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The map label for this gene is thyA

Identifier: 51894252

GI number: 51894252

Start: 3316818

End: 3317669

Strand: Reverse

Name: thyA

Synonym: STH3117

Alternate gene names: 51894252

Gene position: 3317669-3316818 (Counterclockwise)

Preceding gene: 51894253

Following gene: 51894251

Centisome position: 93.03

GC content: 66.43

Gene sequence:

>852_bases
TTGAACATCGAGATCCAGTACCTGGAACTCTGCCGGGAGATCCTGGCGGAGAACGAGCAGGAGATCGCCGAGGGCCGCTG
GACGCCCAACCGGACCGGCATGGGGACGGTGGGCGTCTTCGGCCGCATGCTGAAGCACGACTTCAAGGAAGGGTTTCCCG
CCCTGACCACCAAGAAGCTGTTCTTCAACTCCGTGAAGGCCGAGTTGCTCTGGTTCCTCGCGGGCCGGACCGACCTGGAG
TTCCTGCACGAGCGGGGATGCCACATCTGGGACAAGGACGCGGCCCGCAACGGCGGGAAGCTCGGGCCGATCTACGGCAA
GCAGTGGCGGGCGTGGGAGGGCGCGGACGGCAGGACCTACGACCAGATCGCCTGGGTCATCCAGCAGATCCGGGAGATCG
CCGCCGACCCGGAGCGGCCCCATCCCAACTCCAGGCGGCTGGTCGTCAGCCCCTGGAACGTCGCCGACCTGGAGGAGATG
GCCCTGCCGCCCTGCCACGGGCCGTTCCAGTTCCACGTGAAGGACGGCAGGCTGCACCTGGCCGTGTGGCAGCGCAGCGC
CGACCTGTTCCTGGGAGTGCCCTTCAACATCGCCAGCTACTCGCTGCTGCTGCACATGGTGGCGCAGGTGACCGGGCTGG
CGGTGGGCACGTACACGCATTACCTGAACGACGTGCACATCTACCGCAACCTCCTGGACCAGGTACGGCTGCAGCTCACG
CGGGAGCCGTACCCGCTGCCCAGGCTCTGGCTGAACCCCGAGGTCAGGGAGATCGACCAGTTCACGATGGACGACATCCA
GCTGGTGGACTACAAGCACCACCCGCCGATCCGCGGGGAGCTGTCGACATGA

Upstream 100 bases:

>100_bases
CATGTCGGCGCGCCTGCGCCTGGTTTTCGTGACAGGAAGAAGGATCCCGGGGCAGAATTAGTTCTAGTTAATTAAGGTTG
CTGGCCCGGGGGGGTCGGGA

Downstream 100 bases:

>100_bases
TCACCCTGGTGGCGGCGATGGGCAGGAACCGCGCCATCGGCCGGGAGGGCCGCCTGCCCTGGCGGCTGCCGGCGGAGATG
CGCCACTTCCGCCGCACCAC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MNIEIQYLELCREILAENEQEIAEGRWTPNRTGMGTVGVFGRMLKHDFKEGFPALTTKKLFFNSVKAELLWFLAGRTDLE
FLHERGCHIWDKDAARNGGKLGPIYGKQWRAWEGADGRTYDQIAWVIQQIREIAADPERPHPNSRRLVVSPWNVADLEEM
ALPPCHGPFQFHVKDGRLHLAVWQRSADLFLGVPFNIASYSLLLHMVAQVTGLAVGTYTHYLNDVHIYRNLLDQVRLQLT
REPYPLPRLWLNPEVREIDQFTMDDIQLVDYKHHPPIRGELST

Sequences:

>Translated_283_residues
MNIEIQYLELCREILAENEQEIAEGRWTPNRTGMGTVGVFGRMLKHDFKEGFPALTTKKLFFNSVKAELLWFLAGRTDLE
FLHERGCHIWDKDAARNGGKLGPIYGKQWRAWEGADGRTYDQIAWVIQQIREIAADPERPHPNSRRLVVSPWNVADLEEM
ALPPCHGPFQFHVKDGRLHLAVWQRSADLFLGVPFNIASYSLLLHMVAQVTGLAVGTYTHYLNDVHIYRNLLDQVRLQLT
REPYPLPRLWLNPEVREIDQFTMDDIQLVDYKHHPPIRGELST
>Mature_283_residues
MNIEIQYLELCREILAENEQEIAEGRWTPNRTGMGTVGVFGRMLKHDFKEGFPALTTKKLFFNSVKAELLWFLAGRTDLE
FLHERGCHIWDKDAARNGGKLGPIYGKQWRAWEGADGRTYDQIAWVIQQIREIAADPERPHPNSRRLVVSPWNVADLEEM
ALPPCHGPFQFHVKDGRLHLAVWQRSADLFLGVPFNIASYSLLLHMVAQVTGLAVGTYTHYLNDVHIYRNLLDQVRLQLT
REPYPLPRLWLNPEVREIDQFTMDDIQLVDYKHHPPIRGELST

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=300, Percent_Identity=39.6666666666667, Blast_Score=216, Evalue=2e-56,
Organism=Escherichia coli, GI1789191, Length=277, Percent_Identity=45.4873646209386, Blast_Score=254, Evalue=6e-69,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=43.4628975265018, Blast_Score=236, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6324648, Length=302, Percent_Identity=34.4370860927152, Blast_Score=169, Evalue=5e-43,
Organism=Drosophila melanogaster, GI17137556, Length=294, Percent_Identity=42.5170068027211, Blast_Score=244, Evalue=4e-65,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_SYMTH (Q67JQ1)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076943.1
- ProteinModelPortal:   Q67JQ1
- SMR:   Q67JQ1
- GeneID:   2979321
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH3117
- NMPDR:   fig|292459.1.peg.2988
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BioCyc:   STHE292459:STH3117-MONOMER
- BRENDA:   2.1.1.45
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 32761; Mature: 32761

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE; PS00226 IF

Important sites: ACT_SITE 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEIQYLELCREILAENEQEIAEGRWTPNRTGMGTVGVFGRMLKHDFKEGFPALTTKKL
CCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
FFNSVKAELLWFLAGRTDLEFLHERGCHIWDKDAARNGGKLGPIYGKQWRAWEGADGRTY
HHHHHHHHHHHHHCCCCHHHHHHHCCCCEECCHHHCCCCCCCCCCCCCCCCCCCCCCCHH
DQIAWVIQQIREIAADPERPHPNSRRLVVSPWNVADLEEMALPPCHGPFQFHVKDGRLHL
HHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCEEEEEECCEEEE
AVWQRSADLFLGVPFNIASYSLLLHMVAQVTGLAVGTYTHYLNDVHIYRNLLDQVRLQLT
EEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
REPYPLPRLWLNPEVREIDQFTMDDIQLVDYKHHPPIRGELST
CCCCCCCCEECCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNIEIQYLELCREILAENEQEIAEGRWTPNRTGMGTVGVFGRMLKHDFKEGFPALTTKKL
CCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
FFNSVKAELLWFLAGRTDLEFLHERGCHIWDKDAARNGGKLGPIYGKQWRAWEGADGRTY
HHHHHHHHHHHHHCCCCHHHHHHHCCCCEECCHHHCCCCCCCCCCCCCCCCCCCCCCCHH
DQIAWVIQQIREIAADPERPHPNSRRLVVSPWNVADLEEMALPPCHGPFQFHVKDGRLHL
HHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCEEEEEECCEEEE
AVWQRSADLFLGVPFNIASYSLLLHMVAQVTGLAVGTYTHYLNDVHIYRNLLDQVRLQLT
EEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
REPYPLPRLWLNPEVREIDQFTMDDIQLVDYKHHPPIRGELST
CCCCCCCCEECCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA