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The map label for this gene is metS1 [H]

Identifier: 51894387

GI number: 51894387

Start: 3470857

End: 3472812

Strand: Reverse

Name: metS1 [H]

Synonym: STH3252

Alternate gene names: 51894387

Gene position: 3472812-3470857 (Counterclockwise)

Preceding gene: 51894389

Following gene: 51894386

Centisome position: 97.38

GC content: 65.95

Gene sequence:

>1956_bases
ATGACCAGGTTCTACATCACGACGCCCATCTACTACCCCAGCAGCAAGCTGCACATCGGCCACTCTTACACGACCGTGGC
CGCGGACGCGCTGGCGCGCTACCACCGCCGGCTGGGGCATGAGACGTACTTCCTGACCGGCACGGACGAGCACGGTCAGA
AGATCGAGCGGGCGGCCCTTGCCGAGGGGAAGACCCCGATCCAGTTCGTGGACGAGATTGTGGCCGGCATCAAGGAACTC
TGGGCAGCCCTGCACATCTCCTACGACGACTTCATCCGCACCACCGAGGAGCGGCACAAGAAGGTCGTGCAGAAGATCTT
CAAGCAGCTGTACGACCAGGGCGACATCTACAAGTCCTCGTACGAGGGCTGGTACTGTACGCCGTGTGAGACGTTCTGGA
TCGAGAGCAAGCTGAAGGAGGGCAACCTCTGCCCGGACTGCGGCCGTCCGGTGGAGTGGACCAAGGACGAGAGCTATTTC
TTCCGCCTCTCCAAGTACGCGGACCGGCTGCTGCGGTACATTGAGGAGACCGACTTCATCCAGCCCGCGTCCCGCAAGAA
CGAGATGATCTCGTTCATCAACCAGGGGCTGGAGGACCTCTGCGTCTCCCGCAGCACGGTGCGTTGGGGCATTCCGGTGC
CCTTCGATGAGAAGCAGACGATCTACGTCTGGATCGACGCCCTCTCCAACTACATCTCCGCCCTGGGCTGGGGCACGGAG
AACGACGAACTGTACCGGAAGTTCTGGCCGGCCGACGTGCACCTGGTGGGTAAGGAGATCGTGCGGTTCCACACCATCAT
CTGGCCGTGCATCCTGATGGCGCTGGGCCTGCCGCTGCCCAAGAAGGTCTTCGGCCACGGCTGGCTGCTGTTCGGCACCC
AGGCCGAGAAGATGTCCAAGTCCAAGGGCAACGTGGTGGATCCCTTCGTCCTCATCGACCGGTACGGTGTGGACGCCGTC
CGGTACTACCTGCTGCGGGAGGTGCCCTTCGGCGCGGACGGCACCTACACCGAAGAGGCGTTCATCCTGCGCACCAACAA
CGACCTGGCCAACGACCTGGGCAACCTGCTTTCCCGCACGACGCAGATGATCAACAAGTTCAGCGACGGCCGGGTCCCCG
AGCCCGATCCGGCGAGCGACGACCGGATCCTGTCCGACCTGGCCGCCCAGGTGCTCCGGGAGTTCCAGGAGGCCATGGAT
CGGCTGGAGATCTCCTCGGCGCTGGTTGCCCTGTGGAAGCTGGTGGATCGGGCAAACAAGTATATTGACGAGCAGGCGCC
CTGGGCCCTGGCGCGGGATCCCGGCAGGGCCGGCCAGCTCCGCACGGTGCTGTACAACCTTGCCGAGACGCTGCGCCTGC
TCGGGGTGGCGCTCACGCCGTTCCTGGTCCAGACGCCGGAGCGGATCTGGGACCAGCTCGGCCTGGAGCCCGCACAGGTG
CGGACGCTCCCCTGGGAGGAGGCGGTCGCCTGGGGCGGCCTGAAGCCCGGCACCGTGATCCGCCGGGGCAGCCCGCTGTT
CCCGCGCATCGAGCTGCCGAAGGAGAACGGCGACTCGGTTGCGAAGGAGGGCAAGGCGCAGTCCGGCGAGCCCGCCGCCG
GGGCCGCTCAGCCGACGGAGGGCGTGGCGGAGATCGAATACGACGACTTCGCCAAGGTGGAGCTGAAGGTCGCGGAGGTC
GTGGAGGCCGAACGGATTCCCAAGGCCGACCGGCTGCTCCGCCTCGTCCTCCAGGTGGGCGAGGAGCGCCGGCAGATCGT
CTCCGGCATCGCGCAGCACTACGCGCCGGAGGAGCTGGTCGGCAAGCGGATCATCCTGGTGGCCAACCTGAAGCCGAAGA
AGCTTCGCGGGGTCGAATCCCACGGCATGCTGCTGGCCGCCACCGCGGAGGACGGAAGCCTCAGCCTGGTCACGCTCGAC
AAGCCGGACTTCCCCACCGGGATGCGCGTGAAGTAG

Upstream 100 bases:

>100_bases
CGAATAAGGGTGGTACCACGGGGTGCATGCCTCGTCCCTTGCACAGGGGGACGAGGCTTCTTGCGTTTACGGGGCAAGCT
TTGTCGAGGAGGGGTTCCGC

Downstream 100 bases:

>100_bases
GTCAAATCCAGTCTCCCCGGGCGCCTCGCAGCGGCGCCCGGGGAGATGAACTGAGAGAGGGACGCGGCATGTTGTTCAAC
ACGCATTCGCACGTGGACAC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 651; Mature: 650

Protein sequence:

>651_residues
MTRFYITTPIYYPSSKLHIGHSYTTVAADALARYHRRLGHETYFLTGTDEHGQKIERAALAEGKTPIQFVDEIVAGIKEL
WAALHISYDDFIRTTEERHKKVVQKIFKQLYDQGDIYKSSYEGWYCTPCETFWIESKLKEGNLCPDCGRPVEWTKDESYF
FRLSKYADRLLRYIEETDFIQPASRKNEMISFINQGLEDLCVSRSTVRWGIPVPFDEKQTIYVWIDALSNYISALGWGTE
NDELYRKFWPADVHLVGKEIVRFHTIIWPCILMALGLPLPKKVFGHGWLLFGTQAEKMSKSKGNVVDPFVLIDRYGVDAV
RYYLLREVPFGADGTYTEEAFILRTNNDLANDLGNLLSRTTQMINKFSDGRVPEPDPASDDRILSDLAAQVLREFQEAMD
RLEISSALVALWKLVDRANKYIDEQAPWALARDPGRAGQLRTVLYNLAETLRLLGVALTPFLVQTPERIWDQLGLEPAQV
RTLPWEEAVAWGGLKPGTVIRRGSPLFPRIELPKENGDSVAKEGKAQSGEPAAGAAQPTEGVAEIEYDDFAKVELKVAEV
VEAERIPKADRLLRLVLQVGEERRQIVSGIAQHYAPEELVGKRIILVANLKPKKLRGVESHGMLLAATAEDGSLSLVTLD
KPDFPTGMRVK

Sequences:

>Translated_651_residues
MTRFYITTPIYYPSSKLHIGHSYTTVAADALARYHRRLGHETYFLTGTDEHGQKIERAALAEGKTPIQFVDEIVAGIKEL
WAALHISYDDFIRTTEERHKKVVQKIFKQLYDQGDIYKSSYEGWYCTPCETFWIESKLKEGNLCPDCGRPVEWTKDESYF
FRLSKYADRLLRYIEETDFIQPASRKNEMISFINQGLEDLCVSRSTVRWGIPVPFDEKQTIYVWIDALSNYISALGWGTE
NDELYRKFWPADVHLVGKEIVRFHTIIWPCILMALGLPLPKKVFGHGWLLFGTQAEKMSKSKGNVVDPFVLIDRYGVDAV
RYYLLREVPFGADGTYTEEAFILRTNNDLANDLGNLLSRTTQMINKFSDGRVPEPDPASDDRILSDLAAQVLREFQEAMD
RLEISSALVALWKLVDRANKYIDEQAPWALARDPGRAGQLRTVLYNLAETLRLLGVALTPFLVQTPERIWDQLGLEPAQV
RTLPWEEAVAWGGLKPGTVIRRGSPLFPRIELPKENGDSVAKEGKAQSGEPAAGAAQPTEGVAEIEYDDFAKVELKVAEV
VEAERIPKADRLLRLVLQVGEERRQIVSGIAQHYAPEELVGKRIILVANLKPKKLRGVESHGMLLAATAEDGSLSLVTLD
KPDFPTGMRVK
>Mature_650_residues
TRFYITTPIYYPSSKLHIGHSYTTVAADALARYHRRLGHETYFLTGTDEHGQKIERAALAEGKTPIQFVDEIVAGIKELW
AALHISYDDFIRTTEERHKKVVQKIFKQLYDQGDIYKSSYEGWYCTPCETFWIESKLKEGNLCPDCGRPVEWTKDESYFF
RLSKYADRLLRYIEETDFIQPASRKNEMISFINQGLEDLCVSRSTVRWGIPVPFDEKQTIYVWIDALSNYISALGWGTEN
DELYRKFWPADVHLVGKEIVRFHTIIWPCILMALGLPLPKKVFGHGWLLFGTQAEKMSKSKGNVVDPFVLIDRYGVDAVR
YYLLREVPFGADGTYTEEAFILRTNNDLANDLGNLLSRTTQMINKFSDGRVPEPDPASDDRILSDLAAQVLREFQEAMDR
LEISSALVALWKLVDRANKYIDEQAPWALARDPGRAGQLRTVLYNLAETLRLLGVALTPFLVQTPERIWDQLGLEPAQVR
TLPWEEAVAWGGLKPGTVIRRGSPLFPRIELPKENGDSVAKEGKAQSGEPAAGAAQPTEGVAEIEYDDFAKVELKVAEVV
EAERIPKADRLLRLVLQVGEERRQIVSGIAQHYAPEELVGKRIILVANLKPKKLRGVESHGMLLAATAEDGSLSLVTLDK
PDFPTGMRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=508, Percent_Identity=37.992125984252, Blast_Score=317, Evalue=3e-86,
Organism=Homo sapiens, GI14043022, Length=559, Percent_Identity=29.1592128801431, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI4507947, Length=93, Percent_Identity=39.7849462365591, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1788432, Length=695, Percent_Identity=28.4892086330935, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1789455, Length=106, Percent_Identity=37.7358490566038, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1786861, Length=178, Percent_Identity=28.0898876404494, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI212645096, Length=515, Percent_Identity=33.9805825242718, Blast_Score=275, Evalue=8e-74,
Organism=Caenorhabditis elegans, GI71992781, Length=409, Percent_Identity=32.518337408313, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17540794, Length=576, Percent_Identity=26.9097222222222, Blast_Score=181, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321610, Length=515, Percent_Identity=33.2038834951456, Blast_Score=263, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6321704, Length=561, Percent_Identity=26.0249554367201, Blast_Score=143, Evalue=7e-35,
Organism=Drosophila melanogaster, GI21358483, Length=503, Percent_Identity=35.9840954274354, Blast_Score=309, Evalue=5e-84,
Organism=Drosophila melanogaster, GI24655311, Length=638, Percent_Identity=27.2727272727273, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI21355409, Length=178, Percent_Identity=28.0898876404494, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 73725; Mature: 73594

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFYITTPIYYPSSKLHIGHSYTTVAADALARYHRRLGHETYFLTGTDEHGQKIERAAL
CCEEEEECCEEECCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
AEGKTPIQFVDEIVAGIKELWAALHISYDDFIRTTEERHKKVVQKIFKQLYDQGDIYKSS
HCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YEGWYCTPCETFWIESKLKEGNLCPDCGRPVEWTKDESYFFRLSKYADRLLRYIEETDFI
CCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
QPASRKNEMISFINQGLEDLCVSRSTVRWGIPVPFDEKQTIYVWIDALSNYISALGWGTE
CCCHHHHHHHHHHHHCHHHHHHCCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHCCCCC
NDELYRKFWPADVHLVGKEIVRFHTIIWPCILMALGLPLPKKVFGHGWLLFGTQAEKMSK
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCHHHHHC
SKGNVVDPFVLIDRYGVDAVRYYLLREVPFGADGTYTEEAFILRTNNDLANDLGNLLSRT
CCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
TQMINKFSDGRVPEPDPASDDRILSDLAAQVLREFQEAMDRLEISSALVALWKLVDRANK
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIDEQAPWALARDPGRAGQLRTVLYNLAETLRLLGVALTPFLVQTPERIWDQLGLEPAQV
HHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHE
RTLPWEEAVAWGGLKPGTVIRRGSPLFPRIELPKENGDSVAKEGKAQSGEPAAGAAQPTE
ECCCHHHHHHCCCCCCCCEEECCCCCCCEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCC
GVAEIEYDDFAKVELKVAEVVEAERIPKADRLLRLVLQVGEERRQIVSGIAQHYAPEELV
CCEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
GKRIILVANLKPKKLRGVESHGMLLAATAEDGSLSLVTLDKPDFPTGMRVK
CCEEEEEECCCHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TRFYITTPIYYPSSKLHIGHSYTTVAADALARYHRRLGHETYFLTGTDEHGQKIERAAL
CEEEEECCEEECCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
AEGKTPIQFVDEIVAGIKELWAALHISYDDFIRTTEERHKKVVQKIFKQLYDQGDIYKSS
HCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YEGWYCTPCETFWIESKLKEGNLCPDCGRPVEWTKDESYFFRLSKYADRLLRYIEETDFI
CCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
QPASRKNEMISFINQGLEDLCVSRSTVRWGIPVPFDEKQTIYVWIDALSNYISALGWGTE
CCCHHHHHHHHHHHHCHHHHHHCCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHCCCCC
NDELYRKFWPADVHLVGKEIVRFHTIIWPCILMALGLPLPKKVFGHGWLLFGTQAEKMSK
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCHHHHHC
SKGNVVDPFVLIDRYGVDAVRYYLLREVPFGADGTYTEEAFILRTNNDLANDLGNLLSRT
CCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
TQMINKFSDGRVPEPDPASDDRILSDLAAQVLREFQEAMDRLEISSALVALWKLVDRANK
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIDEQAPWALARDPGRAGQLRTVLYNLAETLRLLGVALTPFLVQTPERIWDQLGLEPAQV
HHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHE
RTLPWEEAVAWGGLKPGTVIRRGSPLFPRIELPKENGDSVAKEGKAQSGEPAAGAAQPTE
ECCCHHHHHHCCCCCCCCEEECCCCCCCEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCC
GVAEIEYDDFAKVELKVAEVVEAERIPKADRLLRLVLQVGEERRQIVSGIAQHYAPEELV
CCEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
GKRIILVANLKPKKLRGVESHGMLLAATAEDGSLSLVTLDKPDFPTGMRVK
CCEEEEEECCCHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]