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The map label for this gene is nrfA [H]

Identifier: 51891867

GI number: 51891867

Start: 813172

End: 814464

Strand: Reverse

Name: nrfA [H]

Synonym: STH729

Alternate gene names: 51891867

Gene position: 814464-813172 (Counterclockwise)

Preceding gene: 51891868

Following gene: 51891866

Centisome position: 22.84

GC content: 64.11

Gene sequence:

>1293_bases
ATGAACCGCAAGTGGATCATTGCCATCGGGGCCGCCCTGGCGGTCGTCCTGGTGGGAGTGGGGACCTTCACCTACGTCGC
CAACAAGACCGAGGCCGTCGAGGGGCCCGCCGTCAGCATTTCCCCGAACGAGACCGATCCTGCGGTCTTCAAGCTCCTGT
ACCCGGCCCACTACGACTCCTACATGCGCAACGGCGAGATGCACGAGCCGGCCTTGAAGTACGCTTCCTCCGAGAAGAAG
AAGAGCCGCCTCGACCAGTTCCCCTACATGCGCACCCTGTGGGCGGGCATGGCCTTCTCGAAGGAGTACAACGAGGCCCG
CGGCCACCTCTACACGCTGGATGACGTCGTCGGCGGCAACGGCACCGAGGCCACCCAGCGCATCAACGACAAGACCAACC
TGACCTGCATGTACTGCAAGTCGGCCCAGGTGCCCCAGCTGATCGAGCAGATGGGCGACGCTTTCTACAACACCAAGCTC
CTGGCCGACAACAACACCGACCTCTTCGTCCATCCCATCTCCTGTTCGGATTGCCATGATCCCCAGACGATGGAGCTGCG
CATCACCCGCCCGGCCCTGGTCGAGGCCATGGAGCGGATCGGCAGGCCCGTGGAGAACGCGACCCGCCAGGAGATGCGCT
CCCTCGTCTGCGCCCAGTGCCACGTGGAGTACTTCTTCAACCCCAACGACGCCAACAGGGTCTACTTCCCGTGGGACAAG
GGCTTCGAGCCTGAGGACATGTACGCCTACTACGAGGAGATCGGCTTCTCCGACTGGACCCATGCGCAGACGGGCGGCGG
CATGTTGAAGGCTCAGCATCCCGAGTTTGAAATGTTCCAGGGCAGCGTCCATCAGCGGGCCGGCCTCTCCTGCGCCGACT
GCCATATGCCGTACGTGACGGAGGGCTCCACCAAGATCTCCTCCCACTGGTGGACCTCGCCTTTCCGGACGTTCGAGCAG
TCCTGCGCCCAGTGCCACCGGGAGAGCCAGGAGGAGATGGCCCAGCGGGTTCTGCATACGCAGGACCGTGTGAAGGAGGC
CCTGGACCGGGCCGGCATCGCCAACCAGGATGCCATCCTGGCCATCGAAAAGGCCATCGCGGCCGGCGTGGATGAGGAGA
TCCTCAACCAGGCCCGGGCCCTGCACCGCGAGGCCCAGTTCTACTGGGATCTGGCGTCGGCCGAGAACTCCTTCGGTTTC
CACAACCCGCAGAAGTTCCTCGAGACCCTCGCCGATTCCATCGACCTGGCCCGCCAGGCCGAGCTCCTGGTCACCCGGGC
GATGAACAAGTAA

Upstream 100 bases:

>100_bases
GACGGACGGACTCAAGTGTTTCGAATGCCACCGTGACACTACGCACGGTCTTCCGCTCCGCAATTGATGTACTCGATCAT
GATGGGAGGCTGCACGAACC

Downstream 100 bases:

>100_bases
CCCTGCCCTGCCTCGATAGCCCCGGCCGCGCCGACCTGGCGCGCCCGGGGCTCCCGCGTCTGAGGGGGTACCGGGCGGCG
TCATATATGGCGTCATATAT

Product: cytochrome C nitrite reductase catalytic subunit

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNRKWIIAIGAALAVVLVGVGTFTYVANKTEAVEGPAVSISPNETDPAVFKLLYPAHYDSYMRNGEMHEPALKYASSEKK
KSRLDQFPYMRTLWAGMAFSKEYNEARGHLYTLDDVVGGNGTEATQRINDKTNLTCMYCKSAQVPQLIEQMGDAFYNTKL
LADNNTDLFVHPISCSDCHDPQTMELRITRPALVEAMERIGRPVENATRQEMRSLVCAQCHVEYFFNPNDANRVYFPWDK
GFEPEDMYAYYEEIGFSDWTHAQTGGGMLKAQHPEFEMFQGSVHQRAGLSCADCHMPYVTEGSTKISSHWWTSPFRTFEQ
SCAQCHRESQEEMAQRVLHTQDRVKEALDRAGIANQDAILAIEKAIAAGVDEEILNQARALHREAQFYWDLASAENSFGF
HNPQKFLETLADSIDLARQAELLVTRAMNK

Sequences:

>Translated_430_residues
MNRKWIIAIGAALAVVLVGVGTFTYVANKTEAVEGPAVSISPNETDPAVFKLLYPAHYDSYMRNGEMHEPALKYASSEKK
KSRLDQFPYMRTLWAGMAFSKEYNEARGHLYTLDDVVGGNGTEATQRINDKTNLTCMYCKSAQVPQLIEQMGDAFYNTKL
LADNNTDLFVHPISCSDCHDPQTMELRITRPALVEAMERIGRPVENATRQEMRSLVCAQCHVEYFFNPNDANRVYFPWDK
GFEPEDMYAYYEEIGFSDWTHAQTGGGMLKAQHPEFEMFQGSVHQRAGLSCADCHMPYVTEGSTKISSHWWTSPFRTFEQ
SCAQCHRESQEEMAQRVLHTQDRVKEALDRAGIANQDAILAIEKAIAAGVDEEILNQARALHREAQFYWDLASAENSFGF
HNPQKFLETLADSIDLARQAELLVTRAMNK
>Mature_430_residues
MNRKWIIAIGAALAVVLVGVGTFTYVANKTEAVEGPAVSISPNETDPAVFKLLYPAHYDSYMRNGEMHEPALKYASSEKK
KSRLDQFPYMRTLWAGMAFSKEYNEARGHLYTLDDVVGGNGTEATQRINDKTNLTCMYCKSAQVPQLIEQMGDAFYNTKL
LADNNTDLFVHPISCSDCHDPQTMELRITRPALVEAMERIGRPVENATRQEMRSLVCAQCHVEYFFNPNDANRVYFPWDK
GFEPEDMYAYYEEIGFSDWTHAQTGGGMLKAQHPEFEMFQGSVHQRAGLSCADCHMPYVTEGSTKISSHWWTSPFRTFEQ
SCAQCHRESQEEMAQRVLHTQDRVKEALDRAGIANQDAILAIEKAIAAGVDEEILNQARALHREAQFYWDLASAENSFGF
HNPQKFLETLADSIDLARQAELLVTRAMNK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=430, Percent_Identity=32.7906976744186, Blast_Score=224, Evalue=7e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 48675; Mature: 48675

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKWIIAIGAALAVVLVGVGTFTYVANKTEAVEGPAVSISPNETDPAVFKLLYPAHYDS
CCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
YMRNGEMHEPALKYASSEKKKSRLDQFPYMRTLWAGMAFSKEYNEARGHLYTLDDVVGGN
HHHCCCCCCHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCC
GTEATQRINDKTNLTCMYCKSAQVPQLIEQMGDAFYNTKLLADNNTDLFVHPISCSDCHD
CCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCCCC
PQTMELRITRPALVEAMERIGRPVENATRQEMRSLVCAQCHVEYFFNPNDANRVYFPWDK
CCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCC
GFEPEDMYAYYEEIGFSDWTHAQTGGGMLKAQHPEFEMFQGSVHQRAGLSCADCHMPYVT
CCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCHHHHHCCHHHHCCCCHHHCCCCEEE
EGSTKISSHWWTSPFRTFEQSCAQCHRESQEEMAQRVLHTQDRVKEALDRAGIANQDAIL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
AIEKAIAAGVDEEILNQARALHREAQFYWDLASAENSFGFHNPQKFLETLADSIDLARQA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELLVTRAMNK
HHHHHHHCCC
>Mature Secondary Structure
MNRKWIIAIGAALAVVLVGVGTFTYVANKTEAVEGPAVSISPNETDPAVFKLLYPAHYDS
CCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHH
YMRNGEMHEPALKYASSEKKKSRLDQFPYMRTLWAGMAFSKEYNEARGHLYTLDDVVGGN
HHHCCCCCCHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCC
GTEATQRINDKTNLTCMYCKSAQVPQLIEQMGDAFYNTKLLADNNTDLFVHPISCSDCHD
CCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCCCC
PQTMELRITRPALVEAMERIGRPVENATRQEMRSLVCAQCHVEYFFNPNDANRVYFPWDK
CCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCC
GFEPEDMYAYYEEIGFSDWTHAQTGGGMLKAQHPEFEMFQGSVHQRAGLSCADCHMPYVT
CCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCHHHHHCCHHHHCCCCHHHCCCCEEE
EGSTKISSHWWTSPFRTFEQSCAQCHRESQEEMAQRVLHTQDRVKEALDRAGIANQDAIL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
AIEKAIAAGVDEEILNQARALHREAQFYWDLASAENSFGFHNPQKFLETLADSIDLARQA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ELLVTRAMNK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]