Click here to switch to the map view.

The map label for this gene is cobS [H]

Identifier: 51893064

GI number: 51893064

Start: 2069239

End: 2070051

Strand: Reverse

Name: cobS [H]

Synonym: STH1926

Alternate gene names: 51893064

Gene position: 2070051-2069239 (Counterclockwise)

Preceding gene: 51893065

Following gene: 51893063

Centisome position: 58.05

GC content: 75.28

Gene sequence:

>813_bases
GTGCGCATCGCGCTCTCCTTTCTGACCCGGATTCCCATGGGCCGACTTGAGACCCCGGACTACATGCGGGATCTCGGCCG
CGCGGCGGGGTTCTTCCCCCTGGTGGGCCTGCTGGTGGGGGCCATCTGCCTGATCGCCTGGCTTGCGGGCAGGCTGCTCT
TCGGGCCTGCCGTGGCGGGCGTCGCGGCCGTGGCCGCGGGCCTCGGTGCGACCGGTGCGGTCCACCTGGACGGCCTGATG
GACGCCGCCGACGGGCTGCTCTCCGCCCGGAGCCGCGAGCGGATGCTGGAGATCATGAAAGATTCCCGGGTCGGCGTGAT
GGGCGCAGCGGCGGGAGCCCTGGCCCTGATGCTGCGGTGGGCCCTGCTGCTGGAGCTGACACCGCCGCGGGCAGCAGCCG
CCCTGCTGGTGGCCCCGGCCGTGGGGCGGATGGTGATCCCCCTGGCGGCGGTGCGCTGGCCGCCCGCCCGTGCCGAAGGG
ATGGGCGCTGCCTTCGGCCGGCACGTGGGCGGGGCGCAGCTGGCCGGGGCGCTTCTGGGCGGCCTCACGCTGGCTGTCGG
GGTGCCGGGGCTCCTGGCGTGGTGGGGGCAGAGCCCGGCAGCCCCGCCGGGCCTGACGGCAGCCGTGACGGTCTCCGCCC
TGCGGGGGCTGGGCGCGTGGCTCGCCGCCCTCGGGGTCGGTCTGGCCGGCGGGCGGTGGCTCTCGCGGCGACTCGGCGGC
CTGACCGGCGACACTTACGGAGCCTTGTGCGAGCTGGCGGAACTGGCGGCGATCGCCTGCTTCGCTCTGCAGACAGGGAG
GTGGACAGGATGA

Upstream 100 bases:

>100_bases
TCACATCGTGCAGGCGGTGCGGTGGATGTGGCTGGCGACCCTGCTCGGGACGGGCCTCGGGGCGCTGACGCTCTGGTTCT
TGGCGTGAGGGGGTGACCGG

Downstream 100 bases:

>100_bases
TGACCCTGCACGGCGGCGACCTGGCTGTGGCGCGGGCCCGCTGGGGCGAGCCGCCAGGGGGTTTCTGCGACTTCAGCGCC
AACATCAACCCGCTGGGCCC

Product: cobalamin-5-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MRIALSFLTRIPMGRLETPDYMRDLGRAAGFFPLVGLLVGAICLIAWLAGRLLFGPAVAGVAAVAAGLGATGAVHLDGLM
DAADGLLSARSRERMLEIMKDSRVGVMGAAAGALALMLRWALLLELTPPRAAAALLVAPAVGRMVIPLAAVRWPPARAEG
MGAAFGRHVGGAQLAGALLGGLTLAVGVPGLLAWWGQSPAAPPGLTAAVTVSALRGLGAWLAALGVGLAGGRWLSRRLGG
LTGDTYGALCELAELAAIACFALQTGRWTG

Sequences:

>Translated_270_residues
MRIALSFLTRIPMGRLETPDYMRDLGRAAGFFPLVGLLVGAICLIAWLAGRLLFGPAVAGVAAVAAGLGATGAVHLDGLM
DAADGLLSARSRERMLEIMKDSRVGVMGAAAGALALMLRWALLLELTPPRAAAALLVAPAVGRMVIPLAAVRWPPARAEG
MGAAFGRHVGGAQLAGALLGGLTLAVGVPGLLAWWGQSPAAPPGLTAAVTVSALRGLGAWLAALGVGLAGGRWLSRRLGG
LTGDTYGALCELAELAAIACFALQTGRWTG
>Mature_270_residues
MRIALSFLTRIPMGRLETPDYMRDLGRAAGFFPLVGLLVGAICLIAWLAGRLLFGPAVAGVAAVAAGLGATGAVHLDGLM
DAADGLLSARSRERMLEIMKDSRVGVMGAAAGALALMLRWALLLELTPPRAAAALLVAPAVGRMVIPLAAVRWPPARAEG
MGAAFGRHVGGAQLAGALLGGLTLAVGVPGLLAWWGQSPAAPPGLTAAVTVSALRGLGAWLAALGVGLAGGRWLSRRLGG
LTGDTYGALCELAELAAIACFALQTGRWTG

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=258, Percent_Identity=36.046511627907, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 27340; Mature: 27340

Theoretical pI: Translated: 11.33; Mature: 11.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALSFLTRIPMGRLETPDYMRDLGRAAGFFPLVGLLVGAICLIAWLAGRLLFGPAVAG
CCHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAAVAAGLGATGAVHLDGLMDAADGLLSARSRERMLEIMKDSRVGVMGAAAGALALMLRW
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ALLLELTPPRAAAALLVAPAVGRMVIPLAAVRWPPARAEGMGAAFGRHVGGAQLAGALLG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHH
GLTLAVGVPGLLAWWGQSPAAPPGLTAAVTVSALRGLGAWLAALGVGLAGGRWLSRRLGG
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
LTGDTYGALCELAELAAIACFALQTGRWTG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRIALSFLTRIPMGRLETPDYMRDLGRAAGFFPLVGLLVGAICLIAWLAGRLLFGPAVAG
CCHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAAVAAGLGATGAVHLDGLMDAADGLLSARSRERMLEIMKDSRVGVMGAAAGALALMLRW
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ALLLELTPPRAAAALLVAPAVGRMVIPLAAVRWPPARAEGMGAAFGRHVGGAQLAGALLG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHH
GLTLAVGVPGLLAWWGQSPAAPPGLTAAVTVSALRGLGAWLAALGVGLAGGRWLSRRLGG
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
LTGDTYGALCELAELAAIACFALQTGRWTG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11889109 [H]