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The map label for this gene is ppnK

Identifier: 51595484

GI number: 51595484

Start: 1363647

End: 1364528

Strand: Reverse

Name: ppnK

Synonym: YPTB1140

Alternate gene names: 51595484

Gene position: 1364528-1363647 (Counterclockwise)

Preceding gene: 51595486

Following gene: 51595483

Centisome position: 28.76

GC content: 47.05

Gene sequence:

>882_bases
ATGAATAATAGAAGATTCGATTGTATTGGTATTGTCGGTCACCCGCGACACCCGGCTGCACTTGCCACCCATGAAATACT
CTACCACTGGCTAAAAGCCAGAGGCTACGCCGTTATGGTTGAACAACAGATTGCTCATGACCTCAATTTAACCGATGCCA
TAACAGGGAGTTTGGCAGATATTGGCCAAAAAGCAGATTTGGCTGTTGTTGTCGGCGGTGATGGCAACATGCTCGGTGCT
GCACGGGTACTTGCCCGTTATGATATAAAAGTGATTGGTGTCAACCGAGGTAATCTGGGTTTCTTGACCGATTTAGACCC
GGATAATGCTCTGCAGCAGCTTTCAGACGTTTTGGAAGGCGAGTACCTGAGTGAACAACGCTTCCTGCTAGAAACGCATG
TACGACGTACAAATCAACAAAGCCGTATCAGTACCGCAATCAATGAAGTGGTTTTGCATCCAGGCAAAGTCGCCCATATG
ATCGAATTCGAAGTGTATATTGATGACCGCTTCGCCTTTTCTCAACGCTCTGATGGCTTGATCATCGCAACCCCGACCGG
TTCTACCGCCTATTCACTTTCCGCAGGCGGCCCAATCTTGACACCAACTCTGGATGCCATCGTATTGGTACCAATGTTTC
CACACACTCTTACTGCGCGCCCATTGGTTATCAGCAGCACTAGCACCATCCGACTGAAGTTTTCGCACATTACCAGCGAC
CTGGAAATCAGTTGCGATAGCCAGATAGCCCTACCCATTCAGGAAGGGGAAGAGGTTTTAATCCGGCGCAGTGATTTTCA
CCTCAATCTTATTCATCCAAAGGACTATAGCTATTTCAATACGTTAAGCACCAAATTGGGTTGGTCAAAAAAATTATTCT
AA

Upstream 100 bases:

>100_bases
AATCTCGCTACTTGGCTTATTATGGGGATCAAAACCGGGGATTCAAGGGAACCGGTCATAATGTGGGGTAAAAGACACTC
CATTAAGGAAAATCACGGCA

Downstream 100 bases:

>100_bases
AAATCACCTCAGCTACTTTACTGTATATAAAACCAGGCTATACTGTATTCAAATACAGACATGTGTTTATGTACAGGGGG
TTTACCATGTTGGTCCAATT

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNNRRFDCIGIVGHPRHPAALATHEILYHWLKARGYAVMVEQQIAHDLNLTDAITGSLADIGQKADLAVVVGGDGNMLGA
ARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLSDVLEGEYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVAHM
IEFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGPILTPTLDAIVLVPMFPHTLTARPLVISSTSTIRLKFSHITSD
LEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF

Sequences:

>Translated_293_residues
MNNRRFDCIGIVGHPRHPAALATHEILYHWLKARGYAVMVEQQIAHDLNLTDAITGSLADIGQKADLAVVVGGDGNMLGA
ARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLSDVLEGEYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVAHM
IEFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGPILTPTLDAIVLVPMFPHTLTARPLVISSTSTIRLKFSHITSD
LEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF
>Mature_293_residues
MNNRRFDCIGIVGHPRHPAALATHEILYHWLKARGYAVMVEQQIAHDLNLTDAITGSLADIGQKADLAVVVGGDGNMLGA
ARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLSDVLEGEYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVAHM
IEFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGPILTPTLDAIVLVPMFPHTLTARPLVISSTSTIRLKFSHITSD
LEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=259, Percent_Identity=27.027027027027, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1788968, Length=293, Percent_Identity=82.5938566552901, Blast_Score=503, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6325068, Length=215, Percent_Identity=33.4883720930233, Blast_Score=125, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6320794, Length=243, Percent_Identity=28.8065843621399, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322509, Length=240, Percent_Identity=29.5833333333333, Blast_Score=115, Evalue=6e-27,
Organism=Drosophila melanogaster, GI28573832, Length=305, Percent_Identity=27.5409836065574, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI28573830, Length=307, Percent_Identity=27.3615635179153, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28573826, Length=307, Percent_Identity=27.3615635179153, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28573828, Length=307, Percent_Identity=27.3615635179153, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI161077047, Length=305, Percent_Identity=27.5409836065574, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20129957, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653422, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281363323, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281363321, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653424, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 32413; Mature: 32413

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRRFDCIGIVGHPRHPAALATHEILYHWLKARGYAVMVEQQIAHDLNLTDAITGSLAD
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEHHHHCCCCCHHHHCCCHHH
IGQKADLAVVVGGDGNMLGAARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLSDVLEG
CCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHCC
EYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVAHMIEFEVYIDDRFAFSQRSDGL
CHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECEEEECCCCCCE
IIATPTGSTAYSLSAGGPILTPTLDAIVLVPMFPHTLTARPLVISSTSTIRLKFSHITSD
EEEECCCCCEEEECCCCCEECCCCCEEEEEECCCCCCCCCCEEEECCCEEEEEEEECCCC
LEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF
EEEECCCEEEEECCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNNRRFDCIGIVGHPRHPAALATHEILYHWLKARGYAVMVEQQIAHDLNLTDAITGSLAD
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEHHHHCCCCCHHHHCCCHHH
IGQKADLAVVVGGDGNMLGAARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLSDVLEG
CCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHCC
EYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVAHMIEFEVYIDDRFAFSQRSDGL
CHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECEEEECCCCCCE
IIATPTGSTAYSLSAGGPILTPTLDAIVLVPMFPHTLTARPLVISSTSTIRLKFSHITSD
EEEECCCCCEEEECCCCCEECCCCCEEEEEECCCCCCCCCCEEEECCCEEEEEEEECCCC
LEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSYFNTLSTKLGWSKKLF
EEEECCCEEEEECCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]