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The map label for this gene is spoT [H]

Identifier: 51598458

GI number: 51598458

Start: 195286

End: 197289

Strand: Direct

Name: spoT [H]

Synonym: BG0196

Alternate gene names: 51598458

Gene position: 195286-197289 (Clockwise)

Preceding gene: 51598457

Following gene: 51598459

Centisome position: 21.6

GC content: 28.34

Gene sequence:

>2004_bases
ATGATACAAGCATATGAAATTGCACACTTAATAAAAATAAATGATCTTGAAAAAGCTAAAAATATTTTCAAAAAAACTGT
TGAGAATACTTATAAAGATGAATTTGAACGGAAAAGCATATTCAAAGCTCTAGAAATAGCAGAACAGCTTCACTATGGCC
AATACAGAGAAAGTGGAGAGCCTTACATTATTCATCCAATAATGGTTTCATTATTCCTTGCTAAATTTCAACTAGATTTT
AAAGCAACTATTGCCGGACTACTCCATGACGTACTTGAAGATACAAATATTGAAAAAGAAGAAATAGTAAAAGAATTCGA
CGAAGAAATTTTAAGTTTAATAGACGGTGTAACTAAAATTCATGATTTGCATAATAAAACAAGAAGCATAAAAGAAGCTA
ATACTATTTCAAAAATGTTTTTTGCAATGACACATGACATTAGAATAATAATAATAAAACTTGCAGACAAACTTCATAAT
ATGACAACTCTCTCTTATTTGCCTAAAAATAGACAAGATAGAATTGCAAAAGATTGCCTTTCAACTTATGTTCCAATAGC
AGAACGCCTTGGAATCTCATCTCTTAAAACATATCTTGAAGATCTTTCATTTAAACATCTTTATCCTAAAGATTATAAAG
AGATAAAAAATTTTTTATCTGAAACAAAGATAGAAAGAGAAAAAAAATTATACAAAGGTAAATTATCAATAGAAAAAGAA
CTTCAAAAAAGCGGAATTGAAGCAGAAATCACAGTAAGGTCAAAACATTTTTATTCAATATTTAGAAAAATGCAAACAAG
AACAAACAAGCTTACTCAAATTTTTGATACTTTGGGAATAAGAATAATTTGCAAAAAACAAAAAGAATGTTATGAAATAT
TAGAAATTGTTCACAGAGTGTGGAAACCAATCCCAGGAAGACTTAAAGATTATATTGCAAGTCCAAAAGAAAATAAATAT
CAATCACTACACACTACCGTAAGAATACCTGAAGATAATCAGCTTATTGAAATACAAATTCGAACAGAAGAAATGGACAG
AATTGCTAATTATGGGGTTGCAGCTCACTGGATATACAAAGAACAAATTGCACTTAAGGCTGATGATCTTTCATTTATAA
ACCGAATAAAAAAATGGCAACAAGAATCGGCCAACAAAAGTCAATATTCAATGAATGATATACATAAAGAGCTTTTAAAT
ACATTCATATATGTTTACACCCCAGAAGGAGAAGTTGTAGAACTTCCTTTTGGATCAAATTCAATTGATTTTGCATACAT
AATACACACAGATATTGGAGACCAAGCTCTTTATGCAAAAATAAATGGAAAAATAAGCTCAATCACAAAACCACTTAAAA
ACGAACAAATTGTTGAAATATTTACATCAAAAGATTCAAAACCAGATGTAATATGGCTAAACAGCGTAAGAACAAAAAAA
GCTCGATCAAAAATTAGATCATGGCTTAATAAAAATGACAATACAATTTTTGTAGACAACAATATTATTGCGTATCTTGT
TGGAGCAAACAAAGAACAAAGAAAGCTTTTTAGCTTATTCAAAGCTTATACTAAAACCAAAATAAAAAGAATTGCAATAG
ACTCTGAATGCAATCCTACAACAGGTGAAGACATTGTTGGAATAATACATAAAGATGAGATAATAGTGCATAATGAAAAT
TGTCAAAAGCTCAAGTACTATAAAAAAAATCAATTAATTGAAGTTGAGTGGGAAGCAACACCAACTAGAAAAGTGCATCA
CATTATTTTACTTTTAAAAGAATTGAAAGGAATATTTAGTTATCTTGAAAACATTTTCACAATCAATGACGTAAGACTTA
TTAGCGAAAAAATAGAAGACTGCGGGAATGGTCATGGAATAACAAACATAATAGTGTCATCAAATGCTAAAAATATAGCA
AAAATTATTTCCTCTTTAAAAGAAAACCCAAATATCTTACAAATAATGCAAGTAGAAGAAGATATTAAAAATTATGATAA
TTAA

Upstream 100 bases:

>100_bases
AAAGTTTAAAAGATTTTGCAATCAAAAAAGGATTTTCACATTTAAAAACCATTTATGATCTTGAAAAAAGAGCAAGAGCG
CTGGTATTAGGACAAATAAC

Downstream 100 bases:

>100_bases
GATATTATTAGCATTATTATTCATTAACTTGACAAGCATCCCTCTTGTACCAAAGAAAATGAAAAAAAATGTATCCAATT
TAAACAATAAAATTGCAAAC

Product: guanosine-3,5-bis(diphosphate) 3-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 667; Mature: 667

Protein sequence:

>667_residues
MIQAYEIAHLIKINDLEKAKNIFKKTVENTYKDEFERKSIFKALEIAEQLHYGQYRESGEPYIIHPIMVSLFLAKFQLDF
KATIAGLLHDVLEDTNIEKEEIVKEFDEEILSLIDGVTKIHDLHNKTRSIKEANTISKMFFAMTHDIRIIIIKLADKLHN
MTTLSYLPKNRQDRIAKDCLSTYVPIAERLGISSLKTYLEDLSFKHLYPKDYKEIKNFLSETKIEREKKLYKGKLSIEKE
LQKSGIEAEITVRSKHFYSIFRKMQTRTNKLTQIFDTLGIRIICKKQKECYEILEIVHRVWKPIPGRLKDYIASPKENKY
QSLHTTVRIPEDNQLIEIQIRTEEMDRIANYGVAAHWIYKEQIALKADDLSFINRIKKWQQESANKSQYSMNDIHKELLN
TFIYVYTPEGEVVELPFGSNSIDFAYIIHTDIGDQALYAKINGKISSITKPLKNEQIVEIFTSKDSKPDVIWLNSVRTKK
ARSKIRSWLNKNDNTIFVDNNIIAYLVGANKEQRKLFSLFKAYTKTKIKRIAIDSECNPTTGEDIVGIIHKDEIIVHNEN
CQKLKYYKKNQLIEVEWEATPTRKVHHIILLLKELKGIFSYLENIFTINDVRLISEKIEDCGNGHGITNIIVSSNAKNIA
KIISSLKENPNILQIMQVEEDIKNYDN

Sequences:

>Translated_667_residues
MIQAYEIAHLIKINDLEKAKNIFKKTVENTYKDEFERKSIFKALEIAEQLHYGQYRESGEPYIIHPIMVSLFLAKFQLDF
KATIAGLLHDVLEDTNIEKEEIVKEFDEEILSLIDGVTKIHDLHNKTRSIKEANTISKMFFAMTHDIRIIIIKLADKLHN
MTTLSYLPKNRQDRIAKDCLSTYVPIAERLGISSLKTYLEDLSFKHLYPKDYKEIKNFLSETKIEREKKLYKGKLSIEKE
LQKSGIEAEITVRSKHFYSIFRKMQTRTNKLTQIFDTLGIRIICKKQKECYEILEIVHRVWKPIPGRLKDYIASPKENKY
QSLHTTVRIPEDNQLIEIQIRTEEMDRIANYGVAAHWIYKEQIALKADDLSFINRIKKWQQESANKSQYSMNDIHKELLN
TFIYVYTPEGEVVELPFGSNSIDFAYIIHTDIGDQALYAKINGKISSITKPLKNEQIVEIFTSKDSKPDVIWLNSVRTKK
ARSKIRSWLNKNDNTIFVDNNIIAYLVGANKEQRKLFSLFKAYTKTKIKRIAIDSECNPTTGEDIVGIIHKDEIIVHNEN
CQKLKYYKKNQLIEVEWEATPTRKVHHIILLLKELKGIFSYLENIFTINDVRLISEKIEDCGNGHGITNIIVSSNAKNIA
KIISSLKENPNILQIMQVEEDIKNYDN
>Mature_667_residues
MIQAYEIAHLIKINDLEKAKNIFKKTVENTYKDEFERKSIFKALEIAEQLHYGQYRESGEPYIIHPIMVSLFLAKFQLDF
KATIAGLLHDVLEDTNIEKEEIVKEFDEEILSLIDGVTKIHDLHNKTRSIKEANTISKMFFAMTHDIRIIIIKLADKLHN
MTTLSYLPKNRQDRIAKDCLSTYVPIAERLGISSLKTYLEDLSFKHLYPKDYKEIKNFLSETKIEREKKLYKGKLSIEKE
LQKSGIEAEITVRSKHFYSIFRKMQTRTNKLTQIFDTLGIRIICKKQKECYEILEIVHRVWKPIPGRLKDYIASPKENKY
QSLHTTVRIPEDNQLIEIQIRTEEMDRIANYGVAAHWIYKEQIALKADDLSFINRIKKWQQESANKSQYSMNDIHKELLN
TFIYVYTPEGEVVELPFGSNSIDFAYIIHTDIGDQALYAKINGKISSITKPLKNEQIVEIFTSKDSKPDVIWLNSVRTKK
ARSKIRSWLNKNDNTIFVDNNIIAYLVGANKEQRKLFSLFKAYTKTKIKRIAIDSECNPTTGEDIVGIIHKDEIIVHNEN
CQKLKYYKKNQLIEVEWEATPTRKVHHIILLLKELKGIFSYLENIFTINDVRLISEKIEDCGNGHGITNIIVSSNAKNIA
KIISSLKENPNILQIMQVEEDIKNYDN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=611, Percent_Identity=34.5335515548282, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1789147, Length=450, Percent_Identity=35.3333333333333, Blast_Score=301, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 77744; Mature: 77744

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQAYEIAHLIKINDLEKAKNIFKKTVENTYKDEFERKSIFKALEIAEQLHYGQYRESGE
CCCHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
PYIIHPIMVSLFLAKFQLDFKATIAGLLHDVLEDTNIEKEEIVKEFDEEILSLIDGVTKI
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
HDLHNKTRSIKEANTISKMFFAMTHDIRIIIIKLADKLHNMTTLSYLPKNRQDRIAKDCL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCHHHHHHCCCCHHHHHHHHHH
STYVPIAERLGISSLKTYLEDLSFKHLYPKDYKEIKNFLSETKIEREKKLYKGKLSIEKE
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LQKSGIEAEITVRSKHFYSIFRKMQTRTNKLTQIFDTLGIRIICKKQKECYEILEIVHRV
HHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHH
WKPIPGRLKDYIASPKENKYQSLHTTVRIPEDNQLIEIQIRTEEMDRIANYGVAAHWIYK
HCCCCHHHHHHHCCCCCCHHHHHEEEEECCCCCCEEEEEEEHHHHHHHHHCCHHEEEEEH
EQIALKADDLSFINRIKKWQQESANKSQYSMNDIHKELLNTFIYVYTPEGEVVELPFGSN
HHHEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEECCCCCEEEECCCCC
SIDFAYIIHTDIGDQALYAKINGKISSITKPLKNEQIVEIFTSKDSKPDVIWLNSVRTKK
CCEEEEEEEECCCCCEEEEEECCCHHHHHCCCCCCCEEEEEECCCCCCCEEEECCHHHHH
ARSKIRSWLNKNDNTIFVDNNIIAYLVGANKEQRKLFSLFKAYTKTKIKRIAIDSECNPT
HHHHHHHHHCCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
TGEDIVGIIHKDEIIVHNENCQKLKYYKKNQLIEVEWEATPTRKVHHIILLLKELKGIFS
CCCHHEEEEECCEEEEECCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
YLENIFTINDVRLISEKIEDCGNGHGITNIIVSSNAKNIAKIISSLKENPNILQIMQVEE
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHH
DIKNYDN
HHHCCCC
>Mature Secondary Structure
MIQAYEIAHLIKINDLEKAKNIFKKTVENTYKDEFERKSIFKALEIAEQLHYGQYRESGE
CCCHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
PYIIHPIMVSLFLAKFQLDFKATIAGLLHDVLEDTNIEKEEIVKEFDEEILSLIDGVTKI
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
HDLHNKTRSIKEANTISKMFFAMTHDIRIIIIKLADKLHNMTTLSYLPKNRQDRIAKDCL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCHHHHHHCCCCHHHHHHHHHH
STYVPIAERLGISSLKTYLEDLSFKHLYPKDYKEIKNFLSETKIEREKKLYKGKLSIEKE
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LQKSGIEAEITVRSKHFYSIFRKMQTRTNKLTQIFDTLGIRIICKKQKECYEILEIVHRV
HHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHH
WKPIPGRLKDYIASPKENKYQSLHTTVRIPEDNQLIEIQIRTEEMDRIANYGVAAHWIYK
HCCCCHHHHHHHCCCCCCHHHHHEEEEECCCCCCEEEEEEEHHHHHHHHHCCHHEEEEEH
EQIALKADDLSFINRIKKWQQESANKSQYSMNDIHKELLNTFIYVYTPEGEVVELPFGSN
HHHEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEEEEECCCCCEEEECCCCC
SIDFAYIIHTDIGDQALYAKINGKISSITKPLKNEQIVEIFTSKDSKPDVIWLNSVRTKK
CCEEEEEEEECCCCCEEEEEECCCHHHHHCCCCCCCEEEEEECCCCCCCEEEECCHHHHH
ARSKIRSWLNKNDNTIFVDNNIIAYLVGANKEQRKLFSLFKAYTKTKIKRIAIDSECNPT
HHHHHHHHHCCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
TGEDIVGIIHKDEIIVHNENCQKLKYYKKNQLIEVEWEATPTRKVHHIILLLKELKGIFS
CCCHHEEEEECCEEEEECCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
YLENIFTINDVRLISEKIEDCGNGHGITNIIVSSNAKNIAKIISSLKENPNILQIMQVEE
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHH
DIKNYDN
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]