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The map label for this gene is rsmA

Identifier: 51894384

GI number: 51894384

Start: 3462470

End: 3463327

Strand: Reverse

Name: rsmA

Synonym: STH3249

Alternate gene names: 51894384

Gene position: 3463327-3462470 (Counterclockwise)

Preceding gene: 51894385

Following gene: 51894383

Centisome position: 97.12

GC content: 68.41

Gene sequence:

>858_bases
ATGGACTTGGCGAACCCGGGCGCGCTGAAGGCGCTGATGGCCCAATACGGCCTGCGGCCGCAGCACCGGCTGGGGCAGAA
CTTCCTCATTGACGGTAGGGTACTGGACGGCATCGTGTCGGCCGCCGGACTGGAACCGACCGACGTCGTGTTGGAGATCG
GTCCAGGGCTGGGCACGCTGACGCAGCGCCTGGCCGCGAAGGCGGGGCGCGTGGTCTGCGTCGAGTTGGACCGCGGGCTG
GTGCAGGTCCTGCACGATACCGTGCAGAAGGCTTACGACAACGTCGAGGTCATCCACGGTGACGCGGGCCGGATCGATTT
ACATAAACTGCTCGGGGAACGGCTGGCGCCGGGGCAGAAGGCCAAGGTGGTGGCGAACCTCCCCTACTACATCACGACGC
CGCTGGTCATGCGGCTCCTGGAGGAGGAGCTGCCGCTTTCCCACGTGGTGGTGATGGTTCAGAAGGAAGTGGCCGACCGC
ATGGTGTCGCCGCCGGGCTCCAAGGCCTATGGGGCCCTCAGCGTCGCGGTACAATATTACACGGAGCCGCGGATCGTGCT
CCGGGTGAGCCGGGCGTCCTTCATGCCGCAGCCGGAGGTGGACTCGGCCGTCGTCAGCCTGCGGTATCGGGAACGGCCGC
CCGTGGATGCGCCGCCTGAGGCGTTTTTCCGGGTGGTCCGGGCCGCCTTCGGCCAGCGGCGCAAGAGTCTGGTGAACGCG
CTGACGTCCCTGGGGGTGGAAAAGGCCGCGGTGCATGCGGCCCTGGAGGCGGCGGGCATCGACCCCGGCCGACGGGGCGA
GAGCCTGTCGCTGGAGGAGTTTGCGGCGGTGGCCCGAACCCTGTGGCAGCGCACCTGA

Upstream 100 bases:

>100_bases
GTGCGGCCGGTCACGGTCTATGTCCTGGGAGACTGAACACGGGCCAGGGGCCGTCGTCCGGCCCCTGGCTTCTTGCAATC
CCTGGTAAGGAGACGATTCC

Downstream 100 bases:

>100_bases
CCGGTTGGAGGGAGCACCTGTGGAGGATGGTAGATTCTACAGCCCGACCAGGGGACCGCTGACGTTGGATCAGGTGGTCG
AGGACCTGCTGGCGTTCATG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MDLANPGALKALMAQYGLRPQHRLGQNFLIDGRVLDGIVSAAGLEPTDVVLEIGPGLGTLTQRLAAKAGRVVCVELDRGL
VQVLHDTVQKAYDNVEVIHGDAGRIDLHKLLGERLAPGQKAKVVANLPYYITTPLVMRLLEEELPLSHVVVMVQKEVADR
MVSPPGSKAYGALSVAVQYYTEPRIVLRVSRASFMPQPEVDSAVVSLRYRERPPVDAPPEAFFRVVRAAFGQRRKSLVNA
LTSLGVEKAAVHAALEAAGIDPGRRGESLSLEEFAAVARTLWQRT

Sequences:

>Translated_285_residues
MDLANPGALKALMAQYGLRPQHRLGQNFLIDGRVLDGIVSAAGLEPTDVVLEIGPGLGTLTQRLAAKAGRVVCVELDRGL
VQVLHDTVQKAYDNVEVIHGDAGRIDLHKLLGERLAPGQKAKVVANLPYYITTPLVMRLLEEELPLSHVVVMVQKEVADR
MVSPPGSKAYGALSVAVQYYTEPRIVLRVSRASFMPQPEVDSAVVSLRYRERPPVDAPPEAFFRVVRAAFGQRRKSLVNA
LTSLGVEKAAVHAALEAAGIDPGRRGESLSLEEFAAVARTLWQRT
>Mature_285_residues
MDLANPGALKALMAQYGLRPQHRLGQNFLIDGRVLDGIVSAAGLEPTDVVLEIGPGLGTLTQRLAAKAGRVVCVELDRGL
VQVLHDTVQKAYDNVEVIHGDAGRIDLHKLLGERLAPGQKAKVVANLPYYITTPLVMRLLEEELPLSHVVVMVQKEVADR
MVSPPGSKAYGALSVAVQYYTEPRIVLRVSRASFMPQPEVDSAVVSLRYRERPPVDAPPEAFFRVVRAAFGQRRKSLVNA
LTSLGVEKAAVHAALEAAGIDPGRRGESLSLEEFAAVARTLWQRT

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=238, Percent_Identity=34.8739495798319, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI156415992, Length=278, Percent_Identity=31.294964028777, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1786236, Length=264, Percent_Identity=37.5, Blast_Score=147, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI25141369, Length=296, Percent_Identity=29.3918918918919, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25146882, Length=219, Percent_Identity=33.7899543378995, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324989, Length=236, Percent_Identity=31.7796610169492, Blast_Score=108, Evalue=8e-25,
Organism=Drosophila melanogaster, GI21358017, Length=220, Percent_Identity=32.7272727272727, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21357273, Length=241, Percent_Identity=30.2904564315353, Blast_Score=107, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_SYMTH (Q67JB9)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_077075.1
- ProteinModelPortal:   Q67JB9
- SMR:   Q67JB9
- GeneID:   2979734
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH3249
- NMPDR:   fig|292459.1.peg.3119
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- BioCyc:   STHE292459:STH3249-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30963; Mature: 30963

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 27-27 BINDING 29-29 BINDING 54-54 BINDING 75-75 BINDING 101-101 BINDING 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLANPGALKALMAQYGLRPQHRLGQNFLIDGRVLDGIVSAAGLEPTDVVLEIGPGLGTL
CCCCCHHHHHHHHHHHCCCCHHHCCCCEEECCHHHHHHHHHCCCCCCCEEEEECCCHHHH
TQRLAAKAGRVVCVELDRGLVQVLHDTVQKAYDNVEVIHGDAGRIDLHKLLGERLAPGQK
HHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCC
AKVVANLPYYITTPLVMRLLEEELPLSHVVVMVQKEVADRMVSPPGSKAYGALSVAVQYY
CEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TEPRIVLRVSRASFMPQPEVDSAVVSLRYRERPPVDAPPEAFFRVVRAAFGQRRKSLVNA
CCCEEEEEEHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LTSLGVEKAAVHAALEAAGIDPGRRGESLSLEEFAAVARTLWQRT
HHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLANPGALKALMAQYGLRPQHRLGQNFLIDGRVLDGIVSAAGLEPTDVVLEIGPGLGTL
CCCCCHHHHHHHHHHHCCCCHHHCCCCEEECCHHHHHHHHHCCCCCCCEEEEECCCHHHH
TQRLAAKAGRVVCVELDRGLVQVLHDTVQKAYDNVEVIHGDAGRIDLHKLLGERLAPGQK
HHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCC
AKVVANLPYYITTPLVMRLLEEELPLSHVVVMVQKEVADRMVSPPGSKAYGALSVAVQYY
CEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TEPRIVLRVSRASFMPQPEVDSAVVSLRYRERPPVDAPPEAFFRVVRAAFGQRRKSLVNA
CCCEEEEEEHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LTSLGVEKAAVHAALEAAGIDPGRRGESLSLEEFAAVARTLWQRT
HHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA