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The map label for this gene is tilS

Identifier: 51894334

GI number: 51894334

Start: 3409891

End: 3411339

Strand: Reverse

Name: tilS

Synonym: STH3199

Alternate gene names: 51894334

Gene position: 3411339-3409891 (Counterclockwise)

Preceding gene: 51894335

Following gene: 51894333

Centisome position: 95.66

GC content: 74.19

Gene sequence:

>1449_bases
GTGCCGCTGCTCGATCGGGTGCGCGAATACGTGGTCCGGCACGGGATGCTCGAGCCGGGCGATCGGGTGATCGTGGCCGT
CTCCGGGGGCCCTGACTCTCTGGCGCTCACGCACGTGCTCTACCGCCTGGCCCCCGCGTGGCGGCTTTCTCTGCACCTTT
TTCATCTAGACCACGGCCTGCGCGGCGAGGCGTCGCGGGCGGATGCCGCCTTCGTCGCCGACCTGGCCCGGGAGCTTTCG
CTGCCGCTGACCACGGTAACGCTCCGCCCCGGCGAGTTGGAGGCCATGCGGGGTTCGCTCGAGGACAACGCCCGCCGCCG
CCGGTACGCGGAGATGGCCCGCCTCGCGGCGGCCATCGGGGCGCAGCGGGCCGCCACCGGCCACAACCGGAACGACCAGG
CGGAAACGGTGCTGATGCGGCTGCTGCGGGGCAGCGGTTCCACCGGCCTTGCCGGCATCCCGCCCGTGCGGCGGGAGGGC
GGCCTGACCATCATCCGGCCGCTGCTGGGCGCCTCGCGGCAGGAGATCCTGGCGTACTGCCGGGCGCACGGGCTCACGCC
CCGCATGGACGCCACCAACCTGCAGGGGGACTTTGAGCGCAACCGGATCCGGCTGGAGATCCTGCCGGCCCTGACGGAGC
GGTTCGGCGACGCGGTGGTGGACAACCTCGCCCAGACGGCGGACCTGCTCCGGGAGGAGGACCGGCTGCTCGCGGAGCTC
ACCCGGGAGGCCTGCGCCCGCTGCGGGTGGCGGGAGACCGGGGAGGCGACCGGCGAGCCGGTGGTCGAGCTGGACGGGAC
GCTCCTGGTGCAGGAGCCTCTGGCCCTGGCCCGGCGGATCGTCCGGATGGCCGTGCAGCGGGTGAGCGGCTCGGCCTACG
GCCCGGGGCTCTCCGCTGTGACCCGGGCCCTGGAGCTGGCCGGCCGGACGGAGGGCAGCCACTGGCTGGACCTGCCGCAG
GGGGTTCGCCTGTCCGTGGCTTACGGCCGCTGCCGGTTCGCGCGGTCCCATCCGCAACTGGGGCCGGGGGCGGAGGCGTT
GGAACAGGTCTGGCCGGTGGCCGTGCCGGGCGAGACGGCGATTCCGGCCCTGGGCGTGACGGTGGCGGCGGAGCTGGTTC
CGCCGTCCGCGATGCCGGCGCGGCTGCCGGACGACGAGATGTGGCTCGACCGGGACCGGCTTCCGGGGCCGCTGGCGGTG
CGGACGCGCCGTCCCGGGGACCGGCTCTGGCCGGCGGGCATGCAGGGTTCCAAGAAGCTTCAGGACATCCTGGTGGATGC
GAAGGTGCCCCGGGACCAGCGGGATGGTCTGCCGCTGCTGGTTGCGGGCGATACTGTAGTCTGGGTGCCCGGGGTGATCC
GGGACCGCCGCTTCCGACCTGACGCCGGGACGCGTTCGGCCGTGCGGGTGACGGTCCGCAGGCGTCCGGAGGGGTCTGGC
GGCGATTGA

Upstream 100 bases:

>100_bases
GGACGGAAAGCTGCACGGAAACCGGAAGGGAATCCCCGGCCGGTGTCGAAACGGGAATCGGCACCTGGGCCGGGGATTGG
CGTGGAGGGGGGGTGACCGG

Downstream 100 bases:

>100_bases
AGCCCTCTGGCGTCGGTTGCTCCGGCGAATTGAATATGGTACACTGAACAAGTATCTTTTGCGGCCATACGGCCGACTCA
ATGGTTGAGCAAGAGTATCT

Product: cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MPLLDRVREYVVRHGMLEPGDRVIVAVSGGPDSLALTHVLYRLAPAWRLSLHLFHLDHGLRGEASRADAAFVADLARELS
LPLTTVTLRPGELEAMRGSLEDNARRRRYAEMARLAAAIGAQRAATGHNRNDQAETVLMRLLRGSGSTGLAGIPPVRREG
GLTIIRPLLGASRQEILAYCRAHGLTPRMDATNLQGDFERNRIRLEILPALTERFGDAVVDNLAQTADLLREEDRLLAEL
TREACARCGWRETGEATGEPVVELDGTLLVQEPLALARRIVRMAVQRVSGSAYGPGLSAVTRALELAGRTEGSHWLDLPQ
GVRLSVAYGRCRFARSHPQLGPGAEALEQVWPVAVPGETAIPALGVTVAAELVPPSAMPARLPDDEMWLDRDRLPGPLAV
RTRRPGDRLWPAGMQGSKKLQDILVDAKVPRDQRDGLPLLVAGDTVVWVPGVIRDRRFRPDAGTRSAVRVTVRRRPEGSG
GD

Sequences:

>Translated_482_residues
MPLLDRVREYVVRHGMLEPGDRVIVAVSGGPDSLALTHVLYRLAPAWRLSLHLFHLDHGLRGEASRADAAFVADLARELS
LPLTTVTLRPGELEAMRGSLEDNARRRRYAEMARLAAAIGAQRAATGHNRNDQAETVLMRLLRGSGSTGLAGIPPVRREG
GLTIIRPLLGASRQEILAYCRAHGLTPRMDATNLQGDFERNRIRLEILPALTERFGDAVVDNLAQTADLLREEDRLLAEL
TREACARCGWRETGEATGEPVVELDGTLLVQEPLALARRIVRMAVQRVSGSAYGPGLSAVTRALELAGRTEGSHWLDLPQ
GVRLSVAYGRCRFARSHPQLGPGAEALEQVWPVAVPGETAIPALGVTVAAELVPPSAMPARLPDDEMWLDRDRLPGPLAV
RTRRPGDRLWPAGMQGSKKLQDILVDAKVPRDQRDGLPLLVAGDTVVWVPGVIRDRRFRPDAGTRSAVRVTVRRRPEGSG
GD
>Mature_481_residues
PLLDRVREYVVRHGMLEPGDRVIVAVSGGPDSLALTHVLYRLAPAWRLSLHLFHLDHGLRGEASRADAAFVADLARELSL
PLTTVTLRPGELEAMRGSLEDNARRRRYAEMARLAAAIGAQRAATGHNRNDQAETVLMRLLRGSGSTGLAGIPPVRREGG
LTIIRPLLGASRQEILAYCRAHGLTPRMDATNLQGDFERNRIRLEILPALTERFGDAVVDNLAQTADLLREEDRLLAELT
REACARCGWRETGEATGEPVVELDGTLLVQEPLALARRIVRMAVQRVSGSAYGPGLSAVTRALELAGRTEGSHWLDLPQG
VRLSVAYGRCRFARSHPQLGPGAEALEQVWPVAVPGETAIPALGVTVAAELVPPSAMPARLPDDEMWLDRDRLPGPLAVR
TRRPGDRLWPAGMQGSKKLQDILVDAKVPRDQRDGLPLLVAGDTVVWVPGVIRDRRFRPDAGTRSAVRVTVRRRPEGSGG
D

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Homo sapiens, GI222537719, Length=179, Percent_Identity=35.195530726257, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1786386, Length=444, Percent_Identity=28.1531531531532, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_SYMTH (Q67JG9)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_077025.1
- ProteinModelPortal:   Q67JG9
- SMR:   Q67JG9
- GeneID:   2982133
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH3199
- NMPDR:   fig|292459.1.peg.3070
- HOGENOM:   HBG668702
- OMA:   YLRTRIR
- BioCyc:   STHE292459:STH3199-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 52557; Mature: 52426

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLDRVREYVVRHGMLEPGDRVIVAVSGGPDSLALTHVLYRLAPAWRLSLHLFHLDHGL
CCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHEEEEEEECCCC
RGEASRADAAFVADLARELSLPLTTVTLRPGELEAMRGSLEDNARRRRYAEMARLAAAIG
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AQRAATGHNRNDQAETVLMRLLRGSGSTGLAGIPPVRREGGLTIIRPLLGASRQEILAYC
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHCCCHHHHHHHH
RAHGLTPRMDATNLQGDFERNRIRLEILPALTERFGDAVVDNLAQTADLLREEDRLLAEL
HHCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TREACARCGWRETGEATGEPVVELDGTLLVQEPLALARRIVRMAVQRVSGSAYGPGLSAV
HHHHHHHCCCCCCCCCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
TRALELAGRTEGSHWLDLPQGVRLSVAYGRCRFARSHPQLGPGAEALEQVWPVAVPGETA
HHHHHHHCCCCCCCEECCCCCCEEEEEHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCC
IPALGVTVAAELVPPSAMPARLPDDEMWLDRDRLPGPLAVRTRRPGDRLWPAGMQGSKKL
CCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHH
QDILVDAKVPRDQRDGLPLLVAGDTVVWVPGVIRDRRFRPDAGTRSAVRVTVRRRPEGSG
HHHHHHCCCCCCCCCCCEEEEECCEEEECCCHHHCCCCCCCCCCCCEEEEEEEECCCCCC
GD
CC
>Mature Secondary Structure 
PLLDRVREYVVRHGMLEPGDRVIVAVSGGPDSLALTHVLYRLAPAWRLSLHLFHLDHGL
CHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHEEEEEEECCCC
RGEASRADAAFVADLARELSLPLTTVTLRPGELEAMRGSLEDNARRRRYAEMARLAAAIG
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AQRAATGHNRNDQAETVLMRLLRGSGSTGLAGIPPVRREGGLTIIRPLLGASRQEILAYC
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHCCCHHHHHHHH
RAHGLTPRMDATNLQGDFERNRIRLEILPALTERFGDAVVDNLAQTADLLREEDRLLAEL
HHCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TREACARCGWRETGEATGEPVVELDGTLLVQEPLALARRIVRMAVQRVSGSAYGPGLSAV
HHHHHHHCCCCCCCCCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
TRALELAGRTEGSHWLDLPQGVRLSVAYGRCRFARSHPQLGPGAEALEQVWPVAVPGETA
HHHHHHHCCCCCCCEECCCCCCEEEEEHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCC
IPALGVTVAAELVPPSAMPARLPDDEMWLDRDRLPGPLAVRTRRPGDRLWPAGMQGSKKL
CCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHH
QDILVDAKVPRDQRDGLPLLVAGDTVVWVPGVIRDRRFRPDAGTRSAVRVTVRRRPEGSG
HHHHHHCCCCCCCCCCCEEEEECCEEEECCCHHHCCCCCCCCCCCCEEEEEEEECCCCCC
GD
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA