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The map label for this gene is hemK [H]

Identifier: 51598457

GI number: 51598457

Start: 194468

End: 195289

Strand: Direct

Name: hemK [H]

Synonym: BG0195

Alternate gene names: 51598457

Gene position: 194468-195289 (Clockwise)

Preceding gene: 51598456

Following gene: 51598458

Centisome position: 21.51

GC content: 26.28

Gene sequence:

>822_bases
ATGAATGTAGCCGAAATTATAAATTATGCTAAAAGTAAAAATTTAGATACAATAGAAGCACTACTAATACTTGAATTGAT
TTTAAAAACCAGAAAAGAATTAATAATTGCAAATATAAAAAAAAGTTTAACAAAAAGAGAAAAAAAACTTTTTTTTGATC
AAATAGATAAAATGAAAAAAGGAACTCCTATTCACTACATACTACAAAAAAAAGAATTTATGGGTATTGAGTTCAGCTTA
AACAAGCATGTCTTAATCCCAAGATTTGACACAGAATGTTTGGTAGAAGAAGCCTTAATTCAAATTCAACAAAATGGATT
TAAAAAAATATTAGACTTATGCTGCGGCAGCGGATGCATAGGGCTTTCTATTGCCTATTACACTAAAAAAAAAGTAACAC
TCTCAGATATTTCAATAAAAGCTTTAAAAATAGTCGAAAAAAATACAAAAAAGCTTAAACTTGAAAAATTTATAGAAATA
ATCCACTCTAATTTGCTAAAATTCATAAAGGAAAGGTTTGATATAATTATTACAAATCCTCCTTATTTAAACAAAGAAGA
ATTAGAAATAAAAAATAAAATAAAAAAAGAACCTGCAAAAGCTCTTTTAGGATTCGGAAAAGATGGGCTTAATATTTCTA
GAAAAATATTAAGCCAAGCAAAAGAAAAGCTTAACCCAAATGGACTCATAATAATAGAATCAGCTCCTTGGCAAATAAAA
AGTTTAAAAGATTTTGCAATCAAAAAAGGATTTTCACATTTAAAAACCATTTATGATCTTGAAAAAAGAGCAAGAGCGCT
GGTATTAGGACAAATAACATGA

Upstream 100 bases:

>100_bases
ATAAATTAGAAGAATTTATGCAAGGAGAGCTTGATCCACTTCTTGATCCTCTAACAATAGAGCTTCAAGAACAAAAATTT
AAAAGCAACAACATATAGTA

Downstream 100 bases:

>100_bases
TACAAGCATATGAAATTGCACACTTAATAAAAATAAATGATCTTGAAAAAGCTAAAAATATTTTCAAAAAAACTGTTGAG
AATACTTATAAAGATGAATT

Product: HemK family methylase

Products: NA

Alternate protein names: HemK protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNVAEIINYAKSKNLDTIEALLILELILKTRKELIIANIKKSLTKREKKLFFDQIDKMKKGTPIHYILQKKEFMGIEFSL
NKHVLIPRFDTECLVEEALIQIQQNGFKKILDLCCGSGCIGLSIAYYTKKKVTLSDISIKALKIVEKNTKKLKLEKFIEI
IHSNLLKFIKERFDIIITNPPYLNKEELEIKNKIKKEPAKALLGFGKDGLNISRKILSQAKEKLNPNGLIIIESAPWQIK
SLKDFAIKKGFSHLKTIYDLEKRARALVLGQIT

Sequences:

>Translated_273_residues
MNVAEIINYAKSKNLDTIEALLILELILKTRKELIIANIKKSLTKREKKLFFDQIDKMKKGTPIHYILQKKEFMGIEFSL
NKHVLIPRFDTECLVEEALIQIQQNGFKKILDLCCGSGCIGLSIAYYTKKKVTLSDISIKALKIVEKNTKKLKLEKFIEI
IHSNLLKFIKERFDIIITNPPYLNKEELEIKNKIKKEPAKALLGFGKDGLNISRKILSQAKEKLNPNGLIIIESAPWQIK
SLKDFAIKKGFSHLKTIYDLEKRARALVLGQIT
>Mature_273_residues
MNVAEIINYAKSKNLDTIEALLILELILKTRKELIIANIKKSLTKREKKLFFDQIDKMKKGTPIHYILQKKEFMGIEFSL
NKHVLIPRFDTECLVEEALIQIQQNGFKKILDLCCGSGCIGLSIAYYTKKKVTLSDISIKALKIVEKNTKKLKLEKFIEI
IHSNLLKFIKERFDIIITNPPYLNKEELEIKNKIKKEPAKALLGFGKDGLNISRKILSQAKEKLNPNGLIIIESAPWQIK
SLKDFAIKKGFSHLKTIYDLEKRARALVLGQIT

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=226, Percent_Identity=29.646017699115, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI87082085, Length=195, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787463, Length=270, Percent_Identity=25.1851851851852, Blast_Score=98, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324266, Length=160, Percent_Identity=31.25, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582226, Length=217, Percent_Identity=26.2672811059908, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 31336; Mature: 31336

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVAEIINYAKSKNLDTIEALLILELILKTRKELIIANIKKSLTKREKKLFFDQIDKMKK
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTPIHYILQKKEFMGIEFSLNKHVLIPRFDTECLVEEALIQIQQNGFKKILDLCCGSGCI
CCCCEEEEEHHHHCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
GLSIAYYTKKKVTLSDISIKALKIVEKNTKKLKLEKFIEIIHSNLLKFIKERFDIIITNP
HHEEEEEECCEEEECCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCC
PYLNKEELEIKNKIKKEPAKALLGFGKDGLNISRKILSQAKEKLNPNGLIIIESAPWQIK
CCCCHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHH
SLKDFAIKKGFSHLKTIYDLEKRARALVLGQIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MNVAEIINYAKSKNLDTIEALLILELILKTRKELIIANIKKSLTKREKKLFFDQIDKMKK
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTPIHYILQKKEFMGIEFSLNKHVLIPRFDTECLVEEALIQIQQNGFKKILDLCCGSGCI
CCCCEEEEEHHHHCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
GLSIAYYTKKKVTLSDISIKALKIVEKNTKKLKLEKFIEIIHSNLLKFIKERFDIIITNP
HHEEEEEECCEEEECCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCC
PYLNKEELEIKNKIKKEPAKALLGFGKDGLNISRKILSQAKEKLNPNGLIIIESAPWQIK
CCCCHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHH
SLKDFAIKKGFSHLKTIYDLEKRARALVLGQIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA