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The map label for this gene is pcnB

Identifier: 161760588

GI number: 161760588

Start: 877611

End: 879077

Strand: Reverse

Name: pcnB

Synonym: YPTB0732

Alternate gene names: 161760588

Gene position: 879077-877611 (Counterclockwise)

Preceding gene: 51595083

Following gene: 51595081

Centisome position: 18.53

GC content: 52.56

Gene sequence:

>1467_bases
GTGCTGATCCGCGAAGACAAAACGGTCCGTGATGACAAGGCCCGTAAAGATAAAGTGCCCGGTGAAGATAACGTGGCCCG
CAAAGAGAGAAGGCCCGCCAGAGCACATACTGGCCGCAAGGGTCACGCGGTGTCGTCTTCGGAACAGCGCCAAATGGCGA
TTATTCCGCGGGATCAGCACAATATCTCCCGCAGAGATATCAGTGACAATGCACTGAAGGTTCTCTATCGCCTGAATAAA
TCAGGTTACGAAGCTTATCTGGTGGGCGGCGGTGTCCGCGACCTGCTGCTGGGCAGAAAACCCAAAGATTTCGATATCAC
CACCAGCGCCACCCCTGAACAGGTGCGAAAACTGTTCCGTAACTGTCGTTTGGTTGGTCGCCGCTTCCGTTTGGCTCATG
TGATGTTTGGCCCTGAAATTATTGAAGTAGCGACTTTCCGTGGCCACCACGAGCAGCAACAAGCAGAAGACAGCGATAAG
AACTCCTCCCAGCAGGCGCAAAATGGCATGTTGCTGCGTGACAACATCTTTGGCTCCATCGAAGATGATGCTCAACGCCG
TGATTTCACCATTAACAGCCTCTACTACGGTATTTCTGATTTTGCGCTGCGTGACTACACCGGCGGCTTACGTGATCTGA
AAGAGGGTATTATTCGTCTAATTGGCGACCCAGAAACCCGTTACCGTGAAGATCCGGTACGGATGCTGCGTGCGGTTCGT
TTTGCCGCAAAACTGGATATGTCAATCAGCCCGGAAACGGCAGAGCCTATACCGCGTCTGGCCTCACTGTTGCGCGAAAT
CCCGCCCGCCCGCCTGTTTGAGGAGTCACTCAAGCTGCTGCAATCCGGCTACGGCTATAAAACCTATTTAAAACTGTGCG
AATACCAGCTTTTCCAGCCGCTATTCCCACTGATTGCCCGTAATTTTACTGAACAGCATGATTCGCCAATGGAGCGTATT
CTGGTTCAGGTACTGAAAAATACCGACCACCGTCTGCATAATGATCAGCGGGTTAATCCTGCCTTCTTATTTGCGGCGAT
GCTGTGGTATCCGCTGATTGAACATGCGCAGAAGTTAACGCAGGAAAGCGGTCTGGCCTATTACGATGCATTTGCATTGG
CCATGAATGATGTGCTGGACGAAGAGTGCCGCTCACTGGCTATCCCTAAACGTATCACCTCACTGGTACGGGATATCTGG
TTGTTACAACTGCGTCTGTCACGTCGTCAGGGGAAACGTGCCCATAAGCTGATGGAACACCCTAAATTCCGGGCGGCTTA
CGATCTGTTACTCCTACGGGCAGAAGTGGAAAAAAATCACGAGCTGCAACGTTTGGCGCAGTGGTGGGGCGAATTCCAGG
AAGCCACTCCCACGCAACAGAAATCCATGCTGAATACATTAGGTGCTGATCCTGCGCCACGGCGTTCACGTCCACGCCGC
CCACGCAAGCCAGTACCCCGGGTATAA

Upstream 100 bases:

>100_bases
GGTGAGCTATGATTAGCCGCTGTTTTTCTGTCGTTCTGTTATTTATTAGCCACTATCGAGGTGTACCATTTTTACCCGAG
TAGCCAATTTCTGCCGTAAG

Downstream 100 bases:

>100_bases
TTTTACCATGATCCGGGTCTATATCGCGCTGGGTAGCAATTTGGCCATGCCACTACAACAGGTTAGCGCGGCACGGGAAG
CCTTGGCGCATCTGCCGCGT

Product: poly(A) polymerase I

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MLIREDKTVRDDKARKDKVPGEDNVARKERRPARAHTGRKGHAVSSSEQRQMAIIPRDQHNISRRDISDNALKVLYRLNK
SGYEAYLVGGGVRDLLLGRKPKDFDITTSATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQQAEDSDK
NSSQQAQNGMLLRDNIFGSIEDDAQRRDFTINSLYYGISDFALRDYTGGLRDLKEGIIRLIGDPETRYREDPVRMLRAVR
FAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLKLCEYQLFQPLFPLIARNFTEQHDSPMERI
LVQVLKNTDHRLHNDQRVNPAFLFAAMLWYPLIEHAQKLTQESGLAYYDAFALAMNDVLDEECRSLAIPKRITSLVRDIW
LLQLRLSRRQGKRAHKLMEHPKFRAAYDLLLLRAEVEKNHELQRLAQWWGEFQEATPTQQKSMLNTLGADPAPRRSRPRR
PRKPVPRV

Sequences:

>Translated_488_residues
MLIREDKTVRDDKARKDKVPGEDNVARKERRPARAHTGRKGHAVSSSEQRQMAIIPRDQHNISRRDISDNALKVLYRLNK
SGYEAYLVGGGVRDLLLGRKPKDFDITTSATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQQAEDSDK
NSSQQAQNGMLLRDNIFGSIEDDAQRRDFTINSLYYGISDFALRDYTGGLRDLKEGIIRLIGDPETRYREDPVRMLRAVR
FAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLKLCEYQLFQPLFPLIARNFTEQHDSPMERI
LVQVLKNTDHRLHNDQRVNPAFLFAAMLWYPLIEHAQKLTQESGLAYYDAFALAMNDVLDEECRSLAIPKRITSLVRDIW
LLQLRLSRRQGKRAHKLMEHPKFRAAYDLLLLRAEVEKNHELQRLAQWWGEFQEATPTQQKSMLNTLGADPAPRRSRPRR
PRKPVPRV
>Mature_488_residues
MLIREDKTVRDDKARKDKVPGEDNVARKERRPARAHTGRKGHAVSSSEQRQMAIIPRDQHNISRRDISDNALKVLYRLNK
SGYEAYLVGGGVRDLLLGRKPKDFDITTSATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQQAEDSDK
NSSQQAQNGMLLRDNIFGSIEDDAQRRDFTINSLYYGISDFALRDYTGGLRDLKEGIIRLIGDPETRYREDPVRMLRAVR
FAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLKLCEYQLFQPLFPLIARNFTEQHDSPMERI
LVQVLKNTDHRLHNDQRVNPAFLFAAMLWYPLIEHAQKLTQESGLAYYDAFALAMNDVLDEECRSLAIPKRITSLVRDIW
LLQLRLSRRQGKRAHKLMEHPKFRAAYDLLLLRAEVEKNHELQRLAQWWGEFQEATPTQQKSMLNTLGADPAPRRSRPRR
PRKPVPRV

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=206, Percent_Identity=32.5242718446602, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI87081691, Length=417, Percent_Identity=80.0959232613909, Blast_Score=710, Evalue=0.0,
Organism=Escherichia coli, GI1789436, Length=230, Percent_Identity=30.8695652173913, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321016, Length=191, Percent_Identity=28.2722513089005, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281360112, Length=276, Percent_Identity=27.8985507246377, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357337, Length=276, Percent_Identity=27.8985507246377, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 56611; Mature: 56611

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIREDKTVRDDKARKDKVPGEDNVARKERRPARAHTGRKGHAVSSSEQRQMAIIPRDQH
CCCCCCCCCCHHHHHHCCCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCCCEEEECCCCCC
NISRRDISDNALKVLYRLNKSGYEAYLVGGGVRDLLLGRKPKDFDITTSATPEQVRKLFR
CCCHHCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
NCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQQAEDSDKNSSQQAQNGMLLRDNIFGSI
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCEEEECCCCCCC
EDDAQRRDFTINSLYYGISDFALRDYTGGLRDLKEGIIRLIGDPETRYREDPVRMLRAVR
CCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHH
FAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLKLCEYQLFQP
HHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LFPLIARNFTEQHDSPMERILVQVLKNTDHRLHNDQRVNPAFLFAAMLWYPLIEHAQKLT
HHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QESGLAYYDAFALAMNDVLDEECRSLAIPKRITSLVRDIWLLQLRLSRRQGKRAHKLMEH
HHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PKFRAAYDLLLLRAEVEKNHELQRLAQWWGEFQEATPTQQKSMLNTLGADPAPRRSRPRR
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCC
PRKPVPRV
CCCCCCCC
>Mature Secondary Structure
MLIREDKTVRDDKARKDKVPGEDNVARKERRPARAHTGRKGHAVSSSEQRQMAIIPRDQH
CCCCCCCCCCHHHHHHCCCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCCCEEEECCCCCC
NISRRDISDNALKVLYRLNKSGYEAYLVGGGVRDLLLGRKPKDFDITTSATPEQVRKLFR
CCCHHCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
NCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQQAEDSDKNSSQQAQNGMLLRDNIFGSI
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCEEEECCCCCCC
EDDAQRRDFTINSLYYGISDFALRDYTGGLRDLKEGIIRLIGDPETRYREDPVRMLRAVR
CCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHH
FAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLKLCEYQLFQP
HHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LFPLIARNFTEQHDSPMERILVQVLKNTDHRLHNDQRVNPAFLFAAMLWYPLIEHAQKLT
HHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QESGLAYYDAFALAMNDVLDEECRSLAIPKRITSLVRDIWLLQLRLSRRQGKRAHKLMEH
HHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PKFRAAYDLLLLRAEVEKNHELQRLAQWWGEFQEATPTQQKSMLNTLGADPAPRRSRPRR
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCC
PRKPVPRV
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]