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The map label for this gene is yadB

Identifier: 51595083

GI number: 51595083

Start: 879166

End: 880161

Strand: Reverse

Name: yadB

Synonym: YPTB0733

Alternate gene names: 51595083

Gene position: 880161-879166 (Counterclockwise)

Preceding gene: 51595084

Following gene: 161760588

Centisome position: 18.55

GC content: 50.5

Gene sequence:

>996_bases
GTGGTTCAACAAGCGGTCATTCAACGATCAGCTAATCAGCAGTTGTCTAATCAACGATCAGCTAACCAACGAGCAACGAA
TCAACCAACAGAATATGTTGGCCGTTTTGCACCTTCGCCATCAGGGGATTTGCATTTTGGTTCACTGATTGCCGCACTCG
GGAGTTATCTGCAAGCCCGTGCTCAAGGCGGGAAGTGGTTAGTTCGTATTGAAGATATTGATCCTCCCCGTGAAGTTCCT
GGCGCGGCCTCACGTATTTTAGCGGCCTTAGAGCATTATGGTCTCCATTGGGATGGCCCGGTTATCTATCAATCACAGCG
CCATGAAGCGTATCGGGCCACGCTCAACTGGCTGGAACAGCAGGGATTAAGCTATTACTGCACCTGCACCCGTAGCCGCA
TCCACCAGCTCGGCGGGTTCTACGACGGCTACTGCCGTGATCGTCATTTACCCGCTAGCGGCGCGGCTATCCGTTTGCGG
CAGACTCAGCCGGTTTACGCTTTTTATGATAAATTACTCGGCGAACTGCATGCTCACCCTGCGTTGGCACAAGAAGATTT
TATTATCCGTCGCCGTGACGGTCTGTTCGCTTATAATCTGGCGGTGGTGGTGGATGATGCTTTTCAGGGCGTGACCGAAA
TTGTACGAGGTGCTGATTTGATTGAGCCGACGGTGCGCCAGATAGCGCTCTATCAGCAGTTGCAACACCCTGTCCCCAGC
TATATACACTTGCCGTTGGCGCTGAATAATCAGGGGAATAAGCTCTCCAAGCAGAATCATGCTCCTCCCTTGCCTAATGG
CGATCCACGTCCTATTTTAATCGACGCATTAAAGTTTTTACGCCAACCGCTACCGGAATACTGGCAAGATCTTGATTTGT
ATTTATTATTACGTTATGCCGTTGAGCATTGGACGTTGGTGTCAATCCCACTCCAGGGGGCAATAACCCCACAGAAAACA
CAACGGCATTCTCAAAGTAAGCATGGTGAGCTATGA

Upstream 100 bases:

>100_bases
ATTCGGTTCTCAGCCATTCGCTGCTGCTCAACTATTTTCTGCTGCTCAACCACGTGCTTCCCAATTTCCCAGCGCCCAGC
TTCCCGAGACTCAACAGCCT

Downstream 100 bases:

>100_bases
TTAGCCGCTGTTTTTCTGTCGTTCTGTTATTTATTAGCCACTATCGAGGTGTACCATTTTTACCCGAGTAGCCAATTTCT
GCCGTAAGGTGCTGATCCGC

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glu-Q-RSs

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MVQQAVIQRSANQQLSNQRSANQRATNQPTEYVGRFAPSPSGDLHFGSLIAALGSYLQARAQGGKWLVRIEDIDPPREVP
GAASRILAALEHYGLHWDGPVIYQSQRHEAYRATLNWLEQQGLSYYCTCTRSRIHQLGGFYDGYCRDRHLPASGAAIRLR
QTQPVYAFYDKLLGELHAHPALAQEDFIIRRRDGLFAYNLAVVVDDAFQGVTEIVRGADLIEPTVRQIALYQQLQHPVPS
YIHLPLALNNQGNKLSKQNHAPPLPNGDPRPILIDALKFLRQPLPEYWQDLDLYLLLRYAVEHWTLVSIPLQGAITPQKT
QRHSQSKHGEL

Sequences:

>Translated_331_residues
MVQQAVIQRSANQQLSNQRSANQRATNQPTEYVGRFAPSPSGDLHFGSLIAALGSYLQARAQGGKWLVRIEDIDPPREVP
GAASRILAALEHYGLHWDGPVIYQSQRHEAYRATLNWLEQQGLSYYCTCTRSRIHQLGGFYDGYCRDRHLPASGAAIRLR
QTQPVYAFYDKLLGELHAHPALAQEDFIIRRRDGLFAYNLAVVVDDAFQGVTEIVRGADLIEPTVRQIALYQQLQHPVPS
YIHLPLALNNQGNKLSKQNHAPPLPNGDPRPILIDALKFLRQPLPEYWQDLDLYLLLRYAVEHWTLVSIPLQGAITPQKT
QRHSQSKHGEL
>Mature_331_residues
MVQQAVIQRSANQQLSNQRSANQRATNQPTEYVGRFAPSPSGDLHFGSLIAALGSYLQARAQGGKWLVRIEDIDPPREVP
GAASRILAALEHYGLHWDGPVIYQSQRHEAYRATLNWLEQQGLSYYCTCTRSRIHQLGGFYDGYCRDRHLPASGAAIRLR
QTQPVYAFYDKLLGELHAHPALAQEDFIIRRRDGLFAYNLAVVVDDAFQGVTEIVRGADLIEPTVRQIALYQQLQHPVPS
YIHLPLALNNQGNKLSKQNHAPPLPNGDPRPILIDALKFLRQPLPEYWQDLDLYLLLRYAVEHWTLVSIPLQGAITPQKT
QRHSQSKHGEL

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily

Homologues:

Organism=Homo sapiens, GI134288884, Length=249, Percent_Identity=36.9477911646586, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI226510925, Length=281, Percent_Identity=70.4626334519573, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1788743, Length=239, Percent_Identity=35.5648535564854, Blast_Score=147, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI71993394, Length=251, Percent_Identity=31.0756972111554, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324540, Length=269, Percent_Identity=32.3420074349442, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24665321, Length=252, Percent_Identity=35.3174603174603, Blast_Score=134, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLUQ_YERPS (Q66EG0)

Other databases:

- EMBL:   BX936398
- RefSeq:   YP_069274.1
- ProteinModelPortal:   Q66EG0
- SMR:   Q66EG0
- GeneID:   2956923
- GenomeReviews:   BX936398_GR
- KEGG:   yps:YPTB0733
- NMPDR:   fig|273123.1.peg.858
- HOGENOM:   HBG628189
- OMA:   IIKRSDG
- ProtClustDB:   PRK05710
- BioCyc:   YPSE273123:YPTB0733-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01428
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR03838

Pfam domain/function: PF00749 tRNA-synt_1c

EC number: NA

Molecular weight: Translated: 37429; Mature: 37429

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 71-71 BINDING 198-198 BINDING 216-216 BINDING 257-257

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQQAVIQRSANQQLSNQRSANQRATNQPTEYVGRFAPSPSGDLHFGSLIAALGSYLQAR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
AQGGKWLVRIEDIDPPREVPGAASRILAALEHYGLHWDGPVIYQSQRHEAYRATLNWLEQ
HCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHH
QGLSYYCTCTRSRIHQLGGFYDGYCRDRHLPASGAAIRLRQTQPVYAFYDKLLGELHAHP
CCCEEEEEHHHHHHHHHCCCCCCHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
ALAQEDFIIRRRDGLFAYNLAVVVDDAFQGVTEIVRGADLIEPTVRQIALYQQLQHPVPS
CCCCCCCEEEECCCCEEEEHHHEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
YIHLPLALNNQGNKLSKQNHAPPLPNGDPRPILIDALKFLRQPLPEYWQDLDLYLLLRYA
EEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEHWTLVSIPLQGAITPQKTQRHSQSKHGEL
HHHCEEEEEECCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MVQQAVIQRSANQQLSNQRSANQRATNQPTEYVGRFAPSPSGDLHFGSLIAALGSYLQAR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
AQGGKWLVRIEDIDPPREVPGAASRILAALEHYGLHWDGPVIYQSQRHEAYRATLNWLEQ
HCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHH
QGLSYYCTCTRSRIHQLGGFYDGYCRDRHLPASGAAIRLRQTQPVYAFYDKLLGELHAHP
CCCEEEEEHHHHHHHHHCCCCCCHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
ALAQEDFIIRRRDGLFAYNLAVVVDDAFQGVTEIVRGADLIEPTVRQIALYQQLQHPVPS
CCCCCCCEEEECCCCEEEEHHHEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
YIHLPLALNNQGNKLSKQNHAPPLPNGDPRPILIDALKFLRQPLPEYWQDLDLYLLLRYA
EEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEHWTLVSIPLQGAITPQKTQRHSQSKHGEL
HHHCEEEEEECCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA