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The map label for this gene is pfkA [H]

Identifier: 50843700

GI number: 50843700

Start: 2453168

End: 2455414

Strand: Direct

Name: pfkA [H]

Synonym: PPA2267

Alternate gene names: 50843700

Gene position: 2453168-2455414 (Clockwise)

Preceding gene: 50843699

Following gene: 50843702

Centisome position: 95.82

GC content: 62.44

Gene sequence:

>2247_bases
ATGAGGCTCACCCCCACTCTCCCCGACGGTCATGGTCTGCGCATTGGCATCCTGACCAGCGGTGGCGACGCTCAGGGCAT
GAACGCTGCTGTGCGGGCTGTGGTGCGCTCTGCGCTAAGTGTGGGAGCCGAGGTCTATGCGATATACGAGGGCTACCAGG
GAATGATCGATGGTGGCGATGGCATCCGTAGGTTCGGGTGGGACGACGTCGGCTCGATCCTCAACCGTGGCGGCACCGTG
ATCGGAACCTTCCGTTGCAAGGGGATGCGCGAACGTCCGGGGCGCCTGAAGGCTGTCAGAAATCTCCTCGAACACGGGAT
CGATCGTCTCGTCGTCATCGGCGGTGACGGTTCGCTCACCGGCCTGGATGTGCTGCGCACCGAATGGACAGACCTGGTCG
CTGAACTCGTCGACACCGGCCAGGTGAGCGCCGAGGTAGCCCGGGAACACCCCGCCCTCATGATCGCCGGGCTCGTCGGA
TCCATCGACAATGACCTCGTCGGATCCGATATGACCATCGGTGCCGATACTGCTCTGCACCGTATTGTCGATGCCATCGA
CGACCTCGCCTCCACCGCGGCCAGCCACCAGCGATCCTTCGTCGTTGAGGTGATGGGCCGCCACTGCGGCTATCTCGCCC
TTATGGCAGCAGTTGCCGGCGGAGCTGACTATGTTCTTATTCCGGAACGGCCGCCCGAGGACGGTTGGGAGGACCGCATG
TGCGCCGAGCTCAAACGTGGCCGTCAGGCGGGCCGACGCGACTCGATCGTCGTCGTCGCGGAGGGCGCCACCGATCGTTC
CGGGAACCGGATCTCCTCGGAGTACGTCCGCCAGGTGCTTGAGGACAAACTCGGTGAGGACGCCCGGGTTACCATCCTGG
GCCACGTCCAGCGCGGCGGACGACCGTCAGCCTATGACCGCTGGGCGTCGACCTGGTTGGGTTATCACGCGGTTCACGAG
GTGCTCTCGACCACTCCAGAGGCCGAGGGGCGCGTTATCGGCACTGGGTCCAACCGCATACATCGCCTCCCCCTGGTCAA
GGCCGTCGCTGACACGAAGTCGGTGCCCGGTCTCATTACGGAGGGCCGTTACAACGAGGCCATGCGGATGAGGGGACGTT
CCTTTGTCGAGATGGACGCGATCTTCACCGAACTCAGTGAACCAGCTCGTACCACTTCCGACACCGCTGGTGACGCCGAC
CGCAAGCGCGTCGCGATCATGCACGCTGGCGGTCCGGCCCCGGGCATGAATACTGCTGCCCGTGCTGCAGTGCGACTGGC
GATTTCCCGCGGTCATACCGTTGTGGGAATCCATAACGGGTTCATCGGACTAGCGACCGGGGACATGTCGGAATTGGCCT
GGAGCGACGTCGAGGGCTGGGCCGGGGAAGGCGGCGCTGAGCTGGGTACCCGCCGCGAGATCCCTAGCACTCGCGACCTT
TATGCTGTCAGCCGCGCTCTGGAGGATCATCACATCGACGCGTTGCTCGTCATTGGTGGCTACTCGGCCTACGAAGGTAT
CTACACCATGATGACTGAGCGGGACCGGTACCCGGCTTTCCGTATTCCGACGGTGTGCATTCCGGCTTCGATCGACAACA
ACCTCCCCGGTTCGGAACTGTCCATCGGCACCGACACCGCTCTCAACGTCATTGTCGAGGCGATGGACAAGATTAAAGAG
TCGGGTATCGCGTCCAGACGCTGCTTCGTCGTCGAGACGATGGGTCGTGACTGCGGATACCTCGCGCTGATGTCGGGTAT
CGCAGCTGGCGCTGAGCGGATCTATACCAACGAGGACGGCATCTCCCTGGACGATCTAGCCAACGACGTCCATTGGTTGC
GGGAGTCATTCGCCCACGGACGACGACTTTTCCTTGCCGTGCGCAACGAGAACGCATCGCACAATTACACCACCGACTTC
ATCGCGCGGCTGCTGGAGGAGGAATCACACGGCATGTATGACGTACGCCAGGTGGTACTAGGGCATATGCAGCAAGGCGG
CTCACCCTCACCTTTCGACCGCTTGCTCGCCAACCGACTCGGGTATCGCGCTCTCAATCTCATTGACGACGAGTTGGCGG
CGCATCAGGACGGTTCATGGTTCATTGGCGTCAACGAGTCGGGCATGCGGCCCTGTTCGATGGACACCATGCCCTCTCTC
ATTGACGCCGCTCATCGTCGTCCCCGCGAACAGTGGTGGCTGCAACTGAGGACTATTGCCAGGATGGTCTCGGACGAGGT
GCGGTAA

Upstream 100 bases:

>100_bases
GACCGGTGACGAAAAATCCCAGCAGCCAGGACGAGCAGTCACAATTCACCTGATACCCACCCGCGCGCGGCAATATTGAT
CTGTGCATGTTGATGACGAG

Downstream 100 bases:

>100_bases
GTCGCGACGACCTCTCGCGAACTCTAGGTAGGTGGGGGTGGTGTGGTTTCACCCCCACCACCAACCTCACAACACCTCAG
ACACAGGCACACACTCAATA

Product: 6-phosphofructokinase, type C

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MRLTPTLPDGHGLRIGILTSGGDAQGMNAAVRAVVRSALSVGAEVYAIYEGYQGMIDGGDGIRRFGWDDVGSILNRGGTV
IGTFRCKGMRERPGRLKAVRNLLEHGIDRLVVIGGDGSLTGLDVLRTEWTDLVAELVDTGQVSAEVAREHPALMIAGLVG
SIDNDLVGSDMTIGADTALHRIVDAIDDLASTAASHQRSFVVEVMGRHCGYLALMAAVAGGADYVLIPERPPEDGWEDRM
CAELKRGRQAGRRDSIVVVAEGATDRSGNRISSEYVRQVLEDKLGEDARVTILGHVQRGGRPSAYDRWASTWLGYHAVHE
VLSTTPEAEGRVIGTGSNRIHRLPLVKAVADTKSVPGLITEGRYNEAMRMRGRSFVEMDAIFTELSEPARTTSDTAGDAD
RKRVAIMHAGGPAPGMNTAARAAVRLAISRGHTVVGIHNGFIGLATGDMSELAWSDVEGWAGEGGAELGTRREIPSTRDL
YAVSRALEDHHIDALLVIGGYSAYEGIYTMMTERDRYPAFRIPTVCIPASIDNNLPGSELSIGTDTALNVIVEAMDKIKE
SGIASRRCFVVETMGRDCGYLALMSGIAAGAERIYTNEDGISLDDLANDVHWLRESFAHGRRLFLAVRNENASHNYTTDF
IARLLEEESHGMYDVRQVVLGHMQQGGSPSPFDRLLANRLGYRALNLIDDELAAHQDGSWFIGVNESGMRPCSMDTMPSL
IDAAHRRPREQWWLQLRTIARMVSDEVR

Sequences:

>Translated_748_residues
MRLTPTLPDGHGLRIGILTSGGDAQGMNAAVRAVVRSALSVGAEVYAIYEGYQGMIDGGDGIRRFGWDDVGSILNRGGTV
IGTFRCKGMRERPGRLKAVRNLLEHGIDRLVVIGGDGSLTGLDVLRTEWTDLVAELVDTGQVSAEVAREHPALMIAGLVG
SIDNDLVGSDMTIGADTALHRIVDAIDDLASTAASHQRSFVVEVMGRHCGYLALMAAVAGGADYVLIPERPPEDGWEDRM
CAELKRGRQAGRRDSIVVVAEGATDRSGNRISSEYVRQVLEDKLGEDARVTILGHVQRGGRPSAYDRWASTWLGYHAVHE
VLSTTPEAEGRVIGTGSNRIHRLPLVKAVADTKSVPGLITEGRYNEAMRMRGRSFVEMDAIFTELSEPARTTSDTAGDAD
RKRVAIMHAGGPAPGMNTAARAAVRLAISRGHTVVGIHNGFIGLATGDMSELAWSDVEGWAGEGGAELGTRREIPSTRDL
YAVSRALEDHHIDALLVIGGYSAYEGIYTMMTERDRYPAFRIPTVCIPASIDNNLPGSELSIGTDTALNVIVEAMDKIKE
SGIASRRCFVVETMGRDCGYLALMSGIAAGAERIYTNEDGISLDDLANDVHWLRESFAHGRRLFLAVRNENASHNYTTDF
IARLLEEESHGMYDVRQVVLGHMQQGGSPSPFDRLLANRLGYRALNLIDDELAAHQDGSWFIGVNESGMRPCSMDTMPSL
IDAAHRRPREQWWLQLRTIARMVSDEVR
>Mature_748_residues
MRLTPTLPDGHGLRIGILTSGGDAQGMNAAVRAVVRSALSVGAEVYAIYEGYQGMIDGGDGIRRFGWDDVGSILNRGGTV
IGTFRCKGMRERPGRLKAVRNLLEHGIDRLVVIGGDGSLTGLDVLRTEWTDLVAELVDTGQVSAEVAREHPALMIAGLVG
SIDNDLVGSDMTIGADTALHRIVDAIDDLASTAASHQRSFVVEVMGRHCGYLALMAAVAGGADYVLIPERPPEDGWEDRM
CAELKRGRQAGRRDSIVVVAEGATDRSGNRISSEYVRQVLEDKLGEDARVTILGHVQRGGRPSAYDRWASTWLGYHAVHE
VLSTTPEAEGRVIGTGSNRIHRLPLVKAVADTKSVPGLITEGRYNEAMRMRGRSFVEMDAIFTELSEPARTTSDTAGDAD
RKRVAIMHAGGPAPGMNTAARAAVRLAISRGHTVVGIHNGFIGLATGDMSELAWSDVEGWAGEGGAELGTRREIPSTRDL
YAVSRALEDHHIDALLVIGGYSAYEGIYTMMTERDRYPAFRIPTVCIPASIDNNLPGSELSIGTDTALNVIVEAMDKIKE
SGIASRRCFVVETMGRDCGYLALMSGIAAGAERIYTNEDGISLDDLANDVHWLRESFAHGRRLFLAVRNENASHNYTTDF
IARLLEEESHGMYDVRQVVLGHMQQGGSPSPFDRLLANRLGYRALNLIDDELAAHQDGSWFIGVNESGMRPCSMDTMPSL
IDAAHRRPREQWWLQLRTIARMVSDEVR

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=749, Percent_Identity=46.8624833110814, Blast_Score=690, Evalue=0.0,
Organism=Homo sapiens, GI11321601, Length=745, Percent_Identity=46.8456375838926, Blast_Score=687, Evalue=0.0,
Organism=Homo sapiens, GI266453619, Length=746, Percent_Identity=46.2466487935657, Blast_Score=676, Evalue=0.0,
Organism=Homo sapiens, GI266453768, Length=746, Percent_Identity=46.2466487935657, Blast_Score=675, Evalue=0.0,
Organism=Homo sapiens, GI266453748, Length=746, Percent_Identity=46.2466487935657, Blast_Score=675, Evalue=0.0,
Organism=Homo sapiens, GI4505749, Length=746, Percent_Identity=46.2466487935657, Blast_Score=675, Evalue=0.0,
Organism=Escherichia coli, GI1790350, Length=340, Percent_Identity=40.2941176470588, Blast_Score=213, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI25147584, Length=746, Percent_Identity=44.5040214477212, Blast_Score=663, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17558788, Length=754, Percent_Identity=39.1246684350133, Blast_Score=521, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI72003601, Length=574, Percent_Identity=41.6376306620209, Blast_Score=472, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6321679, Length=684, Percent_Identity=44.7368421052632, Blast_Score=566, Evalue=1e-162,
Organism=Saccharomyces cerevisiae, GI6323861, Length=684, Percent_Identity=43.2748538011696, Blast_Score=562, Evalue=1e-161,
Organism=Drosophila melanogaster, GI17647809, Length=734, Percent_Identity=46.3215258855586, Blast_Score=671, Evalue=0.0,
Organism=Drosophila melanogaster, GI24652337, Length=734, Percent_Identity=46.3215258855586, Blast_Score=671, Evalue=0.0,
Organism=Drosophila melanogaster, GI28573326, Length=734, Percent_Identity=46.1852861035422, Blast_Score=667, Evalue=0.0,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 81295; Mature: 81295

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTPTLPDGHGLRIGILTSGGDAQGMNAAVRAVVRSALSVGAEVYAIYEGYQGMIDGGD
CCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEEEEEECCCCEECCCC
GIRRFGWDDVGSILNRGGTVIGTFRCKGMRERPGRLKAVRNLLEHGIDRLVVIGGDGSLT
CHHHCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
GLDVLRTEWTDLVAELVDTGQVSAEVAREHPALMIAGLVGSIDNDLVGSDMTIGADTALH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEHHHHCCCCCCCCCCCEECHHHHHH
RIVDAIDDLASTAASHQRSFVVEVMGRHCGYLALMAAVAGGADYVLIPERPPEDGWEDRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHH
CAELKRGRQAGRRDSIVVVAEGATDRSGNRISSEYVRQVLEDKLGEDARVTILGHVQRGG
HHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
RPSAYDRWASTWLGYHAVHEVLSTTPEAEGRVIGTGSNRIHRLPLVKAVADTKSVPGLIT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEECHHHHHHHHCCCCCCCCC
EGRYNEAMRMRGRSFVEMDAIFTELSEPARTTSDTAGDADRKRVAIMHAGGPAPGMNTAA
CCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHH
RAAVRLAISRGHTVVGIHNGFIGLATGDMSELAWSDVEGWAGEGGAELGTRREIPSTRDL
HHHHHHHHHCCCEEEEEECCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHH
YAVSRALEDHHIDALLVIGGYSAYEGIYTMMTERDRYPAFRIPTVCIPASIDNNLPGSEL
HHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCEECCCEEEECCCCCCCCCCCC
SIGTDTALNVIVEAMDKIKESGIASRRCFVVETMGRDCGYLALMSGIAAGAERIYTNEDG
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEECCCC
ISLDDLANDVHWLRESFAHGRRLFLAVRNENASHNYTTDFIARLLEEESHGMYDVRQVVL
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
GHMQQGGSPSPFDRLLANRLGYRALNLIDDELAAHQDGSWFIGVNESGMRPCSMDTMPSL
HHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHH
IDAAHRRPREQWWLQLRTIARMVSDEVR
HHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLTPTLPDGHGLRIGILTSGGDAQGMNAAVRAVVRSALSVGAEVYAIYEGYQGMIDGGD
CCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEEEEEECCCCEECCCC
GIRRFGWDDVGSILNRGGTVIGTFRCKGMRERPGRLKAVRNLLEHGIDRLVVIGGDGSLT
CHHHCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
GLDVLRTEWTDLVAELVDTGQVSAEVAREHPALMIAGLVGSIDNDLVGSDMTIGADTALH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEHHHHCCCCCCCCCCCEECHHHHHH
RIVDAIDDLASTAASHQRSFVVEVMGRHCGYLALMAAVAGGADYVLIPERPPEDGWEDRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHH
CAELKRGRQAGRRDSIVVVAEGATDRSGNRISSEYVRQVLEDKLGEDARVTILGHVQRGG
HHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
RPSAYDRWASTWLGYHAVHEVLSTTPEAEGRVIGTGSNRIHRLPLVKAVADTKSVPGLIT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEECHHHHHHHHCCCCCCCCC
EGRYNEAMRMRGRSFVEMDAIFTELSEPARTTSDTAGDADRKRVAIMHAGGPAPGMNTAA
CCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHH
RAAVRLAISRGHTVVGIHNGFIGLATGDMSELAWSDVEGWAGEGGAELGTRREIPSTRDL
HHHHHHHHHCCCEEEEEECCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHH
YAVSRALEDHHIDALLVIGGYSAYEGIYTMMTERDRYPAFRIPTVCIPASIDNNLPGSEL
HHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCEECCCEEEECCCCCCCCCCCC
SIGTDTALNVIVEAMDKIKESGIASRRCFVVETMGRDCGYLALMSGIAAGAERIYTNEDG
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHEEECCCC
ISLDDLANDVHWLRESFAHGRRLFLAVRNENASHNYTTDFIARLLEEESHGMYDVRQVVL
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
GHMQQGGSPSPFDRLLANRLGYRALNLIDDELAAHQDGSWFIGVNESGMRPCSMDTMPSL
HHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHH
IDAAHRRPREQWWLQLRTIARMVSDEVR
HHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA