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The map label for this gene is lplA

Identifier: 51596646

GI number: 51596646

Start: 2744703

End: 2745719

Strand: Reverse

Name: lplA

Synonym: YPTB2322

Alternate gene names: 51596646

Gene position: 2745719-2744703 (Counterclockwise)

Preceding gene: 51596647

Following gene: 51596645

Centisome position: 57.87

GC content: 45.43

Gene sequence:

>1017_bases
ATGTCATCCCTTCGTTTACTCATTTCTGATTCTTATGATCCTTGGTTTAATCTGGCCGTAGAAGAGTGTATTTTTCGCCA
AATGTCACCGGATCAACGGGTATTATTCTTGTGGCGCAATGCGGATACCGTGGTTATTGGCCGCGCACAGAACCCGTGGA
AAGAGTGTAATACACGACGTATGGAACAAGATGGCGTAAAGTTAGCGCGCCGTAGCAGTGGTGGCGGGGCGGTATTTCAT
GATTTAGGTAATACCTGTTTTACCTTTATGGCCGGCAAACCGGGATATGATAAAACTATTTCAACCCAAATTATTTTGAA
TGCGTTGGCATCATTAGGGATCCAGGCAACGGCCTCAGGTCGCAATGATTTAGTGGTTATTAATGGTGAAGATGAACGTA
AAGTTTCAGGTTCTGCTTATAAAGAAACCAAAGATCGTGGTTTTCATCATGGAACACTATTATTGAATGCAGATCTCAGT
CGCCTGGCTGATTATCTCAATCCAGACCCTAAAAAGTTACAGGCTAAAGGAATTACCTCGGTACGCTCCAGAGTCACTAA
TCTGGTCGAACTGTTACCGGGTATTGATCACGAAAAAATACGTACCGCTATCGAACAGGCTTTTTTTGCTTATTACGATG
AGCAGGTATCCGCAGAAGTCATCTCTCCGCAGTCGTTACCAAACCTCCCTGGCTTTACCGAGCAATTCGCCAAACAAAGT
AGTTGGGAATGGAATTTTGGCCAAGCCCCTGCATTTAGCCACGTGGTTGATACCCGCTTTACCTGGGGGGGTATTGAGTT
ACATTTTGATGTTTTGCACGGCGCAATAGATCGCTGCCAAATATTTACTGACAGCTTGAACCCCACGCCATTGGAGGCGT
TAGCGCAACGGCTACAGGGGGCAGCTTATCGCCCTGATGCTATCGATAAAATCTGTCAGCACTGGATTGATGATTTCCCT
GAATTACAAACCGAGTTACAGCAAGCCTGCCATTGGCTGGTAGAAGTATTACGCTAA

Upstream 100 bases:

>100_bases
AATTTTAGTTGACGACGACAGTCCCTCTCTTCTCGGCCTAAGGATATCATCAGCGAATATGCTATCTTAGTGGCTGTTTA
TTTTTGGGTCGGAGCCTTCT

Downstream 100 bases:

>100_bases
TCTTTCAATTACCGTGGTTATTGGCCGCGCACAGCACTCGTGGACATAGAGATGAAACCCAGAATGGAATACGCCCCTGA
ATTCGATAACAGCTATGCGC

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSSLRLLISDSYDPWFNLAVEECIFRQMSPDQRVLFLWRNADTVVIGRAQNPWKECNTRRMEQDGVKLARRSSGGGAVFH
DLGNTCFTFMAGKPGYDKTISTQIILNALASLGIQATASGRNDLVVINGEDERKVSGSAYKETKDRGFHHGTLLLNADLS
RLADYLNPDPKKLQAKGITSVRSRVTNLVELLPGIDHEKIRTAIEQAFFAYYDEQVSAEVISPQSLPNLPGFTEQFAKQS
SWEWNFGQAPAFSHVVDTRFTWGGIELHFDVLHGAIDRCQIFTDSLNPTPLEALAQRLQGAAYRPDAIDKICQHWIDDFP
ELQTELQQACHWLVEVLR

Sequences:

>Translated_338_residues
MSSLRLLISDSYDPWFNLAVEECIFRQMSPDQRVLFLWRNADTVVIGRAQNPWKECNTRRMEQDGVKLARRSSGGGAVFH
DLGNTCFTFMAGKPGYDKTISTQIILNALASLGIQATASGRNDLVVINGEDERKVSGSAYKETKDRGFHHGTLLLNADLS
RLADYLNPDPKKLQAKGITSVRSRVTNLVELLPGIDHEKIRTAIEQAFFAYYDEQVSAEVISPQSLPNLPGFTEQFAKQS
SWEWNFGQAPAFSHVVDTRFTWGGIELHFDVLHGAIDRCQIFTDSLNPTPLEALAQRLQGAAYRPDAIDKICQHWIDDFP
ELQTELQQACHWLVEVLR
>Mature_337_residues
SSLRLLISDSYDPWFNLAVEECIFRQMSPDQRVLFLWRNADTVVIGRAQNPWKECNTRRMEQDGVKLARRSSGGGAVFHD
LGNTCFTFMAGKPGYDKTISTQIILNALASLGIQATASGRNDLVVINGEDERKVSGSAYKETKDRGFHHGTLLLNADLSR
LADYLNPDPKKLQAKGITSVRSRVTNLVELLPGIDHEKIRTAIEQAFFAYYDEQVSAEVISPQSLPNLPGFTEQFAKQSS
WEWNFGQAPAFSHVVDTRFTWGGIELHFDVLHGAIDRCQIFTDSLNPTPLEALAQRLQGAAYRPDAIDKICQHWIDDFPE
LQTELQQACHWLVEVLR

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=285, Percent_Identity=34.7368421052632, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI21729884, Length=285, Percent_Identity=34.7368421052632, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI21729882, Length=285, Percent_Identity=34.7368421052632, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI21729880, Length=285, Percent_Identity=34.7368421052632, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI21729878, Length=285, Percent_Identity=34.7368421052632, Blast_Score=171, Evalue=7e-43,
Organism=Escherichia coli, GI1790846, Length=338, Percent_Identity=75.4437869822485, Blast_Score=541, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71981909, Length=254, Percent_Identity=30.7086614173228, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322415, Length=194, Percent_Identity=32.4742268041237, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28573464, Length=262, Percent_Identity=34.7328244274809, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38055; Mature: 37924

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLRLLISDSYDPWFNLAVEECIFRQMSPDQRVLFLWRNADTVVIGRAQNPWKECNTRR
CCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCHHHHHHHH
MEQDGVKLARRSSGGGAVFHDLGNTCFTFMAGKPGYDKTISTQIILNALASLGIQATASG
HHHHHHHHHHCCCCCCCHHHHCCCHHEEHCCCCCCCCCHHHHHHHHHHHHHCCCEEECCC
RNDLVVINGEDERKVSGSAYKETKDRGFHHGTLLLNADLSRLADYLNPDPKKLQAKGITS
CCCEEEECCCCCCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHH
VRSRVTNLVELLPGIDHEKIRTAIEQAFFAYYDEQVSAEVISPQSLPNLPGFTEQFAKQS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
SWEWNFGQAPAFSHVVDTRFTWGGIELHFDVLHGAIDRCQIFTDSLNPTPLEALAQRLQG
CCCCCCCCCCCHHHHHHCCEECCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
AAYRPDAIDKICQHWIDDFPELQTELQQACHWLVEVLR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SSLRLLISDSYDPWFNLAVEECIFRQMSPDQRVLFLWRNADTVVIGRAQNPWKECNTRR
CCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCHHHHHHHH
MEQDGVKLARRSSGGGAVFHDLGNTCFTFMAGKPGYDKTISTQIILNALASLGIQATASG
HHHHHHHHHHCCCCCCCHHHHCCCHHEEHCCCCCCCCCHHHHHHHHHHHHHCCCEEECCC
RNDLVVINGEDERKVSGSAYKETKDRGFHHGTLLLNADLSRLADYLNPDPKKLQAKGITS
CCCEEEECCCCCCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHH
VRSRVTNLVELLPGIDHEKIRTAIEQAFFAYYDEQVSAEVISPQSLPNLPGFTEQFAKQS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
SWEWNFGQAPAFSHVVDTRFTWGGIELHFDVLHGAIDRCQIFTDSLNPTPLEALAQRLQG
CCCCCCCCCCCHHHHHHCCEECCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
AAYRPDAIDKICQHWIDDFPELQTELQQACHWLVEVLR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA