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The map label for this gene is purN [H]

Identifier: 51893990

GI number: 51893990

Start: 3074052

End: 3074678

Strand: Reverse

Name: purN [H]

Synonym: STH2852

Alternate gene names: 51893990

Gene position: 3074678-3074052 (Counterclockwise)

Preceding gene: 51893991

Following gene: 51893989

Centisome position: 86.22

GC content: 73.37

Gene sequence:

>627_bases
ATGATCCGCATCGGCGTGTTGATCTCCGGCTCGGGCACCAACCTGCAGGCGATCCTGGACGGCTGCCGGGAGGGGCGCAT
CCCCGGCCGGGTGGCGGTGGTCATCTCCGACCGGGCCGATGCCTACGGGCTGGAGCGGGCCCGCCGGGCCGGGGTCGATG
CCCTGCACATGGACCCGGCCGCGTACCCGAGCCGGACAGCCTTTGACGCGGCGCTGGCGGAGCGGCTGCAGGCCTACGGG
GTCGATCTGGTCTGCCTCGCGGGGTACATGCGGCTGGTCCGCGGGCCGATGCTCACGGCGTTCCCGAACCGCATCCTCAA
CATCCACCCGTCGCTGCTGCCGGCCTTCCCGGGGCTGGAGGCCCAGCGGCAGGCCCTGGAGCACGGGGTGAAGGTGGCGG
GTTGCACGGTCCACTTCGTCACGGCGGGCGTGGACGAGGGGCCCATCATCCTGCAGGCCGCCGTGCCGGTGCTGGAGGGG
GACACGGTGGAGGACCTGCGCCGGCGCATCCTGGCCGAGGAGCACCGCATCTACCCCGAGGCGATCCGGCTGTTCGCGGA
GGGGCGGCTGGTGATCGAGGGGCGCCGGGTGCGGATCCTCGACCGGGCGGAGGCTCCCCGCGGGTAA

Upstream 100 bases:

>100_bases
AGGCGGTTGCGGCCGCCGCGCGGGAGTTGGGCGAGGAGGCGTGGGTTGTGGGTGAGGTCGCGGCCGCCGAGCCCGGCGGT
CCGAGGGTGGTGGTGCGGCG

Downstream 100 bases:

>100_bases
CCCGGGTCTCCGTTTGGGCAGGCCCCCCGGGCGCCTGGTGCGGCAGGAGAACCCGGGCCCGTGCGGGTACACTCTCCGGC
GGGGGCTCGCTCCCGCATCG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MIRIGVLISGSGTNLQAILDGCREGRIPGRVAVVISDRADAYGLERARRAGVDALHMDPAAYPSRTAFDAALAERLQAYG
VDLVCLAGYMRLVRGPMLTAFPNRILNIHPSLLPAFPGLEAQRQALEHGVKVAGCTVHFVTAGVDEGPIILQAAVPVLEG
DTVEDLRRRILAEEHRIYPEAIRLFAEGRLVIEGRRVRILDRAEAPRG

Sequences:

>Translated_208_residues
MIRIGVLISGSGTNLQAILDGCREGRIPGRVAVVISDRADAYGLERARRAGVDALHMDPAAYPSRTAFDAALAERLQAYG
VDLVCLAGYMRLVRGPMLTAFPNRILNIHPSLLPAFPGLEAQRQALEHGVKVAGCTVHFVTAGVDEGPIILQAAVPVLEG
DTVEDLRRRILAEEHRIYPEAIRLFAEGRLVIEGRRVRILDRAEAPRG
>Mature_208_residues
MIRIGVLISGSGTNLQAILDGCREGRIPGRVAVVISDRADAYGLERARRAGVDALHMDPAAYPSRTAFDAALAERLQAYG
VDLVCLAGYMRLVRGPMLTAFPNRILNIHPSLLPAFPGLEAQRQALEHGVKVAGCTVHFVTAGVDEGPIILQAAVPVLEG
DTVEDLRRRILAEEHRIYPEAIRLFAEGRLVIEGRRVRILDRAEAPRG

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=186, Percent_Identity=48.9247311827957, Blast_Score=187, Evalue=4e-48,
Organism=Homo sapiens, GI209869995, Length=186, Percent_Identity=48.9247311827957, Blast_Score=187, Evalue=4e-48,
Organism=Homo sapiens, GI209869993, Length=186, Percent_Identity=48.9247311827957, Blast_Score=187, Evalue=4e-48,
Organism=Escherichia coli, GI1788846, Length=189, Percent_Identity=49.7354497354497, Blast_Score=196, Evalue=7e-52,
Organism=Escherichia coli, GI1787483, Length=137, Percent_Identity=39.4160583941606, Blast_Score=100, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=41.4364640883978, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=31.3131313131313, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24582400, Length=193, Percent_Identity=46.6321243523316, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22603; Mature: 22603

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIGVLISGSGTNLQAILDGCREGRIPGRVAVVISDRADAYGLERARRAGVDALHMDPA
CEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEECCCC
AYPSRTAFDAALAERLQAYGVDLVCLAGYMRLVRGPMLTAFPNRILNIHPSLLPAFPGLE
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHCCHHHEECCHHHHCCCCCCH
AQRQALEHGVKVAGCTVHFVTAGVDEGPIILQAAVPVLEGDTVEDLRRRILAEEHRIYPE
HHHHHHHCCCEEEEEEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHH
AIRLFAEGRLVIEGRRVRILDRAEAPRG
HHHHHHCCEEEEECCEEEEEECCCCCCC
>Mature Secondary Structure
MIRIGVLISGSGTNLQAILDGCREGRIPGRVAVVISDRADAYGLERARRAGVDALHMDPA
CEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEECCCC
AYPSRTAFDAALAERLQAYGVDLVCLAGYMRLVRGPMLTAFPNRILNIHPSLLPAFPGLE
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHCCHHHEECCHHHHCCCCCCH
AQRQALEHGVKVAGCTVHFVTAGVDEGPIILQAAVPVLEGDTVEDLRRRILAEEHRIYPE
HHHHHHHCCCEEEEEEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHH
AIRLFAEGRLVIEGRRVRILDRAEAPRG
HHHHHHCCEEEEECCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]