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The map label for this gene is appA [H]

Identifier: 51892176

GI number: 51892176

Start: 1155392

End: 1157245

Strand: Reverse

Name: appA [H]

Synonym: STH1038

Alternate gene names: 51892176

Gene position: 1157245-1155392 (Counterclockwise)

Preceding gene: 51892181

Following gene: 51892175

Centisome position: 32.45

GC content: 64.72

Gene sequence:

>1854_bases
ATGGGCGCGGATGTGCAGTGGGATGAGTCCACCCGCACGGTGACGGCCACCCGGGGCAACACCACGGTGCAGCTGGTCAT
CGGCAGCACCACCGCCCGGGTGAACGGGCAGGCCCGGACCCTGGACGCCGCCCCGTTCATCCAGAACGGCCGCACCCTGG
TTCCGCTGCGGTTCGTGAGCGAGGCGTTCGGCGCCGAGGTGGAGTATGACCCCGCCACCACGGCGATCTCCATCGTCGAC
CCGAACTGGCCGCGCCGGGGCGGGCGGCTGAACCTGGCCATGTGGAACAAGCCGATCGGGGCCTTCAACCCGATCATCGT
CAATGACACATACGGCTCCAACCTTACCGGCCTGATGTACGAAGGCCTGTGGAAGTATGACGAGCGCTATATCCCCGTGC
CCGCCCTCGCCGAGGCATGGGAGTGGGACGAGACCAACACGAAGCTGACCTTCTACCTGCGCCGGAACGCGACGTTCTTC
GATGGCACCCCGGTGACCGCCAAGGATGTCGTCTTCACGTACAAGGCCATTTGGCACCCCAGGTACATCGGCCCGCGTGA
CTCCGGTTGGGAGGACGTCGTCGGGTACGAAGATTACGCCAACGGCATCAGCGGCGAAACCCGTGAGAACTTCGAGCGGG
GCATCGTCACCACCGGCAACCTGCCCGGTCTCTACGCGGTAGACGACTACACCGTGGTCTTCCAGCTGAAGCAGCCGAAC
GCGCCGTTCCTCTACAACATCGCCTACGGGATCGTGGACAGCAGCAAGTACGGCAGCATCCCGGTGCAGGACTGGGGTAC
GGCCAATGACCCGTACAACATCTTCCCCAACGGCAACGGCCCCTTCAAGATGAAGGAGTATGTTGACGGCCAGTACGCCA
TCCTGGAGGCCAACCCGAACTACTGGGCAGGCCGGCCTTACATCGACGAGGTCGTCTGGCGCATCGTGGCCGCTGAGGTG
GCCGTCGGTGAGATGCAGCGCGGCACGCTGGACTACGTGGAGTTCAGCCCCAACGAGCTGGACGCCTACGCGGCGATGCC
ACATGTGAAGATCTACGAGTTCCCGGACATGGTCTTCCAGGAGATGGTCTACAACACCGGTCCCAACGCCCGGGGTGAGA
TGGGACCCACCACCGACGTGCGGGTGCGCCGCGCCATCAACCATGCGATCAACCGGCAGGCCATCATCAACAACCTGATG
GGCGGCCACGCCTCCATGATGTACGGTCCGATCCACCCGCTCACCTGGGCGTACACGGAGGACGTCGAGCAGTATCCGTA
TGATCCCGACAAGGCTCGTCAGCTGCTCGAAGAGGCCGGCTGGGTCCTGGGCAGCGACGGGTACCGGTACAAGGACGGGA
AGCGGCTGACCCTGCGGCTGCTGTACCCCAACGTCGGCAACCCAGTCCGGCAGGCGACGGCGCCCGTGGTGCAGCAGTGG
CTGAAGGAGGTCGGCATTGAGGTCATCCTGGAGGGTTACGACTGGCCGACGCTGGATGAGAAGCGGGCGGCCCGGGACTT
CGACATGTACTTCATCGGCTTCTCGCTGGGCAACTCCGACCCGGACCCGACGGGCCTGTGGGACAAGGCGGCCACCGAGC
CCGGCGGCTTCAACGACAGCCAGTGGTGGACGGAGAAGTCGGAGCAGCTGATCGCCGCCGGCAAGGCCACCAACGACATC
GAGGAGCGCATCGAGATCTACCACGAGTGGGCGCGCCACTGGACGGAAGAGACCCCGGCGCTCATCCTGTACGCAGTGAA
CACCTTGATCGCAGCCAACGACCGTCTGCAGAACTTCAAGCCCGGCCCGCAGGGCTACCTCTGGAACATCGAGGAGCTGT
GGCTCTCCCGGTGA

Upstream 100 bases:

>100_bases
GTACCCGGTCACGTTCAACGGCCACCCGGTGATGTTCGACGTGGAGCCGGTGAACGTGAACAACCGCGTCTTCGTCCCGT
TCCGGGCGATCTTCGAGAAG

Downstream 100 bases:

>100_bases
TCCTCTGACCATCCACCGGCTGGCGGCCCGGGGGGAACGCACCCCCGGGCCGCGCACCGCCGGGAAGAAAGGAGACTGAC
AGGTGGGCCGGTACCTCGTG

Product: oligopeptide ABC transporter substrate-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MGADVQWDESTRTVTATRGNTTVQLVIGSTTARVNGQARTLDAAPFIQNGRTLVPLRFVSEAFGAEVEYDPATTAISIVD
PNWPRRGGRLNLAMWNKPIGAFNPIIVNDTYGSNLTGLMYEGLWKYDERYIPVPALAEAWEWDETNTKLTFYLRRNATFF
DGTPVTAKDVVFTYKAIWHPRYIGPRDSGWEDVVGYEDYANGISGETRENFERGIVTTGNLPGLYAVDDYTVVFQLKQPN
APFLYNIAYGIVDSSKYGSIPVQDWGTANDPYNIFPNGNGPFKMKEYVDGQYAILEANPNYWAGRPYIDEVVWRIVAAEV
AVGEMQRGTLDYVEFSPNELDAYAAMPHVKIYEFPDMVFQEMVYNTGPNARGEMGPTTDVRVRRAINHAINRQAIINNLM
GGHASMMYGPIHPLTWAYTEDVEQYPYDPDKARQLLEEAGWVLGSDGYRYKDGKRLTLRLLYPNVGNPVRQATAPVVQQW
LKEVGIEVILEGYDWPTLDEKRAARDFDMYFIGFSLGNSDPDPTGLWDKAATEPGGFNDSQWWTEKSEQLIAAGKATNDI
EERIEIYHEWARHWTEETPALILYAVNTLIAANDRLQNFKPGPQGYLWNIEELWLSR

Sequences:

>Translated_617_residues
MGADVQWDESTRTVTATRGNTTVQLVIGSTTARVNGQARTLDAAPFIQNGRTLVPLRFVSEAFGAEVEYDPATTAISIVD
PNWPRRGGRLNLAMWNKPIGAFNPIIVNDTYGSNLTGLMYEGLWKYDERYIPVPALAEAWEWDETNTKLTFYLRRNATFF
DGTPVTAKDVVFTYKAIWHPRYIGPRDSGWEDVVGYEDYANGISGETRENFERGIVTTGNLPGLYAVDDYTVVFQLKQPN
APFLYNIAYGIVDSSKYGSIPVQDWGTANDPYNIFPNGNGPFKMKEYVDGQYAILEANPNYWAGRPYIDEVVWRIVAAEV
AVGEMQRGTLDYVEFSPNELDAYAAMPHVKIYEFPDMVFQEMVYNTGPNARGEMGPTTDVRVRRAINHAINRQAIINNLM
GGHASMMYGPIHPLTWAYTEDVEQYPYDPDKARQLLEEAGWVLGSDGYRYKDGKRLTLRLLYPNVGNPVRQATAPVVQQW
LKEVGIEVILEGYDWPTLDEKRAARDFDMYFIGFSLGNSDPDPTGLWDKAATEPGGFNDSQWWTEKSEQLIAAGKATNDI
EERIEIYHEWARHWTEETPALILYAVNTLIAANDRLQNFKPGPQGYLWNIEELWLSR
>Mature_616_residues
GADVQWDESTRTVTATRGNTTVQLVIGSTTARVNGQARTLDAAPFIQNGRTLVPLRFVSEAFGAEVEYDPATTAISIVDP
NWPRRGGRLNLAMWNKPIGAFNPIIVNDTYGSNLTGLMYEGLWKYDERYIPVPALAEAWEWDETNTKLTFYLRRNATFFD
GTPVTAKDVVFTYKAIWHPRYIGPRDSGWEDVVGYEDYANGISGETRENFERGIVTTGNLPGLYAVDDYTVVFQLKQPNA
PFLYNIAYGIVDSSKYGSIPVQDWGTANDPYNIFPNGNGPFKMKEYVDGQYAILEANPNYWAGRPYIDEVVWRIVAAEVA
VGEMQRGTLDYVEFSPNELDAYAAMPHVKIYEFPDMVFQEMVYNTGPNARGEMGPTTDVRVRRAINHAINRQAIINNLMG
GHASMMYGPIHPLTWAYTEDVEQYPYDPDKARQLLEEAGWVLGSDGYRYKDGKRLTLRLLYPNVGNPVRQATAPVVQQWL
KEVGIEVILEGYDWPTLDEKRAARDFDMYFIGFSLGNSDPDPTGLWDKAATEPGGFNDSQWWTEKSEQLIAAGKATNDIE
ERIEIYHEWARHWTEETPALILYAVNTLIAANDRLQNFKPGPQGYLWNIEELWLSR

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=466, Percent_Identity=28.1115879828326, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1787762, Length=483, Percent_Identity=25.0517598343685, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1787551, Length=542, Percent_Identity=25.2767527675277, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI87081878, Length=515, Percent_Identity=25.8252427184466, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1787495, Length=497, Percent_Identity=25.5533199195171, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787052, Length=508, Percent_Identity=24.0157480314961, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789887, Length=537, Percent_Identity=23.6499068901304, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1789397, Length=499, Percent_Identity=22.4448897795591, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 69896; Mature: 69765

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGADVQWDESTRTVTATRGNTTVQLVIGSTTARVNGQARTLDAAPFIQNGRTLVPLRFVS
CCCCCCCCCCCEEEEEECCCEEEEEEEECCEEEECCCEEEEECCCHHHCCCEEEEHHHHH
EAFGAEVEYDPATTAISIVDPNWPRRGGRLNLAMWNKPIGAFNPIIVNDTYGSNLTGLMY
HHHCCCEEECCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCHHHHH
EGLWKYDERYIPVPALAEAWEWDETNTKLTFYLRRNATFFDGTPVTAKDVVFTYKAIWHP
HHHHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEECCCEEECCCCCCHHHHEEEEHHHCCC
RYIGPRDSGWEDVVGYEDYANGISGETRENFERGIVTTGNLPGLYAVDDYTVVFQLKQPN
CCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHCCCEEECCCCCEEEECCEEEEEEEECCC
APFLYNIAYGIVDSSKYGSIPVQDWGTANDPYNIFPNGNGPFKMKEYVDGQYAILEANPN
CCEEEEEEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCEEEHHHCCCEEEEEECCCC
YWAGRPYIDEVVWRIVAAEVAVGEMQRGTLDYVEFSPNELDAYAAMPHVKIYEFPDMVFQ
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCEEEECCHHHHHH
EMVYNTGPNARGEMGPTTDVRVRRAINHAINRQAIINNLMGGHASMMYGPIHPLTWAYTE
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEHHHH
DVEQYPYDPDKARQLLEEAGWVLGSDGYRYKDGKRLTLRLLYPNVGNPVRQATAPVVQQW
HHHHCCCCHHHHHHHHHHCCCEECCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHH
LKEVGIEVILEGYDWPTLDEKRAARDFDMYFIGFSLGNSDPDPTGLWDKAATEPGGFNDS
HHHCCEEEEEECCCCCCCCHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
QWWTEKSEQLIAAGKATNDIEERIEIYHEWARHWTEETPALILYAVNTLIAANDRLQNFK
HHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCHHHCCC
PGPQGYLWNIEELWLSR
CCCCCCEECHHHHHCCC
>Mature Secondary Structure 
GADVQWDESTRTVTATRGNTTVQLVIGSTTARVNGQARTLDAAPFIQNGRTLVPLRFVS
CCCCCCCCCCEEEEEECCCEEEEEEEECCEEEECCCEEEEECCCHHHCCCEEEEHHHHH
EAFGAEVEYDPATTAISIVDPNWPRRGGRLNLAMWNKPIGAFNPIIVNDTYGSNLTGLMY
HHHCCCEEECCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCHHHHH
EGLWKYDERYIPVPALAEAWEWDETNTKLTFYLRRNATFFDGTPVTAKDVVFTYKAIWHP
HHHHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEECCCEEECCCCCCHHHHEEEEHHHCCC
RYIGPRDSGWEDVVGYEDYANGISGETRENFERGIVTTGNLPGLYAVDDYTVVFQLKQPN
CCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHCCCEEECCCCCEEEECCEEEEEEEECCC
APFLYNIAYGIVDSSKYGSIPVQDWGTANDPYNIFPNGNGPFKMKEYVDGQYAILEANPN
CCEEEEEEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCEEEHHHCCCEEEEEECCCC
YWAGRPYIDEVVWRIVAAEVAVGEMQRGTLDYVEFSPNELDAYAAMPHVKIYEFPDMVFQ
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCEEEECCHHHHHH
EMVYNTGPNARGEMGPTTDVRVRRAINHAINRQAIINNLMGGHASMMYGPIHPLTWAYTE
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEHHHH
DVEQYPYDPDKARQLLEEAGWVLGSDGYRYKDGKRLTLRLLYPNVGNPVRQATAPVVQQW
HHHHCCCCHHHHHHHHHHCCCEECCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHH
LKEVGIEVILEGYDWPTLDEKRAARDFDMYFIGFSLGNSDPDPTGLWDKAATEPGGFNDS
HHHCCEEEEEECCCCCCCCHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
QWWTEKSEQLIAAGKATNDIEERIEIYHEWARHWTEETPALILYAVNTLIAANDRLQNFK
HHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCHHHCCC
PGPQGYLWNIEELWLSR
CCCCCCEECHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]