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The map label for this gene is cwlH [H]

Identifier: 51893731

GI number: 51893731

Start: 2783060

End: 2783737

Strand: Reverse

Name: cwlH [H]

Synonym: STH2593

Alternate gene names: 51893731

Gene position: 2783737-2783060 (Counterclockwise)

Preceding gene: 51893732

Following gene: 51893729

Centisome position: 78.06

GC content: 71.09

Gene sequence:

>678_bases
GTGATCCCCATCATCCCCGACATGCTGCCGGAGGACCACCCGCTGGAGCGGCTTCCCATGACCCCGCGCTATATCACCAT
CCACGACACCGGCAACCCGGCGCCCGGCGCCAACGCCGCGATGCACGGCCGCTATATCCGCAGCCAGGCGGCCATCGAGC
GCGAGGTGATGTGGCACTTCACCGTCGACGACCGGGAGATCCGGCAGCACCTGCCGCTGGACCGCAACGGCTGGCACGCC
GGCGACGGCTGGTACGGCACCGGCAACCGGCAGTCCATTGCCATTGAGATCTGCGAGAACGCCGACGGCAACCGGGCCGC
CGCCGAGGCCAACGCCGCCGCCCTGGTGGCCTACCTCATTCAGACCGTGCCCACGCTCCTGCCCTTCCCCGAATGCGTGG
TGCAGCACAACCGCTGGTCCGGAAAAGACTGCCCCCGGGTCCTTCGAGGCAGACCCGGGGGATGGGAGGCGTTTCTGAGC
AGCGTGGCAGCGAACTTGAACGACGGACGGAGGCCGATCCCGGTGCGTGACCCGGCGGGGCGGCTGCTTCTCACCGGCTG
GCTGGAGGACGGCACCACCGTCGTGCCCCTCAGGGCGCTGGCAGAGGCCCTGGGGCTGGTGGTGGAGTGGGACGCCGCAG
CCGCCGAGGCCCGGCTGCGCTGGCCGGGGGGCGCGTAG

Upstream 100 bases:

>100_bases
CCAGGTGTCGGACTGACCCGTCACGCCTCCATGTCACACCGTCCTCCTAGTTTACATATTGTGTCCTGACCCGGACGGCG
CTGAGAGGAGGCGACGCCAG

Downstream 100 bases:

>100_bases
TTCGGGCGTCTGCGCCGGCGTCGGGCGTGCGTCTGCACCGGTCGCCCAGCCGCTACCCGTCCTCACCGGAGCAGTTCCCG
CAGCCCCTCCCAGTCGCCGG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MIPIIPDMLPEDHPLERLPMTPRYITIHDTGNPAPGANAAMHGRYIRSQAAIEREVMWHFTVDDREIRQHLPLDRNGWHA
GDGWYGTGNRQSIAIEICENADGNRAAAEANAAALVAYLIQTVPTLLPFPECVVQHNRWSGKDCPRVLRGRPGGWEAFLS
SVAANLNDGRRPIPVRDPAGRLLLTGWLEDGTTVVPLRALAEALGLVVEWDAAAAEARLRWPGGA

Sequences:

>Translated_225_residues
MIPIIPDMLPEDHPLERLPMTPRYITIHDTGNPAPGANAAMHGRYIRSQAAIEREVMWHFTVDDREIRQHLPLDRNGWHA
GDGWYGTGNRQSIAIEICENADGNRAAAEANAAALVAYLIQTVPTLLPFPECVVQHNRWSGKDCPRVLRGRPGGWEAFLS
SVAANLNDGRRPIPVRDPAGRLLLTGWLEDGTTVVPLRALAEALGLVVEWDAAAAEARLRWPGGA
>Mature_225_residues
MIPIIPDMLPEDHPLERLPMTPRYITIHDTGNPAPGANAAMHGRYIRSQAAIEREVMWHFTVDDREIRQHLPLDRNGWHA
GDGWYGTGNRQSIAIEICENADGNRAAAEANAAALVAYLIQTVPTLLPFPECVVQHNRWSGKDCPRVLRGRPGGWEAFLS
SVAANLNDGRRPIPVRDPAGRLLLTGWLEDGTTVVPLRALAEALGLVVEWDAAAAEARLRWPGGA

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 24722; Mature: 24722

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIIPDMLPEDHPLERLPMTPRYITIHDTGNPAPGANAAMHGRYIRSQAAIEREVMWHF
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEE
TVDDREIRQHLPLDRNGWHAGDGWYGTGNRQSIAIEICENADGNRAAAEANAAALVAYLI
ECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
QTVPTLLPFPECVVQHNRWSGKDCPRVLRGRPGGWEAFLSSVAANLNDGRRPIPVRDPAG
HHHHHHCCCHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
RLLLTGWLEDGTTVVPLRALAEALGLVVEWDAAAAEARLRWPGGA
CEEEEEECCCCCEEEHHHHHHHHHCEEEEECCHHCCCEECCCCCC
>Mature Secondary Structure
MIPIIPDMLPEDHPLERLPMTPRYITIHDTGNPAPGANAAMHGRYIRSQAAIEREVMWHF
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEE
TVDDREIRQHLPLDRNGWHAGDGWYGTGNRQSIAIEICENADGNRAAAEANAAALVAYLI
ECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
QTVPTLLPFPECVVQHNRWSGKDCPRVLRGRPGGWEAFLSSVAANLNDGRRPIPVRDPAG
HHHHHHCCCHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
RLLLTGWLEDGTTVVPLRALAEALGLVVEWDAAAAEARLRWPGGA
CEEEEEECCCCCEEEHHHHHHHHHCEEEEECCHHCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]