182, Blast_Score=70, Evalue=7e-14,
Organism=Escherichia coli, GI226510944, Length=171, Percent_Identity=28.6549707602339, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI1787749, Length=191, Percent_Identity=27.7486910994764, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI2367345, Length=174, Percent_Identity=29.3103448275862, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 21236; Mature: 21105

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
11.6 %Cys+Met (Translated Protein)
8.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
11.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSAFVLADPTKCIGCQSCEIACAVAHLGTSVTTAGEMKIPFQPRLRVVRTSRVTMPIQ
CCCCCEEEECCHHHCCCCCCHHHHHHHHHCCCCCCCCCEECCCCCCEEEEEECCEEEEEE
CRHCEDAPCANACPVGAIVRQDGVVLVKQERCIGCKTCVLACPFGAMDMVPALENGARVE
ECCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCHHHHEEECCCCHHHHHHHHCCCCEEC
QPGLKMDTPDGKVPKERYVAHKCDLCIGRPGGPACVEVCLARALTLVRRTAIDQRVRSRR
CCCCEECCCCCCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATASGIARSMSPRAAARS
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TKSAFVLADPTKCIGCQSCEIACAVAHLGTSVTTAGEMKIPFQPRLRVVRTSRVTMPIQ
CCCCEEEECCHHHCCCCCCHHHHHHHHHCCCCCCCCCEECCCCCCEEEEEECCEEEEEE
CRHCEDAPCANACPVGAIVRQDGVVLVKQERCIGCKTCVLACPFGAMDMVPALENGARVE
ECCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCHHHHEEECCCCHHHHHHHHCCCCEEC
QPGLKMDTPDGKVPKERYVAHKCDLCIGRPGGPACVEVCLARALTLVRRTAIDQRVRSRR
CCCCEECCCCCCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATASGIARSMSPRAAARS
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]