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The map label for this gene is argG [H]

Identifier: 50843636

GI number: 50843636

Start: 2381756

End: 2383180

Strand: Direct

Name: argG [H]

Synonym: PPA2201

Alternate gene names: 50843636

Gene position: 2381756-2383180 (Clockwise)

Preceding gene: 50843635

Following gene: 50843637

Centisome position: 93.03

GC content: 61.82

Gene sequence:

>1425_bases
GTGGTTGCCGTGTCCAAGGTCATCACTCAGCTTCCCGTCGGCGAGCGCGTCGGCATCGCATTCTCCGGAGGACTCGACAC
CTCCGTCGCTGTCGCGTGGATGCGCGAAAATGGCGCGGTTCCGTGCACCTACACCGCCGACATTGGTCAGTACGACGAGC
CCGACATCGCGTCAGTGCCGGGTCGCGCCCTCGACTACGGCGCTGAGATCTCCCGACTTGTCGACTGCAAGGCAGCGCTC
GTCGAGGAGGGACTGAGCGCTATCGCGTGCGGCGCGTTCAACGTTCGCTCCGCCGGGCGCCCCTACTTCAACACCACCCC
AATCGGTCGCGCCGTCACCGGCACTCTGTTGGTACGCGCTATGCATACCGACGACGTCTCCATTTGGGGCGACGGCTCCA
CCTATAAGGGCAACGACATCGAGCGGTTCTACCGCTACGGCTTACTCGCCAACCCGCAGCTGCGCATCTATAAGCCGTGG
CTCGACGAAAACTTCGTCGCTGAACTCGGTGGCCGTGACGAGATGAGCGCCTGGCTTACCGCCCACAACCTGCCTTATCG
CGACTCCAAGGAAAAGGCCTATTCCACCGACGCCAACATCTGGGGCGCCACCCACGAGGCCAAGAAACTTGAATCCCTGC
ACGTCTCCATCGAGTCCGTCAACCCGATCATGGGCGTCAAGTTCTGGGACCCAGACGTCAAGATCGATACTGAGGAGGTG
ACGGTCTCCTTCGAGCAGGGCCTGCCCGTCGCCATCAATGGCAAGGAATACGACGATAAAGTCGCCCTCGTTCTGGAGGC
CAACGCCATCGGCGGTCGTCACGGCCTCGGTATGAGCGACCAGATCGAAAATCGCATTATTGAGGCGAAATCCCGCGGCA
TCTATGAGGCCCCGGGCATGGCCCTGCTCCACATCGCCTACGAACGTCTCGCCAACGCCATCCACAACGAAGACACTCTG
GAGACTTATCACAACGAGGGGCGTCGCCTCGGGCGGCTGCTCTACGAGGGACGTTGGCTCGACCCGCAGTCCCTCATGAT
CCGTGAATCTCTCACCCACTGGGTCGCCAGCGCCATTACTGGATCGGTCACGCTCAAGCTGCGTCGTGGCTGGGACTACT
CCATCCTTGACACCACCGGACCGAACTTCTCGTACCACTCTGAGAAGTTGTCGATGGAGCGCGTTGAGGGGGCTGCCTTC
GGCCCGACCGATCGCATCGGCCAGCTCACGATGCGCAACCTCGATATCGCCGATACCCGCGAAAAGCTTGAGCTCTACTC
CGCGCAAGGCCAGCTCGCCAGCCACGCCGACCTCGTTGGGCAGCTTGAGACCGGCGGCGCATCAGCTATCGCGTCCAACG
GCGACGATGACGACGACAGCCAAAAGACTCTCGACGCTGCCGCAATGGAAGTTGGCGTCAACTGA

Upstream 100 bases:

>100_bases
TCCCGTCCGCCAGCTGATAGCCCATACCTCATCATCTCCATCAACTTTCAGCTTAGCCAACCGCATATCCATTTGGGACG
CTGCATATATATGCGCTAGA

Downstream 100 bases:

>100_bases
TCGCCACATGTTCGGGCCGCCCGGGATACCTGGTCGGCCCGAATGAGTTTTCTTGAGCCACCTCGCGCCATCAGAAAGGT
GACTCATCACGTAACGCGCT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MVAVSKVITQLPVGERVGIAFSGGLDTSVAVAWMRENGAVPCTYTADIGQYDEPDIASVPGRALDYGAEISRLVDCKAAL
VEEGLSAIACGAFNVRSAGRPYFNTTPIGRAVTGTLLVRAMHTDDVSIWGDGSTYKGNDIERFYRYGLLANPQLRIYKPW
LDENFVAELGGRDEMSAWLTAHNLPYRDSKEKAYSTDANIWGATHEAKKLESLHVSIESVNPIMGVKFWDPDVKIDTEEV
TVSFEQGLPVAINGKEYDDKVALVLEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLANAIHNEDTL
ETYHNEGRRLGRLLYEGRWLDPQSLMIRESLTHWVASAITGSVTLKLRRGWDYSILDTTGPNFSYHSEKLSMERVEGAAF
GPTDRIGQLTMRNLDIADTREKLELYSAQGQLASHADLVGQLETGGASAIASNGDDDDDSQKTLDAAAMEVGVN

Sequences:

>Translated_474_residues
MVAVSKVITQLPVGERVGIAFSGGLDTSVAVAWMRENGAVPCTYTADIGQYDEPDIASVPGRALDYGAEISRLVDCKAAL
VEEGLSAIACGAFNVRSAGRPYFNTTPIGRAVTGTLLVRAMHTDDVSIWGDGSTYKGNDIERFYRYGLLANPQLRIYKPW
LDENFVAELGGRDEMSAWLTAHNLPYRDSKEKAYSTDANIWGATHEAKKLESLHVSIESVNPIMGVKFWDPDVKIDTEEV
TVSFEQGLPVAINGKEYDDKVALVLEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLANAIHNEDTL
ETYHNEGRRLGRLLYEGRWLDPQSLMIRESLTHWVASAITGSVTLKLRRGWDYSILDTTGPNFSYHSEKLSMERVEGAAF
GPTDRIGQLTMRNLDIADTREKLELYSAQGQLASHADLVGQLETGGASAIASNGDDDDDSQKTLDAAAMEVGVN
>Mature_474_residues
MVAVSKVITQLPVGERVGIAFSGGLDTSVAVAWMRENGAVPCTYTADIGQYDEPDIASVPGRALDYGAEISRLVDCKAAL
VEEGLSAIACGAFNVRSAGRPYFNTTPIGRAVTGTLLVRAMHTDDVSIWGDGSTYKGNDIERFYRYGLLANPQLRIYKPW
LDENFVAELGGRDEMSAWLTAHNLPYRDSKEKAYSTDANIWGATHEAKKLESLHVSIESVNPIMGVKFWDPDVKIDTEEV
TVSFEQGLPVAINGKEYDDKVALVLEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLANAIHNEDTL
ETYHNEGRRLGRLLYEGRWLDPQSLMIRESLTHWVASAITGSVTLKLRRGWDYSILDTTGPNFSYHSEKLSMERVEGAAF
GPTDRIGQLTMRNLDIADTREKLELYSAQGQLASHADLVGQLETGGASAIASNGDDDDDSQKTLDAAAMEVGVN

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=411, Percent_Identity=27.9805352798054, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI16950633, Length=411, Percent_Identity=27.9805352798054, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1789563, Length=433, Percent_Identity=66.9745958429561, Blast_Score=606, Evalue=1e-174,
Organism=Saccharomyces cerevisiae, GI6324514, Length=420, Percent_Identity=27.6190476190476, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21358151, Length=424, Percent_Identity=27.122641509434, Blast_Score=145, Evalue=7e-35,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 51863; Mature: 51863

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVSKVITQLPVGERVGIAFSGGLDTSVAVAWMRENGAVPCTYTADIGQYDEPDIASVP
CCCHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCC
GRALDYGAEISRLVDCKAALVEEGLSAIACGAFNVRSAGRPYFNTTPIGRAVTGTLLVRA
CCHHHHHHHHHHHHHHHHHHHHCCHHEEEECCEECCCCCCCCCCCCCCCHHHHHHHEEEE
MHTDDVSIWGDGSTYKGNDIERFYRYGLLANPQLRIYKPWLDENFVAELGGRDEMSAWLT
EECCCEEEEECCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHCCCCCHHHHHHE
AHNLPYRDSKEKAYSTDANIWGATHEAKKLESLHVSIESVNPIMGVKFWDPDVKIDTEEV
ECCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHEEEHHHCCCEEEEEEECCCCEEEHHHE
TVSFEQGLPVAINGKEYDDKVALVLEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGM
EEEECCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCH
ALLHIAYERLANAIHNEDTLETYHNEGRRLGRLLYEGRWLDPQSLMIRESLTHWVASAIT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
GSVTLKLRRGWDYSILDTTGPNFSYHSEKLSMERVEGAAFGPTDRIGQLTMRNLDIADTR
CEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHH
EKLELYSAQGQLASHADLVGQLETGGASAIASNGDDDDDSQKTLDAAAMEVGVN
HHHHHHHCCCCHHHHHHHHEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVAVSKVITQLPVGERVGIAFSGGLDTSVAVAWMRENGAVPCTYTADIGQYDEPDIASVP
CCCHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCCCC
GRALDYGAEISRLVDCKAALVEEGLSAIACGAFNVRSAGRPYFNTTPIGRAVTGTLLVRA
CCHHHHHHHHHHHHHHHHHHHHCCHHEEEECCEECCCCCCCCCCCCCCCHHHHHHHEEEE
MHTDDVSIWGDGSTYKGNDIERFYRYGLLANPQLRIYKPWLDENFVAELGGRDEMSAWLT
EECCCEEEEECCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHCCCCCHHHHHHE
AHNLPYRDSKEKAYSTDANIWGATHEAKKLESLHVSIESVNPIMGVKFWDPDVKIDTEEV
ECCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHEEEHHHCCCEEEEEEECCCCEEEHHHE
TVSFEQGLPVAINGKEYDDKVALVLEANAIGGRHGLGMSDQIENRIIEAKSRGIYEAPGM
EEEECCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCH
ALLHIAYERLANAIHNEDTLETYHNEGRRLGRLLYEGRWLDPQSLMIRESLTHWVASAIT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
GSVTLKLRRGWDYSILDTTGPNFSYHSEKLSMERVEGAAFGPTDRIGQLTMRNLDIADTR
CEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHH
EKLELYSAQGQLASHADLVGQLETGGASAIASNGDDDDDSQKTLDAAAMEVGVN
HHHHHHHCCCCHHHHHHHHEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8449418 [H]