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The map label for this gene is dfrA [H]

Identifier: 51894251

GI number: 51894251

Start: 3316303

End: 3316800

Strand: Reverse

Name: dfrA [H]

Synonym: STH3116

Alternate gene names: 51894251

Gene position: 3316800-3316303 (Counterclockwise)

Preceding gene: 51894252

Following gene: 51894250

Centisome position: 93.01

GC content: 70.28

Gene sequence:

>498_bases
ATGGGCAGGAACCGCGCCATCGGCCGGGAGGGCCGCCTGCCCTGGCGGCTGCCGGCGGAGATGCGCCACTTCCGCCGCAC
CACCATGGGCAAGGCGGTGGTGATGGGACGCAGGACCTACGAGTCGATCGGCGGCCCCCTGAAGGGGCGGACCAACATCG
TGCTTACCCGGGACCCGGGCTTCCGGGCGCCGGGATGCGAGGTGCGCCACAGCGTCGCGGAGCTGCTGCAGGACCGCAGG
CCGCTCTATGTCATCGGCGGCGCGGAGGTGTACCGCCAGTTCCTGCCTCATGCGGACGAGATGATCCTCACCCGCATTGA
CCACGACTTCGAAGGGGACGCGTTCTTCCCGGCGTGGTCCGAGGACGAGTGGGAGCTGGTGGGGAGCACGCCCCACCCGC
GGGACGAGGAGAACCCGTACGATTTCGTCGTCGAGCACTACCGCCGGCGGCGCATTCCCGCCGGCGGGGCCGGGCAGACT
GAACCCGAAGGAGACTGA

Upstream 100 bases:

>100_bases
AGATCGACCAGTTCACGATGGACGACATCCAGCTGGTGGACTACAAGCACCACCCGCCGATCCGCGGGGAGCTGTCGACA
TGATCACCCTGGTGGCGGCG

Downstream 100 bases:

>100_bases
GCTCGTGTCTGGACAGATCGAAGGACGGAACCCCGTGCTGGAGGCGCTCCGGTCGGGGCGGACCATCAACCGGATCCTGG
TGGCAAAGGGAGCCCGGGAG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MGRNRAIGREGRLPWRLPAEMRHFRRTTMGKAVVMGRRTYESIGGPLKGRTNIVLTRDPGFRAPGCEVRHSVAELLQDRR
PLYVIGGAEVYRQFLPHADEMILTRIDHDFEGDAFFPAWSEDEWELVGSTPHPRDEENPYDFVVEHYRRRRIPAGGAGQT
EPEGD

Sequences:

>Translated_165_residues
MGRNRAIGREGRLPWRLPAEMRHFRRTTMGKAVVMGRRTYESIGGPLKGRTNIVLTRDPGFRAPGCEVRHSVAELLQDRR
PLYVIGGAEVYRQFLPHADEMILTRIDHDFEGDAFFPAWSEDEWELVGSTPHPRDEENPYDFVVEHYRRRRIPAGGAGQT
EPEGD
>Mature_164_residues
GRNRAIGREGRLPWRLPAEMRHFRRTTMGKAVVMGRRTYESIGGPLKGRTNIVLTRDPGFRAPGCEVRHSVAELLQDRRP
LYVIGGAEVYRQFLPHADEMILTRIDHDFEGDAFFPAWSEDEWELVGSTPHPRDEENPYDFVVEHYRRRRIPAGGAGQTE
PEGD

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=157, Percent_Identity=31.8471337579618, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1786233, Length=153, Percent_Identity=43.7908496732026, Blast_Score=124, Evalue=3e-30,
Organism=Drosophila melanogaster, GI116008026, Length=164, Percent_Identity=30.4878048780488, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24647458, Length=164, Percent_Identity=30.4878048780488, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18941; Mature: 18809

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRNRAIGREGRLPWRLPAEMRHFRRTTMGKAVVMGRRTYESIGGPLKGRTNIVLTRDPG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEECCCC
FRAPGCEVRHSVAELLQDRRPLYVIGGAEVYRQFLPHADEMILTRIDHDFEGDAFFPAWS
CCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCHHHHHHHHCCCCCCCEECCCCC
EDEWELVGSTPHPRDEENPYDFVVEHYRRRRIPAGGAGQTEPEGD
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GRNRAIGREGRLPWRLPAEMRHFRRTTMGKAVVMGRRTYESIGGPLKGRTNIVLTRDPG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEECCCC
FRAPGCEVRHSVAELLQDRRPLYVIGGAEVYRQFLPHADEMILTRIDHDFEGDAFFPAWS
CCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCHHHHHHHHCCCCCCCEECCCCC
EDEWELVGSTPHPRDEENPYDFVVEHYRRRRIPAGGAGQTEPEGD
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]