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The map label for this gene is agaR [H]

Identifier: 50843501

GI number: 50843501

Start: 2235065

End: 2236876

Strand: Direct

Name: agaR [H]

Synonym: PPA2057

Alternate gene names: 50843501

Gene position: 2235065-2236876 (Clockwise)

Preceding gene: 50843497

Following gene: 50843502

Centisome position: 87.3

GC content: 61.31

Gene sequence:

>1812_bases
GTGGCATCCCACCTCAGCGACACCTTGCACGGGCGAACAACCGTACATAATGACGGCAAGCTCCCAGTCCATCTCACTGC
CGTCTCTATCATGTGCGCCAGTCTGCTGTCAGGAGCCGAACTCGATGACCTCGATATACTCATCGCACCCAGCGCATGGA
TGGCCGAACAACGGTGGACCCATCACCGCCTGTCCGATCTTCTGGTGGACGTCGGCACCGAATTGCATGGAGAATCCCCG
CGCGACCGCTTCGTCCTATCCTCAGAGTCCGGTTGGTCTTCAGGACGTTGGGAACCGGTCGGCTTCATTACCGACCCGGC
CTCTGGACGCGCCGTCGGCTGGCAGATCGAGCACAACGGTGGCTGGACTGTCGAATTAGCCCGGCGCTCTTCAACCCTCG
GCCTCTGCCTCTTCGGGCCGACCGACCTTCAGCACCACTGGATGCACCAGCTCAACCCTGGCGAGACTTTCACTACCCCC
GCGGTGACGTTAGTGGTATCCGATAACAGCTGGCAGGGCGCGGCAGCCGAACTAAGCACCTACCGCCGTGTATTGAGGGG
CAACAATGGCACAGCAGCTCCGATCGTCTTCAACGACTACATGAACACCCTCATGGCCGACCCTACGGCCGAGCGACTCT
CGACCCTCATCCCGGCCGCAGCTCGCACCGGAGCCGAGGTGTACTGCATCGACGCCGGATGGCACTCCGACGACACAGAC
TGGTGGGACGACGTCGGCCGCTGGGAGCCCTCGCCCCGCCGATTTCCCGGCGGGCTGCGCCACACCCTTGATCTCATCGT
CGCCTACGGCATGACGCCCGGCCTGTGGATTGAGCCGCTGGCGGTTGGTCTGCGCAGTCCTCTCGTCAATGATCTACCCC
CGGAGGCTTTCATGCGCCGCGCAGGGGTTCCGATCGTCGAACAGAATCGGGTGCGTCTAGATATGCGCAATCCGCAAGCT
CGCGCTCATCTAGATACCGTCATCGACCGGATGGTCAGTTACGGCATCGGCTACCTCAAAATTGATGACAATTTCTCTGT
CGGTTCTGGCCCCGACGAGGACGCGGATTCTCCTGGGGACGGTTTGCTGGGGCACTGCCGCGCTTGGGCCGCCTGGCTTG
CCGACCTCCCTCGACGTCACCCTGGACTTGTCGTTGAGAACTGTGCATCTGGGGGCATGACCACCGACTACGCCCTGTTG
GCCCGAGCAGCCATCCAGTCGACATCAGACCTCCAGGATTCGGTGCGTTACCCGCCCATCGCCGCCAATTCACCGATGAC
GATTTTGCCCGAACAAGCCGCCAATTGGGCTTACCCACAGCCAGACATGGACGACGAGGAAATCGTCTTTACCGTCACGA
CCTCCTTGGCCGGCACCTTTTACTTGTCCGGTCATGTCGACCGCATGAACGCCCACCAGATGGCTCTCGTGCAATCCGGC
GCAGAGGTAGCCAAGGCCTTACGGTCAGATCTGACCAGCCAAATACCGTGGTGGCCGAAAGGCCTTGCCGGCTGGGACGA
CGAGTGGGTCGTCACCGGGCGTCGAAAAAAGTCCTCTCGCGACGGGGTGATCATTGTCTGGCACCGTCCTAGCACCGAAT
CGACGGTTCTCGACTTACCTGACCTGGCTGGGACCCACCTCGAACCCCTTTACCCACCTGCCTCCCTGGTCCCCGACCCA
GGAATTCACATTGCTGACTCATCGGGTTCACCTCGGCTGAGTTGTCCGTCTCCCAAACCAACCGGCCGGGTCTTCCGCGT
CCGGGACCTTCACGATTGCGTCACCGATGTCGCAGAAATGGAGAAGAAATGA

Upstream 100 bases:

>100_bases
GTCTGCGTCCCGTTCGCCGCGAACCGCTCACCTCGCCGTGGCCCGGCACCCGCGTCATCCAGCATGATCGAGGATCCGAC
CTCACCGTCACCGTCGATCT

Downstream 100 bases:

>100_bases
ATCGCACCCTCAAGGTCGCCGCAGTAGGAGCCATCGCGATATTGTGCCTCGCTGCCTGCTCCGACCCAGGCTCAGACTCG
GCGCAATCCTCAGGACCGGA

Product: alpha-galactosidase

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MASHLSDTLHGRTTVHNDGKLPVHLTAVSIMCASLLSGAELDDLDILIAPSAWMAEQRWTHHRLSDLLVDVGTELHGESP
RDRFVLSSESGWSSGRWEPVGFITDPASGRAVGWQIEHNGGWTVELARRSSTLGLCLFGPTDLQHHWMHQLNPGETFTTP
AVTLVVSDNSWQGAAAELSTYRRVLRGNNGTAAPIVFNDYMNTLMADPTAERLSTLIPAAARTGAEVYCIDAGWHSDDTD
WWDDVGRWEPSPRRFPGGLRHTLDLIVAYGMTPGLWIEPLAVGLRSPLVNDLPPEAFMRRAGVPIVEQNRVRLDMRNPQA
RAHLDTVIDRMVSYGIGYLKIDDNFSVGSGPDEDADSPGDGLLGHCRAWAAWLADLPRRHPGLVVENCASGGMTTDYALL
ARAAIQSTSDLQDSVRYPPIAANSPMTILPEQAANWAYPQPDMDDEEIVFTVTTSLAGTFYLSGHVDRMNAHQMALVQSG
AEVAKALRSDLTSQIPWWPKGLAGWDDEWVVTGRRKKSSRDGVIIVWHRPSTESTVLDLPDLAGTHLEPLYPPASLVPDP
GIHIADSSGSPRLSCPSPKPTGRVFRVRDLHDCVTDVAEMEKK

Sequences:

>Translated_603_residues
MASHLSDTLHGRTTVHNDGKLPVHLTAVSIMCASLLSGAELDDLDILIAPSAWMAEQRWTHHRLSDLLVDVGTELHGESP
RDRFVLSSESGWSSGRWEPVGFITDPASGRAVGWQIEHNGGWTVELARRSSTLGLCLFGPTDLQHHWMHQLNPGETFTTP
AVTLVVSDNSWQGAAAELSTYRRVLRGNNGTAAPIVFNDYMNTLMADPTAERLSTLIPAAARTGAEVYCIDAGWHSDDTD
WWDDVGRWEPSPRRFPGGLRHTLDLIVAYGMTPGLWIEPLAVGLRSPLVNDLPPEAFMRRAGVPIVEQNRVRLDMRNPQA
RAHLDTVIDRMVSYGIGYLKIDDNFSVGSGPDEDADSPGDGLLGHCRAWAAWLADLPRRHPGLVVENCASGGMTTDYALL
ARAAIQSTSDLQDSVRYPPIAANSPMTILPEQAANWAYPQPDMDDEEIVFTVTTSLAGTFYLSGHVDRMNAHQMALVQSG
AEVAKALRSDLTSQIPWWPKGLAGWDDEWVVTGRRKKSSRDGVIIVWHRPSTESTVLDLPDLAGTHLEPLYPPASLVPDP
GIHIADSSGSPRLSCPSPKPTGRVFRVRDLHDCVTDVAEMEKK
>Mature_602_residues
ASHLSDTLHGRTTVHNDGKLPVHLTAVSIMCASLLSGAELDDLDILIAPSAWMAEQRWTHHRLSDLLVDVGTELHGESPR
DRFVLSSESGWSSGRWEPVGFITDPASGRAVGWQIEHNGGWTVELARRSSTLGLCLFGPTDLQHHWMHQLNPGETFTTPA
VTLVVSDNSWQGAAAELSTYRRVLRGNNGTAAPIVFNDYMNTLMADPTAERLSTLIPAAARTGAEVYCIDAGWHSDDTDW
WDDVGRWEPSPRRFPGGLRHTLDLIVAYGMTPGLWIEPLAVGLRSPLVNDLPPEAFMRRAGVPIVEQNRVRLDMRNPQAR
AHLDTVIDRMVSYGIGYLKIDDNFSVGSGPDEDADSPGDGLLGHCRAWAAWLADLPRRHPGLVVENCASGGMTTDYALLA
RAAIQSTSDLQDSVRYPPIAANSPMTILPEQAANWAYPQPDMDDEEIVFTVTTSLAGTFYLSGHVDRMNAHQMALVQSGA
EVAKALRSDLTSQIPWWPKGLAGWDDEWVVTGRRKKSSRDGVIIVWHRPSTESTVLDLPDLAGTHLEPLYPPASLVPDPG
IHIADSSGSPRLSCPSPKPTGRVFRVRDLHDCVTDVAEMEKK

Specific function: Alpha-galactosidase associated with the raffinose operon [H]

COG id: COG3345

COG function: function code G; Alpha-galactosidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 36 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002252
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 66095; Mature: 65964

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASHLSDTLHGRTTVHNDGKLPVHLTAVSIMCASLLSGAELDDLDILIAPSAWMAEQRWT
CCCCHHHHHCCCEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
HHRLSDLLVDVGTELHGESPRDRFVLSSESGWSSGRWEPVGFITDPASGRAVGWQIEHNG
HHHHHHHHHHHCHHHCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCEEEEEEECCC
GWTVELARRSSTLGLCLFGPTDLQHHWMHQLNPGETFTTPAVTLVVSDNSWQGAAAELST
CEEEEEEECCCCEEEEEECCCCHHHHHHHHCCCCCEECCCEEEEEEECCCCCCHHHHHHH
YRRVLRGNNGTAAPIVFNDYMNTLMADPTAERLSTLIPAAARTGAEVYCIDAGWHSDDTD
HHHHHHCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
WWDDVGRWEPSPRRFPGGLRHTLDLIVAYGMTPGLWIEPLAVGLRSPLVNDLPPEAFMRR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHH
AGVPIVEQNRVRLDMRNPQARAHLDTVIDRMVSYGIGYLKIDDNFSVGSGPDEDADSPGD
CCCCEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCC
GLLGHCRAWAAWLADLPRRHPGLVVENCASGGMTTDYALLARAAIQSTSDLQDSVRYPPI
CHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
AANSPMTILPEQAANWAYPQPDMDDEEIVFTVTTSLAGTFYLSGHVDRMNAHQMALVQSG
CCCCCCEEECHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCC
AEVAKALRSDLTSQIPWWPKGLAGWDDEWVVTGRRKKSSRDGVIIVWHRPSTESTVLDLP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCEEEECC
DLAGTHLEPLYPPASLVPDPGIHIADSSGSPRLSCPSPKPTGRVFRVRDLHDCVTDVAEM
CCCCCCCCCCCCCHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHH
EKK
CCC
>Mature Secondary Structure 
ASHLSDTLHGRTTVHNDGKLPVHLTAVSIMCASLLSGAELDDLDILIAPSAWMAEQRWT
CCCHHHHHCCCEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
HHRLSDLLVDVGTELHGESPRDRFVLSSESGWSSGRWEPVGFITDPASGRAVGWQIEHNG
HHHHHHHHHHHCHHHCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCEEEEEEECCC
GWTVELARRSSTLGLCLFGPTDLQHHWMHQLNPGETFTTPAVTLVVSDNSWQGAAAELST
CEEEEEEECCCCEEEEEECCCCHHHHHHHHCCCCCEECCCEEEEEEECCCCCCHHHHHHH
YRRVLRGNNGTAAPIVFNDYMNTLMADPTAERLSTLIPAAARTGAEVYCIDAGWHSDDTD
HHHHHHCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
WWDDVGRWEPSPRRFPGGLRHTLDLIVAYGMTPGLWIEPLAVGLRSPLVNDLPPEAFMRR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHH
AGVPIVEQNRVRLDMRNPQARAHLDTVIDRMVSYGIGYLKIDDNFSVGSGPDEDADSPGD
CCCCEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCC
GLLGHCRAWAAWLADLPRRHPGLVVENCASGGMTTDYALLARAAIQSTSDLQDSVRYPPI
CHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
AANSPMTILPEQAANWAYPQPDMDDEEIVFTVTTSLAGTFYLSGHVDRMNAHQMALVQSG
CCCCCCEEECHHHCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCC
AEVAKALRSDLTSQIPWWPKGLAGWDDEWVVTGRRKKSSRDGVIIVWHRPSTESTVLDLP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCEEEECC
DLAGTHLEPLYPPASLVPDPGIHIADSSGSPRLSCPSPKPTGRVFRVRDLHDCVTDVAEM
CCCCCCCCCCCCCHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHH
EKK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA