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The map label for this gene is narB [H]

Identifier: 51893194

GI number: 51893194

Start: 2193251

End: 2195455

Strand: Reverse

Name: narB [H]

Synonym: STH2056

Alternate gene names: 51893194

Gene position: 2195455-2193251 (Counterclockwise)

Preceding gene: 51893195

Following gene: 51893193

Centisome position: 61.56

GC content: 68.62

Gene sequence:

>2205_bases
ATGACAGGCGGGATCAGCCGCCGCACGTTCCTGCAGCTCTCGGTGGCCGCGACGGCGGCCCTGGGGGCAGCCGGCGCCGT
CGCCGCAGGGGACGGCCGCAGTTACGTGAGCGGCGGGTTCGCACCCGGGCACGTGGAGCCGGACACGTGGACCTACTCCA
CCTGCGGCTTCTGCGGCACCGGGTGTGGGATCGAGATCGGCACGAAAGACGGGGTTCCCGTGGCCATCCGCGGGACCAAG
GGGTATCCGGTGAACGACGGCCTGCTCTGCGCCAAGGGCATCTATCAGTGGAAGATCATTACGGCGCCCGACCGGGGCAA
GCGCCCGCTCATCCGGAAGGACGGGAAGATGGTGCCGGCGACCTGGGACGAGGCGCTGACGGCCATGACCGACAAACTGA
AGCAGCTGCTGGCCGACTACGGGCCGGAGTCCATCGCCTGCTACAACACCGGCCAGCTCTGCATGGAGGAGTTCTACACC
CTCGGCAAGCTGATGCGGGCGGGTTTCCGCACCCCGAATCTGGACGGCAACACCCGGACCTGCATGGCCTCGGCCGTGAT
GGGTCATATCCGCTCCTTCGGCACCGACGGCCCCGTGGGCGCCTACGCGGACATCGAGGCGGCCGAGGTGATCCTGATCG
CCGGCTCCAACATGGCCGAGTGCCACCCGATCCTGTTCGGCCGGGTGGTGAAGGCGGTGGAGAAGGGGGCCAGGCTGATC
TGCGTCGACCCGCGGCTCACCCAGGTGAGCCGGATCGCGACCATCAACCTGCCCATCCGGCCGGGAACCGACGTGGTGCT
CCTGAACGCCATGGCCCACGTCATCATCCGGGAGGGGCTCATCGACCGGGCGTACATCGACGCGCACACCACCGGCTACC
CGGAACTGGAGCAGCACCTGCGGAAGTACACGCCCCAGTACGCCGCCGGGATCTGCGGCGTGCCGGCAGAGGACATCGAG
GCGGCCGCCCTGCTCTACGGACGGGCCGAGCGGTCGCTCTCCATGTGGGTGATGGGCATCAACCAGTCGGTCAACGGCAC
CATCGCCAACAACTGCTTCCACAACCTGAACCTGATCCAGGGCAAGATCGGCAAGCCGGGCTGCGCCTCCTTCTCCATCA
CCGGGCAGCCCGCGGCGATGGCCTCCCGGGAGGTGGGCGGCTCCTCTTCGTATCCCGGCTACCGGGCCATCGGCAACGCC
CAGCACCGGGCCGAGATCGCGGCGCTCTGGGGCGTGGATCCGGCAACGCTGCCGGACAAGTCCCCGTCCATCACCGAGAT
CATCGACGCCATCCTGGAGGGCAGGATCAAGGCGCTCTGGGTCATCTGCACCAACCCGCTCATGTCGCTGCCGGACCAGA
ACCGGGTGCGGCAGGCGCTGGAGAAGCTGGAGCTGCTGGTGGTGCAGGACGCTTTCGAGACCGTGGACACCGCCCAGTAC
GCCCACATCTTCCTGCCGGCGGCGCTCTGGGCCGAGAAGAGCGGCATCATGGTCAACTCGGAGCGGCGGCTCAACCTGCT
GAAGCCGGCGGTGAAGCCGCCCGGCGAGGCCCGGACGGACTTCGAGATCTTTCTGGACGTCGCGGATCGCATGGGCTTCG
GCCACCTGTTCCCCTACCGCACCACCGAGGAGTGCTTCGAGGAGATCAAGCGGGTGACCCGGGGACGGCCCAACGACTAC
TCCGGCGCGACCTACGCCCGCATCGAGGCCAGCAAGGGGCTCCAGTGGCCCATCCCGGATGAGCGGTCGCCCGGAACCCC
GCGGCTCTACGCCGACGGACGGTTCAACACCCCCGACGGGCGGGCCGTGCTGTGGGCCGTGGATCCCATCGAGCTTCCCG
AGGTCCCCGACGGCGAGTACCCCTTCAACCTGAACAGCGGCCGGGTGCAGGAGCACTACCACACCGGCACCAAGACCCGC
AAGGTCCCGGAGCTGAACGCCCTGGTGCCCACCGCTTACGTCGAGCTGAACCCCCGGGACGCGGAGGCCCTGGGCGTTCA
GTACGGCGACCGGGTGTGCATCGAGAGCCGGCGTGGCCAGATCGAGGCGAAGGTGGTGGTCACCTCCCAGGTGGCCCCGG
GCTCCTGCTTCGTCCCGATGCACTTTGGCGAGGGCCCCGTGAACCGGCTGACCGTCTGGGCGGTCGACCCGTTCTCCAAG
GAGCCCAACTACAAGCAGCAGGCCGTGCGCCTGCGCAGGGTGTGA

Upstream 100 bases:

>100_bases
ATGCGCCTTTCTTTGGCCAACAGGGTACTGGGATGGGAAGGGGCCCCTCCATACAATTTGAAGCAAGATCTTTTGTTTAA
GCGTTAAAGGAGGTTGTCCC

Downstream 100 bases:

>100_bases
GGGGGGACCGCGGATGACGACACAGCTCGGCTTTCACGTGGACATGGAGAAGTGCATCGGCTGCAAGGCATGCGAGGCGG
CCTGCAAGGACTTCTACCGG

Product: nitrate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 734; Mature: 733

Protein sequence:

>734_residues
MTGGISRRTFLQLSVAATAALGAAGAVAAGDGRSYVSGGFAPGHVEPDTWTYSTCGFCGTGCGIEIGTKDGVPVAIRGTK
GYPVNDGLLCAKGIYQWKIITAPDRGKRPLIRKDGKMVPATWDEALTAMTDKLKQLLADYGPESIACYNTGQLCMEEFYT
LGKLMRAGFRTPNLDGNTRTCMASAVMGHIRSFGTDGPVGAYADIEAAEVILIAGSNMAECHPILFGRVVKAVEKGARLI
CVDPRLTQVSRIATINLPIRPGTDVVLLNAMAHVIIREGLIDRAYIDAHTTGYPELEQHLRKYTPQYAAGICGVPAEDIE
AAALLYGRAERSLSMWVMGINQSVNGTIANNCFHNLNLIQGKIGKPGCASFSITGQPAAMASREVGGSSSYPGYRAIGNA
QHRAEIAALWGVDPATLPDKSPSITEIIDAILEGRIKALWVICTNPLMSLPDQNRVRQALEKLELLVVQDAFETVDTAQY
AHIFLPAALWAEKSGIMVNSERRLNLLKPAVKPPGEARTDFEIFLDVADRMGFGHLFPYRTTEECFEEIKRVTRGRPNDY
SGATYARIEASKGLQWPIPDERSPGTPRLYADGRFNTPDGRAVLWAVDPIELPEVPDGEYPFNLNSGRVQEHYHTGTKTR
KVPELNALVPTAYVELNPRDAEALGVQYGDRVCIESRRGQIEAKVVVTSQVAPGSCFVPMHFGEGPVNRLTVWAVDPFSK
EPNYKQQAVRLRRV

Sequences:

>Translated_734_residues
MTGGISRRTFLQLSVAATAALGAAGAVAAGDGRSYVSGGFAPGHVEPDTWTYSTCGFCGTGCGIEIGTKDGVPVAIRGTK
GYPVNDGLLCAKGIYQWKIITAPDRGKRPLIRKDGKMVPATWDEALTAMTDKLKQLLADYGPESIACYNTGQLCMEEFYT
LGKLMRAGFRTPNLDGNTRTCMASAVMGHIRSFGTDGPVGAYADIEAAEVILIAGSNMAECHPILFGRVVKAVEKGARLI
CVDPRLTQVSRIATINLPIRPGTDVVLLNAMAHVIIREGLIDRAYIDAHTTGYPELEQHLRKYTPQYAAGICGVPAEDIE
AAALLYGRAERSLSMWVMGINQSVNGTIANNCFHNLNLIQGKIGKPGCASFSITGQPAAMASREVGGSSSYPGYRAIGNA
QHRAEIAALWGVDPATLPDKSPSITEIIDAILEGRIKALWVICTNPLMSLPDQNRVRQALEKLELLVVQDAFETVDTAQY
AHIFLPAALWAEKSGIMVNSERRLNLLKPAVKPPGEARTDFEIFLDVADRMGFGHLFPYRTTEECFEEIKRVTRGRPNDY
SGATYARIEASKGLQWPIPDERSPGTPRLYADGRFNTPDGRAVLWAVDPIELPEVPDGEYPFNLNSGRVQEHYHTGTKTR
KVPELNALVPTAYVELNPRDAEALGVQYGDRVCIESRRGQIEAKVVVTSQVAPGSCFVPMHFGEGPVNRLTVWAVDPFSK
EPNYKQQAVRLRRV
>Mature_733_residues
TGGISRRTFLQLSVAATAALGAAGAVAAGDGRSYVSGGFAPGHVEPDTWTYSTCGFCGTGCGIEIGTKDGVPVAIRGTKG
YPVNDGLLCAKGIYQWKIITAPDRGKRPLIRKDGKMVPATWDEALTAMTDKLKQLLADYGPESIACYNTGQLCMEEFYTL
GKLMRAGFRTPNLDGNTRTCMASAVMGHIRSFGTDGPVGAYADIEAAEVILIAGSNMAECHPILFGRVVKAVEKGARLIC
VDPRLTQVSRIATINLPIRPGTDVVLLNAMAHVIIREGLIDRAYIDAHTTGYPELEQHLRKYTPQYAAGICGVPAEDIEA
AALLYGRAERSLSMWVMGINQSVNGTIANNCFHNLNLIQGKIGKPGCASFSITGQPAAMASREVGGSSSYPGYRAIGNAQ
HRAEIAALWGVDPATLPDKSPSITEIIDAILEGRIKALWVICTNPLMSLPDQNRVRQALEKLELLVVQDAFETVDTAQYA
HIFLPAALWAEKSGIMVNSERRLNLLKPAVKPPGEARTDFEIFLDVADRMGFGHLFPYRTTEECFEEIKRVTRGRPNDYS
GATYARIEASKGLQWPIPDERSPGTPRLYADGRFNTPDGRAVLWAVDPIELPEVPDGEYPFNLNSGRVQEHYHTGTKTRK
VPELNALVPTAYVELNPRDAEALGVQYGDRVCIESRRGQIEAKVVVTSQVAPGSCFVPMHFGEGPVNRLTVWAVDPFSKE
PNYKQQAVRLRRV

Specific function: Catalytic subunit of the nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC. Essential function for nitrate assimilation and may have a role in a

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Secreted (Potential). Note=Membrane- associated (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=838, Percent_Identity=32.4582338902148, Blast_Score=413, Evalue=1e-116,
Organism=Escherichia coli, GI3868721, Length=691, Percent_Identity=30.9696092619392, Blast_Score=354, Evalue=1e-98,
Organism=Escherichia coli, GI3868720, Length=667, Percent_Identity=24.4377811094453, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI87081797, Length=732, Percent_Identity=25.2732240437158, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI3868719, Length=666, Percent_Identity=23.5735735735736, Blast_Score=140, Evalue=4e-34,
Organism=Escherichia coli, GI171474008, Length=828, Percent_Identity=24.7584541062802, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI1787870, Length=773, Percent_Identity=24.0620957309185, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI145693196, Length=682, Percent_Identity=24.3401759530792, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI87081994, Length=687, Percent_Identity=23.5807860262009, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI1787778, Length=378, Percent_Identity=24.0740740740741, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311
- InterPro:   IPR019546 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; PF10518 TAT_signal [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 79822; Mature: 79691

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGGISRRTFLQLSVAATAALGAAGAVAAGDGRSYVSGGFAPGHVEPDTWTYSTCGFCGT
CCCCCCCCEEEEEHHHHHHHHCCCCCEECCCCCHHHCCCCCCCCCCCCCEEECCCCCCCC
GCGIEIGTKDGVPVAIRGTKGYPVNDGLLCAKGIYQWKIITAPDRGKRPLIRKDGKMVPA
CCCEEECCCCCCEEEEECCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCCCCCC
TWDEALTAMTDKLKQLLADYGPESIACYNTGQLCMEEFYTLGKLMRAGFRTPNLDGNTRT
CHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
CMASAVMGHIRSFGTDGPVGAYADIEAAEVILIAGSNMAECHPILFGRVVKAVEKGARLI
HHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEE
CVDPRLTQVSRIATINLPIRPGTDVVLLNAMAHVIIREGLIDRAYIDAHTTGYPELEQHL
EECCCHHCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHH
RKYTPQYAAGICGVPAEDIEAAALLYGRAERSLSMWVMGINQSVNGTIANNCFHNLNLIQ
HHCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEHHHHHHCCCEEE
GKIGKPGCASFSITGQPAAMASREVGGSSSYPGYRAIGNAQHRAEIAALWGVDPATLPDK
CCCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCCC
SPSITEIIDAILEGRIKALWVICTNPLMSLPDQNRVRQALEKLELLVVQDAFETVDTAQY
CCCHHHHHHHHHCCCEEEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHIFLPAALWAEKSGIMVNSERRLNLLKPAVKPPGEARTDFEIFLDVADRMGFGHLFPYR
HHHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCCCCCCCHHHHEEHHHHCCCCCCCCCC
TTEECFEEIKRVTRGRPNDYSGATYARIEASKGLQWPIPDERSPGTPRLYADGRFNTPDG
CHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
RAVLWAVDPIELPEVPDGEYPFNLNSGRVQEHYHTGTKTRKVPELNALVPTAYVELNPRD
CEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEECCCC
AEALGVQYGDRVCIESRRGQIEAKVVVTSQVAPGSCFVPMHFGEGPVNRLTVWAVDPFSK
CHHHCCCCCCEEEEECCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCC
EPNYKQQAVRLRRV
CCCHHHHHHHHHCC
>Mature Secondary Structure 
TGGISRRTFLQLSVAATAALGAAGAVAAGDGRSYVSGGFAPGHVEPDTWTYSTCGFCGT
CCCCCCCEEEEEHHHHHHHHCCCCCEECCCCCHHHCCCCCCCCCCCCCEEECCCCCCCC
GCGIEIGTKDGVPVAIRGTKGYPVNDGLLCAKGIYQWKIITAPDRGKRPLIRKDGKMVPA
CCCEEECCCCCCEEEEECCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCCCCCC
TWDEALTAMTDKLKQLLADYGPESIACYNTGQLCMEEFYTLGKLMRAGFRTPNLDGNTRT
CHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
CMASAVMGHIRSFGTDGPVGAYADIEAAEVILIAGSNMAECHPILFGRVVKAVEKGARLI
HHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEE
CVDPRLTQVSRIATINLPIRPGTDVVLLNAMAHVIIREGLIDRAYIDAHTTGYPELEQHL
EECCCHHCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHH
RKYTPQYAAGICGVPAEDIEAAALLYGRAERSLSMWVMGINQSVNGTIANNCFHNLNLIQ
HHCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEHHHHHHCCCEEE
GKIGKPGCASFSITGQPAAMASREVGGSSSYPGYRAIGNAQHRAEIAALWGVDPATLPDK
CCCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCCC
SPSITEIIDAILEGRIKALWVICTNPLMSLPDQNRVRQALEKLELLVVQDAFETVDTAQY
CCCHHHHHHHHHCCCEEEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHIFLPAALWAEKSGIMVNSERRLNLLKPAVKPPGEARTDFEIFLDVADRMGFGHLFPYR
HHHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCCCCCCCHHHHEEHHHHCCCCCCCCCC
TTEECFEEIKRVTRGRPNDYSGATYARIEASKGLQWPIPDERSPGTPRLYADGRFNTPDG
CHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
RAVLWAVDPIELPEVPDGEYPFNLNSGRVQEHYHTGTKTRKVPELNALVPTAYVELNPRD
CEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEECCCC
AEALGVQYGDRVCIESRRGQIEAKVVVTSQVAPGSCFVPMHFGEGPVNRLTVWAVDPFSK
CHHHCCCCCCEEEEECCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCC
EPNYKQQAVRLRRV
CCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA