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The map label for this gene is cbiB [H]

Identifier: 51893065

GI number: 51893065

Start: 2070064

End: 2071002

Strand: Reverse

Name: cbiB [H]

Synonym: STH1927

Alternate gene names: 51893065

Gene position: 2071002-2070064 (Counterclockwise)

Preceding gene: 51893066

Following gene: 51893064

Centisome position: 58.07

GC content: 75.4

Gene sequence:

>939_bases
ATGTTTCCCGCGGCGCCCTGGTACCTGCCGCTGGCGGCCCTCGCGGTGGACGCGGCGGTGGGCGATCCCGCCCGGCTGCC
CCATCCGGTGGCGCTCATGGGCCGGGTGATTGCGTGGGCCGAGCCGCGACTGCGGCGGACGCGGCTGCCGCTGCGGGCGG
CCGGGGTGCTGCTGGCCCTCCTGCTGCCGGTCGCGGCCTGGGGCCTCACCTGGGGGCTCATCCGGCTGGCCGCGGCCCTG
CATCCCTGGCTCGGTGTGGCCGCCGAGGTCTGGCTCCTCAGCACCTGCCTGGCGGCCCGCTCTCTGGCCGATCACGCACT
GGCGGTCTACCGCCCGCTGAAGGCCGGGGACCTCAGCGAGGCGCGGCGCCGGGTGGCGCACATCGTCGGCAGGGACACGG
CCGGGCTGGACGCCGCCGAGGTCACCCGGGCGACGGTGGAGACGGTGGCCGAGTCCACCTGCGACGGCGTGATCGCGCCG
CTCTTCTGGGGGCTGGTCGGCGGCGCGCCGCTGGCGATGGCCTACAAGGCCGCCAACACCCTGGATTCGATGGTCGGCCA
CCGGAACGAGCGCTACCTGGAGTTCGGCTGGGCCTCCGCCCGGCTGGATGATCTGGTCAACCTGGCGCCTGCCCGGCTCA
GCGCCCTGCTGCTGGCCCTGGCCGGCCTGAGCCCGAAGGCCCTCCGCATCGCCCTGCGGGACGCGCCGCGCCACCCCAGC
CCCAACAGCGGCTGGCCGGAGGCGGCGATGGCGGCCCTGCTGAGGGTGCAGCTGGGCGGCCTCAACCACTACGGCGGGAG
GCCGGAGCGGCGGGCCCACATGGGCGATCCGCTGGAGATGCTGCAGCCGGGTCACATCGTGCAGGCGGTGCGGTGGATGT
GGCTGGCGACCCTGCTCGGGACGGGCCTCGGGGCGCTGACGCTCTGGTTCTTGGCGTGA

Upstream 100 bases:

>100_bases
GGCTGGCGCGGGAGGCGGACCAGGTCTACGCCTGCGTGGCCGGTATCGCCGTCGACCTGAGGCGGATCGGTACGGTGATC
CCGGAGTGAGCGTGGCGGAA

Downstream 100 bases:

>100_bases
GGGGGTGACCGGGTGCGCATCGCGCTCTCCTTTCTGACCCGGATTCCCATGGGCCGACTTGAGACCCCGGACTACATGCG
GGATCTCGGCCGCGCGGCGG

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MFPAAPWYLPLAALAVDAAVGDPARLPHPVALMGRVIAWAEPRLRRTRLPLRAAGVLLALLLPVAAWGLTWGLIRLAAAL
HPWLGVAAEVWLLSTCLAARSLADHALAVYRPLKAGDLSEARRRVAHIVGRDTAGLDAAEVTRATVETVAESTCDGVIAP
LFWGLVGGAPLAMAYKAANTLDSMVGHRNERYLEFGWASARLDDLVNLAPARLSALLLALAGLSPKALRIALRDAPRHPS
PNSGWPEAAMAALLRVQLGGLNHYGGRPERRAHMGDPLEMLQPGHIVQAVRWMWLATLLGTGLGALTLWFLA

Sequences:

>Translated_312_residues
MFPAAPWYLPLAALAVDAAVGDPARLPHPVALMGRVIAWAEPRLRRTRLPLRAAGVLLALLLPVAAWGLTWGLIRLAAAL
HPWLGVAAEVWLLSTCLAARSLADHALAVYRPLKAGDLSEARRRVAHIVGRDTAGLDAAEVTRATVETVAESTCDGVIAP
LFWGLVGGAPLAMAYKAANTLDSMVGHRNERYLEFGWASARLDDLVNLAPARLSALLLALAGLSPKALRIALRDAPRHPS
PNSGWPEAAMAALLRVQLGGLNHYGGRPERRAHMGDPLEMLQPGHIVQAVRWMWLATLLGTGLGALTLWFLA
>Mature_312_residues
MFPAAPWYLPLAALAVDAAVGDPARLPHPVALMGRVIAWAEPRLRRTRLPLRAAGVLLALLLPVAAWGLTWGLIRLAAAL
HPWLGVAAEVWLLSTCLAARSLADHALAVYRPLKAGDLSEARRRVAHIVGRDTAGLDAAEVTRATVETVAESTCDGVIAP
LFWGLVGGAPLAMAYKAANTLDSMVGHRNERYLEFGWASARLDDLVNLAPARLSALLLALAGLSPKALRIALRDAPRHPS
PNSGWPEAAMAALLRVQLGGLNHYGGRPERRAHMGDPLEMLQPGHIVQAVRWMWLATLLGTGLGALTLWFLA

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group. However, the true cosubstrate could be (R)-1-amino-2-propanol O-2-phosphate, leading to cobinamide phosphate [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 33388; Mature: 33388

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPAAPWYLPLAALAVDAAVGDPARLPHPVALMGRVIAWAEPRLRRTRLPLRAAGVLLAL
CCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHH
LLPVAAWGLTWGLIRLAAALHPWLGVAAEVWLLSTCLAARSLADHALAVYRPLKAGDLSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
ARRRVAHIVGRDTAGLDAAEVTRATVETVAESTCDGVIAPLFWGLVGGAPLAMAYKAANT
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHH
LDSMVGHRNERYLEFGWASARLDDLVNLAPARLSALLLALAGLSPKALRIALRDAPRHPS
HHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
PNSGWPEAAMAALLRVQLGGLNHYGGRPERRAHMGDPLEMLQPGHIVQAVRWMWLATLLG
CCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHH
TGLGALTLWFLA
CCHHHHHHHHCC
>Mature Secondary Structure
MFPAAPWYLPLAALAVDAAVGDPARLPHPVALMGRVIAWAEPRLRRTRLPLRAAGVLLAL
CCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHH
LLPVAAWGLTWGLIRLAAALHPWLGVAAEVWLLSTCLAARSLADHALAVYRPLKAGDLSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
ARRRVAHIVGRDTAGLDAAEVTRATVETVAESTCDGVIAPLFWGLVGGAPLAMAYKAANT
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHH
LDSMVGHRNERYLEFGWASARLDDLVNLAPARLSALLLALAGLSPKALRIALRDAPRHPS
HHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
PNSGWPEAAMAALLRVQLGGLNHYGGRPERRAHMGDPLEMLQPGHIVQAVRWMWLATLLG
CCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHH
TGLGALTLWFLA
CCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA