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The map label for this gene is yhcR [H]

Identifier: 50843536

GI number: 50843536

Start: 2273208

End: 2275319

Strand: Direct

Name: yhcR [H]

Synonym: PPA2097

Alternate gene names: 50843536

Gene position: 2273208-2275319 (Clockwise)

Preceding gene: 50843535

Following gene: 50843541

Centisome position: 88.79

GC content: 61.22

Gene sequence:

>2112_bases
ATGAATCACCGACGCAGGGCTCGCATTGCTGGCCTTTCGGTACTGTGCATGGCCGCGGGCAGCTTCGTTGCCAAGCCTGC
TTTCGCAGCACCGAGCACGAGTGGCACCCCGTCCGCGCCGGTCGCGTCGTGCAATGGCGACAAGAACGTCGACATCTTCA
ACTTCAACGACTTCCACGGCCGTATCGCTACCGGAGCCAACCTCTTCACCCCTGTCATCGAGGCCCGCAACGCCAACGGA
GCCGACAAGGTCCTGCTGCTCGACGCTGGCGACAACGTTGGTGGATCGACCTTCGAGTCTGGCTCCCTCAACGACGAGCC
CACCATCGACATGCTCAACGCCGCTGGTGTCGACGCCAATGCCGTCGGCAACCACGAGTTCGACAAGGGCTGGAAGGACC
TAGCCGAGCGCATCGTGCCTCATCTCAACAGCCCCTACCTCGGCGCCAACGTCTACGAAAAGGGCACCACGAAAGTAGCG
GCTCCCCTCAAAGCGTCGACGATCATCGTCAAGGATGGAGTGCGCATCGGTGTCGTCGGCGCTGTCACCCACGACCTGCC
CTCCCTGGTCTCCCCCGCCGGCATTTCCAACCTCACCATTGGTGATCCGGTTAAGGCTGTGAATGCCGAGGCCAAGCGTC
TCAAGAGCGAGGGCCTGGCCGACGTCGTCGTTGCCGAATACCACGAAGGCGCTGCCGACGGTTCCGGGGATGCCGCCAGC
CAGGGCGGCAACTTCGCACCGGTCTACGCTGACACGTCCACCGATGTTGACGTCGTCTTCAACGGCCACACTCACCAGGT
GTACTCGTGGAAGGACAAGGCCGGCGCCCCGCTCCTGCAGGCCGGCTCCTACGGCCAGCACCTGGCCAAGGTGAACGTGG
CGTGGGATTCACAGCAGAAGAAACTGTGCTCTACCGACGCCTCAATCATTGACCCGGCCACAACCCCGGCTGACGACCCA
ACGATCGCCAAGATCTCTTCGATCTACGACGATGCCAAAGCCAAGTCGGACAAAGTTGGCACTACCGTCATTGGGCATGA
AACCGCACCGATCACCACGCCGTCGAACGAGTCCGATCAGAAGCTCGGTGCCGAGGTCCGCGACCGTGAATCGACGATGA
CCAATATGGTCGCCGACATGTTCAAGGACACCTTGGGCAAAGACGACCCAAACTTCATCGGCCTACAGAACCCTGGTGGA
ACCCGCGCATCTCTGCTCGACAAGGACATCACCTATAAGCAGGCAGCCCAGGTGCTCCCCTTCGCTAATACGTTGACGAC
CACCAAGATCACCAGCGCCCAGTTCAAGAAGGTGCTTGAACAGCAGTGGCAGCGCGCCGGGACTGGCGAGGTCCCGTCAC
GTCCGTACTTGCAGCTCGGGGTGTCTTCGAACGTCACCTACACCTATGACGAATCCCGCAAAGAGGGCGACCGCATCACC
TCCGTGTGGGTGAACGGCAAACCGATCGACCCCAAGGGCACTTACACCGTAGGTTCCGGTTCTTTCCTCATCGCCGGCGG
TGACAATTTCACCGAGCTAGCCAAGGGTTCTAAGCCCGTCGATAGCGGCAAGATCGACCTGTCTGTGTGGGTGGATTGGA
TCAAGGCCCACAAGACCCTTAAGCCTGACTTCGCCAAGCGGGCTGTCTCCTTGACGACTTCGCTTCACGAGGGCATGTCC
AAGGACACCACCTTCACCCTCGGTAAACCTGCCGATAAGGCTGTTGCCCCCGACACCGTGGATTTCACCTCCAAGGATGC
TGTAGTCAACACGATCATGAAGGCCCAGATCGTCCAGGACGACAAGACCGTCGACGTCGCCACCACTCCGGTTAAGGACG
GCTGGTCCTCCGTGTCCGTCAAAGTGCCGACCGCCAAGGGGCCGGTCAGCGGTGAGGCCACCATCGTTTTCACCTTCCCG
GATTCCGGCACCACGGTGCGCTTCGCCGCCAAGCTGTCAGTACCGTCCGGCGATCATCCTGGTGGCGCTGTGATCCCGGC
CCCGAAACCGGGCCAGTCTGGCACCCCGGGCCACGACGTCAATCTAGGAACCCCCGGATCTGACAAAGGCTCCAGCAACG
GCAAACCCGGCCTGCCGAAAACTGGTGTCTGA

Upstream 100 bases:

>100_bases
AATTCGCCAGAACCATTGCCAGGACGGCCGAAAATAGACGAAGCTAGCCTTGTACGCTTTTGGGCACACAGCATCATTCG
GACGAATCCAGGGGGAACCC

Downstream 100 bases:

>100_bases
TCCGAGGATCGACACAACCTCATACTGAAAAACTCTGGCCTGAGGGGGTCCCGGGCCAGGGTTCTTGTGTGTCTGAGTTG
GGGCAGCAAAGAGATGAAGT

Product: 5'-nucleotidase/2',3'-cyclic phosphodiesterase or related esterase

Products: Phosphate; Uridine [C]

Alternate protein names: NA

Number of amino acids: Translated: 703; Mature: 703

Protein sequence:

>703_residues
MNHRRRARIAGLSVLCMAAGSFVAKPAFAAPSTSGTPSAPVASCNGDKNVDIFNFNDFHGRIATGANLFTPVIEARNANG
ADKVLLLDAGDNVGGSTFESGSLNDEPTIDMLNAAGVDANAVGNHEFDKGWKDLAERIVPHLNSPYLGANVYEKGTTKVA
APLKASTIIVKDGVRIGVVGAVTHDLPSLVSPAGISNLTIGDPVKAVNAEAKRLKSEGLADVVVAEYHEGAADGSGDAAS
QGGNFAPVYADTSTDVDVVFNGHTHQVYSWKDKAGAPLLQAGSYGQHLAKVNVAWDSQQKKLCSTDASIIDPATTPADDP
TIAKISSIYDDAKAKSDKVGTTVIGHETAPITTPSNESDQKLGAEVRDRESTMTNMVADMFKDTLGKDDPNFIGLQNPGG
TRASLLDKDITYKQAAQVLPFANTLTTTKITSAQFKKVLEQQWQRAGTGEVPSRPYLQLGVSSNVTYTYDESRKEGDRIT
SVWVNGKPIDPKGTYTVGSGSFLIAGGDNFTELAKGSKPVDSGKIDLSVWVDWIKAHKTLKPDFAKRAVSLTTSLHEGMS
KDTTFTLGKPADKAVAPDTVDFTSKDAVVNTIMKAQIVQDDKTVDVATTPVKDGWSSVSVKVPTAKGPVSGEATIVFTFP
DSGTTVRFAAKLSVPSGDHPGGAVIPAPKPGQSGTPGHDVNLGTPGSDKGSSNGKPGLPKTGV

Sequences:

>Translated_703_residues
MNHRRRARIAGLSVLCMAAGSFVAKPAFAAPSTSGTPSAPVASCNGDKNVDIFNFNDFHGRIATGANLFTPVIEARNANG
ADKVLLLDAGDNVGGSTFESGSLNDEPTIDMLNAAGVDANAVGNHEFDKGWKDLAERIVPHLNSPYLGANVYEKGTTKVA
APLKASTIIVKDGVRIGVVGAVTHDLPSLVSPAGISNLTIGDPVKAVNAEAKRLKSEGLADVVVAEYHEGAADGSGDAAS
QGGNFAPVYADTSTDVDVVFNGHTHQVYSWKDKAGAPLLQAGSYGQHLAKVNVAWDSQQKKLCSTDASIIDPATTPADDP
TIAKISSIYDDAKAKSDKVGTTVIGHETAPITTPSNESDQKLGAEVRDRESTMTNMVADMFKDTLGKDDPNFIGLQNPGG
TRASLLDKDITYKQAAQVLPFANTLTTTKITSAQFKKVLEQQWQRAGTGEVPSRPYLQLGVSSNVTYTYDESRKEGDRIT
SVWVNGKPIDPKGTYTVGSGSFLIAGGDNFTELAKGSKPVDSGKIDLSVWVDWIKAHKTLKPDFAKRAVSLTTSLHEGMS
KDTTFTLGKPADKAVAPDTVDFTSKDAVVNTIMKAQIVQDDKTVDVATTPVKDGWSSVSVKVPTAKGPVSGEATIVFTFP
DSGTTVRFAAKLSVPSGDHPGGAVIPAPKPGQSGTPGHDVNLGTPGSDKGSSNGKPGLPKTGV
>Mature_703_residues
MNHRRRARIAGLSVLCMAAGSFVAKPAFAAPSTSGTPSAPVASCNGDKNVDIFNFNDFHGRIATGANLFTPVIEARNANG
ADKVLLLDAGDNVGGSTFESGSLNDEPTIDMLNAAGVDANAVGNHEFDKGWKDLAERIVPHLNSPYLGANVYEKGTTKVA
APLKASTIIVKDGVRIGVVGAVTHDLPSLVSPAGISNLTIGDPVKAVNAEAKRLKSEGLADVVVAEYHEGAADGSGDAAS
QGGNFAPVYADTSTDVDVVFNGHTHQVYSWKDKAGAPLLQAGSYGQHLAKVNVAWDSQQKKLCSTDASIIDPATTPADDP
TIAKISSIYDDAKAKSDKVGTTVIGHETAPITTPSNESDQKLGAEVRDRESTMTNMVADMFKDTLGKDDPNFIGLQNPGG
TRASLLDKDITYKQAAQVLPFANTLTTTKITSAQFKKVLEQQWQRAGTGEVPSRPYLQLGVSSNVTYTYDESRKEGDRIT
SVWVNGKPIDPKGTYTVGSGSFLIAGGDNFTELAKGSKPVDSGKIDLSVWVDWIKAHKTLKPDFAKRAVSLTTSLHEGMS
KDTTFTLGKPADKAVAPDTVDFTSKDAVVNTIMKAQIVQDDKTVDVATTPVKDGWSSVSVKVPTAKGPVSGEATIVFTFP
DSGTTVRFAAKLSVPSGDHPGGAVIPAPKPGQSGTPGHDVNLGTPGSDKGSSNGKPGLPKTGV

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=522, Percent_Identity=27.5862068965517, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI1786687, Length=547, Percent_Identity=21.2065813528336, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1790658, Length=496, Percent_Identity=21.9758064516129, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922444, Length=524, Percent_Identity=24.8091603053435, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19922446, Length=517, Percent_Identity=23.9845261121857, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24654424, Length=517, Percent_Identity=23.9845261121857, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28573524, Length=533, Percent_Identity=26.078799249531, Blast_Score=106, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19921980, Length=432, Percent_Identity=23.3796296296296, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24652512, Length=432, Percent_Identity=23.3796296296296, Blast_Score=90, Evalue=7e-18,
Organism=Drosophila melanogaster, GI161076508, Length=432, Percent_Identity=23.3796296296296, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24641187, Length=475, Percent_Identity=23.3684210526316, Blast_Score=89, Evalue=1e-17,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.6.1.45; 3.1.3.5 [C]

Molecular weight: Translated: 73526; Mature: 73526

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHRRRARIAGLSVLCMAAGSFVAKPAFAAPSTSGTPSAPVASCNGDKNVDIFNFNDFHG
CCCCHHHHHHHHHHEEHHHCCHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCC
RIATGANLFTPVIEARNANGADKVLLLDAGDNVGGSTFESGSLNDEPTIDMLNAAGVDAN
EEECCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEHCCCCCCCC
AVGNHEFDKGWKDLAERIVPHLNSPYLGANVYEKGTTKVAAPLKASTIIVKDGVRIGVVG
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEEEECCCEEEEEE
AVTHDLPSLVSPAGISNLTIGDPVKAVNAEAKRLKSEGLADVVVAEYHEGAADGSGDAAS
HHHHHHHHHCCCCCCCCCCCCCCHHHHCHHHHHHHHCCCCEEEHHHHCCCCCCCCCCCCC
QGGNFAPVYADTSTDVDVVFNGHTHQVYSWKDKAGAPLLQAGSYGQHLAKVNVAWDSQQK
CCCCEEEEEECCCCCEEEEECCCCEEEEEECCCCCCCEEECCCCCCEEEEEEEEECCHHH
KLCSTDASIIDPATTPADDPTIAKISSIYDDAKAKSDKVGTTVIGHETAPITTPSNESDQ
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHH
KLGAEVRDRESTMTNMVADMFKDTLGKDDPNFIGLQNPGGTRASLLDKDITYKQAAQVLP
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHCCCCHHHHHHHCC
FANTLTTTKITSAQFKKVLEQQWQRAGTGEVPSRPYLQLGVSSNVTYTYDESRKEGDRIT
CCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCEEE
SVWVNGKPIDPKGTYTVGSGSFLIAGGDNFTELAKGSKPVDSGKIDLSVWVDWIKAHKTL
EEEECCCCCCCCCCEEECCCCEEEECCCCHHHHHCCCCCCCCCCEEHHHHHHHHHHHCCC
KPDFAKRAVSLTTSLHEGMSKDTTFTLGKPADKAVAPDTVDFTSKDAVVNTIMKAQIVQD
CCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DKTVDVATTPVKDGWSSVSVKVPTAKGPVSGEATIVFTFPDSGTTVRFAAKLSVPSGDHP
CCEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCC
GGAVIPAPKPGQSGTPGHDVNLGTPGSDKGSSNGKPGLPKTGV
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNHRRRARIAGLSVLCMAAGSFVAKPAFAAPSTSGTPSAPVASCNGDKNVDIFNFNDFHG
CCCCHHHHHHHHHHEEHHHCCHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCC
RIATGANLFTPVIEARNANGADKVLLLDAGDNVGGSTFESGSLNDEPTIDMLNAAGVDAN
EEECCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEHCCCCCCCC
AVGNHEFDKGWKDLAERIVPHLNSPYLGANVYEKGTTKVAAPLKASTIIVKDGVRIGVVG
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEEEECCCEEEEEE
AVTHDLPSLVSPAGISNLTIGDPVKAVNAEAKRLKSEGLADVVVAEYHEGAADGSGDAAS
HHHHHHHHHCCCCCCCCCCCCCCHHHHCHHHHHHHHCCCCEEEHHHHCCCCCCCCCCCCC
QGGNFAPVYADTSTDVDVVFNGHTHQVYSWKDKAGAPLLQAGSYGQHLAKVNVAWDSQQK
CCCCEEEEEECCCCCEEEEECCCCEEEEEECCCCCCCEEECCCCCCEEEEEEEEECCHHH
KLCSTDASIIDPATTPADDPTIAKISSIYDDAKAKSDKVGTTVIGHETAPITTPSNESDQ
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHH
KLGAEVRDRESTMTNMVADMFKDTLGKDDPNFIGLQNPGGTRASLLDKDITYKQAAQVLP
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHCCCCHHHHHHHCC
FANTLTTTKITSAQFKKVLEQQWQRAGTGEVPSRPYLQLGVSSNVTYTYDESRKEGDRIT
CCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCEEE
SVWVNGKPIDPKGTYTVGSGSFLIAGGDNFTELAKGSKPVDSGKIDLSVWVDWIKAHKTL
EEEECCCCCCCCCCEEECCCCEEEECCCCHHHHHCCCCCCCCCCEEHHHHHHHHHHHCCC
KPDFAKRAVSLTTSLHEGMSKDTTFTLGKPADKAVAPDTVDFTSKDAVVNTIMKAQIVQD
CCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DKTVDVATTPVKDGWSSVSVKVPTAKGPVSGEATIVFTFPDSGTTVRFAAKLSVPSGDHP
CCEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCC
GGAVIPAPKPGQSGTPGHDVNLGTPGSDKGSSNGKPGLPKTGV
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; UMP [C]

Specific reaction: H2O + UMP --> Phosphate + Uridine [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Inhibitor from Salmonella typhimurium cytoplasm [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]