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The map label for this gene is fdnH [C]

Identifier: 51893738

GI number: 51893738

Start: 2789500

End: 2790291

Strand: Reverse

Name: fdnH [C]

Synonym: STH2600

Alternate gene names: 51893738

Gene position: 2790291-2789500 (Counterclockwise)

Preceding gene: 51893739

Following gene: 51893737

Centisome position: 78.24

GC content: 69.32

Gene sequence:

>792_bases
ATGACCTACGGGATGCTCATCGACCTGTCCAAGTGCATGGGCTGCCGGGGCTGCCAGGTGGCCTGCAAGCAGTGGAACGA
CCTCCCGGGTGAGGACACGAGCGTGGGGCCCGACTTCACCAACCCGCAGAAGCGCTCGGCCTACACCTGGACCACCATCG
ACTTCGTGACCGCCGAGGTGAACGGCCGCACCGTGGCCACCTTCACCAAGTCCGGCTGCATGCACTGCCTGGAGCCGGCC
TGCGAGTCGGTCTGCATCGCCGGCGCCATCAAGCGGCAGGCGAACGGCGCGGTCACCATCGACCACGACAAGTGCATCGG
CTGCCGCTACTGCCAGCTGGGCTGCCCCTTCGGTGTGCCCAAGTACGAGTACGACAAGCCGGTGCCCCGCATGCAGAAGT
GCACCCTGTGCTACGACCGCATCTCGGAGGGCCTGGAGCCGGCCTGCGCGGCCACCTGCCCCAGCGGCGCCATCCTCTTC
GGCGAGCGGGAAGAGCTGCTGAAGCTCGCCAGGCAGCGCATCGCCGAGGGGGGCGGCCGGTACATCGACCACGTGTACGG
CGAGCACGAGGCGGGCGGCACCGCCGTCCTGTACATCTCCCCCGTGCCGCTGGACCTGACCGCCCTGAACACCGACGTCA
CGACCGAGTCGGTGCCCGGCTTCACCTGGAAGGCCATGCGGCAGCTGCCGGTCCTGGTGGGCAGCGTCGCAGCCCTGTCG
CTGGGACTCATGGCCTACTCGCGGCGGCGCGCCCAGCTTGAGGCGCTGGCGAAGGGGGGTGGCGAGCAGTGA

Upstream 100 bases:

>100_bases
CCGCACGTGGGCGACGCCAACACCCAGATCCCCGAGTACAAGGCGTTCCTGTGCAACGTGAAGAAGGCCGGCGGCTCCGG
CCCGGTAAGGGGGTAAGCTC

Downstream 100 bases:

>100_bases
GCACCATGAACGCGACGCACAAGCCGACCAGGCTTCTGCGGATGGAGTGGTGGCGCTGGCGGATGTCGCCGGGCAAGGGG
CTGTTGCTCGCCCTCTTCGG

Product: formate dehydrogenase beta subunit

Products: CO2; NADH

Alternate protein names: HMC operon ORF 2 [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTYGMLIDLSKCMGCRGCQVACKQWNDLPGEDTSVGPDFTNPQKRSAYTWTTIDFVTAEVNGRTVATFTKSGCMHCLEPA
CESVCIAGAIKRQANGAVTIDHDKCIGCRYCQLGCPFGVPKYEYDKPVPRMQKCTLCYDRISEGLEPACAATCPSGAILF
GEREELLKLARQRIAEGGGRYIDHVYGEHEAGGTAVLYISPVPLDLTALNTDVTTESVPGFTWKAMRQLPVLVGSVAALS
LGLMAYSRRRAQLEALAKGGGEQ

Sequences:

>Translated_263_residues
MTYGMLIDLSKCMGCRGCQVACKQWNDLPGEDTSVGPDFTNPQKRSAYTWTTIDFVTAEVNGRTVATFTKSGCMHCLEPA
CESVCIAGAIKRQANGAVTIDHDKCIGCRYCQLGCPFGVPKYEYDKPVPRMQKCTLCYDRISEGLEPACAATCPSGAILF
GEREELLKLARQRIAEGGGRYIDHVYGEHEAGGTAVLYISPVPLDLTALNTDVTTESVPGFTWKAMRQLPVLVGSVAALS
LGLMAYSRRRAQLEALAKGGGEQ
>Mature_262_residues
TYGMLIDLSKCMGCRGCQVACKQWNDLPGEDTSVGPDFTNPQKRSAYTWTTIDFVTAEVNGRTVATFTKSGCMHCLEPAC
ESVCIAGAIKRQANGAVTIDHDKCIGCRYCQLGCPFGVPKYEYDKPVPRMQKCTLCYDRISEGLEPACAATCPSGAILFG
EREELLKLARQRIAEGGGRYIDHVYGEHEAGGTAVLYISPVPLDLTALNTDVTTESVPGFTWKAMRQLPVLVGSVAALSL
GLMAYSRRRAQLEALAKGGGEQ

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=197, Percent_Identity=47.2081218274112, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1790326, Length=250, Percent_Identity=39.6, Blast_Score=167, Evalue=5e-43,
Organism=Escherichia coli, GI1789370, Length=291, Percent_Identity=34.7079037800687, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1787872, Length=168, Percent_Identity=40.4761904761905, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1787122, Length=170, Percent_Identity=40, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI226510944, Length=166, Percent_Identity=29.5180722891566, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI2367345, Length=180, Percent_Identity=31.6666666666667, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI2367245, Length=178, Percent_Identity=29.2134831460674, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789067, Length=187, Percent_Identity=29.9465240641711, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787478, Length=91, Percent_Identity=41.7582417582418, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787963, Length=163, Percent_Identity=28.2208588957055, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787740, Length=91, Percent_Identity=40.6593406593407, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082180, Length=181, Percent_Identity=29.2817679558011, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082179, Length=180, Percent_Identity=29.4444444444444, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: 1.2.1.2

Molecular weight: Translated: 28451; Mature: 28320

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
6.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYGMLIDLSKCMGCRGCQVACKQWNDLPGEDTSVGPDFTNPQKRSAYTWTTIDFVTAEV
CCCCCEEEHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEC
NGRTVATFTKSGCMHCLEPACESVCIAGAIKRQANGAVTIDHDKCIGCRYCQLGCPFGVP
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEECCCCCCCC
KYEYDKPVPRMQKCTLCYDRISEGLEPACAATCPSGAILFGEREELLKLARQRIAEGGGR
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCC
YIDHVYGEHEAGGTAVLYISPVPLDLTALNTDVTTESVPGFTWKAMRQLPVLVGSVAALS
CHHHHCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGLMAYSRRRAQLEALAKGGGEQ
HHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TYGMLIDLSKCMGCRGCQVACKQWNDLPGEDTSVGPDFTNPQKRSAYTWTTIDFVTAEV
CCCCEEEHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEC
NGRTVATFTKSGCMHCLEPACESVCIAGAIKRQANGAVTIDHDKCIGCRYCQLGCPFGVP
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEECCCCCCCC
KYEYDKPVPRMQKCTLCYDRISEGLEPACAATCPSGAILFGEREELLKLARQRIAEGGGR
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCC
YIDHVYGEHEAGGTAVLYISPVPLDLTALNTDVTTESVPGFTWKAMRQLPVLVGSVAALS
CHHHHCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGLMAYSRRRAQLEALAKGGGEQ
HHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]