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The map label for this gene is nadE

Identifier: 50843699

GI number: 50843699

Start: 2450920

End: 2452989

Strand: Direct

Name: nadE

Synonym: PPA2266

Alternate gene names: 50843699

Gene position: 2450920-2452989 (Clockwise)

Preceding gene: 50843695

Following gene: 50843700

Centisome position: 95.73

GC content: 62.17

Gene sequence:

>2070_bases
ATGAAGGGGAACTTCCACTCGACCTATGACCAAGGATTTGCGCGAGTCGCCGCCTGCACCACGACGGTGCATATCGGTGA
CCCCGCCTCGAACGTGGCGGGGATTCTCGACACCTGTCGGGAACTCAGCTCCCGAAGCGTCGCGATCGCCGTGTTCCCCG
AGTTATGTCTGACGGGCTACGCCATCGACGACCTGCTGCTTCAAGACGTCGTGCTTGACGCCGTACTCGACGCCATCGAG
ACTGTGCGCCAAGCTTCCCGTAGCCTCTTCCCGGTCATCATCGTCGGGGCGCCGTTGCAGCGTGGCAACCGGCTTTACAA
CTGTGCGGTCGTGATCCACCGCGGGCAAGTCCTCGGTGTGGTGCCAAAGAGTCACCTCCCCAACTATCGGGAGTTCTACG
AAAAACGTCATTTCGCCGTCGGCGCGGGCACTACCGGGATGATTGACCTATCGGGGCGACAAAAATCTCACGCGGCTTCT
ACTGAGATCCCCTTCGGACCCGACCAGCTCTTCCGAGCCACCGATCTGCCTGACCTCACCTTCCATGTAGAGGTGTGCGA
GGACCTCTGGGTGCCAGTCGCCCCGTCCTCCCAGGCCGCTCTGGCCGGGGCCGTCGTGGAGGTAAATCTCTCGGGCTCCC
CCATCACCGTCGGAAAATCCCGCCAGCGCCACGAGCTGTGCAAAGTCACGTCTTCGCGCAACCTGCAGGCCTACGTCTAT
GCGGCCGCCGGTCCCGGAGAATCCAGCACAGATCTGTCCTGGGACGGCCAAACAATGATCTACGAAAACGGTTCCCTCCT
TGCCGCCACCGACAGATTCTCCCCCGAACCGGGCTACTGCCTCGCCGATATCGACCTCGACCTGCTGCGGCAGGAGCGGT
TGCGTCAGGGGTCCTTCGACGACAACGCCCTCGCCCAGCCCGCTCAAGCTCCATGGCGCACCACCATCTTCACTCTTGAC
CCGCCCCACGACGACATCGGCCTGGAACGCCCCGTTAATCGCTTCCCCTTCGTCCCCAACGACCCTGACCAGCTCGCCCA
AGATTGCTACGAGGCCTACAACATCCAGGTCTACGGACTGCGTCGTCGACTCGAGTCAATGGGATCACCGAAAATCGTCA
TTGGGGTTTCCGGCGGTCTCGACTCCACCCACGCCCTGCTCGTCGCCGCCAAAGCCATGGACCAGATGGGCCGGCCGCGC
ACCGACATCCTTGCTTTCACAATGCCGGGGTTCGCTACCACCGACCACACCAAAAACAACGCCCTAGATATGTGTCGCGC
CCTCGGCATTCCGTGCGAAGTGTTGGACATCCGGCCGGCTGCCACCCAGATGCTCAAGGGAATGAGCCATCCCGCCGGTG
ACGGCGCCGAAGTATACGACGTCACCTTCGAGAACGTCCAGGCGGGGCTGCGCTACGACTACCTCTTCCGGATAGCCAAC
CAGCGCGGCGGCATCGTGCTGGGCACCGGTGATTTGTCGGAACTAGCACTGGGCTGGTGCACCTTCGGGGTCGGCGATCA
GATGAGCCACTACGGGGTCAATACCGGGGTACCCAAGACCCTCATTCAGCACCTCATTCGGTGGTGCATTTCGTCGGGCC
AGTTCAACGAGGCCACTAATGTGGTGCTCGAGTCGGTGCTGGGAACAGAAATCTCCCCCGAGCTCGTCCCCGCTCGTCCC
GGCGAGAAGATCCAGTCCACTCAAGCGAAGATAGGTCCCTACGAGCTGCAGGACTTCACCCTGTACCACCTGCTGCGCCA
CGGGATGCGCCCCAGCCGGATCATTTTCCTGTCCCACCATGCCTGGCGGGATGCATCGGTAGGCTCCTGGCCTCCCGGGT
TCCGTGACGAGGACCGACACTCCTATGACCTGTCAGCAATCAAGGACTGGACGCGGCTGTTTCTGCGCCGGTTCGTCGGA
AATCAGTTCAAGAGGTCGACTCTTCCCAACGGTCCCAAGGTCGTCGCAGGAGGTTCACTGAGTCCGCGCGGTGACTGGCG
GATGCCCTCGGATGCCGTCGTCCGGGCCTGGCTTGACGACCTTGAGACAGTTCCCGACGAGCGGTTCTAA

Upstream 100 bases:

>100_bases
GATTACCGTCTCCCGCACCGGCGCGAACCCACCGCGCACCGCCGAATTAGGGTGACCGAAGATTCCTCACCCCCGCGTCA
ATGTCTGCGAGACTGGCATC

Downstream 100 bases:

>100_bases
ACCGTCACGGTCTGTTCGTTACGGTCTGCGCCCGGGGGATAATCCTCGCCCCGAGCCCCAGTTTTGGGCGCCTTCTGCGA
CCGGTGACGAAAAATCCCAG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 689; Mature: 689

Protein sequence:

>689_residues
MKGNFHSTYDQGFARVAACTTTVHIGDPASNVAGILDTCRELSSRSVAIAVFPELCLTGYAIDDLLLQDVVLDAVLDAIE
TVRQASRSLFPVIIVGAPLQRGNRLYNCAVVIHRGQVLGVVPKSHLPNYREFYEKRHFAVGAGTTGMIDLSGRQKSHAAS
TEIPFGPDQLFRATDLPDLTFHVEVCEDLWVPVAPSSQAALAGAVVEVNLSGSPITVGKSRQRHELCKVTSSRNLQAYVY
AAAGPGESSTDLSWDGQTMIYENGSLLAATDRFSPEPGYCLADIDLDLLRQERLRQGSFDDNALAQPAQAPWRTTIFTLD
PPHDDIGLERPVNRFPFVPNDPDQLAQDCYEAYNIQVYGLRRRLESMGSPKIVIGVSGGLDSTHALLVAAKAMDQMGRPR
TDILAFTMPGFATTDHTKNNALDMCRALGIPCEVLDIRPAATQMLKGMSHPAGDGAEVYDVTFENVQAGLRYDYLFRIAN
QRGGIVLGTGDLSELALGWCTFGVGDQMSHYGVNTGVPKTLIQHLIRWCISSGQFNEATNVVLESVLGTEISPELVPARP
GEKIQSTQAKIGPYELQDFTLYHLLRHGMRPSRIIFLSHHAWRDASVGSWPPGFRDEDRHSYDLSAIKDWTRLFLRRFVG
NQFKRSTLPNGPKVVAGGSLSPRGDWRMPSDAVVRAWLDDLETVPDERF

Sequences:

>Translated_689_residues
MKGNFHSTYDQGFARVAACTTTVHIGDPASNVAGILDTCRELSSRSVAIAVFPELCLTGYAIDDLLLQDVVLDAVLDAIE
TVRQASRSLFPVIIVGAPLQRGNRLYNCAVVIHRGQVLGVVPKSHLPNYREFYEKRHFAVGAGTTGMIDLSGRQKSHAAS
TEIPFGPDQLFRATDLPDLTFHVEVCEDLWVPVAPSSQAALAGAVVEVNLSGSPITVGKSRQRHELCKVTSSRNLQAYVY
AAAGPGESSTDLSWDGQTMIYENGSLLAATDRFSPEPGYCLADIDLDLLRQERLRQGSFDDNALAQPAQAPWRTTIFTLD
PPHDDIGLERPVNRFPFVPNDPDQLAQDCYEAYNIQVYGLRRRLESMGSPKIVIGVSGGLDSTHALLVAAKAMDQMGRPR
TDILAFTMPGFATTDHTKNNALDMCRALGIPCEVLDIRPAATQMLKGMSHPAGDGAEVYDVTFENVQAGLRYDYLFRIAN
QRGGIVLGTGDLSELALGWCTFGVGDQMSHYGVNTGVPKTLIQHLIRWCISSGQFNEATNVVLESVLGTEISPELVPARP
GEKIQSTQAKIGPYELQDFTLYHLLRHGMRPSRIIFLSHHAWRDASVGSWPPGFRDEDRHSYDLSAIKDWTRLFLRRFVG
NQFKRSTLPNGPKVVAGGSLSPRGDWRMPSDAVVRAWLDDLETVPDERF
>Mature_689_residues
MKGNFHSTYDQGFARVAACTTTVHIGDPASNVAGILDTCRELSSRSVAIAVFPELCLTGYAIDDLLLQDVVLDAVLDAIE
TVRQASRSLFPVIIVGAPLQRGNRLYNCAVVIHRGQVLGVVPKSHLPNYREFYEKRHFAVGAGTTGMIDLSGRQKSHAAS
TEIPFGPDQLFRATDLPDLTFHVEVCEDLWVPVAPSSQAALAGAVVEVNLSGSPITVGKSRQRHELCKVTSSRNLQAYVY
AAAGPGESSTDLSWDGQTMIYENGSLLAATDRFSPEPGYCLADIDLDLLRQERLRQGSFDDNALAQPAQAPWRTTIFTLD
PPHDDIGLERPVNRFPFVPNDPDQLAQDCYEAYNIQVYGLRRRLESMGSPKIVIGVSGGLDSTHALLVAAKAMDQMGRPR
TDILAFTMPGFATTDHTKNNALDMCRALGIPCEVLDIRPAATQMLKGMSHPAGDGAEVYDVTFENVQAGLRYDYLFRIAN
QRGGIVLGTGDLSELALGWCTFGVGDQMSHYGVNTGVPKTLIQHLIRWCISSGQFNEATNVVLESVLGTEISPELVPARP
GEKIQSTQAKIGPYELQDFTLYHLLRHGMRPSRIIFLSHHAWRDASVGSWPPGFRDEDRHSYDLSAIKDWTRLFLRRFVG
NQFKRSTLPNGPKVVAGGSLSPRGDWRMPSDAVVRAWLDDLETVPDERF

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=687, Percent_Identity=22.5618631732169, Blast_Score=106, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17538756, Length=688, Percent_Identity=23.1104651162791, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6321865, Length=652, Percent_Identity=23.0061349693252, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24641841, Length=610, Percent_Identity=22.9508196721311, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24641843, Length=610, Percent_Identity=22.9508196721311, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 75692; Mature: 75692

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00589 PTS_HPR_SER ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGNFHSTYDQGFARVAACTTTVHIGDPASNVAGILDTCRELSSRSVAIAVFPELCLTGY
CCCCCCCHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHCH
AIDDLLLQDVVLDAVLDAIETVRQASRSLFPVIIVGAPLQRGNRLYNCAVVIHRGQVLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHCCEEEEEEEEEECCCEEEE
VPKSHLPNYREFYEKRHFAVGAGTTGMIDLSGRQKSHAASTEIPFGPDQLFRATDLPDLT
CCHHHCCCHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCE
FHVEVCEDLWVPVAPSSQAALAGAVVEVNLSGSPITVGKSRQRHELCKVTSSRNLQAYVY
EEEEEHHHCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCHHHHHHHHHHCCCCEEEEEE
AAAGPGESSTDLSWDGQTMIYENGSLLAATDRFSPEPGYCLADIDLDLLRQERLRQGSFD
EECCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCEEECCCHHHHHHHHHHCCCCC
DNALAQPAQAPWRTTIFTLDPPHDDIGLERPVNRFPFVPNDPDQLAQDCYEAYNIQVYGL
CCHHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCEEEEEH
RRRLESMGSPKIVIGVSGGLDSTHALLVAAKAMDQMGRPRTDILAFTMPGFATTDHTKNN
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCH
ALDMCRALGIPCEVLDIRPAATQMLKGMSHPAGDGAEVYDVTFENVQAGLRYDYLFRIAN
HHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCEEEEEEHHHHHCCCCCEEHHEECC
QRGGIVLGTGDLSELALGWCTFGVGDQMSHYGVNTGVPKTLIQHLIRWCISSGQFNEATN
CCCCEEEECCCHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
VVLESVLGTEISPELVPARPGEKIQSTQAKIGPYELQDFTLYHLLRHGMRPSRIIFLSHH
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEECHHHHHHHHHHCCCCCCEEEEEECC
AWRDASVGSWPPGFRDEDRHSYDLSAIKDWTRLFLRRFVGNQFKRSTLPNGPKVVAGGSL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEECCCC
SPRGDWRMPSDAVVRAWLDDLETVPDERF
CCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKGNFHSTYDQGFARVAACTTTVHIGDPASNVAGILDTCRELSSRSVAIAVFPELCLTGY
CCCCCCCHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHCH
AIDDLLLQDVVLDAVLDAIETVRQASRSLFPVIIVGAPLQRGNRLYNCAVVIHRGQVLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHCCEEEEEEEEEECCCEEEE
VPKSHLPNYREFYEKRHFAVGAGTTGMIDLSGRQKSHAASTEIPFGPDQLFRATDLPDLT
CCHHHCCCHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCE
FHVEVCEDLWVPVAPSSQAALAGAVVEVNLSGSPITVGKSRQRHELCKVTSSRNLQAYVY
EEEEEHHHCCCCCCCCCCCEEEEEEEEEECCCCCEEECCCHHHHHHHHHHCCCCEEEEEE
AAAGPGESSTDLSWDGQTMIYENGSLLAATDRFSPEPGYCLADIDLDLLRQERLRQGSFD
EECCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCEEECCCHHHHHHHHHHCCCCC
DNALAQPAQAPWRTTIFTLDPPHDDIGLERPVNRFPFVPNDPDQLAQDCYEAYNIQVYGL
CCHHCCCCCCCCEEEEEEECCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCEEEEEH
RRRLESMGSPKIVIGVSGGLDSTHALLVAAKAMDQMGRPRTDILAFTMPGFATTDHTKNN
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCH
ALDMCRALGIPCEVLDIRPAATQMLKGMSHPAGDGAEVYDVTFENVQAGLRYDYLFRIAN
HHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCEEEEEEHHHHHCCCCCEEHHEECC
QRGGIVLGTGDLSELALGWCTFGVGDQMSHYGVNTGVPKTLIQHLIRWCISSGQFNEATN
CCCCEEEECCCHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
VVLESVLGTEISPELVPARPGEKIQSTQAKIGPYELQDFTLYHLLRHGMRPSRIIFLSHH
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEECHHHHHHHHHHCCCCCCEEEEEECC
AWRDASVGSWPPGFRDEDRHSYDLSAIKDWTRLFLRRFVGNQFKRSTLPNGPKVVAGGSL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEECCCC
SPRGDWRMPSDAVVRAWLDDLETVPDERF
CCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]