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The map label for this gene is gatA [H]

Identifier: 51595212

GI number: 51595212

Start: 1038943

End: 1040340

Strand: Reverse

Name: gatA [H]

Synonym: YPTB0862

Alternate gene names: 51595212

Gene position: 1040340-1038943 (Counterclockwise)

Preceding gene: 51595213

Following gene: 51595211

Centisome position: 21.93

GC content: 54.79

Gene sequence:

>1398_bases
ATGAAGCCTGCCTCATCACTCACTATCAGCGAGATCCAACAAGCCCTGCACAAGAGCGAATTATCCGCACAAGCGATCGC
ACAGCAGACGTTGGACGCCATTACCGTCGCCAATCCGCTCATCAACGCTTATACCCATATCACCGCTGACCGTATGCTTA
GCGAAGCTTGTCGGCTAGATACGCTACGCGCCAAAGGCCAGCCGCTGCCAGCACTGGCGGCAGTCCCTTATGCGGTCAAG
AATCTGTTCGATGTTGCCGGAGAAACAACCCTATCAGGTGCCAGCTTATTTTGTGATCAAGCCCCCGCCAAACAGGATGC
CTGGGCCATTTCATGCCTGAGCCACCAAGGAGCCATGCTCTCCGGCATGTTAAATATGGATGCCTATGCCTACGGTTTTA
CTACCGAGAACAGCCATTATGGTGCCACCCATAATCCACACGATCTGAACCGCATTGCAGGGGGATCTTCTGGCGGATCT
GCGGCAGCGGTCGCCGCAGGGCTCGTGCACTTTTCTCTCGGCAGTGATACTAACGGTTCGATCCGGGTTCCCTCCTCACT
GTGTGGTATTTTTGGGCTGAAACCAACCTTTGGCCGCCTGTCACGCCGCGGCACCCACCCATTTGTCGCCAGTCTGGACC
ATATCGGCCCAATGGCACGCTGTACGCAAGATTTGGCGGTGGTTTATGACGTGATGCAAGGCATCGATACTCAGGATAAT
TTTCAAGCCGATCAACCCGTCACGCCGTGCCAAAATTTACTGACGCGTGGTCAGCAAGGGTTAAGAAGCGCGGTGCTGGG
CGGTTACTTTCATCAATGGAGTGATGATGACGCCAAAGCCGCAGTGCTGGCGGTCGCAAAGGCGCTTGAGGCTAACGCGG
AGGTCGAGATGCCGCAGAGTGATTTAGCCCGGTCAGCGGCATTTATTATCAGTGCCAGCGAGGGCGGCAATCAATATCTG
CCCGCCTTGAGGAAATCACCTCAACGTTTTGAAACCCTATCACGGGAGCGGTTATTAGCCGGAGCCATGCTACCGGCAAC
TTGGTACGTACAGGCCCAGCGTTTCCGTCATCATTTTCGCCAACACATTTTGCCGCTGTTCGAGCACTGGGATATTTTGA
TTGCCCCAGCAACGCCATGCAGTGCCACGCTTATCGGCCAGGACACGATGAGGATTAATGATATGGACCTACCGATCCGC
GCCAGTATGGGCATATTAACCCAGCCAATCTCCTTCCTGGGTCTGCCAGTCGTGAGCGTACCCTTGAAAACCACCCGCGG
CTTACCAATTGGCATTCAGTTGATTGCCCCACCATGGCGAGAAGATTTATGCCTGCGCGCGGCTTATGCGCTAGAGCAAC
AAGGGTTGGTCAGCGCGCCGGTGAGTACTGTTATTTGA

Upstream 100 bases:

>100_bases
CGGCATTGCAGCAACAATTTGAACACATTGCCGCTCTGGCCGCACCGCTGATGGCCCTACCTTTAGATGAACGGCAAGAG
ATCGCGGGGGTATACCGGTT

Downstream 100 bases:

>100_bases
TAGGACCGTTATTTGATAGGACCGCTATTTGATAGGTCCGTTATTTGATAGGACCGTGCTTGATAAAGATAGCCATTTGG
CGAGAACCGTCTCCGATGAG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKPASSLTISEIQQALHKSELSAQAIAQQTLDAITVANPLINAYTHITADRMLSEACRLDTLRAKGQPLPALAAVPYAVK
NLFDVAGETTLSGASLFCDQAPAKQDAWAISCLSHQGAMLSGMLNMDAYAYGFTTENSHYGATHNPHDLNRIAGGSSGGS
AAAVAAGLVHFSLGSDTNGSIRVPSSLCGIFGLKPTFGRLSRRGTHPFVASLDHIGPMARCTQDLAVVYDVMQGIDTQDN
FQADQPVTPCQNLLTRGQQGLRSAVLGGYFHQWSDDDAKAAVLAVAKALEANAEVEMPQSDLARSAAFIISASEGGNQYL
PALRKSPQRFETLSRERLLAGAMLPATWYVQAQRFRHHFRQHILPLFEHWDILIAPATPCSATLIGQDTMRINDMDLPIR
ASMGILTQPISFLGLPVVSVPLKTTRGLPIGIQLIAPPWREDLCLRAAYALEQQGLVSAPVSTVI

Sequences:

>Translated_465_residues
MKPASSLTISEIQQALHKSELSAQAIAQQTLDAITVANPLINAYTHITADRMLSEACRLDTLRAKGQPLPALAAVPYAVK
NLFDVAGETTLSGASLFCDQAPAKQDAWAISCLSHQGAMLSGMLNMDAYAYGFTTENSHYGATHNPHDLNRIAGGSSGGS
AAAVAAGLVHFSLGSDTNGSIRVPSSLCGIFGLKPTFGRLSRRGTHPFVASLDHIGPMARCTQDLAVVYDVMQGIDTQDN
FQADQPVTPCQNLLTRGQQGLRSAVLGGYFHQWSDDDAKAAVLAVAKALEANAEVEMPQSDLARSAAFIISASEGGNQYL
PALRKSPQRFETLSRERLLAGAMLPATWYVQAQRFRHHFRQHILPLFEHWDILIAPATPCSATLIGQDTMRINDMDLPIR
ASMGILTQPISFLGLPVVSVPLKTTRGLPIGIQLIAPPWREDLCLRAAYALEQQGLVSAPVSTVI
>Mature_465_residues
MKPASSLTISEIQQALHKSELSAQAIAQQTLDAITVANPLINAYTHITADRMLSEACRLDTLRAKGQPLPALAAVPYAVK
NLFDVAGETTLSGASLFCDQAPAKQDAWAISCLSHQGAMLSGMLNMDAYAYGFTTENSHYGATHNPHDLNRIAGGSSGGS
AAAVAAGLVHFSLGSDTNGSIRVPSSLCGIFGLKPTFGRLSRRGTHPFVASLDHIGPMARCTQDLAVVYDVMQGIDTQDN
FQADQPVTPCQNLLTRGQQGLRSAVLGGYFHQWSDDDAKAAVLAVAKALEANAEVEMPQSDLARSAAFIISASEGGNQYL
PALRKSPQRFETLSRERLLAGAMLPATWYVQAQRFRHHFRQHILPLFEHWDILIAPATPCSATLIGQDTMRINDMDLPIR
ASMGILTQPISFLGLPVVSVPLKTTRGLPIGIQLIAPPWREDLCLRAAYALEQQGLVSAPVSTVI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=514, Percent_Identity=28.0155642023346, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI195972892, Length=244, Percent_Identity=31.1475409836066, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI166795287, Length=503, Percent_Identity=26.2425447316103, Blast_Score=96, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=444, Percent_Identity=26.5765765765766, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=294, Percent_Identity=29.2517006802721, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17537465, Length=504, Percent_Identity=25.9920634920635, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=258, Percent_Identity=26.3565891472868, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=464, Percent_Identity=23.4913793103448, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=170, Percent_Identity=34.7058823529412, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=170, Percent_Identity=34.7058823529412, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=450, Percent_Identity=27.5555555555556, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323950, Length=466, Percent_Identity=24.4635193133047, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320448, Length=230, Percent_Identity=32.1739130434783, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648113, Length=494, Percent_Identity=31.3765182186235, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=25.4509018036072, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24652985, Length=485, Percent_Identity=28.2474226804124, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922090, Length=485, Percent_Identity=28.2474226804124, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24652981, Length=485, Percent_Identity=28.2474226804124, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24652983, Length=485, Percent_Identity=28.2474226804124, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078093, Length=499, Percent_Identity=27.0541082164329, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI45550774, Length=498, Percent_Identity=25.9036144578313, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648435, Length=495, Percent_Identity=25.6565656565657, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648437, Length=495, Percent_Identity=25.6565656565657, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356731, Length=476, Percent_Identity=25.4201680672269, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648441, Length=437, Percent_Identity=26.3157894736842, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648439, Length=437, Percent_Identity=26.3157894736842, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49888; Mature: 49888

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPASSLTISEIQQALHKSELSAQAIAQQTLDAITVANPLINAYTHITADRMLSEACRLD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLRAKGQPLPALAAVPYAVKNLFDVAGETTLSGASLFCDQAPAKQDAWAISCLSHQGAML
HHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHHCCCHHH
SGMLNMDAYAYGFTTENSHYGATHNPHDLNRIAGGSSGGSAAAVAAGLVHFSLGSDTNGS
HHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHEECCCCCCCC
IRVPSSLCGIFGLKPTFGRLSRRGTHPFVASLDHIGPMARCTQDLAVVYDVMQGIDTQDN
EECCHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
FQADQPVTPCQNLLTRGQQGLRSAVLGGYFHQWSDDDAKAAVLAVAKALEANAEVEMPQS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHH
DLARSAAFIISASEGGNQYLPALRKSPQRFETLSRERLLAGAMLPATWYVQAQRFRHHFR
HHHCCEEEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QHILPLFEHWDILIAPATPCSATLIGQDTMRINDMDLPIRASMGILTQPISFLGLPVVSV
HHHHHHHHCCCEEEECCCCCCEEEECCCCEEECCCCCCHHHCCCHHHCCHHHHCCCCEEE
PLKTTRGLPIGIQLIAPPWREDLCLRAAYALEQQGLVSAPVSTVI
CCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHCC
>Mature Secondary Structure
MKPASSLTISEIQQALHKSELSAQAIAQQTLDAITVANPLINAYTHITADRMLSEACRLD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLRAKGQPLPALAAVPYAVKNLFDVAGETTLSGASLFCDQAPAKQDAWAISCLSHQGAML
HHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHHCCCHHH
SGMLNMDAYAYGFTTENSHYGATHNPHDLNRIAGGSSGGSAAAVAAGLVHFSLGSDTNGS
HHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHEECCCCCCCC
IRVPSSLCGIFGLKPTFGRLSRRGTHPFVASLDHIGPMARCTQDLAVVYDVMQGIDTQDN
EECCHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
FQADQPVTPCQNLLTRGQQGLRSAVLGGYFHQWSDDDAKAAVLAVAKALEANAEVEMPQS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHH
DLARSAAFIISASEGGNQYLPALRKSPQRFETLSRERLLAGAMLPATWYVQAQRFRHHFR
HHHCCEEEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QHILPLFEHWDILIAPATPCSATLIGQDTMRINDMDLPIRASMGILTQPISFLGLPVVSV
HHHHHHHHCCCEEEECCCCCCEEEECCCCEEECCCCCCHHHCCCHHHCCHHHHCCCCEEE
PLKTTRGLPIGIQLIAPPWREDLCLRAAYALEQQGLVSAPVSTVI
CCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA