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The map label for this gene is nifS [H]

Identifier: 51598344

GI number: 51598344

Start: 79339

End: 80607

Strand: Direct

Name: nifS [H]

Synonym: BG0082

Alternate gene names: 51598344

Gene position: 79339-80607 (Clockwise)

Preceding gene: 51598341

Following gene: 51598346

Centisome position: 8.77

GC content: 26.08

Gene sequence:

>1269_bases
ATGGATTTCAAACAAATAAAAAGCAATGCAGAAAAGGTTAAGTTTTTAAGGAAAGATTTTCCTATTTTAAATAAACAGTT
TGACAATAAGCATATAATTTATTTTGACAATGCAGCAACCTCTCAAAAGCCTAAAAAAGTAATTTATTCTATCATTGAAT
ATTATGAAAATTACAACGCAAATGTACATAGAAGCGGTCACAAATTTGCAATTCAATCTAGCATAAAAATTGAAAAAACA
AGAGAACTTGTAAAAAATTTTATTAATGCAGAATCTTCAAAAAATATAATATTTACTTCTGGAACTACGGATGGAATTAA
CATCATCGCAAACTCATTTTTTTACTCAAAATACTTTAAAAAAAAAGATGAAATTATTCTTACAACTCTTGAACATAATA
GTAATTTGCTGCCATGGGCAAATCTTGCAAAATTAGCTAATCTAACAATTAAGTTTGCTAAATTCAATGAAATGGGAATT
ATTACCCCCGAAGAAATTGAAAAACTTATTACAGAAAAAACAAAGCTTATAAGTATTTCAGGAATAAATAATACCTTGGG
AACAATGAATGATTTAGAATCTATTGGAAAAATCGCAAAAAAATATAATATAAGTCTTTTTGTAGATGCTGCACAAATGG
CGCCGCATATAAAAATAGATGTTAAAAAAATTGGCTGTGACTTTTTGGTATTTTCTGGCCATAAAATGCTTGCTCCAACA
GGAATAGGAATTTTATACATTTCAAATAATATGGCTGAAAAACTTGATAGTCCAAAATTAGGAGGAAATACTGTAGAAGA
AATATTCATAAAAAATGAAAAAATTAAATTTAAATCACTTGATGCTCCCAATAAATTCGAATCAGGAACTCCAAATATTG
CAGGAATTATTGGGCTTAAAGAGGCAATAAAATATATCAATAATATTTCTATGGATTTCATTTTAGAACATGATCAGCAA
TTAATAGAATATGGGGTAAAAAAATTACAAGAGCTTGATGAAGTCGAATTTTTACTAAATACAAATCTTAAAAGAAATTC
AATAATATCATTTACAGTAAAAAATATTCACTCACATGATATTGAAACATATTTAGATACAATTGGAATAGCAACTAGAG
CCGGAAAAACTTGTTCTTATGTAGCATTTTTCCCAGAAAATTTAAATAAAAACCATCTTTTAAGAATTAGCTTTTATTTT
TATAATACACAAGAAGAAATTGATATTTTTATATTGGGATTAAAAAAAGTAATAAAAGAGCTTTCATAA

Upstream 100 bases:

>100_bases
ATTATTAATTAATAATCATTTTAAACTTTTTTTATGAAATAAAAAATAATGCTATAATGATTAAAAGCTAAAAATTAAAA
CAAAGGAACTTTTAAGGATT

Downstream 100 bases:

>100_bases
AAATAGAGAAAAATAAAAATCATAACAAAGTTAATCAAAAATCTTTCTTTTTGATTAACTTTAATTTTAGGATTAATAAG
CATTATTTCAATCTTTAAAA

Product: nifS protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MDFKQIKSNAEKVKFLRKDFPILNKQFDNKHIIYFDNAATSQKPKKVIYSIIEYYENYNANVHRSGHKFAIQSSIKIEKT
RELVKNFINAESSKNIIFTSGTTDGINIIANSFFYSKYFKKKDEIILTTLEHNSNLLPWANLAKLANLTIKFAKFNEMGI
ITPEEIEKLITEKTKLISISGINNTLGTMNDLESIGKIAKKYNISLFVDAAQMAPHIKIDVKKIGCDFLVFSGHKMLAPT
GIGILYISNNMAEKLDSPKLGGNTVEEIFIKNEKIKFKSLDAPNKFESGTPNIAGIIGLKEAIKYINNISMDFILEHDQQ
LIEYGVKKLQELDEVEFLLNTNLKRNSIISFTVKNIHSHDIETYLDTIGIATRAGKTCSYVAFFPENLNKNHLLRISFYF
YNTQEEIDIFILGLKKVIKELS

Sequences:

>Translated_422_residues
MDFKQIKSNAEKVKFLRKDFPILNKQFDNKHIIYFDNAATSQKPKKVIYSIIEYYENYNANVHRSGHKFAIQSSIKIEKT
RELVKNFINAESSKNIIFTSGTTDGINIIANSFFYSKYFKKKDEIILTTLEHNSNLLPWANLAKLANLTIKFAKFNEMGI
ITPEEIEKLITEKTKLISISGINNTLGTMNDLESIGKIAKKYNISLFVDAAQMAPHIKIDVKKIGCDFLVFSGHKMLAPT
GIGILYISNNMAEKLDSPKLGGNTVEEIFIKNEKIKFKSLDAPNKFESGTPNIAGIIGLKEAIKYINNISMDFILEHDQQ
LIEYGVKKLQELDEVEFLLNTNLKRNSIISFTVKNIHSHDIETYLDTIGIATRAGKTCSYVAFFPENLNKNHLLRISFYF
YNTQEEIDIFILGLKKVIKELS
>Mature_422_residues
MDFKQIKSNAEKVKFLRKDFPILNKQFDNKHIIYFDNAATSQKPKKVIYSIIEYYENYNANVHRSGHKFAIQSSIKIEKT
RELVKNFINAESSKNIIFTSGTTDGINIIANSFFYSKYFKKKDEIILTTLEHNSNLLPWANLAKLANLTIKFAKFNEMGI
ITPEEIEKLITEKTKLISISGINNTLGTMNDLESIGKIAKKYNISLFVDAAQMAPHIKIDVKKIGCDFLVFSGHKMLAPT
GIGILYISNNMAEKLDSPKLGGNTVEEIFIKNEKIKFKSLDAPNKFESGTPNIAGIIGLKEAIKYINNISMDFILEHDQQ
LIEYGVKKLQELDEVEFLLNTNLKRNSIISFTVKNIHSHDIETYLDTIGIATRAGKTCSYVAFFPENLNKNHLLRISFYF
YNTQEEIDIFILGLKKVIKELS

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine [H]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=398, Percent_Identity=23.1155778894472, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI156713448, Length=435, Percent_Identity=22.7586206896552, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1787970, Length=413, Percent_Identity=34.3825665859564, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1789175, Length=411, Percent_Identity=30.9002433090024, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=25, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25143064, Length=390, Percent_Identity=23.3333333333333, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI193211090, Length=383, Percent_Identity=25.065274151436, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17533177, Length=292, Percent_Identity=25.3424657534247, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319831, Length=402, Percent_Identity=25.1243781094527, Blast_Score=125, Evalue=8e-30,
Organism=Drosophila melanogaster, GI20129463, Length=244, Percent_Identity=28.2786885245902, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 48111; Mature: 48111

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKQIKSNAEKVKFLRKDFPILNKQFDNKHIIYFDNAATSQKPKKVIYSIIEYYENYNA
CCHHHHHCHHHHHHHHHHCCCHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
NVHRSGHKFAIQSSIKIEKTRELVKNFINAESSKNIIFTSGTTDGINIIANSFFYSKYFK
CEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHC
KKDEIILTTLEHNSNLLPWANLAKLANLTIKFAKFNEMGIITPEEIEKLITEKTKLISIS
CCCCEEEEEEECCCCCCCHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHCCEEEEEEE
GINNTLGTMNDLESIGKIAKKYNISLFVDAAQMAPHIKIDVKKIGCDFLVFSGHKMLAPT
CCCCCCCCHHHHHHHHHHHHHCCEEEEEEHHHHCCCEEEEEEECCCEEEEECCCEEECCC
GIGILYISNNMAEKLDSPKLGGNTVEEIFIKNEKIKFKSLDAPNKFESGTPNIAGIIGLK
CEEEEEEECCHHHHCCCCCCCCCCHHHHEEECCCEEEEECCCCCCCCCCCCCCEEHHHHH
EAIKYINNISMDFILEHDQQLIEYGVKKLQELDEVEFLLNTNLKRNSIISFTVKNIHSHD
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHH
IETYLDTIGIATRAGKTCSYVAFFPENLNKNHLLRISFYFYNTQEEIDIFILGLKKVIKE
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCCEEEEEEEHHHHHHH
LS
CC
>Mature Secondary Structure
MDFKQIKSNAEKVKFLRKDFPILNKQFDNKHIIYFDNAATSQKPKKVIYSIIEYYENYNA
CCHHHHHCHHHHHHHHHHCCCHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
NVHRSGHKFAIQSSIKIEKTRELVKNFINAESSKNIIFTSGTTDGINIIANSFFYSKYFK
CEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHC
KKDEIILTTLEHNSNLLPWANLAKLANLTIKFAKFNEMGIITPEEIEKLITEKTKLISIS
CCCCEEEEEEECCCCCCCHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHCCEEEEEEE
GINNTLGTMNDLESIGKIAKKYNISLFVDAAQMAPHIKIDVKKIGCDFLVFSGHKMLAPT
CCCCCCCCHHHHHHHHHHHHHCCEEEEEEHHHHCCCEEEEEEECCCEEEEECCCEEECCC
GIGILYISNNMAEKLDSPKLGGNTVEEIFIKNEKIKFKSLDAPNKFESGTPNIAGIIGLK
CEEEEEEECCHHHHCCCCCCCCCCHHHHEEECCCEEEEECCCCCCCCCCCCCCEEHHHHH
EAIKYINNISMDFILEHDQQLIEYGVKKLQELDEVEFLLNTNLKRNSIISFTVKNIHSHD
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHH
IETYLDTIGIATRAGKTCSYVAFFPENLNKNHLLRISFYFYNTQEEIDIFILGLKKVIKE
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCCEEEEEEEHHHHHHH
LS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]