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The map label for this gene is miaA

Identifier: 51892885

GI number: 51892885

Start: 1892459

End: 1893418

Strand: Reverse

Name: miaA

Synonym: STH1747

Alternate gene names: 51892885

Gene position: 1893418-1892459 (Counterclockwise)

Preceding gene: 51892886

Following gene: 51892884

Centisome position: 53.09

GC content: 69.38

Gene sequence:

>960_bases
GTGAAGCTGCCACTCGTGGTGCTGGTGGGCCCCACCGCAGTGGGCAAGACCGCCCTGTCCGTGGCGGTGGCCCAGGCGGT
GGGGGCGGAGATCATATCCGGCGACTCGATGCAGGTCTACCGGGGCATGGACATCGGCACCGCCAAGATCCGCCCGGAGG
AGATGGGCGGGGTGCCGCACCACCTCATCGACATCAAGGATCCCGACGAGGAGTTCTCGGTGGCCGAGTTCCAGGCCCGG
GTGGACGCGCTGATCCCGCAGATCTGCGCCCGGGGCCGGCTGCCCATGCTGGTGGGCGGCACCGGCCTTTACGTGCGCGC
AGTCGTGGAGAAGTATACCTTCACGCCGATGGAGGCCGACCACGAGTTACGGGCGCGGCTCAGGCAGGAGGAGGAGCGGC
ACGGGCCCGGGTACCTGCACGCCCGGCTCCGGGAGGTGGATCCGGCCTCCGCCGCACGGCTCCACCCCAACGACCTCCTC
CGCATCGTGCGGGCGCTGGAGGTCTACGAGCAGACCGGCGTGCCCATTTCCGCCACGCAGACCGCGGCTTTCTCAGAGCC
CCGGTACGACGACCTGATGATCGGGCTCACCATGGATCGGGCGCAGCTCTACGCCCGCATCGACGAGCGGGTGGACGCCA
TGCTGGCCGCCGGCTGGCTGGACGAGGTGCGGGGCTTGCTGGTCCGGTACCCGCCCCACGTGCGGGCGATGCAGGCCCTG
GGGTACCGGGAGCTGGTGCTCTACCTGCGCGGGCTGCTGACGTGGGAAGAGGCGGTGGCACTGATCAAGCGGAACACCCG
CCGCTTCGCCAAGCGGCAGTTTACCTGGTTCCGGAAGGAGCGGCGGCTCACCTGGCTGGATCTCACCGGGCCGGAGGCCC
GCAACCGGGCGACGGAGGAGATCGTCAGACTGATCAGGGAGAAATGGCCCTGTAGAGAGAGGAAGGGGCGGATCGAGTGA

Upstream 100 bases:

>100_bases
TCAGCGACCTGGCGGCCTGCGCGTCGCCCGAGACCTGTCCCCACGGGCGGCCGACCGTCATCTGCGTGTCCATATCCGAG
CTGGAGAAGAGGTTCAAGCG

Downstream 100 bases:

>100_bases
CCAAGGCGTCAGCCAGCCTGCAGGACGGGTTCCTCAACCTGCTGCGCAGGGAGAACATCCCGGCCACCATCTATCTGGTG
AACGGATACCAGCTGAAAGG

Product: tRNA isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKLPLVVLVGPTAVGKTALSVAVAQAVGAEIISGDSMQVYRGMDIGTAKIRPEEMGGVPHHLIDIKDPDEEFSVAEFQAR
VDALIPQICARGRLPMLVGGTGLYVRAVVEKYTFTPMEADHELRARLRQEEERHGPGYLHARLREVDPASAARLHPNDLL
RIVRALEVYEQTGVPISATQTAAFSEPRYDDLMIGLTMDRAQLYARIDERVDAMLAAGWLDEVRGLLVRYPPHVRAMQAL
GYRELVLYLRGLLTWEEAVALIKRNTRRFAKRQFTWFRKERRLTWLDLTGPEARNRATEEIVRLIREKWPCRERKGRIE

Sequences:

>Translated_319_residues
MKLPLVVLVGPTAVGKTALSVAVAQAVGAEIISGDSMQVYRGMDIGTAKIRPEEMGGVPHHLIDIKDPDEEFSVAEFQAR
VDALIPQICARGRLPMLVGGTGLYVRAVVEKYTFTPMEADHELRARLRQEEERHGPGYLHARLREVDPASAARLHPNDLL
RIVRALEVYEQTGVPISATQTAAFSEPRYDDLMIGLTMDRAQLYARIDERVDAMLAAGWLDEVRGLLVRYPPHVRAMQAL
GYRELVLYLRGLLTWEEAVALIKRNTRRFAKRQFTWFRKERRLTWLDLTGPEARNRATEEIVRLIREKWPCRERKGRIE
>Mature_319_residues
MKLPLVVLVGPTAVGKTALSVAVAQAVGAEIISGDSMQVYRGMDIGTAKIRPEEMGGVPHHLIDIKDPDEEFSVAEFQAR
VDALIPQICARGRLPMLVGGTGLYVRAVVEKYTFTPMEADHELRARLRQEEERHGPGYLHARLREVDPASAARLHPNDLL
RIVRALEVYEQTGVPISATQTAAFSEPRYDDLMIGLTMDRAQLYARIDERVDAMLAAGWLDEVRGLLVRYPPHVRAMQAL
GYRELVLYLRGLLTWEEAVALIKRNTRRFAKRQFTWFRKERRLTWLDLTGPEARNRATEEIVRLIREKWPCRERKGRIE

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=313, Percent_Identity=35.1437699680511, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1790613, Length=303, Percent_Identity=40.9240924092409, Blast_Score=211, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI25144712, Length=301, Percent_Identity=30.5647840531561, Blast_Score=131, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=32.5342465753425, Blast_Score=159, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24649830, Length=268, Percent_Identity=35.0746268656716, Blast_Score=150, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_SYMTH (Q67NL1)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_075576.1
- ProteinModelPortal:   Q67NL1
- SMR:   Q67NL1
- GeneID:   2979338
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH1747
- NMPDR:   fig|292459.1.peg.1685
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- BioCyc:   STHE292459:STH1747-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36286; Mature: 36286

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPLVVLVGPTAVGKTALSVAVAQAVGAEIISGDSMQVYRGMDIGTAKIRPEEMGGVPH
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCC
HLIDIKDPDEEFSVAEFQARVDALIPQICARGRLPMLVGGTGLYVRAVVEKYTFTPMEAD
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCC
HELRARLRQEEERHGPGYLHARLREVDPASAARLHPNDLLRIVRALEVYEQTGVPISATQ
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCC
TAAFSEPRYDDLMIGLTMDRAQLYARIDERVDAMLAAGWLDEVRGLLVRYPPHVRAMQAL
HHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHC
GYRELVLYLRGLLTWEEAVALIKRNTRRFAKRQFTWFRKERRLTWLDLTGPEARNRATEE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHH
IVRLIREKWPCRERKGRIE
HHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLPLVVLVGPTAVGKTALSVAVAQAVGAEIISGDSMQVYRGMDIGTAKIRPEEMGGVPH
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCC
HLIDIKDPDEEFSVAEFQARVDALIPQICARGRLPMLVGGTGLYVRAVVEKYTFTPMEAD
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCC
HELRARLRQEEERHGPGYLHARLREVDPASAARLHPNDLLRIVRALEVYEQTGVPISATQ
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCC
TAAFSEPRYDDLMIGLTMDRAQLYARIDERVDAMLAAGWLDEVRGLLVRYPPHVRAMQAL
HHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHC
GYRELVLYLRGLLTWEEAVALIKRNTRRFAKRQFTWFRKERRLTWLDLTGPEARNRATEE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHH
IVRLIREKWPCRERKGRIE
HHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA