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The map label for this gene is purU

Identifier: 51596423

GI number: 51596423

Start: 2469767

End: 2470615

Strand: Reverse

Name: purU

Synonym: YPTB2097

Alternate gene names: 51596423

Gene position: 2470615-2469767 (Counterclockwise)

Preceding gene: 51596424

Following gene: 51596420

Centisome position: 52.07

GC content: 42.87

Gene sequence:

>849_bases
ATGCCACACCAGAATGTACAAAAGAAAGTATTGCGCACTATTTGCCCTGACGCTAAAGGTTTGATCGCAAAGATCACTAA
TATCTGCTACAAGCACCAATTGAATATCGTACAAAATAATGAATTTGTTGATCACCTGACTGGTCGCTTCTTTATGCGCA
CCGAGCTTGAGGGGATTTTTAACGATACGACATTACTGGCAGATCTTGACGATGCATTACCTGAAGGCACTAATCGCGAG
TTACACGTTGCTGGGCGCCGCCGCATCATCATTATGGTAACGAAAGAAGCACATTGCCTTGGCGACTTATTAATGAAGAG
TGCTTATGGTGGCTTAGATGTAGAAATTGCGGCAGTCATTGGTAACCATGATGCATTGCAAAATTTAGTCGAGCGTTTTG
ATATCCCATTCCATCTGGTCAGCCATGAAGGCCTCACCCGTGAGCAGCATGATCAACAGTTGATTGAGAAAATTGAACAA
TATCAACCAGATTATGTTGTTCTGGCTAAATATATGCGGGTGCTAACACCGGCCTTTGTGCAACGTTTCCCTTATCAAAT
TATTAATATTCATCATTCATTTTTGCCTGCATTTATCGGTGCTCGGCCATACCACCAAGCGTATGAACGCGGAGTGAAGA
TTATTGGCGCTACTGCTCATTACGTAAATGATAGCTTGGATGAAGGCCCAATCATCATGCAGGACGTGATCAATGTGGAT
CATTCTTATACTGCTGAAGATATGATGCGTGCTGGTCGTGATGTAGAAAAGAATGTTCTCAGTAGTGCGCTATACCGTGT
TCTCGCTCAACGTGTTTTTGTTTACGGTAACCGCACTGTTATCCTATAA

Upstream 100 bases:

>100_bases
AATGCTGTGGCCGTTAATTAAACTATTTCTTTATTGTTACTCCTAAAATATCAGACACTTACACAACATGAAATAATGTT
AAGACAGGATTTACCACCTT

Downstream 100 bases:

>100_bases
AGTGATGTTTTTTGTCTAGATATCCATCTTCACGGACAAAAAAGCACCGAACGAATTATTTTTTTCATTTCAAGTCTTTA
CAGGGGCGCTTCATTTGATA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPHQNVQKKVLRTICPDAKGLIAKITNICYKHQLNIVQNNEFVDHLTGRFFMRTELEGIFNDTTLLADLDDALPEGTNRE
LHVAGRRRIIIMVTKEAHCLGDLLMKSAYGGLDVEIAAVIGNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIEQ
YQPDYVVLAKYMRVLTPAFVQRFPYQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDSLDEGPIIMQDVINVD
HSYTAEDMMRAGRDVEKNVLSSALYRVLAQRVFVYGNRTVIL

Sequences:

>Translated_282_residues
MPHQNVQKKVLRTICPDAKGLIAKITNICYKHQLNIVQNNEFVDHLTGRFFMRTELEGIFNDTTLLADLDDALPEGTNRE
LHVAGRRRIIIMVTKEAHCLGDLLMKSAYGGLDVEIAAVIGNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIEQ
YQPDYVVLAKYMRVLTPAFVQRFPYQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDSLDEGPIIMQDVINVD
HSYTAEDMMRAGRDVEKNVLSSALYRVLAQRVFVYGNRTVIL
>Mature_281_residues
PHQNVQKKVLRTICPDAKGLIAKITNICYKHQLNIVQNNEFVDHLTGRFFMRTELEGIFNDTTLLADLDDALPEGTNREL
HVAGRRRIIIMVTKEAHCLGDLLMKSAYGGLDVEIAAVIGNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIEQY
QPDYVVLAKYMRVLTPAFVQRFPYQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDSLDEGPIIMQDVINVDH
SYTAEDMMRAGRDVEKNVLSSALYRVLAQRVFVYGNRTVIL

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=29.4736842105263, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=29.4736842105263, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=29.4736842105263, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787483, Length=279, Percent_Identity=85.3046594982079, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI1788846, Length=103, Percent_Identity=41.747572815534, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=187, Percent_Identity=31.0160427807487, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24582400, Length=159, Percent_Identity=29.559748427673, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32204; Mature: 32072

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHQNVQKKVLRTICPDAKGLIAKITNICYKHQLNIVQNNEFVDHLTGRFFMRTELEGIF
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHH
NDTTLLADLDDALPEGTNRELHVAGRRRIIIMVTKEAHCLGDLLMKSAYGGLDVEIAAVI
CCCHHEEECHHHCCCCCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEC
GNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIEQYQPDYVVLAKYMRVLTPAFV
CCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QRFPYQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDSLDEGPIIMQDVINVD
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHHCCC
HSYTAEDMMRAGRDVEKNVLSSALYRVLAQRVFVYGNRTVIL
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure 
PHQNVQKKVLRTICPDAKGLIAKITNICYKHQLNIVQNNEFVDHLTGRFFMRTELEGIF
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHH
NDTTLLADLDDALPEGTNRELHVAGRRRIIIMVTKEAHCLGDLLMKSAYGGLDVEIAAVI
CCCHHEEECHHHCCCCCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEC
GNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIEQYQPDYVVLAKYMRVLTPAFV
CCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QRFPYQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDSLDEGPIIMQDVINVD
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHHCCC
HSYTAEDMMRAGRDVEKNVLSSALYRVLAQRVFVYGNRTVIL
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]