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The map label for this gene is spoIIAC [H]

Identifier: 51892950

GI number: 51892950

Start: 1957001

End: 1957768

Strand: Reverse

Name: spoIIAC [H]

Synonym: STH1812

Alternate gene names: 51892950

Gene position: 1957768-1957001 (Counterclockwise)

Preceding gene: 51892951

Following gene: 51892949

Centisome position: 54.9

GC content: 71.35

Gene sequence:

>768_bases
ATGGCTGAGGAGAACACCCTGGAGGAGCGGGGCTGGCCCGATGAGGAGGTCCGGCGGCTGGTGGCCCTGGCCAGGGCGGG
GGACAAGGAGGCCCGGGACCGGCTGGTGGAGGGGAACCTCCGGCTGGTGCGATCGCTGGTCTCCCGCTTCAGCGCCTCCG
GGGCCGACCCCGAGGACCTGTTTCAGATCGGCTGCATCGGCCTTCTGAAGGCCATTGACCGGTTTGACCTGAACTACGAC
GTGCGGTTCAGCACCTACGCCGTGCCGCTGATCATGGGCGAGATCCGGCGCCACCTGCGGGACGACGGCCCGATGCGGGT
GAGCCGCAGCCTGAAGCAGCTGGCGCAGCAGGCCCGGCGGGTGCGGGAGCGGCTGGCCGCGCAGATGGAGCGGGACCCCA
CGGTCGCCGAGGTGGCGGCGGAGCTGGGCGTCTCGCCCGAGGAGGTGGTCGCGGCGCTGGACGGCGTGCGTCCGCCGACC
TCGATCCACCAGACCGTGCACGAGGGCGACGGCGACCCGATCTACCTGCTGGACCAGCTGGCGGCGGACGACGGACAGCG
GGAGGGCGTCTGGCTCGACCGCGTCGCCCTGCGGGAAGGGCTGAACCAGCTGGATGAGCGGGAGCGCACCGTCATCCTGC
TGCGGTTCTTCCGCGACAAGACCCAGACGGAGGTCGCGTCGATCCTGGGCTGCAGCCAGGTTCAGGTCTCCCGCCTGGAG
CGCAGGGCGCTGGAGCGGATCCGGAACTACGTGAACGCGGCCCCGTGA

Upstream 100 bases:

>100_bases
GTGGAGGTGACGTCGCAGGTCGGACAGGGCACCCGGGTGGTCATGCGGAAGCGGCCCGCGGCCAGGGACGTCGCCTCGCT
GAAGGGCAGCTGATCGGGCG

Downstream 100 bases:

>100_bases
ACAGCGCGGGCAGCGCGCAGACGGCCCGTGTAAGGAGCCGGGTTGCGGGGCAATGGTAGGAGCAGGGGTGGCAGGAAGAT
GCGCGCGGTCAACCCGTGGA

Product: sporulation sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MAEENTLEERGWPDEEVRRLVALARAGDKEARDRLVEGNLRLVRSLVSRFSASGADPEDLFQIGCIGLLKAIDRFDLNYD
VRFSTYAVPLIMGEIRRHLRDDGPMRVSRSLKQLAQQARRVRERLAAQMERDPTVAEVAAELGVSPEEVVAALDGVRPPT
SIHQTVHEGDGDPIYLLDQLAADDGQREGVWLDRVALREGLNQLDERERTVILLRFFRDKTQTEVASILGCSQVQVSRLE
RRALERIRNYVNAAP

Sequences:

>Translated_255_residues
MAEENTLEERGWPDEEVRRLVALARAGDKEARDRLVEGNLRLVRSLVSRFSASGADPEDLFQIGCIGLLKAIDRFDLNYD
VRFSTYAVPLIMGEIRRHLRDDGPMRVSRSLKQLAQQARRVRERLAAQMERDPTVAEVAAELGVSPEEVVAALDGVRPPT
SIHQTVHEGDGDPIYLLDQLAADDGQREGVWLDRVALREGLNQLDERERTVILLRFFRDKTQTEVASILGCSQVQVSRLE
RRALERIRNYVNAAP
>Mature_254_residues
AEENTLEERGWPDEEVRRLVALARAGDKEARDRLVEGNLRLVRSLVSRFSASGADPEDLFQIGCIGLLKAIDRFDLNYDV
RFSTYAVPLIMGEIRRHLRDDGPMRVSRSLKQLAQQARRVRERLAAQMERDPTVAEVAAELGVSPEEVVAALDGVRPPTS
IHQTVHEGDGDPIYLLDQLAADDGQREGVWLDRVALREGLNQLDERERTVILLRFFRDKTQTEVASILGCSQVQVSRLER
RALERIRNYVNAAP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=257, Percent_Identity=28.0155642023346, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789871, Length=262, Percent_Identity=29.3893129770992, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788231, Length=206, Percent_Identity=31.0679611650485, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789098, Length=235, Percent_Identity=27.6595744680851, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28836; Mature: 28705

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEENTLEERGWPDEEVRRLVALARAGDKEARDRLVEGNLRLVRSLVSRFSASGADPEDL
CCCCCCCHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHH
FQIGCIGLLKAIDRFDLNYDVRFSTYAVPLIMGEIRRHLRDDGPMRVSRSLKQLAQQARR
HHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VRERLAAQMERDPTVAEVAAELGVSPEEVVAALDGVRPPTSIHQTVHEGDGDPIYLLDQL
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHH
AADDGQREGVWLDRVALREGLNQLDERERTVILLRFFRDKTQTEVASILGCSQVQVSRLE
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRALERIRNYVNAAP
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEENTLEERGWPDEEVRRLVALARAGDKEARDRLVEGNLRLVRSLVSRFSASGADPEDL
CCCCCCHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHH
FQIGCIGLLKAIDRFDLNYDVRFSTYAVPLIMGEIRRHLRDDGPMRVSRSLKQLAQQARR
HHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VRERLAAQMERDPTVAEVAAELGVSPEEVVAALDGVRPPTSIHQTVHEGDGDPIYLLDQL
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHH
AADDGQREGVWLDRVALREGLNQLDERERTVILLRFFRDKTQTEVASILGCSQVQVSRLE
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRALERIRNYVNAAP
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2513372 [H]