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The map label for this gene is pcrA [H]

Identifier: 51893634

GI number: 51893634

Start: 2678288

End: 2680303

Strand: Reverse

Name: pcrA [H]

Synonym: STH2496

Alternate gene names: 51893634

Gene position: 2680303-2678288 (Counterclockwise)

Preceding gene: 51893635

Following gene: 51893633

Centisome position: 75.16

GC content: 70.93

Gene sequence:

>2016_bases
TTGTCCGACCTCGAAGCGATCGTTGAAGGTTTGAACCCAGCGCAGAGGGCGGCCGCCCTGCACCAGGACGGCCCCCTTCT
TGTTATCGCCGGCGCCGGGGCCGGCAAGACCCGGACGGCCACCCACCGGCTGGCCTGTCTTCTGGCCAGGGGGGTCCCGC
CGGAGGCGGTCATGTGCATCACATTCACCAACAAGGCGGCCCGGGAGATGCGGGACCGGGCGGTGGCCCTGGTGGGTGCG
GACGCCCGGCGGGTGATGATCCGCACCTTCCACTCCGCCGCCATGGTGCTCCTCCGGCAGTACATCGGCGACTTCCCGGA
GAGCGGCCGGACGCCTTCCTTCACCGTCGCGGACCCCACGATTCAGCTGGCGCTGCTGAAGGAGGCCATCGCCGAGCGCA
ACTTCGACCTGAAGGCCAACCGGCCGGAGTCGTTCCTCTGGCGCATCGGCCGCTACAAGAACGAGCTGGCCGACCCCGAG
TCGCTGCTCGCCCGGCAGCCCTCCCACGACCTCATGGACTGGTCCCGGGTGCTGGAGATGATCCGCAGCACCGACCGGTA
CGTGAACCGGCTGACGGCGGAGATCTGGAAGCGCTACGAGGAGAAGCTCCGGCGCAACGACATGGTCGACTTCGACGACC
TGATCAACCTGTTCGTCCAGATGCTCCGGGAGAAGCCGGACATTCGCGCGGACCTGCAGCGCCGCTTCACCTACCTGCAG
GTGGACGAGTTCCAGGACACCAACGTCGCCCAGCTCCACATGGTGAAGCTGCTGGCCGGTAAGCGGCAGAACGTGATGGC
GGTGGGCGACGACGCGCAGTCCATCTACTCCTGGCGGTCGGCCGACATCCGCTGCATCCTGGAGTTCGAGCGCCACTTCC
CGGGGGCGAGGACGATCCTGCTGGAGCAGAACTACCGCTCCACCGGGGCCATCATCCGCGCCGCCAACCGGCTGATCGCG
CACAACGCGGGCCAGAGGCCCAAGCGGCTCTTCACCGCCGCGCCCGAGGGGCGGCCCGTGGTGGCCCACGAGGCCGAGAC
GGAGGTGGACGAGGCGAAGTGGGTCGTGCAGCGCATCCGGGCGGGAGCGGAGGCCGGCCGGCGCTGGGGCGACTTCGCCA
TCCTGTACCGCACCACCGCCCAGGGGCGCCCCTTCGAGGAGGAGCTGCGGGAGGCGTCGATCCCCTACCAGGTGGTGGGG
GGCCCCCGCTTCTACGACCGGCGGGAGGTGCAGGACTCGCTGGCCTATCTGCGGCTGGTGCACAACCCCCGGGACTCGGT
CAGCCTGGAGCGGGTGCTGAACGCCCCGCGCCGGGGCATCGGGGAGCGGGGGCTGCAGCGGCTGCTGGCCCTCGCCGCGC
AGCGGGGGGTGGACCTGATCGCGGCGCTGAGGGTCGCAGCCGACGAAGGGGTCCTGCAGGACGAGGCCGCCGCGGGCGCG
CGTCAGCTCCACGACCACCTGCGCCGGGCCCGGGAAGCCCTGGAGCGGCAGCCAGGCCGGTTCGCGGCGGCGATGGAGCG
GCTGCTTCGGGAATCGGGCTACCTGGCGTACCTGCAGGAGGAGGACCGGCGCAAGGAAGAGCGCCGGTCCGAGGTGGTGG
AGAGCCTGATCGCCGCCATGCACGAGTTCGAGCGCCGCTCGCCGGATGGCGGGCTCGGGGCGTACCTGGACCACCTGGCC
CTGTTGGAAGGGCAGGAGGACGAGAGCGGCGGCGACGTGGTGAAGCTGCAGACGATCCACTCGGCCAAGGGGCTGGAGTA
CCCGGTGGTCTTCGTCGTGGGCATGGAGCAGGGGCTGCTGCCGTACCAGCGGGCCCTGGAGGAGGGCAACCTGGAGGAGG
AGCGGCGGCTCTGCTACGTGGCCATCACCCGGGCCAGGGAGGAGCTGTACCTGACCGGCGCCCGGGTGCGCTGGCAGCGG
GGGGAGATGGTGCTCACCACCCCGTCGCAGTTCCTTTCGGAGGCGCTGGGCCCGGGGCCGGCCCAGCCGCTCTGGGTGCC
CGCTTCCGCGGACTAG

Upstream 100 bases:

>100_bases
GGCTGGCTGACCCACGTGCCCGACTGAGAAATGTCAAGTCTCCCCTCGCCTGCCGGGACTTGCTATAATGCCCCCTGGCA
GACAGGGGGGCAGACGAAGC

Downstream 100 bases:

>100_bases
CGGCTCCTCCGGGCCGACCGGAGGGCCGACGGAGGGCAGACAGAGGTGGCCCTGCGCCCGCCCGTATGGTACACTGTTGC
TGTTTATTGGCAAGACAACG

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MSDLEAIVEGLNPAQRAAALHQDGPLLVIAGAGAGKTRTATHRLACLLARGVPPEAVMCITFTNKAAREMRDRAVALVGA
DARRVMIRTFHSAAMVLLRQYIGDFPESGRTPSFTVADPTIQLALLKEAIAERNFDLKANRPESFLWRIGRYKNELADPE
SLLARQPSHDLMDWSRVLEMIRSTDRYVNRLTAEIWKRYEEKLRRNDMVDFDDLINLFVQMLREKPDIRADLQRRFTYLQ
VDEFQDTNVAQLHMVKLLAGKRQNVMAVGDDAQSIYSWRSADIRCILEFERHFPGARTILLEQNYRSTGAIIRAANRLIA
HNAGQRPKRLFTAAPEGRPVVAHEAETEVDEAKWVVQRIRAGAEAGRRWGDFAILYRTTAQGRPFEEELREASIPYQVVG
GPRFYDRREVQDSLAYLRLVHNPRDSVSLERVLNAPRRGIGERGLQRLLALAAQRGVDLIAALRVAADEGVLQDEAAAGA
RQLHDHLRRAREALERQPGRFAAAMERLLRESGYLAYLQEEDRRKEERRSEVVESLIAAMHEFERRSPDGGLGAYLDHLA
LLEGQEDESGGDVVKLQTIHSAKGLEYPVVFVVGMEQGLLPYQRALEEGNLEEERRLCYVAITRAREELYLTGARVRWQR
GEMVLTTPSQFLSEALGPGPAQPLWVPASAD

Sequences:

>Translated_671_residues
MSDLEAIVEGLNPAQRAAALHQDGPLLVIAGAGAGKTRTATHRLACLLARGVPPEAVMCITFTNKAAREMRDRAVALVGA
DARRVMIRTFHSAAMVLLRQYIGDFPESGRTPSFTVADPTIQLALLKEAIAERNFDLKANRPESFLWRIGRYKNELADPE
SLLARQPSHDLMDWSRVLEMIRSTDRYVNRLTAEIWKRYEEKLRRNDMVDFDDLINLFVQMLREKPDIRADLQRRFTYLQ
VDEFQDTNVAQLHMVKLLAGKRQNVMAVGDDAQSIYSWRSADIRCILEFERHFPGARTILLEQNYRSTGAIIRAANRLIA
HNAGQRPKRLFTAAPEGRPVVAHEAETEVDEAKWVVQRIRAGAEAGRRWGDFAILYRTTAQGRPFEEELREASIPYQVVG
GPRFYDRREVQDSLAYLRLVHNPRDSVSLERVLNAPRRGIGERGLQRLLALAAQRGVDLIAALRVAADEGVLQDEAAAGA
RQLHDHLRRAREALERQPGRFAAAMERLLRESGYLAYLQEEDRRKEERRSEVVESLIAAMHEFERRSPDGGLGAYLDHLA
LLEGQEDESGGDVVKLQTIHSAKGLEYPVVFVVGMEQGLLPYQRALEEGNLEEERRLCYVAITRAREELYLTGARVRWQR
GEMVLTTPSQFLSEALGPGPAQPLWVPASAD
>Mature_670_residues
SDLEAIVEGLNPAQRAAALHQDGPLLVIAGAGAGKTRTATHRLACLLARGVPPEAVMCITFTNKAAREMRDRAVALVGAD
ARRVMIRTFHSAAMVLLRQYIGDFPESGRTPSFTVADPTIQLALLKEAIAERNFDLKANRPESFLWRIGRYKNELADPES
LLARQPSHDLMDWSRVLEMIRSTDRYVNRLTAEIWKRYEEKLRRNDMVDFDDLINLFVQMLREKPDIRADLQRRFTYLQV
DEFQDTNVAQLHMVKLLAGKRQNVMAVGDDAQSIYSWRSADIRCILEFERHFPGARTILLEQNYRSTGAIIRAANRLIAH
NAGQRPKRLFTAAPEGRPVVAHEAETEVDEAKWVVQRIRAGAEAGRRWGDFAILYRTTAQGRPFEEELREASIPYQVVGG
PRFYDRREVQDSLAYLRLVHNPRDSVSLERVLNAPRRGIGERGLQRLLALAAQRGVDLIAALRVAADEGVLQDEAAAGAR
QLHDHLRRAREALERQPGRFAAAMERLLRESGYLAYLQEEDRRKEERRSEVVESLIAAMHEFERRSPDGGLGAYLDHLAL
LEGQEDESGGDVVKLQTIHSAKGLEYPVVFVVGMEQGLLPYQRALEEGNLEEERRLCYVAITRAREELYLTGARVRWQRG
EMVLTTPSQFLSEALGPGPAQPLWVPASAD

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=659, Percent_Identity=39.6054628224583, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI48994965, Length=660, Percent_Identity=37.4242424242424, Blast_Score=355, Evalue=6e-99,
Organism=Escherichia coli, GI1787196, Length=363, Percent_Identity=29.7520661157025, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1789183, Length=525, Percent_Identity=23.2380952380952, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 75844; Mature: 75713

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLEAIVEGLNPAQRAAALHQDGPLLVIAGAGAGKTRTATHRLACLLARGVPPEAVMCI
CCHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEE
TFTNKAAREMRDRAVALVGADARRVMIRTFHSAAMVLLRQYIGDFPESGRTPSFTVADPT
EECCHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCH
IQLALLKEAIAERNFDLKANRPESFLWRIGRYKNELADPESLLARQPSHDLMDWSRVLEM
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHH
IRSTDRYVNRLTAEIWKRYEEKLRRNDMVDFDDLINLFVQMLREKPDIRADLQRRFTYLQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHEEE
VDEFQDTNVAQLHMVKLLAGKRQNVMAVGDDAQSIYSWRSADIRCILEFERHFPGARTIL
EECCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCEEEEEHHHHCCCCEEEE
LEQNYRSTGAIIRAANRLIAHNAGQRPKRLFTAAPEGRPVVAHEAETEVDEAKWVVQRIR
EECCCCHHHHHHHHHHHHHHCCCCCCCHHHEECCCCCCEEEECCCCCHHHHHHHHHHHHH
AGAEAGRRWGDFAILYRTTAQGRPFEEELREASIPYQVVGGPRFYDRREVQDSLAYLRLV
HHHHHCCCCCCEEEEEEECCCCCCHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHH
HNPRDSVSLERVLNAPRRGIGERGLQRLLALAAQRGVDLIAALRVAADEGVLQDEAAAGA
CCCCCHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
RQLHDHLRRAREALERQPGRFAAAMERLLRESGYLAYLQEEDRRKEERRSEVVESLIAAM
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHH
HEFERRSPDGGLGAYLDHLALLEGQEDESGGDVVKLQTIHSAKGLEYPVVFVVGMEQGLL
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCC
PYQRALEEGNLEEERRLCYVAITRAREELYLTGARVRWQRGEMVLTTPSQFLSEALGPGP
HHHHHHHCCCCHHHHHEEHHHHHHHHHHHEEECCEEEEECCCEEEECHHHHHHHHCCCCC
AQPLWVPASAD
CCCEEECCCCC
>Mature Secondary Structure 
SDLEAIVEGLNPAQRAAALHQDGPLLVIAGAGAGKTRTATHRLACLLARGVPPEAVMCI
CHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEE
TFTNKAAREMRDRAVALVGADARRVMIRTFHSAAMVLLRQYIGDFPESGRTPSFTVADPT
EECCHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCH
IQLALLKEAIAERNFDLKANRPESFLWRIGRYKNELADPESLLARQPSHDLMDWSRVLEM
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHH
IRSTDRYVNRLTAEIWKRYEEKLRRNDMVDFDDLINLFVQMLREKPDIRADLQRRFTYLQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHEEE
VDEFQDTNVAQLHMVKLLAGKRQNVMAVGDDAQSIYSWRSADIRCILEFERHFPGARTIL
EECCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCEEEEEHHHHCCCCEEEE
LEQNYRSTGAIIRAANRLIAHNAGQRPKRLFTAAPEGRPVVAHEAETEVDEAKWVVQRIR
EECCCCHHHHHHHHHHHHHHCCCCCCCHHHEECCCCCCEEEECCCCCHHHHHHHHHHHHH
AGAEAGRRWGDFAILYRTTAQGRPFEEELREASIPYQVVGGPRFYDRREVQDSLAYLRLV
HHHHHCCCCCCEEEEEEECCCCCCHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHH
HNPRDSVSLERVLNAPRRGIGERGLQRLLALAAQRGVDLIAALRVAADEGVLQDEAAAGA
CCCCCHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
RQLHDHLRRAREALERQPGRFAAAMERLLRESGYLAYLQEEDRRKEERRSEVVESLIAAM
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHH
HEFERRSPDGGLGAYLDHLALLEGQEDESGGDVVKLQTIHSAKGLEYPVVFVVGMEQGLL
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCC
PYQRALEEGNLEEERRLCYVAITRAREELYLTGARVRWQRGEMVLTTPSQFLSEALGPGP
HHHHHHHCCCCHHHHHEEHHHHHHHHHHHEEECCEEEEECCCEEEECHHHHHHHHCCCCC
AQPLWVPASAD
CCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]