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The map label for this gene is dat [H]

Identifier: 51892468

GI number: 51892468

Start: 1473742

End: 1474587

Strand: Reverse

Name: dat [H]

Synonym: STH1330

Alternate gene names: 51892468

Gene position: 1474587-1473742 (Counterclockwise)

Preceding gene: 51892469

Following gene: 51892463

Centisome position: 41.35

GC content: 70.57

Gene sequence:

>846_bases
GTGTACCTGAACGGTCAGTACGTCCCGTATGAAGAGGCGAAGATCCCGGTGGAGGACCGGGCCTTCCTGTTCGCCGACGG
CATCTACGAGGTGGTGCGGGTCTACGGCGGCCGGCCGTTCGACCTGGAGCCGCACCTCCGGCGGCTCGCCCGCAGCGCCC
GGGAGCTCCGTCTGCCTGAGCCCGACCTGGAGGGGCTGACCCGGGTGACCCACGAGCTCATCCGCCGCAACGGCGTGGAT
GAGGGGACCGTCTACTACCAGATCAGCCGGGGCGTGCACGCCCCCCGTGCCCACGCCTTTCCTGGCCAGCCGGTGGAACC
GACGGTGCTCGTGCTCACCCACCCGCTGACGCCCGAGGCGGTGGCGGCGGGCGAGCGGCGTCGGCAGGAAGGGGTCACGG
CCATCACGGTGCCTGAGCAGCGGTGGGCCCGGTGCGACATCAAGAGCGTTTCGCTCCTGCCCAACGTCCTGGCCAAGCAG
CAGGCCGCCGAGCAGGGGGCCTACGAGGCGCTCTTCGTCCGGGACGGCTTCGTCATCGAGGGCAGCAGCAGCAACGTGTT
CGCGGTCATCGACGGGGCCATCGTCACCTATCCGGCCTGCAACTATATCCTGAACGGCATCGCCCGCCAGCGGGTCATCC
GGGACGCCCGGTCGCTGGGGTACACCGTGCTGGAGCAGGGGATTCCGCTGGCGGCCCTGGATCGCTGCCAGGAACTGTTC
GTGACCTCGACCACATCCGAAGTGACGCCGGTGGTCGCCGTGGACGGCAGGCCCATCGGCGACGGCAAGGTGGGGCCGGT
GGTGCGGGCCCTGCAGCAGGCCTACCGCGCCGCGGCGCGGGGGTAA

Upstream 100 bases:

>100_bases
ATCCGCCCGGCCGGTCCGGCAGGAGACCGGTGAGCGGATCCCGTAATGTCCTCTGAAATCATTCTACCACCACGGGGGAG
GCGGGCGCATGTCGTCAACC

Downstream 100 bases:

>100_bases
GTATCCGACGGGAGAGAACCGGCGGGCCTGGGCCTCCAGGCCCGCCGGAGCGCGCTCACCGCCCCGCCGGCCCGGCGAGG
AACCGGACGCAGGGCGCGAT

Product: D-alanine aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MYLNGQYVPYEEAKIPVEDRAFLFADGIYEVVRVYGGRPFDLEPHLRRLARSARELRLPEPDLEGLTRVTHELIRRNGVD
EGTVYYQISRGVHAPRAHAFPGQPVEPTVLVLTHPLTPEAVAAGERRRQEGVTAITVPEQRWARCDIKSVSLLPNVLAKQ
QAAEQGAYEALFVRDGFVIEGSSSNVFAVIDGAIVTYPACNYILNGIARQRVIRDARSLGYTVLEQGIPLAALDRCQELF
VTSTTSEVTPVVAVDGRPIGDGKVGPVVRALQQAYRAAARG

Sequences:

>Translated_281_residues
MYLNGQYVPYEEAKIPVEDRAFLFADGIYEVVRVYGGRPFDLEPHLRRLARSARELRLPEPDLEGLTRVTHELIRRNGVD
EGTVYYQISRGVHAPRAHAFPGQPVEPTVLVLTHPLTPEAVAAGERRRQEGVTAITVPEQRWARCDIKSVSLLPNVLAKQ
QAAEQGAYEALFVRDGFVIEGSSSNVFAVIDGAIVTYPACNYILNGIARQRVIRDARSLGYTVLEQGIPLAALDRCQELF
VTSTTSEVTPVVAVDGRPIGDGKVGPVVRALQQAYRAAARG
>Mature_281_residues
MYLNGQYVPYEEAKIPVEDRAFLFADGIYEVVRVYGGRPFDLEPHLRRLARSARELRLPEPDLEGLTRVTHELIRRNGVD
EGTVYYQISRGVHAPRAHAFPGQPVEPTVLVLTHPLTPEAVAAGERRRQEGVTAITVPEQRWARCDIKSVSLLPNVLAKQ
QAAEQGAYEALFVRDGFVIEGSSSNVFAVIDGAIVTYPACNYILNGIARQRVIRDARSLGYTVLEQGIPLAALDRCQELF
VTSTTSEVTPVVAVDGRPIGDGKVGPVVRALQQAYRAAARG

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=291, Percent_Identity=27.1477663230241, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 30915; Mature: 30915

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLNGQYVPYEEAKIPVEDRAFLFADGIYEVVRVYGGRPFDLEPHLRRLARSARELRLPE
CCCCCCCCCHHCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
PDLEGLTRVTHELIRRNGVDEGTVYYQISRGVHAPRAHAFPGQPVEPTVLVLTHPLTPEA
CCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHH
VAAGERRRQEGVTAITVPEQRWARCDIKSVSLLPNVLAKQQAAEQGAYEALFVRDGFVIE
HHHHHHHHHCCCEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEE
GSSSNVFAVIDGAIVTYPACNYILNGIARQRVIRDARSLGYTVLEQGIPLAALDRCQELF
CCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VTSTTSEVTPVVAVDGRPIGDGKVGPVVRALQQAYRAAARG
HCCCCCCCCEEEEECCEECCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYLNGQYVPYEEAKIPVEDRAFLFADGIYEVVRVYGGRPFDLEPHLRRLARSARELRLPE
CCCCCCCCCHHCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
PDLEGLTRVTHELIRRNGVDEGTVYYQISRGVHAPRAHAFPGQPVEPTVLVLTHPLTPEA
CCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHH
VAAGERRRQEGVTAITVPEQRWARCDIKSVSLLPNVLAKQQAAEQGAYEALFVRDGFVIE
HHHHHHHHHCCCEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEE
GSSSNVFAVIDGAIVTYPACNYILNGIARQRVIRDARSLGYTVLEQGIPLAALDRCQELF
CCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VTSTTSEVTPVVAVDGRPIGDGKVGPVVRALQQAYRAAARG
HCCCCCCCCEEEEECCEECCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2644261; 2914916; 7626635; 9538014; 9749913 [H]